Rroxscaffold_1G00049920

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000301
Physical Location & Seq
Forward (+)
70219955 .. 70224150
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049920.1

Sequence Viewer

Length: 747 bp
ATGGCCATTGTTTCAGCTGAATATCTAGACGATTACAACAATGAAGAAATGCAAATTCAAAATACCGAATCAACAGTGTCTGAAATGCAAGAAGCAACAACTTCTTTGAGAGGGGTAAGCCGCTTCCTGACCCAGCAGAACCTGTTGGCGAGCGTGACTTGCGACAAGTTACCTAGGGTTTGTCGTCTGAAGAAGAGCCCGGGGCCTGACTGCTGCAAGAAGAAGTGTGTGAATTTGAAGAGGGATAGATTGAATTGCGGGATGTGCGGGTACAAGTGCAAGTACACTGAGATTTGCTGCAGGGGAAAATGCCTGAATGCATCGTTTGACGAAAAGCATTGCGGTGGATGCAACCAGAAGTGCAAGAAAGGGGAGTTTTGTGTTTTGGGGATAACTCGCCGGAGAGACTCAGACCTTCTCACTGACGTTTCTCCCTCCCCGTCCGATGCATGGGCAATCGAAGACCACAGGCGTGACGGCGACGACAAGAGGAAGAGGTCGGTGGTCTTGAGTCGTCGATCGGTGGCCGGACGGCGTCACTCCGGTCGGTCGCTGGCTGGTTCGCGCTGTGTCCGGTTGTTATCCACCAAAGTGGTATTAGAGCCGAAGGCTCGATCTTATGGTGTGTTAGCATTGGTTGTTGCGGTTATTGTTGCAAGGTGGTGGAAGAAATCTATGGTGTATGAAGTATTGCTGATGGGCAACGAAATGAGAGTGCAGATGAAAAAAAGAGAGGAGAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

248

Amino Acids

28.08

Weight (kDa)

9.44

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 52 - 131 1.1e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 565
AciI CCGC 5 cut(s) 121, 258, 267, 342, 644
AcoI YGGCCR 2 cut(s) 3, 525
AcsI RAATTY 2 cut(s) 54, 232
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 535
AfaI GTAC 2 cut(s) 272, 284
AfiI CCNNNNNNNGG 1 cut(s) 450
AgsI TTSAA 3 cut(s) 59, 238, 253
AleI CACNNNNGTG 2 cut(s) 471, 590
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 399
AlwNI CAGNNNCTG 2 cut(s) 80, 142
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 3 cut(s) 3, 203, 525
ApeKI GCWGC 2 cut(s) 213, 297
ApoI RAATTY 2 cut(s) 54, 232
AspA2I CCTAGG 1 cut(s) 173
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 2 cut(s) 200, 201
AvaI CYCGRG 1 cut(s) 199
AvrII CCTAGG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 200, 284
BccI CCATC 1 cut(s) 691
BceAI ACGGC 2 cut(s) 493, 548
BcnI CCSGG 2 cut(s) 200, 201
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 2 cut(s) 26, 174
BfmI CTRYAG 1 cut(s) 298
BisI GCNGC 3 cut(s) 121, 214, 298
BlnI CCTAGG 1 cut(s) 173
BlsI GCNGC 3 cut(s) 122, 215, 299
Bme1390I CCNGG 2 cut(s) 200, 201
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 200, 201
BmsI GCATC 3 cut(s) 329, 338, 436
BpiI GAAGAC 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 2 cut(s) 200, 201
BsaHI GRCGYC 1 cut(s) 535
BsaJI CCNNGG 3 cut(s) 173, 199, 200
BsaWI WCCGGW 2 cut(s) 542, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 173, 199, 200
BseGI GGATG 2 cut(s) 267, 353
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 279, 423
BseXI GCAGC 2 cut(s) 200, 284
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 1 cut(s) 565
Bsh1285I CGRYCG 3 cut(s) 521, 547, 551
BshFI GGCC 3 cut(s) 5, 205, 527
BsiEI CGRYCG 3 cut(s) 521, 547, 551
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 5 cut(s) 200, 400, 528, 543, 574
BslI CCNNNNNNNGG 1 cut(s) 450
BsmAI GTCTC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 3 cut(s) 5, 205, 527
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 2 cut(s) 518, 614
BspACI CCGC 5 cut(s) 121, 258, 267, 342, 644
BspANI GGCC 3 cut(s) 5, 205, 527
BspCNI CTCAG 2 cut(s) 280, 422
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 302
BspQI GCTCTTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 173, 199, 200
BssMI GATC 2 cut(s) 518, 614
BssNI GRCGYC 1 cut(s) 535
BssT1I CCWWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 3 cut(s) 188, 233, 488
BstACI GRCGYC 1 cut(s) 535
BstC8I GCNNGC 2 cut(s) 151, 555
BstDEI CTNAG 2 cut(s) 288, 409
BstF5I GGATG 2 cut(s) 267, 353
BstFNI CGCG 1 cut(s) 565
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 2 cut(s) 521, 617
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 2 cut(s) 518, 614
BstMCI CGRYCG 3 cut(s) 521, 547, 551
BstMWI GCNNNNNNNGC 3 cut(s) 159, 264, 348
BstSCI CCNGG 2 cut(s) 198, 199
BstSFI CTRYAG 1 cut(s) 298
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 2 cut(s) 200, 284
BstV2I GAAGAC 1 cut(s) 468
BstXI CCANNNNNNTGG 1 cut(s) 592
BsuRI GGCC 3 cut(s) 5, 205, 527
BtsCI GGATG 2 cut(s) 267, 353
BtsIMutI CAGTG 3 cut(s) 81, 285, 420
Cac8I GCNNGC 2 cut(s) 151, 555
CaiI CAGNNNCTG 2 cut(s) 80, 142
CfoI GCGC 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 199
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 271, 283
CviAII CATG 1 cut(s) 450
CviJI RGCY 9 cut(s) 5, 17, 120, 198, 205, 527, 557, 604, 611
CviKI_1 RGCY 9 cut(s) 5, 17, 120, 198, 205, 527, 557, 604, 611
CviQI GTAC 2 cut(s) 271, 283
DdeI CTNAG 2 cut(s) 288, 409
DpnI GATC 2 cut(s) 520, 616
DpnII GATC 2 cut(s) 518, 614
EaeI YGGCCR 2 cut(s) 3, 525
Eam1104I CTCTTC 3 cut(s) 188, 233, 488
EarI CTCTTC 3 cut(s) 188, 233, 488
Eco130I CCWWGG 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 173
EcoT22I ATGCAT 2 cut(s) 322, 451
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 1 cut(s) 453
FaiI YATR 5 cut(s) 451, 621, 677, 684, 745
FatI CATG 1 cut(s) 449
FauI CCCGC 2 cut(s) 251, 260
Fnu4HI GCNGC 3 cut(s) 121, 214, 298
FokI GGATG 2 cut(s) 274, 360
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 3 cut(s) 121, 214, 298
FspBI CTAG 2 cut(s) 26, 174
GlaI GCGC 1 cut(s) 566
GluI GCNGC 3 cut(s) 121, 214, 298
GsaI CCCAGC 1 cut(s) 136
HaeIII GGCC 3 cut(s) 5, 205, 527
HapII CCGG 5 cut(s) 200, 400, 528, 543, 574
HgaI GACGC 1 cut(s) 524
HhaI GCGC 1 cut(s) 567
Hin1I GRCGYC 1 cut(s) 535
Hin1II CATG 1 cut(s) 453
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HinfI GANTC 3 cut(s) 68, 407, 511
HpaII CCGG 5 cut(s) 200, 400, 528, 543, 574
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 4 cut(s) 82, 189, 412, 445
Hpy188III TCNNGA 3 cut(s) 26, 127, 508
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 2 cut(s) 485, 519
HpyAV CCTTC 2 cut(s) 425, 601
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 264, 348
HpyF3I CTNAG 2 cut(s) 288, 409
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 535
Hsp92II CATG 1 cut(s) 453
HspAI GCGC 1 cut(s) 565
Kzo9I GATC 2 cut(s) 518, 614
LguI GCTCTTC 1 cut(s) 188
Lsp1109I GCAGC 2 cut(s) 200, 284
LweI GCATC 3 cut(s) 329, 338, 436
MaeI CTAG 2 cut(s) 26, 174
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 4 cut(s) 154, 168, 473, 536
MalI GATC 2 cut(s) 520, 616
MboI GATC 2 cut(s) 518, 614
MboII GAAGA 8 cut(s) 56, 202, 205, 232, 250, 473, 505, 679
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 54, 232, 253
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 401, 520
MnlI CCTC 6 cut(s) 104, 234, 445, 483, 489, 727
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 322, 451
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 342, 471, 590
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 5 cut(s) 200, 400, 528, 543, 574
MspR9I CCNGG 2 cut(s) 200, 201
Mva1269I GAATGC 1 cut(s) 322
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 3 cut(s) 159, 264, 348
NciI CCSGG 2 cut(s) 200, 201
NdeII GATC 2 cut(s) 518, 614
NlaIII CATG 1 cut(s) 453
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 3 cut(s) 154, 473, 536
NsiI ATGCAT 2 cut(s) 322, 451
OliI CACNNNNGTG 2 cut(s) 471, 590
PciSI GCTCTTC 1 cut(s) 188
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 534
PkrI GCNGC 3 cut(s) 122, 215, 299
Ple19I CGATCG 1 cut(s) 521
PleI GAGTC 2 cut(s) 401, 519
PpsI GAGTC 2 cut(s) 401, 519
PspFI CCCAGC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 203
PstI CTGCAG 1 cut(s) 302
PstNI CAGNNNCTG 2 cut(s) 80, 142
PsyI GACNNNGTC 1 cut(s) 534
PvuI CGATCG 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 272, 284
RsaNI GTAC 2 cut(s) 271, 283
RseI CAYNNNNRTG 3 cut(s) 342, 471, 590
SapI GCTCTTC 1 cut(s) 188
SatI GCNGC 3 cut(s) 121, 214, 298
Sau3AI GATC 2 cut(s) 518, 614
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 401, 520
ScrFI CCNGG 2 cut(s) 200, 201
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 19, 144, 175, 417, 429, 500, 662
SfaNI GCATC 3 cut(s) 329, 338, 436
SfcI CTRYAG 1 cut(s) 298
SmaI CCCGGG 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 342, 471, 590
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 3 cut(s) 54, 232, 253
SsiI CCGC 5 cut(s) 121, 258, 267, 342, 644
SspMI CTAG 2 cut(s) 26, 174
StyD4I CCNGG 2 cut(s) 198, 199
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 429
TaqI TCGA 3 cut(s) 459, 517, 613
TaqII GACCGA 1 cut(s) 537
TasI AATT 3 cut(s) 54, 232, 253
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 68
TscAI CASTG 3 cut(s) 81, 292, 427
TseFI GTSAC 3 cut(s) 154, 473, 536
TseI GCWGC 2 cut(s) 213, 297
Tsp45I GTSAC 3 cut(s) 154, 473, 536
TspDTI ATGAA 3 cut(s) 57, 699, 737
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 3 cut(s) 81, 292, 427
Tth111I GACNNNGTC 1 cut(s) 534
XapI RAATTY 2 cut(s) 54, 232
XbaI TCTAGA 1 cut(s) 25
XmaI CCCGGG 1 cut(s) 199
XmaJI CCTAGG 1 cut(s) 173
XspI CTAG 2 cut(s) 26, 174
Zsp2I ATGCAT 2 cut(s) 322, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.