Rorug05G0116800

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000301
Physical Location & Seq
Forward (+)
10338377 .. 10342083
3707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0116800.1

Sequence Viewer

Length: 1785 bp
ATGGCCGAATCCGGCCGATGCCGAAGGCGAAACTGGACGGAACTCCCCGACGAGTTAACGGCGGCGATTCTTTCACGGCTGGGAGCGGTGGAGATCCTGGAGAGCGCTCAGAAAGTTTGCATGAAGTGGCGCAAAATATCCAAGGAGCCTCTCTTCTGGCGCAAAATCGACATGCGCAACGATGGTGATCTTGATGACTGTGACTACGAGTCCATGTTCAGCCACATCGTTGATCGGAGCTCCGGTAATGTCGTCGATATCAATATCGAGCACTTCGGCACCGACCAACTACTCACCTTTATCACCAGAAGTGCGAGTACAGTCAGGCGCATCCGACTATTTTCTTGCGATGAGGTGTCAGATGAGGGATTGAGTAGAATGGCTTCGAAACTGCGGTTGTTGGAGGAACTTGACATTTCATTGTGCGCTGGTATCTCACATGAAGCTGTAAAAGCAGTTGGGCGCTCTTGCCCTCTTTTGAAGTCATTCAAATTCAACAAGGAGTGGTGCAGATTCTCTGATGATGAGTCTGACTCTGATGAGTATGGGTCTGACTTTGATGTGCATGACTTTGATGATAGTGATGACGATTATGCACATGCTCCTTTTGCTCGTGCTCCTGTTGTTCATGCTCCTGTTGCTCATGCTCGTGTTAGAAAAGATGACAATGTAGATGCACTTGCTATAGCAGGAACGATGCAAGATTTACACCACCTCCAGCTTTATGGGAATAAGCTTACAAATGATGGCTTGAGAAAAATTCTTGATTGTTGTCCTCATCTTGAGTCACTTGATCTGCGCCTTTGCTTCAATCTCAATATGGGGATAGATTTAGAAACAAGATGTGCTGAACGGATTAAAAGGTTGTGGCTTCCCCATGATTCCATAGAGGGAAAAGAATTTACCGCTAGATCAGATGGGTACTGCTTTAAATGGGTTGAACTCCCAGATAACATTACTATGTCGATACTGTCACGGCTTGGAACAATTGATATCCTGGAGAGTGCTCAGAAGGTATGCATGAAGTGGCGCAAAATCTGCAAGGAACTGAAGTGGAACACCATAGACATGCGCAATGATAGTGCTCCTGACATTACCTTCGACTTAGAGAAGATGTGCCGCCATGTTGTGGATCTTAGTTGTGGTAATTTGGTTGATGTGAATGTTGAGGACTTTATCTCGGATGAGCTCCTAGAGTATATCACTGATAGTTCGAGTGGAATCAGACGCCTGCGACTTGTGTGTTCTTATTACATATCAGATGAGGGATTGATTGAAGTTGCATCAAAACTTCCGCTGTTAGAGGATCTTGACATCTCATTATGCGGAAATCTGTCTCATAAACCTGTGAAAGTGCTTGGGCACTCTTGCCGTCTTTTGAAATCATTCAAATTGAATAAAGAATGGCACAAATATTCAGACAACTTCTTTGAAGAGATTATTGATGATGAAGGTCCTTGGAGTAAAGATGACGATGCACTTGCTATTGCAGGAACGATGCATGGTTTATACCACCTGCAGCTTTTTGGGAATCAGCTGACCAATGATGGCTTAAAGGCAATTCTTGATGGTTGTCCTCATCTTGAGTCACTAGATCTTCGCTATTGTTTCAACCTTAATCTGGAGGGAGATTTGGGAAGAAGATGCACTGAACAAATTAAAAGATTGTTGCTTCCCAAAGATTCAATTGCTGGATATGGATTTCTGCCTTCTACCGATTGGCAAGCTCCCAGATCACCCAAATATAATGGAGGTTGGGGCTGGGACCCTTTTGATGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

594

Amino Acids

67.81

Weight (kDa)

5.07

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 12 - 58 1.9e-08 F-box-like
F-box-like PF12937 312 - 357 9.3e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1524
Acc16I TGCGCA 2 cut(s) 176, 1073
Acc36I ACCTGC 1 cut(s) 1524
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 1129
AccBSI CCGCTC 1 cut(s) 86
AciI CCGC 7 cut(s) 62, 86, 394, 906, 1120, 1295, 1326
AclWI GGATC 3 cut(s) 88, 1140, 1314
AcoI YGGCCR 2 cut(s) 3, 13
AcsI RAATTY 3 cut(s) 491, 759, 899
AcuI CTGAAG 1 cut(s) 1070
AcyI GRCGYC 1 cut(s) 1228
AfaI GTAC 2 cut(s) 319, 923
AfeI AGCGCT 1 cut(s) 106
AfiI CCNNNNNNNGG 2 cut(s) 1129, 1621
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 96, 996
AluBI AGCT 8 cut(s) 240, 446, 721, 736, 1189, 1522, 1537, 1727
AluI AGCT 8 cut(s) 240, 446, 721, 736, 1189, 1522, 1537, 1727
Alw21I GWGCWC 6 cut(s) 242, 273, 619, 1009, 1087, 1191
Alw26I GTCTC 1 cut(s) 1341
AlwI GGATC 3 cut(s) 88, 1140, 1314
Aor51HI AGCGCT 1 cut(s) 106
AoxI GGCC 2 cut(s) 3, 13
ApeKI GCWGC 1 cut(s) 1519
ApoI RAATTY 3 cut(s) 491, 759, 899
ArsI GACNNNNNNTTYG 4 cut(s) 1082, 1114, 1412, 1444
Asp700I GAANNNNTTC 3 cut(s) 382, 485, 1385
AspS9I GGNCC 2 cut(s) 1454, 1765
AsuHPI GGTGA 4 cut(s) 197, 286, 295, 1728
AsuII TTCGAA 1 cut(s) 386
AvaII GGWCC 2 cut(s) 1454, 1765
BaeGI GKGCMC 1 cut(s) 1365
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 242, 1191
BarI GAAGNNNNNNTAC 4 cut(s) 301, 333, 367, 399
BauI CACGAG 2 cut(s) 612, 648
Bbv12I GWGCWC 6 cut(s) 242, 273, 619, 1009, 1087, 1191
BbvI GCAGC 1 cut(s) 1531
BccI CCATC 5 cut(s) 176, 740, 911, 1541, 1562
BceAI ACGGC 4 cut(s) 75, 92, 992, 1356
BcgI CGANNNNNNTGC 2 cut(s) 1463, 1497
BciT130I CCWGG 2 cut(s) 98, 998
BcoDI GTCTC 1 cut(s) 1341
BfaI CTAG 3 cut(s) 909, 1193, 1592
BfmI CTRYAG 2 cut(s) 684, 1517
BfoI RGCGCY 2 cut(s) 108, 466
BfuAI ACCTGC 1 cut(s) 1524
BglII AGATCT 1 cut(s) 1594
BisI GCNGC 3 cut(s) 63, 1120, 1520
BlsI GCNGC 3 cut(s) 64, 1121, 1521
Bme1390I CCNGG 2 cut(s) 98, 998
Bme18I GGWCC 2 cut(s) 1454, 1765
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 1454, 1765
BmiI GGNNCC 4 cut(s) 147, 280, 1766, 1767
BmrFI CCNGG 2 cut(s) 98, 998
BmsI GCATC 8 cut(s) 8, 339, 664, 687, 1292, 1465, 1488, 1634
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 4 cut(s) 119, 701, 1019, 1643
Bpu14I TTCGAA 1 cut(s) 386
BpuEI CTTGAG 3 cut(s) 772, 803, 1604
BsaBI GATNNNNATC 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 1228
BsaJI CCNNGG 2 cut(s) 141, 1457
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 4 cut(s) 229, 259, 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 1129, 1621
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 1081
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 2 cut(s) 98, 998
BseDI CCNNGG 2 cut(s) 141, 1457
BseGI GGATG 2 cut(s) 330, 1189
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 1129, 1621
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 2 cut(s) 122, 1022
BseNI ACTGG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 1365
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 1531
BseYI CCCAGC 2 cut(s) 79, 1761
BsgI GTGCAG 1 cut(s) 529
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 2 cut(s) 5, 15
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 6 cut(s) 242, 273, 619, 1009, 1087, 1191
BsiSI CCGG 2 cut(s) 12, 243
BslFI GGGAC 1 cut(s) 1778
BslI CCNNNNNNNGG 2 cut(s) 1129, 1621
BsmAI GTCTC 1 cut(s) 1341
BsmFI GGGAC 1 cut(s) 1778
BsnI GGCC 2 cut(s) 5, 15
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 7 cut(s) 242, 273, 619, 1009, 1087, 1191, 1365
Bsp143I GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
BspACI CCGC 7 cut(s) 62, 86, 394, 906, 1120, 1295, 1326
BspANI GGCC 2 cut(s) 5, 15
BspCNI CTCAG 2 cut(s) 121, 1021
BspLI GGNNCC 4 cut(s) 147, 280, 1766, 1767
BspMAI CTGCAG 1 cut(s) 1521
BspMI ACCTGC 1 cut(s) 1524
BspPI GGATC 3 cut(s) 88, 1140, 1314
BspT104I TTCGAA 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 1081
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 141, 1457
BssMI GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
BssNI GRCGYC 1 cut(s) 1228
BssSI CACGAG 2 cut(s) 612, 648
BssT1I CCWWGG 2 cut(s) 141, 1457
Bst2BI CACGAG 2 cut(s) 612, 648
Bst2UI CCWGG 2 cut(s) 98, 998
Bst4CI ACNGT 3 cut(s) 200, 322, 972
Bst6I CTCTTC 2 cut(s) 158, 1428
BstACI GRCGYC 1 cut(s) 1228
BstAPI GCANNNNNTGC 1 cut(s) 1038
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 1232, 1725
BstDEI CTNAG 4 cut(s) 108, 1008, 1105, 1136
BstF5I GGATG 2 cut(s) 330, 1189
BstH2I RGCGCY 2 cut(s) 108, 466
BstKTI GATC 9 cut(s) 96, 190, 235, 796, 914, 1135, 1309, 1597, 1736
BstMAI GTCTC 1 cut(s) 1341
BstMBI GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 4 cut(s) 452, 608, 638, 1038
BstNI CCWGG 2 cut(s) 98, 998
BstNSI RCATGY 3 cut(s) 175, 602, 1072
BstSCI CCNGG 2 cut(s) 96, 996
BstSFI CTRYAG 2 cut(s) 684, 1517
BstSLI GKGCMC 1 cut(s) 1365
BstV1I GCAGC 1 cut(s) 1531
BstX2I RGATCY 4 cut(s) 93, 1132, 1306, 1594
BstXI CCANNNNNNTGG 1 cut(s) 725
BstYI RGATCY 4 cut(s) 93, 1132, 1306, 1594
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 2 cut(s) 5, 15
BtgZI GCGATG 1 cut(s) 363
BtsCI GGATG 2 cut(s) 330, 1189
BtsIMutI CAGTG 2 cut(s) 1203, 1647
BveI ACCTGC 1 cut(s) 1524
Cac8I GCNNGC 2 cut(s) 1232, 1725
Cfr13I GGNCC 2 cut(s) 1454, 1765
CseI GACGC 1 cut(s) 1236
Csp6I GTAC 2 cut(s) 318, 922
CviQI GTAC 2 cut(s) 318, 922
DdeI CTNAG 4 cut(s) 108, 1008, 1105, 1136
DpnI GATC 9 cut(s) 95, 189, 234, 795, 913, 1134, 1308, 1596, 1735
DpnII GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
DraI TTTAAA 1 cut(s) 931
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 3, 13
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 158, 1428
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 2 cut(s) 158, 1428
Ecl136II GAGCTC 2 cut(s) 240, 1189
EclXI CGGCCG 1 cut(s) 13
Eco130I CCWWGG 2 cut(s) 141, 1457
Eco24I GRGCYC 2 cut(s) 242, 1191
Eco32I GATATC 2 cut(s) 259, 994
Eco47I GGWCC 2 cut(s) 1454, 1765
Eco47III AGCGCT 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 13
Eco53kI GAGCTC 2 cut(s) 240, 1189
Eco57I CTGAAG 1 cut(s) 1070
EcoICRI GAGCTC 2 cut(s) 240, 1189
EcoO109I RGGNCCY 2 cut(s) 1454, 1765
EcoRII CCWGG 2 cut(s) 96, 996
EcoRV GATATC 2 cut(s) 259, 994
EcoT14I CCWWGG 2 cut(s) 141, 1457
EcoT22I ATGCAT 2 cut(s) 1022, 1503
EcoT38I GRGCYC 2 cut(s) 242, 1191
ErhI CCWWGG 2 cut(s) 141, 1457
FaqI GGGAC 1 cut(s) 1778
Fnu4HI GCNGC 3 cut(s) 63, 1120, 1520
FokI GGATG 2 cut(s) 317, 1196
FriOI GRGCYC 2 cut(s) 242, 1191
Fsp4HI GCNGC 3 cut(s) 63, 1120, 1520
FspBI CTAG 3 cut(s) 909, 1193, 1592
FspI TGCGCA 2 cut(s) 176, 1073
GluI GCNGC 3 cut(s) 63, 1120, 1520
GsaI CCCAGC 2 cut(s) 83, 1765
GsuI CTGGAG 4 cut(s) 119, 701, 1019, 1643
HaeII RGCGCY 2 cut(s) 108, 466
HaeIII GGCC 2 cut(s) 5, 15
HapII CCGG 2 cut(s) 12, 243
HgaI GACGC 1 cut(s) 1236
Hin1I GRCGYC 1 cut(s) 1228
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 734
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 2 cut(s) 12, 243
HphI GGTGA 4 cut(s) 197, 286, 295, 1728
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 8 cut(s) 191, 764, 782, 1088, 1310, 1565, 1583, 1622
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 2 cut(s) 53, 257
HpyAV CCTTC 5 cut(s) 18, 1006, 1108, 1445, 1719
HpyCH4III ACNGT 3 cut(s) 200, 322, 972
HpyF10VI GCNNNNNNNGC 4 cut(s) 452, 608, 638, 1038
HpyF3I CTNAG 4 cut(s) 108, 1008, 1105, 1136
Hsp92I GRCGYC 1 cut(s) 1228
KflI GGGWCCC 1 cut(s) 1765
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
Lsp1109I GCAGC 1 cut(s) 1531
LweI GCATC 8 cut(s) 8, 339, 664, 687, 1292, 1465, 1488, 1634
MaeI CTAG 3 cut(s) 909, 1193, 1592
MaeIII GTNAC 4 cut(s) 200, 786, 972, 1587
MalI GATC 9 cut(s) 95, 189, 234, 795, 913, 1134, 1308, 1596, 1735
MbiI CCGCTC 1 cut(s) 86
MboI GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
MboII GAAGA 6 cut(s) 145, 1123, 1445, 1589, 1650, 1653
MfeI CAATTG 2 cut(s) 987, 1686
MflI RGATCY 4 cut(s) 93, 1132, 1306, 1594
MhlI GDGCHC 7 cut(s) 242, 273, 619, 1009, 1087, 1191, 1365
MluCI AATT 9 cut(s) 491, 759, 899, 987, 1147, 1391, 1560, 1656, 1686
MlyI GAGTC 5 cut(s) 218, 527, 536, 794, 1595
MmeI TCCRAC 2 cut(s) 358, 381
Mph1103I ATGCAT 2 cut(s) 1022, 1503
MroXI GAANNNNTTC 3 cut(s) 382, 485, 1385
MseI TTAA 6 cut(s) 56, 858, 930, 1553, 1617, 1659
MslI CAYNNNNRTG 3 cut(s) 648, 959, 1067
MspA1I CMGCKG 2 cut(s) 1297, 1537
MspI CCGG 2 cut(s) 12, 243
MspR9I CCNGG 2 cut(s) 98, 998
MunI CAATTG 2 cut(s) 987, 1686
MvaI CCWGG 2 cut(s) 98, 998
MwoI GCNNNNNNNGC 4 cut(s) 452, 608, 638, 1038
NdeII GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
NlaIV GGNNCC 4 cut(s) 147, 280, 1766, 1767
NmuCI GTSAC 4 cut(s) 200, 786, 972, 1587
NsbI TGCGCA 2 cut(s) 176, 1073
NsiI ATGCAT 2 cut(s) 1022, 1503
NspI RCATGY 3 cut(s) 175, 602, 1072
NspV TTCGAA 1 cut(s) 386
PaqCI CACCTGC 1 cut(s) 1524
PdmI GAANNNNTTC 3 cut(s) 382, 485, 1385
PfeI GAWTC 7 cut(s) 8, 67, 513, 881, 1221, 1531, 1682
PflMI CCANNNNNTGG 1 cut(s) 1129
PfoI TCCNGGA 2 cut(s) 96, 996
PkrI GCNGC 3 cut(s) 64, 1121, 1521
PleI GAGTC 5 cut(s) 217, 527, 535, 793, 1594
PpsI GAGTC 5 cut(s) 217, 527, 535, 793, 1594
PpuMI RGGWCCY 2 cut(s) 1454, 1765
Psp124BI GAGCTC 2 cut(s) 242, 1191
Psp5II RGGWCCY 2 cut(s) 1454, 1765
Psp6I CCWGG 2 cut(s) 96, 996
PspFI CCCAGC 2 cut(s) 79, 1761
PspGI CCWGG 2 cut(s) 96, 996
PspN4I GGNNCC 4 cut(s) 147, 280, 1766, 1767
PspPI GGNCC 2 cut(s) 1454, 1765
PspPPI RGGWCCY 2 cut(s) 1454, 1765
PstI CTGCAG 1 cut(s) 1521
PsuI RGATCY 4 cut(s) 93, 1132, 1306, 1594
PvuII CAGCTG 1 cut(s) 1537
RsaI GTAC 2 cut(s) 319, 923
RsaNI GTAC 2 cut(s) 318, 922
RseI CAYNNNNRTG 3 cut(s) 648, 959, 1067
SacI GAGCTC 2 cut(s) 242, 1191
SaqAI TTAA 6 cut(s) 56, 858, 930, 1553, 1617, 1659
SatI GCNGC 3 cut(s) 63, 1120, 1520
Sau3AI GATC 9 cut(s) 93, 187, 232, 793, 911, 1132, 1306, 1594, 1733
Sau96I GGNCC 2 cut(s) 1454, 1765
SchI GAGTC 5 cut(s) 218, 527, 536, 794, 1595
ScrFI CCNGG 2 cut(s) 98, 998
SduI GDGCHC 7 cut(s) 242, 273, 619, 1009, 1087, 1191, 1365
SfaNI GCATC 8 cut(s) 8, 339, 664, 687, 1292, 1465, 1488, 1634
SfcI CTRYAG 2 cut(s) 684, 1517
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 2 cut(s) 1454, 1765
SmiMI CAYNNNNRTG 3 cut(s) 648, 959, 1067
SmlI CTYRAG 3 cut(s) 751, 782, 1583
SmoI CTYRAG 3 cut(s) 751, 782, 1583
Sse9I AATT 9 cut(s) 491, 759, 899, 987, 1147, 1391, 1560, 1656, 1686
SsiI CCGC 7 cut(s) 62, 86, 394, 906, 1120, 1295, 1326
SspI AATATT 1 cut(s) 1415
SspMI CTAG 3 cut(s) 909, 1193, 1592
SstI GAGCTC 2 cut(s) 242, 1191
StyD4I CCNGG 2 cut(s) 96, 996
StyI CCWWGG 2 cut(s) 141, 1457
TaaI ACNGT 3 cut(s) 200, 322, 972
TaqI TCGA 7 cut(s) 168, 255, 267, 386, 965, 1101, 1214
TasI AATT 9 cut(s) 491, 759, 899, 987, 1147, 1391, 1560, 1656, 1686
TatI WGTACW 1 cut(s) 317
TauI GCSGC 2 cut(s) 65, 1122
TfiI GAWTC 7 cut(s) 8, 67, 513, 881, 1221, 1531, 1682
Tru1I TTAA 6 cut(s) 56, 858, 930, 1553, 1617, 1659
Tru9I TTAA 6 cut(s) 56, 858, 930, 1553, 1617, 1659
TscAI CASTG 2 cut(s) 1210, 1654
TseFI GTSAC 4 cut(s) 200, 786, 972, 1587
TseI GCWGC 1 cut(s) 1519
Tsp45I GTSAC 4 cut(s) 200, 786, 972, 1587
TspDTI ATGAA 6 cut(s) 137, 408, 456, 617, 1037, 1464
TspGWI ACGGA 2 cut(s) 53, 868
TspRI CASTG 2 cut(s) 1210, 1654
Van91I CCANNNNNTGG 1 cut(s) 1129
VpaK11BI GGWCC 2 cut(s) 1454, 1765
XapI RAATTY 3 cut(s) 491, 759, 899
XceI RCATGY 3 cut(s) 175, 602, 1072
XmnI GAANNNNTTC 3 cut(s) 382, 485, 1385
XspI CTAG 3 cut(s) 909, 1193, 1592
Zsp2I ATGCAT 2 cut(s) 1022, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.