Rw4G026160

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000301
Physical Location & Seq
Reverse (-)
52344977 .. 52345444
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026160.1

Sequence Viewer

Length: 468 bp
ATGAAATCCCTCAAGGTTTTCTTATTAGTGGTTGCGATGTTGATGGCTTTTGCCGTTACTCTCTCTGCAACACTAGTCGAAGAAGAACTATTTTCCAACAAGGAAACCAAGGCAGCAAATGATCACGAAACCTTTGATGCCAAACGCCAAGGGAAAACCTCTTTGAGGGGAACAAGCAGCGTCTTCCTTGGTTCTAGGGAAGTGACGACATCATCATGCGACAAAAACCCCAAGGTTTGTAGAGCCGCCGCAAACAGCCCAAAATCACATTGCTGCCACAACAAGTGTGTGGACGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTGAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGTGCACACAATGTCCGACATGAAACACTGCGGGAGCTGCGACAACCATTGCAAGAGAGGTGGTTCTTGTGCGTTTGGGATGTGCAACTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

155

Amino Acids

16.8

Weight (kDa)

8.82

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 71 - 155 2.5e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 246, 249, 318, 402
AfiI CCNNNNNNNGG 1 cut(s) 165
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 295
AluBI AGCT 1 cut(s) 408
AluI AGCT 1 cut(s) 408
Alw21I GWGCWC 1 cut(s) 378
Alw44I GTGCAC 1 cut(s) 374
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 5 cut(s) 113, 177, 273, 357, 408
AsuHPI GGTGA 1 cut(s) 343
BaeGI GKGCMC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 175, 305
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 5 cut(s) 125, 189, 260, 344, 395
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 38
BclI TGATCA 1 cut(s) 121
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 2 cut(s) 74, 195
BisI GCNGC 7 cut(s) 114, 178, 246, 249, 274, 358, 409
BlsI GCNGC 7 cut(s) 115, 179, 247, 250, 275, 359, 410
BmgBI CACGTC 1 cut(s) 295
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 2 cut(s) 175, 305
BsaJI CCNNGG 4 cut(s) 108, 148, 187, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse3DI GCAATG 2 cut(s) 268, 418
BseDI CCNNGG 4 cut(s) 108, 148, 187, 231
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMI GCAATG 2 cut(s) 268, 418
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 5 cut(s) 125, 189, 260, 344, 395
BsiHKAI GWGCWC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 4 cut(s) 246, 249, 318, 402
BsrDI GCAATG 2 cut(s) 268, 418
BssECI CCNNGG 4 cut(s) 108, 148, 187, 231
BssMI GATC 1 cut(s) 121
BssT1I CCWWGG 4 cut(s) 108, 148, 187, 231
Bst4CI ACNGT 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 341, 345
BstDEI CTNAG 1 cut(s) 465
BstENI CCTNNNNNAGG 1 cut(s) 163
BstF5I GGATG 1 cut(s) 456
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 5 cut(s) 125, 189, 260, 344, 395
BstV2I GAAGAC 2 cut(s) 175, 305
BtgZI GCGATG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 1 cut(s) 456
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 341, 345
CseI GACGC 1 cut(s) 169
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 3 cut(s) 216, 368, 391
CviJI RGCY 4 cut(s) 47, 245, 258, 408
CviKI_1 RGCY 4 cut(s) 47, 245, 258, 408
DdeI CTNAG 1 cut(s) 465
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eco130I CCWWGG 4 cut(s) 108, 148, 187, 231
EcoNI CCTNNNNNAGG 1 cut(s) 163
EcoT14I CCWWGG 4 cut(s) 108, 148, 187, 231
ErhI CCWWGG 4 cut(s) 108, 148, 187, 231
FaeI CATG 3 cut(s) 219, 371, 394
FaiI YATR 5 cut(s) 217, 355, 369, 392, 462
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 3 cut(s) 215, 367, 390
FauI CCCGC 2 cut(s) 311, 395
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 7 cut(s) 114, 178, 246, 249, 274, 358, 409
FokI GGATG 1 cut(s) 463
Fsp4HI GCNGC 7 cut(s) 114, 178, 246, 249, 274, 358, 409
FspBI CTAG 2 cut(s) 74, 195
GluI GCNGC 7 cut(s) 114, 178, 246, 249, 274, 358, 409
HgaI GACGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 219, 371, 394
HinfI GANTC 1 cut(s) 309
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 292, 376
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 1 cut(s) 125
Hpy8I GTNNAC 2 cut(s) 292, 376
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 6 cut(s) 68, 339, 360, 376, 423, 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 408
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 3 cut(s) 219, 371, 394
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 5 cut(s) 125, 189, 260, 344, 395
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 74, 195
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 55, 202
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 4 cut(s) 92, 95, 175, 310
MhlI GDGCHC 1 cut(s) 378
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 2 cut(s) 120, 411
MnlI CCTC 4 cut(s) 20, 159, 169, 422
MslI CAYNNNNRTG 1 cut(s) 214
MwoI GCNNNNNNNGC 1 cut(s) 408
NdeII GATC 1 cut(s) 121
NlaIII CATG 3 cut(s) 219, 371, 394
NmuCI GTSAC 1 cut(s) 202
PkrI GCNGC 7 cut(s) 115, 179, 247, 250, 275, 359, 410
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 214
SatI GCNGC 7 cut(s) 114, 178, 246, 249, 274, 358, 409
Sau3AI GATC 1 cut(s) 121
SchI GAGTC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 378
SetI ASST 7 cut(s) 18, 134, 161, 237, 297, 410, 433
SfaNI GCATC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 73
SsiI CCGC 4 cut(s) 246, 249, 318, 402
SspMI CTAG 2 cut(s) 74, 195
StyI CCWWGG 4 cut(s) 108, 148, 187, 231
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 78
TauI GCSGC 2 cut(s) 248, 251
TscAI CASTG 1 cut(s) 404
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 5 cut(s) 113, 177, 273, 357, 408
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 17, 407
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 404
VneI GTGCAC 1 cut(s) 374
XagI CCTNNNNNAGG 1 cut(s) 163
XspI CTAG 2 cut(s) 74, 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.