Rmu_sc0001717.1_g000006

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001717.1
OG0000301
Physical Location & Seq
Forward (+)
19709 .. 22260
2552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001717.1_g000006.1.cds

Sequence Viewer

Length: 753 bp
atgaagttgctccacatctttgcactcctagcaacaatggctgttgtttcagctgcgtttgatctggaagattacactgaagaaccggaagtgcagagtaatgaatcaacggtgcctgaatctgaaatgcaagaagcgactacttctttgagaggagtgagccgctttctgacttcccagcagaacctgttgggaaacttgacctgcaacaagtttcctagggtttgtcgtctgaagaagagcccagggcccgactgctgcaagaagaagtgtgtcaatgtgaagacggataaattgaactgcggggtctgcggttacaaatgcaagtaccctgagatttgttgcaagggaaagtgcgtcaatgtgaagaaggataaattgaactgcgggatctgcggttacaagtgcaagtaccctgagatttgctgcaaggggaagcgtgattcaaaccatggtgaaggtgaatgcagcctgtctccccttccgctagagcaacgggcgttttctgatcagacaccgatcaagacaccaaacggttctatatggaagtcgtcatcactggcgctatcaataagtgatgggttcgtcaacaccgtcttctggatccagaccattctcggccgaagcttaggtcgggtacagggcaggcacatcgcacatagatcgaactccgatatgaccagatcagacaccgatcaagacaccgaacggttctatatggaagtcgtcatcaccggcgctatcaacaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

250

Amino Acids

27.77

Weight (kDa)

8.7

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 112
AciI CCGC 6 cut(s) 163, 303, 312, 387, 396, 485
AclWI GGATC 3 cut(s) 398, 598, 611
AcoI YGGCCR 1 cut(s) 619
AcuI CTGAAG 2 cut(s) 99, 254
AfaI GTAC 3 cut(s) 329, 413, 639
AfiI CCNNNNNNNGG 1 cut(s) 600
AgsI TTSAA 3 cut(s) 298, 382, 447
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 53, 627
AluI AGCT 2 cut(s) 53, 627
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 3 cut(s) 398, 598, 611
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 2 cut(s) 248, 619
ApaI GGGCCC 1 cut(s) 252
ApeKI GCWGC 4 cut(s) 53, 258, 426, 468
ArsI GACNNNNNNTTYG 4 cut(s) 130, 162, 616, 648
AspA2I CCTAGG 1 cut(s) 218
AspLEI GCGC 2 cut(s) 565, 740
AspS9I GGNCC 2 cut(s) 248, 249
AsuHPI GGTGA 3 cut(s) 467, 473, 724
AvrII CCTAGG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 252
BamHI GGATCC 1 cut(s) 603
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 2 cut(s) 245, 252
BbsI GAAGAC 2 cut(s) 290, 589
BbvI GCAGC 4 cut(s) 40, 245, 413, 480
BccI CCATC 1 cut(s) 572
BcgI CGANNNNNNTGC 4 cut(s) 634, 645, 668, 679
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 3 cut(s) 29, 219, 488
BfoI RGCGCY 2 cut(s) 566, 741
BfuAI ACCTGC 1 cut(s) 212
BisI GCNGC 5 cut(s) 54, 163, 259, 427, 469
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 5 cut(s) 55, 164, 260, 428, 470
Bme1390I CCNGG 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 248, 249
BmiI GGNNCC 3 cut(s) 114, 250, 605
BmrFI CCNGG 1 cut(s) 246
BpiI GAAGAC 2 cut(s) 290, 589
Bpu10I CCTNAGC 1 cut(s) 628
BsaJI CCNNGG 4 cut(s) 218, 244, 245, 451
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 1 cut(s) 600
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 218, 244, 245, 451
BseLI CCNNNNNNNGG 1 cut(s) 600
BseMII CTCAG 2 cut(s) 324, 408
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 252
BseX3I CGGCCG 1 cut(s) 619
BseXI GCAGC 4 cut(s) 40, 245, 413, 480
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 622
BshFI GGCC 2 cut(s) 250, 621
BshNI GGYRCC 1 cut(s) 112
BsiEI CGRYCG 1 cut(s) 622
BsiSI CCGG 2 cut(s) 86, 735
BslI CCNNNNNNNGG 1 cut(s) 600
BsmAI GTCTC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 2 cut(s) 250, 621
Bsp120I GGGCCC 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 245, 252
Bsp143I GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 6 cut(s) 163, 303, 312, 387, 396, 485
BspANI GGCC 2 cut(s) 250, 621
BspCNI CTCAG 2 cut(s) 325, 409
BspLI GGNNCC 3 cut(s) 114, 250, 605
BspMI ACCTGC 1 cut(s) 212
BspPI GGATC 3 cut(s) 398, 598, 611
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 4 cut(s) 218, 244, 245, 451
BssMI GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
BssT1I CCWWGG 2 cut(s) 218, 451
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 4 cut(s) 112, 536, 595, 711
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 3 cut(s) 333, 417, 628
BstDSI CCRYGG 1 cut(s) 451
BstH2I RGCGCY 2 cut(s) 566, 741
BstHHI GCGC 2 cut(s) 565, 740
BstKTI GATC 8 cut(s) 64, 393, 511, 522, 606, 665, 686, 697
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 4 cut(s) 29, 38, 309, 393
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 4 cut(s) 40, 245, 413, 480
BstV2I GAAGAC 2 cut(s) 290, 589
BstX2I RGATCY 2 cut(s) 390, 603
BstYI RGATCY 2 cut(s) 390, 603
BstZI CGGCCG 1 cut(s) 619
BsuRI GGCC 2 cut(s) 250, 621
BtgI CCRYGG 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 637
BtsIMutI CAGTG 2 cut(s) 75, 557
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 187
CfoI GCGC 2 cut(s) 565, 740
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 2 cut(s) 248, 249
CseI GACGC 1 cut(s) 346
Csp6I GTAC 3 cut(s) 328, 412, 638
CviAII CATG 1 cut(s) 452
CviJI RGCY 8 cut(s) 41, 53, 162, 243, 250, 471, 621, 627
CviKI_1 RGCY 8 cut(s) 41, 53, 162, 243, 250, 471, 621, 627
CviQI GTAC 3 cut(s) 328, 412, 638
DdeI CTNAG 3 cut(s) 333, 417, 628
DpnI GATC 8 cut(s) 63, 392, 510, 521, 605, 664, 685, 696
DpnII GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
EaeI YGGCCR 1 cut(s) 619
EagI CGGCCG 1 cut(s) 619
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
EclXI CGGCCG 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 218, 451
Eco24I GRGCYC 2 cut(s) 245, 252
Eco52I CGGCCG 1 cut(s) 619
Eco57I CTGAAG 2 cut(s) 99, 254
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 218, 451
EcoT38I GRGCYC 2 cut(s) 245, 252
ErhI CCWWGG 2 cut(s) 218, 451
FaeI CATG 1 cut(s) 455
FaiI YATR 7 cut(s) 453, 542, 544, 660, 677, 717, 719
FatI CATG 1 cut(s) 451
FauI CCCGC 2 cut(s) 296, 380
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 5 cut(s) 54, 163, 259, 427, 469
FriOI GRGCYC 2 cut(s) 245, 252
Fsp4HI GCNGC 5 cut(s) 54, 163, 259, 427, 469
FspBI CTAG 3 cut(s) 29, 219, 488
GlaI GCGC 2 cut(s) 564, 739
GluI GCNGC 5 cut(s) 54, 163, 259, 427, 469
GsaI CCCAGC 1 cut(s) 181
HaeII RGCGCY 2 cut(s) 566, 741
HaeIII GGCC 2 cut(s) 250, 621
HapII CCGG 2 cut(s) 86, 735
HgaI GACGC 1 cut(s) 346
HhaI GCGC 2 cut(s) 565, 740
Hin1II CATG 1 cut(s) 455
Hin6I GCGC 2 cut(s) 563, 738
HinP1I GCGC 2 cut(s) 563, 738
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 625
HinfI GANTC 3 cut(s) 104, 119, 443
HpaII CCGG 2 cut(s) 86, 735
HphI GGTGA 3 cut(s) 467, 473, 724
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 7 cut(s) 124, 171, 234, 508, 513, 673, 688
Hpy188III TCNNGA 5 cut(s) 65, 523, 601, 607, 698
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 3 cut(s) 364, 452, 491
HpyCH4III ACNGT 4 cut(s) 112, 536, 595, 711
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 38, 309, 393
HpyF3I CTNAG 3 cut(s) 333, 417, 628
Hsp92II CATG 1 cut(s) 455
HspAI GCGC 2 cut(s) 563, 738
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 15
Lsp1109I GCAGC 4 cut(s) 40, 245, 413, 480
MaeI CTAG 3 cut(s) 29, 219, 488
MaeIII GTNAC 2 cut(s) 314, 398
MalI GATC 8 cut(s) 63, 392, 510, 521, 605, 664, 685, 696
MboI GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
MboII GAAGA 8 cut(s) 80, 92, 247, 250, 277, 295, 379, 589
MflI RGATCY 2 cut(s) 390, 603
MhlI GDGCHC 2 cut(s) 245, 252
MluCI AATT 2 cut(s) 293, 377
MnlI CCTC 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 86, 735
MspR9I CCNGG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 4 cut(s) 29, 38, 309, 393
NcoI CCATGG 1 cut(s) 451
NdeII GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 3 cut(s) 114, 250, 605
NmeAIII GCCGAG 1 cut(s) 597
PasI CCCWGGG 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 104, 119, 443
PkrI GCNGC 5 cut(s) 55, 164, 260, 428, 470
Psp6I CCWGG 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 114, 250, 605
PspOMI GGGCCC 1 cut(s) 248
PspPI GGNCC 2 cut(s) 248, 249
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 2 cut(s) 390, 603
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 3 cut(s) 329, 413, 639
RsaNI GTAC 3 cut(s) 328, 412, 638
SapI GCTCTTC 1 cut(s) 233
SatI GCNGC 5 cut(s) 54, 163, 259, 427, 469
Sau3AI GATC 8 cut(s) 61, 390, 508, 519, 603, 662, 683, 694
Sau96I GGNCC 2 cut(s) 248, 249
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 2 cut(s) 245, 252
SetI ASST 6 cut(s) 55, 189, 206, 463, 629, 634
SgrAI CRCCGGYG 1 cut(s) 734
Sse9I AATT 2 cut(s) 293, 377
SsiI CCGC 6 cut(s) 163, 303, 312, 387, 396, 485
SspMI CTAG 3 cut(s) 29, 219, 488
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 2 cut(s) 218, 451
TaaI ACNGT 4 cut(s) 112, 536, 595, 711
TaqI TCGA 1 cut(s) 665
TasI AATT 2 cut(s) 293, 377
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 3 cut(s) 104, 119, 443
TscAI CASTG 2 cut(s) 82, 564
TseI GCWGC 4 cut(s) 53, 258, 426, 468
TspDTI ATGAA 2 cut(s) 17, 117
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 2 cut(s) 82, 564
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 3 cut(s) 29, 219, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.