FvH4_3g01640

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000301
Physical Location & Seq
Forward (+)
856439 .. 857203
765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01640.t1

Sequence Viewer

Length: 453 bp
ATGAAGTTGCTCCACATCTTTACAGTCCTAGCAACAATGGCCCTTGTTTCAGCTGCATTCGATCTAGACGATTACAACGAAGAACCGGAAATGCAGAGTACTGAATCAACTGTGCCTGAATCTGAAATGCACGAGGCGACTACTTCTCTGAGAGGAGTGAGTCGCTTTCTGGCGTCGCAGCAGAACCTGTTGGGAAACTACACCTGTGACAAGTTTCCTAGGGTTTGTCGTCTGAAGAAGAGCTCGGGGCCGGACTGCTGCAAGAAGAAGTGTGTCAATGTCAAGACTGATAGATTGAACTGCGGGATGTGCGGTTACAAGTGCAAGTACACTGAGATTTGCTGCAGGGGGAAGTGCGTCAATGCATCTTTTGATAAAAGGCATTGCGGTGGATGCAACCAGAAGTGCAAGAGAGGGGAGTTCTGTGTTTATGGGATGTGCAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

151

Amino Acids

16.84

Weight (kDa)

8.57

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 67 - 150 6.7e-30 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 303, 312, 387
AcuI CTGAAG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 2 cut(s) 100, 329
AgsI TTSAA 1 cut(s) 298
AluBI AGCT 2 cut(s) 53, 243
AluI AGCT 2 cut(s) 53, 243
Alw21I GWGCWC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 2 cut(s) 39, 248
ApeKI GCWGC 4 cut(s) 53, 178, 258, 342
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 40, 248
AvaI CYCGRG 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 218
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 4 cut(s) 40, 190, 245, 329
BfaI CTAG 3 cut(s) 29, 65, 219
BfmI CTRYAG 1 cut(s) 343
BisI GCNGC 4 cut(s) 54, 179, 259, 343
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 4 cut(s) 55, 180, 260, 344
BmcAI AGTACT 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 40, 248
BmiI GGNNCC 1 cut(s) 249
BmsI GCATC 2 cut(s) 374, 383
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 218
BsaWI WCCGGW 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 3 cut(s) 312, 398, 441
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 2 cut(s) 140, 324
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 4 cut(s) 40, 190, 245, 329
BshFI GGCC 2 cut(s) 41, 250
BsiHKAI GWGCWC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 2 cut(s) 86, 251
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 2 cut(s) 41, 250
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 3 cut(s) 303, 312, 387
BspANI GGCC 2 cut(s) 41, 250
BspCNI CTCAG 2 cut(s) 141, 325
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 347
BspQI GCTCTTC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 173
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 25, 112
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 149, 333
BstF5I GGATG 3 cut(s) 312, 398, 441
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 38, 309, 393
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 4 cut(s) 40, 190, 245, 329
BsuRI GGCC 2 cut(s) 41, 250
BtsCI GGATG 3 cut(s) 312, 398, 441
BtsIMutI CAGTG 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 40, 248
CseI GACGC 2 cut(s) 162, 346
Csp6I GTAC 2 cut(s) 99, 328
CviAII CATG 1 cut(s) 450
CviJI RGCY 4 cut(s) 41, 53, 243, 250
CviKI_1 RGCY 4 cut(s) 41, 53, 243, 250
CviQI GTAC 2 cut(s) 99, 328
DdeI CTNAG 2 cut(s) 149, 333
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 218
EcoT22I ATGCAT 2 cut(s) 367, 451
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 1 cut(s) 453
FaiI YATR 3 cut(s) 432, 447, 451
FatI CATG 1 cut(s) 449
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 54, 179, 259, 343
FokI GGATG 3 cut(s) 319, 405, 448
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 4 cut(s) 54, 179, 259, 343
FspBI CTAG 3 cut(s) 29, 65, 219
GluI GCNGC 4 cut(s) 54, 179, 259, 343
HaeIII GGCC 2 cut(s) 41, 250
HapII CCGG 2 cut(s) 86, 251
HgaI GACGC 2 cut(s) 162, 346
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 1 cut(s) 453
HinfI GANTC 3 cut(s) 104, 119, 160
HpaII CCGG 2 cut(s) 86, 251
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 3 cut(s) 124, 150, 234
Hpy188III TCNNGA 2 cut(s) 65, 283
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 178
HpyCH4III ACNGT 2 cut(s) 25, 112
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 309, 393
HpyF3I CTNAG 2 cut(s) 149, 333
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 1 cut(s) 453
Kzo9I GATC 1 cut(s) 61
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 15
LpnPI CCDG 8 cut(s) 99, 129, 155, 200, 217, 264, 331, 413
Lsp1109I GCAGC 4 cut(s) 40, 190, 245, 329
LweI GCATC 2 cut(s) 374, 383
MaeI CTAG 3 cut(s) 29, 65, 219
MaeIII GTNAC 2 cut(s) 206, 314
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 4 cut(s) 92, 247, 250, 277
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 1 cut(s) 442
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 3 cut(s) 127, 146, 407
Mph1103I ATGCAT 2 cut(s) 367, 451
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 86, 251
Mva1269I GAATGC 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 38, 309, 393
NdeII GATC 1 cut(s) 61
NlaIII CATG 1 cut(s) 453
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 206
NsiI ATGCAT 2 cut(s) 367, 451
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 75
PctI GAATGC 1 cut(s) 56
PfeI GAWTC 2 cut(s) 104, 119
PkrI GCNGC 4 cut(s) 55, 180, 260, 344
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 40, 248
PstI CTGCAG 1 cut(s) 347
PstNI CAGNNNCTG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 2 cut(s) 100, 329
RsaNI GTAC 2 cut(s) 99, 328
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 233
SatI GCNGC 4 cut(s) 54, 179, 259, 343
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 2 cut(s) 40, 248
ScaI AGTACT 1 cut(s) 100
SchI GAGTC 1 cut(s) 169
SduI GDGCHC 1 cut(s) 245
SetI ASST 4 cut(s) 55, 189, 206, 245
SfaNI GCATC 2 cut(s) 374, 383
SfcI CTRYAG 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 1 cut(s) 442
SsiI CCGC 3 cut(s) 303, 312, 387
SspMI CTAG 3 cut(s) 29, 65, 219
SstI GAGCTC 1 cut(s) 245
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 2 cut(s) 25, 112
TaqI TCGA 1 cut(s) 60
TasI AATT 1 cut(s) 442
TatI WGTACW 2 cut(s) 98, 327
TfiI GAWTC 2 cut(s) 104, 119
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 4 cut(s) 53, 178, 258, 342
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 337
XbaI TCTAGA 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 3 cut(s) 29, 65, 219
ZrmI AGTACT 1 cut(s) 100
Zsp2I ATGCAT 2 cut(s) 367, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.