Rorug05G0188100

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000301
Physical Location & Seq
Reverse (-)
18275100 .. 18280240
5141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0188100.1

Sequence Viewer

Length: 1815 bp
ATGGACCGGACTAGATTTAATATGAGGATGCGTTGTTCTGGTTCAACACCATCAGAGGAGTCAGCTTTAGATCTCGAGAGAACCTGTTGTAGTCATTCTAATTTGCCTTCCTTAAGTCCGCCAACACTTCAACCCTTTGCATCAGCTGGACAGCATTGTGAGACTAGTGCTGCGTACTTCTCCTGGCCCACTTCAAGTCGATTGAATGATGCAGCTGAAGAGAGGGCAAATTATTTTACTAATCTACAGAAAGGTGTCCTGCCTGAAACTCTTGGTCAATTGCCAAAAGGGCAGCAAGCGACTACATTGCTTGAACTGATGACTATAAGGGCATTTCATAGCAAGATTTTGCGGTGTTACAGTCTTGGAACAGCAATTGGCTTTCGCATTCGACGGGGTGTGTTAACAGATATACCAGCTATCCTTGTGTTTGTTTCAAGAAAAGTTCACAAGCAATGGCTCAGCCCAATACAATGTCTACCTACTGCTCTAGAGGGGCCTGGAGGTGTATGGTGTGATGTGGATGTGGTAGAATTCTCATATTTTGGTGCACCCGAGCCTGCTCCCAAAGAACAATTGTACACTGAAATTGTAGATGATTTGCGTGGTGGTGATCTATGCATTGGCTCAGGTTCCCAGGTGGCAAGCCAGGAGACATATGGGACTTTGGGTGCTATAGTTAAAAGCCAAACTGGGAACAGACAAGTTGGTTTTCTCACAAATCGCCATGTTGCAGTTGACTTAGATTACCCAAATCAGAAAATGTTCCATCCCCTGCCCCCTTCACTTGGACCTGGTGTATACCTTGGTGCTGTAGAGAGAGCTACCTCATTTATCACAGATGAGCTCTGGTATGGCATTTTTGCTGGAATTAACCCAGAGACATTTGTGAGAGCAGACGGGGCATTTATCCCATTTTCTGATGATTTTGACATGTCTACTGTTACTACATCTGTGAAAGGTGTAGGATGGATTGGTGATGTTAAGATAATAGACTTGCAGTCTCCGATCAGTACCCTTATCGGAAAGCATGTGATGAAGGTTGGAAGAAGTTCTGGCTTGACAGCTGGAACTGTATTGGCCTATGCCCTTGAGTACAATGATGAGAAAGGCATATGTTTCTTGACTGATTTCCTTGTTGTCGGCGAGAACCAGCAGACGTTTGACCTTGAAGGAGACAGTGGAAGTCTCATAACACTAAAAGGTGAGAATGGTGAGAAGCCACGGCCGATTGGGATCATATGGGGTGGGACTGCAAACCGAGGCCGTCTTAAATTAAAAATTGGTCAACCTCCTGAGAATTGGACAAGTGGAGTTGATCTCGGGCGTCTTCTCAATCTCCTTGAACTTGACCTCATTACAACTGATGAAGGGCTAAAAGTGGCTGTACAAGAACAGAGGAGCGTGTCAGCAACTGCAATTGGATCCACTGTTGGTGACTCGTCACCTCCTGATGGGTTACTTCCAAAGGAAGGGCCTGAGGAGAAGTTCGAGTCACTGGGTCTCCAAATCCAGCGTATCCCTTTAGAAGCTGAACCCGGAAGCCTGCCTGTGAGTCCGTCATTAGTAGAAACAGAGTTCCATCTAGAAGATGGGATCAAGGTGGTTCCTAGTGTTGAGCATCAATTCATACCAAGCTTCATCAGAGGTTCCCCACTACATAATATGAATCAGATGGGCAGAACAGTTTCTGAGAATCTCTCATCATTAAGGAATGGCTGCGACGAAGATATTTGTTTTTCACTGCAGTTGGGCGACAATGAGGCCAAGAGAAGACGTTCTGATACTTCAACCAGTACTGTAGAACCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

604

Amino Acids

65.7

Weight (kDa)

5.1

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAL1_N PF25608 98 - 177 2.1e-44 NARROW LEAF 1 N-terminal domain
Nal1_C PF25819 190 - 457 8.6e-158 Nal1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 478, 801, 938
AciI CCGC 2 cut(s) 119, 352
AclWI GGATC 4 cut(s) 1244, 1419, 1432, 1604
AcoI YGGCCR 1 cut(s) 1226
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 1327
AfaI GTAC 6 cut(s) 176, 581, 1015, 1097, 1389, 1798
AfiI CCNNNNNNNGG 3 cut(s) 788, 1454, 1472
AflII CTTAAG 1 cut(s) 112
AflIII ACRYGT 1 cut(s) 933
AgsI TTSAA 9 cut(s) 45, 131, 195, 205, 314, 438, 1172, 1346, 1791
AhdI GACNNNNNGTC 1 cut(s) 1000
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 5 cut(s) 182, 499, 636, 648, 793
AloI GAACNNNNNNTCC 2 cut(s) 19, 51
AluBI AGCT 9 cut(s) 65, 146, 215, 419, 824, 847, 1067, 1532, 1638
AluI AGCT 9 cut(s) 65, 146, 215, 419, 824, 847, 1067, 1532, 1638
Alw21I GWGCWC 2 cut(s) 553, 849
Alw26I GTCTC 7 cut(s) 155, 647, 875, 1008, 1170, 1193, 1508
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 4 cut(s) 1244, 1419, 1432, 1604
AlwNI CAGNNNCTG 2 cut(s) 1415, 1691
Ama87I CYCGRG 3 cut(s) 74, 554, 1322
AoxI GGCC 7 cut(s) 185, 497, 1080, 1226, 1264, 1475, 1764
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 4 cut(s) 170, 212, 292, 1719
ApoI RAATTY 1 cut(s) 533
Asp700I GAANNNNTTC 4 cut(s) 764, 1051, 1687, 1777
AspS9I GGNCC 5 cut(s) 4, 186, 497, 791, 1475
AsuC2I CCSGG 1 cut(s) 1539
AsuHPI GGTGA 6 cut(s) 623, 989, 1217, 1226, 1437, 1448
AvaI CYCGRG 3 cut(s) 74, 554, 1322
AvaII GGWCC 2 cut(s) 4, 791
AxyI CCTNAGG 1 cut(s) 1479
BaeGI GKGCMC 1 cut(s) 553
BamHI GGATCC 1 cut(s) 1424
BanII GRGCYC 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 1322, 1780
Bbv12I GWGCWC 2 cut(s) 553, 849
BbvI GCAGC 4 cut(s) 157, 224, 304, 1706
BccI CCATC 7 cut(s) 58, 777, 963, 1448, 1586, 1590, 1669
BceAI ACGGC 2 cut(s) 1241, 1251
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 5 cut(s) 184, 501, 638, 650, 795
BciVI GTATCC 1 cut(s) 1529
BcnI CCSGG 1 cut(s) 1539
BcoDI GTCTC 7 cut(s) 155, 647, 875, 1008, 1170, 1193, 1508
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 5 cut(s) 12, 165, 491, 1586, 1611
BfmI CTRYAG 5 cut(s) 245, 675, 813, 1745, 1800
BfrI CTTAAG 1 cut(s) 112
BfuI GTATCC 1 cut(s) 1529
BglI GCCNNNNNGGC 1 cut(s) 289
BglII AGATCT 1 cut(s) 70
BisI GCNGC 4 cut(s) 171, 213, 293, 1720
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 4 cut(s) 172, 214, 294, 1721
BmcAI AGTACT 1 cut(s) 1798
Bme1390I CCNGG 6 cut(s) 184, 501, 638, 650, 795, 1539
Bme18I GGWCC 2 cut(s) 4, 791
BmeRI GACNNNNNGTC 1 cut(s) 1000
BmeT110I CYCGRG 3 cut(s) 74, 554, 1322
BmgT120I GGNCC 5 cut(s) 4, 186, 497, 791, 1475
BmiI GGNNCC 5 cut(s) 498, 634, 1426, 1608, 1651
BmrFI CCNGG 6 cut(s) 184, 501, 638, 650, 795, 1539
BmrI ACTGGG 2 cut(s) 702, 1508
BmsI GCATC 4 cut(s) 18, 149, 199, 1630
BmuI ACTGGG 2 cut(s) 702, 1508
BpiI GAAGAC 2 cut(s) 1322, 1780
BplI GAGNNNNNCTC 4 cut(s) 1305, 1337, 1685, 1717
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 628
Bpu1102I GCTNAGC 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 1112
BpuMI CCSGG 1 cut(s) 1539
BsaBI GATNNNNATC 1 cut(s) 1235
BsaHI GRCGYC 1 cut(s) 1327
BsaI GGTCTC 1 cut(s) 1508
BsaJI CCNNGG 4 cut(s) 636, 805, 1223, 1261
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 1488, 1518
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 1454, 1472
Bse1I ACTGG 3 cut(s) 697, 1503, 1794
Bse21I CCTNAGG 1 cut(s) 1479
Bse3DI GCAATG 2 cut(s) 305, 461
Bse8I GATNNNNATC 1 cut(s) 1235
BseBI CCWGG 5 cut(s) 184, 501, 638, 650, 795
BseDI CCNNGG 4 cut(s) 636, 805, 1223, 1261
BseGI GGATG 4 cut(s) 33, 529, 769, 974
BseJI GATNNNNATC 1 cut(s) 1235
BseLI CCNNNNNNNGG 3 cut(s) 788, 1454, 1472
BseMI GCAATG 2 cut(s) 305, 461
BseMII CTCAG 5 cut(s) 475, 642, 1287, 1470, 1683
BseNI ACTGG 3 cut(s) 697, 1503, 1794
BseRI GAGGAG 3 cut(s) 71, 1414, 1496
BseSI GKGCMC 1 cut(s) 553
BseX3I CGGCCG 1 cut(s) 1226
BseXI GCAGC 4 cut(s) 157, 224, 304, 1706
Bsh1285I CGRYCG 1 cut(s) 1229
BshFI GGCC 7 cut(s) 187, 499, 1082, 1228, 1266, 1477, 1766
BsiEI CGRYCG 1 cut(s) 1229
BsiHKAI GWGCWC 2 cut(s) 553, 849
BsiHKCI CYCGRG 3 cut(s) 74, 554, 1322
BsiSI CCGG 2 cut(s) 7, 1539
BslFI GGGAC 2 cut(s) 676, 1264
BslI CCNNNNNNNGG 3 cut(s) 788, 1454, 1472
BsmAI GTCTC 7 cut(s) 155, 647, 875, 1008, 1170, 1193, 1508
BsmFI GGGAC 2 cut(s) 676, 1264
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 7 cut(s) 187, 499, 1082, 1228, 1266, 1477, 1766
Bso31I GGTCTC 1 cut(s) 1508
BsoBI CYCGRG 3 cut(s) 74, 554, 1322
Bsp1286I GDGCHC 2 cut(s) 553, 849
Bsp1407I TGTACA 2 cut(s) 579, 1387
Bsp143I GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
Bsp1720I GCTNAGC 1 cut(s) 461
BspACI CCGC 2 cut(s) 119, 352
BspANI GGCC 7 cut(s) 187, 499, 1082, 1228, 1266, 1477, 1766
BspCNI CTCAG 5 cut(s) 474, 641, 1288, 1471, 1684
BspLI GGNNCC 5 cut(s) 498, 634, 1426, 1608, 1651
BspMAI CTGCAG 1 cut(s) 1749
BspPI GGATC 4 cut(s) 1244, 1419, 1432, 1604
BspTI CTTAAG 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 1508
BsrDI GCAATG 2 cut(s) 305, 461
BsrGI TGTACA 2 cut(s) 579, 1387
BsrI ACTGG 3 cut(s) 697, 1503, 1794
BssECI CCNNGG 4 cut(s) 636, 805, 1223, 1261
BssMI GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
BssNAI GTATAC 1 cut(s) 802
BssNI GRCGYC 1 cut(s) 1327
BssT1I CCWWGG 1 cut(s) 805
Bst1107I GTATAC 1 cut(s) 802
Bst2UI CCWGG 5 cut(s) 184, 501, 638, 650, 795
Bst4CI ACNGT 7 cut(s) 362, 943, 1075, 1181, 1432, 1687, 1801
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 1327
BstAFI CTTAAG 1 cut(s) 112
BstAUI TGTACA 2 cut(s) 579, 1387
BstC8I GCNNGC 4 cut(s) 297, 561, 646, 1547
BstDEI CTNAG 6 cut(s) 461, 628, 742, 1296, 1479, 1692
BstDSI CCRYGG 1 cut(s) 1223
BstF5I GGATG 4 cut(s) 33, 529, 769, 974
BstKTI GATC 7 cut(s) 73, 616, 1011, 1239, 1321, 1427, 1599
BstMAI GTCTC 7 cut(s) 155, 647, 875, 1008, 1170, 1193, 1508
BstMBI GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
BstMCI CGRYCG 1 cut(s) 1229
BstMWI GCNNNNNNNGC 2 cut(s) 289, 902
BstNI CCWGG 5 cut(s) 184, 501, 638, 650, 795
BstNSI RCATGY 2 cut(s) 937, 1034
BstSCI CCNGG 6 cut(s) 182, 499, 636, 648, 793, 1537
BstSFI CTRYAG 5 cut(s) 245, 675, 813, 1745, 1800
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 4 cut(s) 157, 224, 304, 1706
BstV2I GAAGAC 2 cut(s) 1322, 1780
BstX2I RGATCY 2 cut(s) 70, 1424
BstYI RGATCY 2 cut(s) 70, 1424
BstZ17I GTATAC 1 cut(s) 802
BstZI CGGCCG 1 cut(s) 1226
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 1 cut(s) 1529
BsuRI GGCC 7 cut(s) 187, 499, 1082, 1228, 1266, 1477, 1766
BtgI CCRYGG 1 cut(s) 1223
BtsCI GGATG 4 cut(s) 33, 529, 769, 974
BtsI GCAGTG 1 cut(s) 1742
BtsIMutI CAGTG 5 cut(s) 582, 1186, 1428, 1496, 1742
Cac8I GCNNGC 4 cut(s) 297, 561, 646, 1547
CaiI CAGNNNCTG 2 cut(s) 1415, 1691
Cfr13I GGNCC 5 cut(s) 4, 186, 497, 791, 1475
CseI GACGC 1 cut(s) 1316
CsiI ACCWGGT 1 cut(s) 793
Csp6I GTAC 6 cut(s) 175, 580, 1014, 1096, 1388, 1797
CviAII CATG 3 cut(s) 728, 934, 1031
CviQI GTAC 6 cut(s) 175, 580, 1014, 1096, 1388, 1797
DdeI CTNAG 6 cut(s) 461, 628, 742, 1296, 1479, 1692
DpnI GATC 7 cut(s) 72, 615, 1010, 1238, 1320, 1426, 1598
DpnII GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
DriI GACNNNNNGTC 1 cut(s) 1000
EaeI YGGCCR 1 cut(s) 1226
EagI CGGCCG 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 1000
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 847
EclXI CGGCCG 1 cut(s) 1226
Eco130I CCWWGG 1 cut(s) 805
Eco24I GRGCYC 1 cut(s) 849
Eco31I GGTCTC 1 cut(s) 1508
Eco47I GGWCC 2 cut(s) 4, 791
Eco52I CGGCCG 1 cut(s) 1226
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 3 cut(s) 74, 554, 1322
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 2 cut(s) 497, 1475
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 5 cut(s) 182, 499, 636, 648, 793
EcoT14I CCWWGG 1 cut(s) 805
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 849
ErhI CCWWGG 1 cut(s) 805
FaeI CATG 3 cut(s) 731, 937, 1034
FaqI GGGAC 2 cut(s) 676, 1264
FatI CATG 3 cut(s) 727, 933, 1030
FauNDI CATATG 3 cut(s) 658, 1115, 1241
FblI GTMKAC 3 cut(s) 478, 801, 938
Fnu4HI GCNGC 4 cut(s) 171, 213, 293, 1720
FokI GGATG 4 cut(s) 40, 536, 756, 981
FriOI GRGCYC 1 cut(s) 849
Fsp4HI GCNGC 4 cut(s) 171, 213, 293, 1720
FspBI CTAG 5 cut(s) 12, 165, 491, 1586, 1611
GluI GCNGC 4 cut(s) 171, 213, 293, 1720
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 7 cut(s) 187, 499, 1082, 1228, 1266, 1477, 1766
HapII CCGG 2 cut(s) 7, 1539
HgaI GACGC 1 cut(s) 1316
Hin1I GRCGYC 1 cut(s) 1327
Hin1II CATG 3 cut(s) 731, 937, 1034
HincII GTYRAC 3 cut(s) 405, 739, 1289
HindII GTYRAC 3 cut(s) 405, 739, 1289
HindIII AAGCTT 1 cut(s) 1636
HinfI GANTC 6 cut(s) 59, 1439, 1493, 1555, 1669, 1696
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 2 cut(s) 7, 1539
HphI GGTGA 6 cut(s) 623, 989, 1217, 1226, 1437, 1448
Hpy166II GTNNAC 9 cut(s) 405, 448, 479, 551, 582, 739, 802, 939, 1289
Hpy188I TCNGA 9 cut(s) 55, 759, 922, 1008, 1025, 1646, 1674, 1693, 1783
Hpy188III TCNNGA 8 cut(s) 74, 76, 438, 491, 1123, 1295, 1451, 1586
Hpy8I GTNNAC 9 cut(s) 405, 448, 479, 551, 582, 739, 802, 939, 1289
Hpy99I CGWCG 2 cut(s) 396, 1727
HpyAV CCTTC 6 cut(s) 117, 792, 1033, 1166, 1364, 1466
HpyCH4III ACNGT 7 cut(s) 362, 943, 1075, 1181, 1432, 1687, 1801
HpyCH4IV ACGT 2 cut(s) 1160, 1777
HpyCH4V TGCA 9 cut(s) 140, 212, 551, 621, 734, 1000, 1256, 1418, 1747
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 902
HpyF3I CTNAG 6 cut(s) 461, 628, 742, 1296, 1479, 1692
HpySE526I ACGT 2 cut(s) 1160, 1777
Hsp92I GRCGYC 1 cut(s) 1327
Hsp92II CATG 3 cut(s) 731, 937, 1034
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
LmnI GCTCC 2 cut(s) 568, 1401
Lsp1109I GCAGC 4 cut(s) 157, 224, 304, 1706
LweI GCATC 4 cut(s) 18, 149, 199, 1630
MabI ACCWGGT 1 cut(s) 793
MaeI CTAG 5 cut(s) 12, 165, 491, 1586, 1611
MaeII ACGT 2 cut(s) 1160, 1777
MaeIII GTNAC 6 cut(s) 356, 943, 1436, 1443, 1458, 1494
MalI GATC 7 cut(s) 72, 615, 1010, 1238, 1320, 1426, 1598
MboI GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
MboII GAAGA 6 cut(s) 230, 1059, 1322, 1601, 1739, 1785
MfeI CAATTG 4 cut(s) 278, 375, 575, 1419
MflI RGATCY 2 cut(s) 70, 1424
MhlI GDGCHC 2 cut(s) 553, 849
MlyI GAGTC 4 cut(s) 68, 1433, 1502, 1564
MmeI TCCRAC 1 cut(s) 1024
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 4 cut(s) 764, 1051, 1687, 1777
MseI TTAA 9 cut(s) 18, 113, 404, 681, 873, 984, 1272, 1277, 1709
MspA1I CMGCKG 3 cut(s) 146, 215, 1067
MspCI CTTAAG 1 cut(s) 112
MspI CCGG 2 cut(s) 7, 1539
MspR9I CCNGG 6 cut(s) 184, 501, 638, 650, 795, 1539
MunI CAATTG 4 cut(s) 278, 375, 575, 1419
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 5 cut(s) 184, 501, 638, 650, 795
MwoI GCNNNNNNNGC 2 cut(s) 289, 902
NciI CCSGG 1 cut(s) 1539
NdeI CATATG 3 cut(s) 658, 1115, 1241
NdeII GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
NlaIII CATG 3 cut(s) 731, 937, 1034
NlaIV GGNNCC 5 cut(s) 498, 634, 1426, 1608, 1651
NmuCI GTSAC 3 cut(s) 1436, 1443, 1494
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 2 cut(s) 937, 1034
PaeR7I CTCGAG 1 cut(s) 74
PciI ACATGT 1 cut(s) 933
PctI GAATGC 1 cut(s) 387
PdmI GAANNNNTTC 4 cut(s) 764, 1051, 1687, 1777
PfeI GAWTC 2 cut(s) 1669, 1696
PkrI GCNGC 4 cut(s) 172, 214, 294, 1721
PleI GAGTC 4 cut(s) 67, 1433, 1501, 1563
PpsI GAGTC 4 cut(s) 67, 1433, 1501, 1563
PscI ACATGT 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 5 cut(s) 182, 499, 636, 648, 793
PspGI CCWGG 5 cut(s) 182, 499, 636, 648, 793
PspN4I GGNNCC 5 cut(s) 498, 634, 1426, 1608, 1651
PspPI GGNCC 5 cut(s) 4, 186, 497, 791, 1475
PstI CTGCAG 1 cut(s) 1749
PstNI CAGNNNCTG 2 cut(s) 1415, 1691
PsuI RGATCY 2 cut(s) 70, 1424
PvuII CAGCTG 3 cut(s) 146, 215, 1067
RsaI GTAC 6 cut(s) 176, 581, 1015, 1097, 1389, 1798
RsaNI GTAC 6 cut(s) 175, 580, 1014, 1096, 1388, 1797
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 9 cut(s) 18, 113, 404, 681, 873, 984, 1272, 1277, 1709
SatI GCNGC 4 cut(s) 171, 213, 293, 1720
Sau3AI GATC 7 cut(s) 70, 613, 1008, 1236, 1318, 1424, 1596
Sau96I GGNCC 5 cut(s) 4, 186, 497, 791, 1475
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 4 cut(s) 68, 1433, 1502, 1564
ScrFI CCNGG 6 cut(s) 184, 501, 638, 650, 795, 1539
SduI GDGCHC 2 cut(s) 553, 849
SexAI ACCWGGT 1 cut(s) 793
SfaNI GCATC 4 cut(s) 18, 149, 199, 1630
SfcI CTRYAG 5 cut(s) 245, 675, 813, 1745, 1800
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 2 cut(s) 4, 791
SlaI CTCGAG 1 cut(s) 74
SmlI CTYRAG 3 cut(s) 74, 112, 1091
SmoI CTYRAG 3 cut(s) 74, 112, 1091
SpeI ACTAGT 1 cut(s) 164
SsiI CCGC 2 cut(s) 119, 352
SspMI CTAG 5 cut(s) 12, 165, 491, 1586, 1611
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 6 cut(s) 182, 499, 636, 648, 793, 1537
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 7 cut(s) 362, 943, 1075, 1181, 1432, 1687, 1801
TaiI ACGT 2 cut(s) 1163, 1780
TaqI TCGA 4 cut(s) 75, 199, 391, 1491
TatI WGTACW 4 cut(s) 579, 1095, 1387, 1796
TfiI GAWTC 2 cut(s) 1669, 1696
Tru1I TTAA 9 cut(s) 18, 113, 404, 681, 873, 984, 1272, 1277, 1709
Tru9I TTAA 9 cut(s) 18, 113, 404, 681, 873, 984, 1272, 1277, 1709
TscAI CASTG 5 cut(s) 589, 1186, 1435, 1503, 1749
TseFI GTSAC 3 cut(s) 1436, 1443, 1494
TseI GCWGC 4 cut(s) 170, 212, 292, 1719
Tsp45I GTSAC 3 cut(s) 1436, 1443, 1494
TspDTI ATGAA 6 cut(s) 326, 1052, 1383, 1618, 1630, 1682
TspGWI ACGGA 1 cut(s) 1548
TspRI CASTG 5 cut(s) 589, 1186, 1435, 1503, 1749
Vha464I CTTAAG 1 cut(s) 112
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 2 cut(s) 4, 791
XapI RAATTY 1 cut(s) 533
XbaI TCTAGA 2 cut(s) 490, 1585
XceI RCATGY 2 cut(s) 937, 1034
XcmI CCANNNNNNNNNTGG 2 cut(s) 656, 1589
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 3 cut(s) 478, 801, 938
XmnI GAANNNNTTC 4 cut(s) 764, 1051, 1687, 1777
XspI CTAG 5 cut(s) 12, 165, 491, 1586, 1611
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.