FvH4_3g01660

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000301
Physical Location & Seq
Forward (+)
858564 .. 860252
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01660.t1

Sequence Viewer

Length: 798 bp
ATGGCCCTTGTTTCAGCTGCAGTCGATCTAGACAATTACAACGAAGAAGTGGAAGAACCGGAAGTAGTGCAGAGCAATGAAGCAACTGTGCCTGAATCTGAAATGCAAGAAGCGACTACTTCTCTGAGAGGAGTGAGTCGCTTTCTGGCGTCGCAGCAGAACCCGTTGGGAAACTACACCTGTGACAAGTTTCATAGGGTTTGTCGTCTGAAGAAGAGCTCGGGGGCCGGACTGCTGCAAGAAGAAGGGGAAGTGTGTGAATGCATCGTTCGACAAAAGGCATTGCGGTGGATGCAACCACAAGTGCAAGAGAGGGGAGTTCTGGTGAACAACACAAAGTCCTTACGTATAGTAATGGCAATGATAATATCTAAAAATGTGTTCACCCTAATCTTGGTGCTATTGTTGGGGATAGCCATTGCCCTTTCAGCCACTGTCGAAGAGCTCCCCCAAGTCGATCCTAACGAGCTTCGGTTCACAACGTCATCTCTCCGAGGGATAGGTGGCCGGTTCCTTGCGGAGCAAGTACGAGCAGCACCAACAACATGTGACAAGAATCCGACGGTCTGTAAGAGCAAGGGCAGTGCAGGACCTAACTGCTGCAGCAAAAAATGCGTCAATATAAGGACGGACACAAACAATTGTGGGCGGTGTGGAGCGAAGTGTAAGTATTCGGAACTCTGTTGCAACGGTGTTTGTGTGAACCCGTCTGTCAATGGCAAACATTGTGGCAAGTGCGGCAACAAGTGTGGAAATGGAAGCTCATGCGTGTTTGGCCTGTGCAGCTACGCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

266

Amino Acids

28.6

Weight (kDa)

8.34

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 181 - 264 1.1e-27 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 286, 518, 649, 738
AclWI GGATC 1 cut(s) 452
AcoI YGGCCR 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 545
AluBI AGCT 6 cut(s) 17, 219, 445, 469, 762, 786
AluI AGCT 6 cut(s) 17, 219, 445, 469, 762, 786
Alw21I GWGCWC 2 cut(s) 221, 447
AlwI GGATC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 220
AoxI GGCC 4 cut(s) 3, 225, 505, 775
ApeKI GCWGC 7 cut(s) 17, 154, 235, 533, 600, 603, 783
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
AspS9I GGNCC 3 cut(s) 4, 225, 590
AsuHPI GGTGA 2 cut(s) 337, 376
AvaI CYCGRG 1 cut(s) 220
AvaII GGWCC 1 cut(s) 590
BanII GRGCYC 2 cut(s) 221, 447
Bbv12I GWGCWC 2 cut(s) 221, 447
BbvI GCAGC 6 cut(s) 4, 166, 222, 545, 587, 615
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 18, 601
BisI GCNGC 8 cut(s) 18, 155, 236, 534, 601, 604, 739, 784
BlsI GCNGC 8 cut(s) 19, 156, 237, 535, 602, 605, 740, 785
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 220
BmgT120I GGNCC 3 cut(s) 4, 225, 590
BmiI GGNNCC 2 cut(s) 226, 512
BmsI GCATC 2 cut(s) 273, 282
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 507
Bse3DI GCAATG 4 cut(s) 82, 281, 366, 417
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 4 cut(s) 82, 281, 366, 417
BseMII CTCAG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 6 cut(s) 4, 166, 222, 545, 587, 615
BsgI GTGCAG 2 cut(s) 89, 606
BshFI GGCC 4 cut(s) 5, 227, 507, 777
BsiHKAI GWGCWC 2 cut(s) 221, 447
BsiHKCI CYCGRG 1 cut(s) 220
BsiSI CCGG 3 cut(s) 59, 228, 508
BslI CCNNNNNNNGG 1 cut(s) 394
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 4 cut(s) 5, 227, 507, 777
BsoBI CYCGRG 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 221, 447
Bsp143I GATC 2 cut(s) 25, 457
BspACI CCGC 4 cut(s) 286, 518, 649, 738
BspANI GGCC 4 cut(s) 5, 227, 507, 777
BspCNI CTCAG 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 226, 512
BspMAI CTGCAG 2 cut(s) 22, 605
BspPI GGATC 1 cut(s) 452
BspQI GCTCTTC 2 cut(s) 209, 435
BsrDI GCAATG 4 cut(s) 82, 281, 366, 417
BsrFI RCCGGY 1 cut(s) 507
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 2 cut(s) 25, 457
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 4 cut(s) 88, 436, 565, 692
Bst6I CTCTTC 2 cut(s) 209, 435
BstACI GRCGYC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 612
BstBAI YACGTR 1 cut(s) 347
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 297
BstKTI GATC 2 cut(s) 28, 460
BstMBI GATC 2 cut(s) 25, 457
BstMWI GCNNNNNNNGC 6 cut(s) 292, 428, 612, 738, 774, 783
BstNSI RCATGY 1 cut(s) 549
BstSFI CTRYAG 2 cut(s) 18, 601
BstSNI TACGTA 1 cut(s) 347
BstV1I GCAGC 6 cut(s) 4, 166, 222, 545, 587, 615
BsuRI GGCC 4 cut(s) 5, 227, 507, 777
BtsCI GGATG 1 cut(s) 297
BtsI GCAGTG 1 cut(s) 589
BtsIMutI CAGTG 2 cut(s) 432, 589
CaiI CAGNNNCTG 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 3 cut(s) 4, 225, 590
CseI GACGC 2 cut(s) 138, 604
Csp6I GTAC 1 cut(s) 527
CspCI CAANNNNNGTGG 4 cut(s) 709, 730, 744, 765
CviAII CATG 2 cut(s) 546, 765
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 1 cut(s) 125
DpnI GATC 2 cut(s) 27, 459
DpnII GATC 2 cut(s) 25, 457
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 2 cut(s) 209, 435
EarI CTCTTC 2 cut(s) 209, 435
Ecl136II GAGCTC 2 cut(s) 219, 445
Eco105I TACGTA 1 cut(s) 347
Eco24I GRGCYC 2 cut(s) 221, 447
Eco47I GGWCC 1 cut(s) 590
Eco53kI GAGCTC 2 cut(s) 219, 445
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 220
EcoICRI GAGCTC 2 cut(s) 219, 445
EcoO109I RGGNCCY 1 cut(s) 590
EcoT22I ATGCAT 1 cut(s) 266
EcoT38I GRGCYC 2 cut(s) 221, 447
FaeI CATG 2 cut(s) 549, 768
FaiI YATR 5 cut(s) 195, 350, 547, 623, 766
FatI CATG 2 cut(s) 545, 764
Fnu4HI GCNGC 8 cut(s) 18, 155, 236, 534, 601, 604, 739, 784
FokI GGATG 1 cut(s) 304
FriOI GRGCYC 2 cut(s) 221, 447
Fsp4HI GCNGC 8 cut(s) 18, 155, 236, 534, 601, 604, 739, 784
FspBI CTAG 1 cut(s) 29
GluI GCNGC 8 cut(s) 18, 155, 236, 534, 601, 604, 739, 784
HaeIII GGCC 4 cut(s) 5, 227, 507, 777
HapII CCGG 3 cut(s) 59, 228, 508
HgaI GACGC 2 cut(s) 138, 604
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 549, 768
HinfI GANTC 3 cut(s) 95, 136, 556
HpaII CCGG 3 cut(s) 59, 228, 508
HphI GGTGA 2 cut(s) 337, 376
Hpy166II GTNNAC 4 cut(s) 328, 384, 477, 703
Hpy188I TCNGA 6 cut(s) 100, 126, 210, 494, 561, 676
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 4 cut(s) 328, 384, 477, 703
Hpy99I CGWCG 2 cut(s) 154, 565
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 4 cut(s) 88, 436, 565, 692
HpyCH4IV ACGT 2 cut(s) 346, 482
HpyF10VI GCNNNNNNNGC 6 cut(s) 292, 428, 612, 738, 774, 783
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 2 cut(s) 346, 482
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 549, 768
Kzo9I GATC 2 cut(s) 25, 457
LguI GCTCTTC 2 cut(s) 209, 435
LmnI GCTCC 3 cut(s) 450, 520, 656
LpnPI CCDG 9 cut(s) 72, 105, 131, 193, 241, 308, 521, 573, 791
Lsp1109I GCAGC 6 cut(s) 4, 166, 222, 545, 587, 615
LweI GCATC 2 cut(s) 273, 282
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 346, 482
MaeIII GTNAC 2 cut(s) 182, 548
MalI GATC 2 cut(s) 27, 459
MboI GATC 2 cut(s) 25, 457
MboII GAAGA 6 cut(s) 56, 65, 223, 226, 254, 452
MfeI CAATTG 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 221, 447
MluCI AATT 3 cut(s) 34, 640, 793
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 584
MnlI CCTC 3 cut(s) 122, 306, 488
Mph1103I ATGCAT 1 cut(s) 266
MslI CAYNNNNRTG 1 cut(s) 286
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 59, 228, 508
MunI CAATTG 1 cut(s) 640
Mva1269I GAATGC 1 cut(s) 266
MwoI GCNNNNNNNGC 6 cut(s) 292, 428, 612, 738, 774, 783
NdeII GATC 2 cut(s) 25, 457
NlaIII CATG 2 cut(s) 549, 768
NlaIV GGNNCC 2 cut(s) 226, 512
NmuCI GTSAC 2 cut(s) 182, 548
NsiI ATGCAT 1 cut(s) 266
NspI RCATGY 1 cut(s) 549
PciI ACATGT 1 cut(s) 545
PciSI GCTCTTC 2 cut(s) 209, 435
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 95, 556
PkrI GCNGC 8 cut(s) 19, 156, 237, 535, 602, 605, 740, 785
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 590
PscI ACATGT 1 cut(s) 545
Psp124BI GAGCTC 2 cut(s) 221, 447
Psp5II RGGWCCY 1 cut(s) 590
PspN4I GGNNCC 2 cut(s) 226, 512
PspPI GGNCC 3 cut(s) 4, 225, 590
PspPPI RGGWCCY 1 cut(s) 590
PstI CTGCAG 2 cut(s) 22, 605
PstNI CAGNNNCTG 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
RseI CAYNNNNRTG 1 cut(s) 286
SacI GAGCTC 2 cut(s) 221, 447
SapI GCTCTTC 2 cut(s) 209, 435
SatI GCNGC 8 cut(s) 18, 155, 236, 534, 601, 604, 739, 784
Sau3AI GATC 2 cut(s) 25, 457
Sau96I GGNCC 3 cut(s) 4, 225, 590
SchI GAGTC 1 cut(s) 145
SduI GDGCHC 2 cut(s) 221, 447
SfaNI GCATC 2 cut(s) 273, 282
SfcI CTRYAG 2 cut(s) 18, 601
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 286
SnaBI TACGTA 1 cut(s) 347
Sse9I AATT 3 cut(s) 34, 640, 793
SsiI CCGC 4 cut(s) 286, 518, 649, 738
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 2 cut(s) 221, 447
TaaI ACNGT 4 cut(s) 88, 436, 565, 692
TaiI ACGT 2 cut(s) 349, 485
TaqI TCGA 4 cut(s) 24, 271, 438, 456
TasI AATT 3 cut(s) 34, 640, 793
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 2 cut(s) 95, 556
TscAI CASTG 2 cut(s) 439, 589
TseFI GTSAC 2 cut(s) 182, 548
TseI GCWGC 7 cut(s) 17, 154, 235, 533, 600, 603, 783
Tsp45I GTSAC 2 cut(s) 182, 548
TspDTI ATGAA 2 cut(s) 93, 182
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 2 cut(s) 439, 589
VpaK11BI GGWCC 1 cut(s) 590
XbaI TCTAGA 1 cut(s) 28
XceI RCATGY 1 cut(s) 549
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.