MD10G1325000.v1.1

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000301
Physical Location & Seq
Reverse (-)
40573607 .. 40574056
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1325000.v1.1.491

Sequence Viewer

Length: 450 bp
ATGAAATCCCTCAAGGTTTTGTTTCAGCTAGCGATGCTGATGGCTTTAGTGGCCATTACTCTCTCGGCAACACCAGACCAAGAATTCGAAGAATCATTCTTCGGCGAGGAAGACAACAGTATTAATGTTTCAAACAGTGAAACCTTTAAACAAGAAAAGACTTCTCTAAGAGGCATAAGCCGATTCCTTGCTTCTCGGGGTCGGGCAGTGACTTGCGACAGTAACCCTAAGGTTTGTCGAGGAGGGACTCATTGTTGCCACAAGAAATGTGTGGATTTGAAGACGGACAATCTCCACTGTGGCCAGTGTGGGGTGAAAAGCCATCACTCACAGATATGCTGCCACGGTCATCTTGTGAACCCAATTTCTGATAAGAAACACTGTGGCAGCTGTGACAACCATTGCAAAAAAGGCAGTTCATGTGCGCTTGGGATGTGCAGCTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

150

Amino Acids

16.26

Weight (kDa)

8.39

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 70 - 149 5.4e-21 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 51, 301
AcsI RAATTY 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 2 cut(s) 132, 280
AluBI AGCT 3 cut(s) 28, 390, 441
AluI AGCT 3 cut(s) 28, 390, 441
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 2 cut(s) 51, 301
ApeKI GCWGC 3 cut(s) 339, 387, 438
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AseI ATTAAT 1 cut(s) 123
AspLEI GCGC 1 cut(s) 427
AsuHPI GGTGA 1 cut(s) 325
AsuII TTCGAA 1 cut(s) 87
AsuNHI GCTAGC 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 195
AxyI CCTNAGG 1 cut(s) 228
BalI TGGCCA 2 cut(s) 53, 303
BbsI GAAGAC 2 cut(s) 117, 287
BbvI GCAGC 2 cut(s) 326, 399
BccI CCATC 2 cut(s) 34, 330
BfaI CTAG 1 cut(s) 29
BisI GCNGC 3 cut(s) 340, 388, 439
BlsI GCNGC 3 cut(s) 341, 389, 440
BmeT110I CYCGRG 1 cut(s) 195
BmsI GCATC 1 cut(s) 24
BmtI GCTAGC 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 117, 287
Bpu14I TTCGAA 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 343
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMI GCAATG 1 cut(s) 400
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 326, 399
BshFI GGCC 2 cut(s) 53, 303
BsiHKCI CYCGRG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 2 cut(s) 53, 303
BsoBI CYCGRG 1 cut(s) 195
Bsp119I TTCGAA 1 cut(s) 87
BspANI GGCC 2 cut(s) 53, 303
BspOI GCTAGC 1 cut(s) 32
BspT104I TTCGAA 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 343
Bst4CI ACNGT 6 cut(s) 119, 137, 221, 299, 347, 383
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 3 cut(s) 167, 228, 447
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 427
BstMWI GCNNNNNNNGC 3 cut(s) 34, 50, 411
BstV1I GCAGC 2 cut(s) 326, 399
BstV2I GAAGAC 2 cut(s) 117, 287
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 2 cut(s) 53, 303
BtgI CCRYGG 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 438
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 5 cut(s) 142, 213, 295, 311, 379
Cac8I GCNNGC 1 cut(s) 30
CfoI GCGC 1 cut(s) 427
CviAII CATG 1 cut(s) 420
CviJI RGCY 8 cut(s) 28, 44, 53, 180, 303, 321, 390, 441
CviKI_1 RGCY 8 cut(s) 28, 44, 53, 180, 303, 321, 390, 441
DdeI CTNAG 3 cut(s) 167, 228, 447
DraI TTTAAA 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 51, 301
Eco81I CCTNAGG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 83
FaeI CATG 1 cut(s) 423
FaiI YATR 4 cut(s) 176, 337, 421, 444
FaqI GGGAC 1 cut(s) 259
FatI CATG 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 340, 388, 439
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 3 cut(s) 340, 388, 439
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 426
GluI GCNGC 3 cut(s) 340, 388, 439
HaeIII GGCC 2 cut(s) 53, 303
HhaI GCGC 1 cut(s) 427
Hin1II CATG 1 cut(s) 423
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 3 cut(s) 92, 183, 247
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 358
HpyCH4III ACNGT 6 cut(s) 119, 137, 221, 299, 347, 383
HpyCH4V TGCA 2 cut(s) 405, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 50, 411
HpyF3I CTNAG 3 cut(s) 167, 228, 447
Hsp92II CATG 1 cut(s) 423
HspAI GCGC 1 cut(s) 425
LpnPI CCDG 2 cut(s) 87, 317
Lsp1109I GCAGC 2 cut(s) 326, 399
LweI GCATC 1 cut(s) 24
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 3 cut(s) 208, 221, 392
MboII GAAGA 4 cut(s) 91, 101, 122, 292
MlsI TGGCCA 2 cut(s) 53, 303
MluCI AATT 2 cut(s) 83, 363
MluNI TGGCCA 2 cut(s) 53, 303
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 5 cut(s) 20, 100, 164, 233, 236
Mox20I TGGCCA 2 cut(s) 53, 303
MscI TGGCCA 2 cut(s) 53, 303
MseI TTAA 2 cut(s) 123, 147
MslI CAYNNNNRTG 1 cut(s) 334
Msp20I TGGCCA 2 cut(s) 53, 303
MspA1I CMGCKG 1 cut(s) 390
MwoI GCNNNNNNNGC 3 cut(s) 34, 50, 411
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 1 cut(s) 423
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 2 cut(s) 208, 392
NspV TTCGAA 1 cut(s) 87
PfeI GAWTC 2 cut(s) 92, 183
PkrI GCNGC 3 cut(s) 341, 389, 440
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 123
PvuII CAGCTG 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 2 cut(s) 123, 147
SatI GCNGC 3 cut(s) 340, 388, 439
SchI GAGTC 1 cut(s) 241
SetI ASST 6 cut(s) 18, 30, 146, 234, 392, 443
SfaNI GCATC 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 2 cut(s) 83, 363
SspMI CTAG 1 cut(s) 29
TaaI ACNGT 6 cut(s) 119, 137, 221, 299, 347, 383
TaqI TCGA 2 cut(s) 87, 238
TasI AATT 2 cut(s) 83, 363
TfiI GAWTC 2 cut(s) 92, 183
Tru1I TTAA 2 cut(s) 123, 147
Tru9I TTAA 2 cut(s) 123, 147
TscAI CASTG 5 cut(s) 142, 213, 302, 311, 386
TseFI GTSAC 2 cut(s) 208, 392
TseI GCWGC 3 cut(s) 339, 387, 438
Tsp45I GTSAC 2 cut(s) 208, 392
TspDTI ATGAA 2 cut(s) 17, 408
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 5 cut(s) 142, 213, 302, 311, 386
VspI ATTAAT 1 cut(s) 123
XapI RAATTY 1 cut(s) 83
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.