MD10G1325100.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000301
Physical Location & Seq
Reverse (-)
40578644 .. 40579096
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1325100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGAAAGCCTTCAAGGTTTTGCTTGTTCTTTCCATGGTAATGGCTTTAGCCATTACTCTCTCTGCAACTAAGGACCAAGAAGAATCATTCTTGGATGAGGAAAACAAAGATACAGATGATGCAACCAAAAGCATGCAATCAGATAAAACCACTTCTCTTAGGGGAAGAAGCCGCTTGCTTGCCTCTCGGGCCACTGTGACTACATGCGACAAACACCCTAGGGTTTGTCGGGCATCGGGCAGTGCAGGGCCAGATTGCTGCAAGAAGAAATGTGTGGATACGAACACAGACAGAGTAAACTGTGGCACGTGTGGGAAGAAATGCAAGTACGGGGAGACATGCTGCAAAGGCAAGTGTGTGAACCCTAGGTCTGACAAGAAAAACTGTGGGAGCTGCAACAACAAATGCAAGAAAGGCAGTTCATGTGTCTATGGGATGTGCAGCTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

151

Amino Acids

16.24

Weight (kDa)

9.07

Isoelectric Point (pI)

23.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 67 - 150 1.9e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 172
AcvI CACGTG 1 cut(s) 309
AfaI GTAC 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 1 cut(s) 13
AluBI AGCT 2 cut(s) 393, 444
AluI AGCT 2 cut(s) 393, 444
Alw26I GTCTC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 2 cut(s) 189, 248
ApeKI GCWGC 4 cut(s) 258, 342, 393, 441
Asp700I GAANNNNTTC 1 cut(s) 8
AspA2I CCTAGG 2 cut(s) 218, 365
AspS9I GGNCC 3 cut(s) 73, 189, 248
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 1 cut(s) 73
AvrII CCTAGG 2 cut(s) 218, 365
BbrPI CACGTG 1 cut(s) 309
BbvI GCAGC 3 cut(s) 245, 329, 380
BciVI GTATCC 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 219, 366
BfuI GTATCC 1 cut(s) 271
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 5 cut(s) 172, 259, 343, 394, 442
BlnI CCTAGG 2 cut(s) 218, 365
BlsI GCNGC 5 cut(s) 173, 260, 344, 395, 443
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 73, 189, 248
BmsI GCATC 2 cut(s) 109, 242
BsaAI YACGTR 1 cut(s) 309
BsaJI CCNNGG 3 cut(s) 33, 218, 365
BseDI CCNNGG 3 cut(s) 33, 218, 365
BseGI GGATG 2 cut(s) 100, 441
BseXI GCAGC 3 cut(s) 245, 329, 380
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 2 cut(s) 191, 250
BsiHKCI CYCGRG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 2 cut(s) 191, 250
BsoBI CYCGRG 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 172
BspANI GGCC 2 cut(s) 191, 250
BssECI CCNNGG 3 cut(s) 33, 218, 365
BssT1I CCWWGG 3 cut(s) 33, 218, 365
Bst4CI ACNGT 3 cut(s) 196, 302, 386
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 134, 176, 180
BstDEI CTNAG 2 cut(s) 69, 158
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 2 cut(s) 100, 441
BstMAI GTCTC 1 cut(s) 329
BstMWI GCNNNNNNNGC 3 cut(s) 188, 348, 414
BstNSI RCATGY 3 cut(s) 136, 207, 342
BstV1I GCAGC 3 cut(s) 245, 329, 380
BstXI CCANNNNNNTGG 1 cut(s) 40
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 2 cut(s) 191, 250
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 100, 441
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 192, 247
Cac8I GCNNGC 3 cut(s) 134, 176, 180
Cfr13I GGNCC 3 cut(s) 73, 189, 248
Csp6I GTAC 1 cut(s) 328
CviAII CATG 6 cut(s) 34, 133, 204, 339, 423, 450
CviJI RGCY 8 cut(s) 8, 44, 50, 171, 191, 250, 393, 444
CviKI_1 RGCY 8 cut(s) 8, 44, 50, 171, 191, 250, 393, 444
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 69, 158
Eco130I CCWWGG 3 cut(s) 33, 218, 365
Eco47I GGWCC 1 cut(s) 73
Eco72I CACGTG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 186
EcoT14I CCWWGG 3 cut(s) 33, 218, 365
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 3 cut(s) 33, 218, 365
FaeI CATG 6 cut(s) 37, 136, 207, 342, 426, 453
FaiI YATR 8 cut(s) 35, 134, 205, 340, 424, 432, 447, 451
FatI CATG 6 cut(s) 33, 132, 203, 338, 422, 449
Fnu4HI GCNGC 5 cut(s) 172, 259, 343, 394, 442
FokI GGATG 2 cut(s) 107, 448
Fsp4HI GCNGC 5 cut(s) 172, 259, 343, 394, 442
FspBI CTAG 2 cut(s) 219, 366
GluI GCNGC 5 cut(s) 172, 259, 343, 394, 442
HaeIII GGCC 2 cut(s) 191, 250
Hin1II CATG 6 cut(s) 37, 136, 207, 342, 426, 453
HinfI GANTC 1 cut(s) 83
Hpy166II GTNNAC 2 cut(s) 298, 361
Hpy188I TCNGA 2 cut(s) 142, 373
Hpy8I GTNNAC 2 cut(s) 298, 361
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 3 cut(s) 196, 302, 386
HpyCH4IV ACGT 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 348, 414
HpyF3I CTNAG 2 cut(s) 69, 158
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 6 cut(s) 37, 136, 207, 342, 426, 453
LmnI GCTCC 1 cut(s) 390
LpnPI CCDG 2 cut(s) 231, 264
Lsp1109I GCAGC 3 cut(s) 245, 329, 380
LweI GCATC 2 cut(s) 109, 242
MaeI CTAG 2 cut(s) 219, 366
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 4 cut(s) 92, 177, 277, 328
MnlI CCTC 2 cut(s) 91, 193
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 188, 348, 414
NcoI CCATGG 1 cut(s) 33
NlaIII CATG 6 cut(s) 37, 136, 207, 342, 426, 453
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 3 cut(s) 136, 207, 342
PaeI GCATGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 5 cut(s) 173, 260, 344, 395, 443
PmaCI CACGTG 1 cut(s) 309
PmlI CACGTG 1 cut(s) 309
Ppu21I YACGTR 1 cut(s) 309
PspCI CACGTG 1 cut(s) 309
PspPI GGNCC 3 cut(s) 73, 189, 248
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 38
SatI GCNGC 5 cut(s) 172, 259, 343, 394, 442
Sau96I GGNCC 3 cut(s) 73, 189, 248
SetI ASST 5 cut(s) 18, 311, 371, 395, 446
SfaNI GCATC 2 cut(s) 109, 242
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 38
SphI GCATGC 1 cut(s) 136
SsiI CCGC 1 cut(s) 172
SspMI CTAG 2 cut(s) 219, 366
StyI CCWWGG 3 cut(s) 33, 218, 365
TaaI ACNGT 3 cut(s) 196, 302, 386
TaiI ACGT 1 cut(s) 311
TauI GCSGC 1 cut(s) 174
TfiI GAWTC 1 cut(s) 83
TscAI CASTG 2 cut(s) 199, 247
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 4 cut(s) 258, 342, 393, 441
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 17, 411
TspRI CASTG 2 cut(s) 199, 247
VpaK11BI GGWCC 1 cut(s) 73
XceI RCATGY 3 cut(s) 136, 207, 342
XmaJI CCTAGG 2 cut(s) 218, 365
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 219, 366
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.