Rorug04G0396600

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000301
Physical Location & Seq
Reverse (-)
56281075 .. 56282258
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0396600.1

Sequence Viewer

Length: 579 bp
ATGAAGGCAGCAACAGTAGCAGCAGGACCAGAACTACTCGAGGGTAGCGAAGCCCACAAACCAATCCCAAAAGTGAGGGTGAGCAGAGTGCTCTCTGTTATCGATTTCATTCTGCGAGTAGTTGCAGCCGTCGGAACACTAGCAAGTGCCATAGCCATGGGAACCAGCAGGCAAACACTTCCCTTTGTCACACGGTTTGTTCGATTCAAGGCTAATTACAAGGATCTTCCCACGTTCACGTTCTTTGTGATTGTCAATTCAATTGTATGTGTCTACCTTGTGCTTTCACTTCCGGTGTCTATTGTCCACATAATCTGGACTGGAGCAGTGAAAAGTAGGATCACCTTGGTCATCTTAGACACGGCAATGCTGGGTCTTTTAACTGCTGGTGCATCCTCAGCAACAGCAATAGTGGCCATGGCACACTATGGCAGCACCCTTGCCAATTGGTTTCCATTCTGCCGCCAATTCAACAACTTCTGTCAGCGCATTTCGGGCTCTCTGATCGGCTCGTTCGTCGCCATTGTTGTGATCATACTGCTAATCATCTTGTCCTCTGTCGCAATCTCTCGCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

192

Amino Acids

20.67

Weight (kDa)

10.48

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 177 5.4e-44 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AciI CCGC 2 cut(s) 463, 574
AclWI GGATC 2 cut(s) 231, 347
AcoI YGGCCR 1 cut(s) 414
AgsI TTSAA 3 cut(s) 208, 261, 472
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 2 cut(s) 231, 347
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 4 cut(s) 8, 20, 125, 432
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 91, 334
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 397
BbvI GCAGC 4 cut(s) 20, 32, 137, 444
BceAI ACGGC 2 cut(s) 113, 378
BclI TGATCA 1 cut(s) 531
BfaI CTAG 2 cut(s) 140, 577
BisI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
BlsI GCNGC 6 cut(s) 10, 22, 127, 434, 464, 575
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 397
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 156, 345, 417
BsaWI WCCGGW 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 372
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 3 cut(s) 156, 345, 417
BseGI GGATG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 325
BseXI GCAGC 4 cut(s) 20, 32, 137, 444
BseYI CCCAGC 1 cut(s) 370
BshFI GGCC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 293
BsnI GGCC 1 cut(s) 416
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 93, 500
Bsp143I GATC 4 cut(s) 223, 339, 504, 531
Bsp19I CCATGG 2 cut(s) 156, 417
BspACI CCGC 2 cut(s) 463, 574
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 1 cut(s) 410
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 2 cut(s) 231, 347
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 156, 345, 417
BssMI GATC 4 cut(s) 223, 339, 504, 531
BssT1I CCWWGG 3 cut(s) 156, 345, 417
Bst4CI ACNGT 2 cut(s) 16, 195
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 355, 397
BstDSI CCRYGG 2 cut(s) 156, 417
BstF5I GGATG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 4 cut(s) 226, 342, 507, 534
BstMBI GATC 4 cut(s) 223, 339, 504, 531
BstMWI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
BstV1I GCAGC 4 cut(s) 20, 32, 137, 444
BstX2I RGATCY 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 1 cut(s) 416
BsuTUI ATCGAT 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 156, 417
BtsCI GGATG 1 cut(s) 392
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 170
CfoI GCGC 1 cut(s) 489
Cfr13I GGNCC 1 cut(s) 26
ClaI ATCGAT 1 cut(s) 102
CviAII CATG 2 cut(s) 157, 418
CviJI RGCY 7 cut(s) 53, 128, 155, 212, 416, 498, 510
CviKI_1 RGCY 7 cut(s) 53, 128, 155, 212, 416, 498, 510
DdeI CTNAG 2 cut(s) 355, 397
DpnI GATC 4 cut(s) 225, 341, 506, 533
DpnII GATC 4 cut(s) 223, 339, 504, 531
EaeI YGGCCR 1 cut(s) 414
Eco130I CCWWGG 3 cut(s) 156, 345, 417
Eco24I GRGCYC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 38
EcoT14I CCWWGG 3 cut(s) 156, 345, 417
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 3 cut(s) 156, 345, 417
FaeI CATG 2 cut(s) 160, 421
FaiI YATR 7 cut(s) 152, 158, 268, 311, 419, 429, 536
FatI CATG 2 cut(s) 156, 417
FbaI TGATCA 1 cut(s) 531
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
FokI GGATG 1 cut(s) 379
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
FspBI CTAG 2 cut(s) 140, 577
GlaI GCGC 1 cut(s) 488
GluI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
GsaI CCCAGC 1 cut(s) 374
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 489
Hin1II CATG 2 cut(s) 160, 421
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HinfI GANTC 1 cut(s) 204
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 91, 334
Hpy166II GTNNAC 3 cut(s) 237, 274, 307
Hpy188I TCNGA 2 cut(s) 134, 504
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 3 cut(s) 237, 274, 307
Hpy99I CGWCG 2 cut(s) 134, 521
HpyCH4III ACNGT 2 cut(s) 16, 195
HpyCH4IV ACGT 2 cut(s) 233, 239
HpyCH4V TGCA 2 cut(s) 125, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
HpyF3I CTNAG 2 cut(s) 355, 397
HpySE526I ACGT 2 cut(s) 233, 239
Hsp92II CATG 2 cut(s) 160, 421
HspAI GCGC 1 cut(s) 487
Ksp22I TGATCA 1 cut(s) 531
Kzo9I GATC 4 cut(s) 223, 339, 504, 531
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 9 cut(s) 9, 42, 154, 178, 301, 306, 306, 356, 372
Lsp1109I GCAGC 4 cut(s) 20, 32, 137, 444
LweI GCATC 1 cut(s) 401
MaeI CTAG 2 cut(s) 140, 577
MaeII ACGT 2 cut(s) 233, 239
MaeIII GTNAC 1 cut(s) 187
MalI GATC 4 cut(s) 225, 341, 506, 533
MboI GATC 4 cut(s) 223, 339, 504, 531
MboII GAAGA 1 cut(s) 218
MfeI CAATTG 2 cut(s) 261, 445
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 2 cut(s) 93, 500
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 5 cut(s) 214, 256, 261, 445, 467
MluNI TGGCCA 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 4 cut(s) 34, 69, 406, 565
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 3 cut(s) 155, 365, 527
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 1 cut(s) 293
MunI CAATTG 2 cut(s) 261, 445
MwoI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
NcoI CCATGG 2 cut(s) 156, 417
NdeII GATC 4 cut(s) 223, 339, 504, 531
NlaIII CATG 2 cut(s) 160, 421
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 187
PaeR7I CTCGAG 1 cut(s) 38
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 199, 513
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 6 cut(s) 10, 22, 127, 434, 464, 575
PspFI CCCAGC 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 26
PspXI VCTCGAGB 1 cut(s) 38
PsuI RGATCY 1 cut(s) 223
RseI CAYNNNNRTG 3 cut(s) 155, 365, 527
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
Sau3AI GATC 4 cut(s) 223, 339, 504, 531
Sau96I GGNCC 1 cut(s) 26
SduI GDGCHC 2 cut(s) 93, 500
SetI ASST 4 cut(s) 236, 242, 279, 347
SfaNI GCATC 1 cut(s) 401
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 3 cut(s) 155, 365, 527
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 5 cut(s) 214, 256, 261, 445, 467
SsiI CCGC 2 cut(s) 463, 574
SspMI CTAG 2 cut(s) 140, 577
StyI CCWWGG 3 cut(s) 156, 345, 417
TaaI ACNGT 2 cut(s) 16, 195
TaiI ACGT 2 cut(s) 236, 242
TaqI TCGA 3 cut(s) 39, 102, 202
TasI AATT 5 cut(s) 214, 256, 261, 445, 467
TauI GCSGC 2 cut(s) 465, 576
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 4 cut(s) 8, 20, 125, 432
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 17, 97
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 2 cut(s) 140, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.