Rh5CG310700

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000301
Physical Location & Seq
Reverse (-)
34294345 .. 34294767
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG310700.1

Sequence Viewer

Length: 423 bp
ATGAAGCTCAGCATCACTAATTGTTTCCTCATTTCTATTCTCCTCTTTAGTATTTTACTAATGAATATCGAAGCAAAGCCCACTGTACAGGAAGAGAAAGATGATCATCAAGAAGTCGCCATTGATGATTCTCTGAACTCTCCTGACAATGAAACTGAATCAAACTCCTTCTTCTTACACAGAAATGACCGCTCTCTGAGGCAAAAGATGAGAACACATACGGTGCGGTTGACCTGTAACAAGTTCCCCAGGATATGTTATACTAAGGGAAGGCCAGGACCTCATTGCTGCAAGAAGAAGTGTGTTAATGTTTTAACAGACAAGCTTAATTGTGGGAAATGTGGAAAGAAATGCAAGTACAGTGAGGTTTGCTGCAAAGAGAAATGTGTGAATCCATCACCGGAGTCATTGTGGTGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

140

Amino Acids

16.17

Weight (kDa)

8.83

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 78 - 133 4.3e-17 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 192
AciI CCGC 2 cut(s) 190, 226
AfaI GTAC 2 cut(s) 87, 359
AjnI CCWGG 2 cut(s) 248, 274
AluBI AGCT 2 cut(s) 7, 325
AluI AGCT 2 cut(s) 7, 325
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 288, 372
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 390
AvaII GGWCC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 275, 359
BccI CCATC 1 cut(s) 403
BciT130I CCWGG 2 cut(s) 250, 276
BclI TGATCA 1 cut(s) 103
BisI GCNGC 2 cut(s) 289, 373
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 2 cut(s) 290, 374
Bme1390I CCNGG 2 cut(s) 250, 276
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 278
BmrFI CCNGG 2 cut(s) 250, 276
BmsI GCATC 1 cut(s) 21
Bpu1102I GCTNAGC 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 250, 276
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 1 cut(s) 423
BseJI GATNNNNATC 1 cut(s) 105
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 22, 188
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 275, 359
BshFI GGCC 1 cut(s) 274
BsiSI CCGG 1 cut(s) 401
BsnI GGCC 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 1 cut(s) 103
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 2 cut(s) 190, 226
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 2 cut(s) 21, 189
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 283
BsrGI TGTACA 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 1 cut(s) 103
Bst2UI CCWGG 2 cut(s) 250, 276
Bst4CI ACNGT 3 cut(s) 85, 223, 362
Bst6I CTCTTC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 8, 197, 264
BstF5I GGATG 1 cut(s) 423
BstKTI GATC 1 cut(s) 106
BstMBI GATC 1 cut(s) 103
BstNI CCWGG 2 cut(s) 250, 276
BstSCI CCNGG 2 cut(s) 248, 274
BstV1I GCAGC 2 cut(s) 275, 359
BsuRI GGCC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 81, 367
Cfr13I GGNCC 1 cut(s) 278
Csp6I GTAC 2 cut(s) 86, 358
CviJI RGCY 4 cut(s) 7, 79, 274, 325
CviKI_1 RGCY 4 cut(s) 7, 79, 274, 325
CviQI GTAC 2 cut(s) 86, 358
DdeI CTNAG 3 cut(s) 8, 197, 264
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 248, 274
FaiI YATR 3 cut(s) 219, 256, 261
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 289, 373
Fsp4HI GCNGC 2 cut(s) 289, 373
GluI GCNGC 2 cut(s) 289, 373
HaeIII GGCC 1 cut(s) 274
HapII CCGG 1 cut(s) 401
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 4 cut(s) 128, 158, 391, 404
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 2 cut(s) 135, 198
Hpy188III TCNNGA 2 cut(s) 110, 143
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 178, 264
HpyCH4III ACNGT 3 cut(s) 85, 223, 362
HpyCH4V TGCA 3 cut(s) 291, 354, 375
HpyF3I CTNAG 3 cut(s) 8, 197, 264
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LpnPI CCDG 8 cut(s) 74, 156, 235, 247, 261, 262, 288, 414
Lsp1109I GCAGC 2 cut(s) 275, 359
LweI GCATC 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 236
MalI GATC 1 cut(s) 105
MbiI CCGCTC 1 cut(s) 192
MboI GATC 1 cut(s) 103
MboII GAAGA 3 cut(s) 104, 163, 307
MluCI AATT 2 cut(s) 19, 328
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 5 cut(s) 38, 53, 192, 291, 358
MseI TTAA 3 cut(s) 306, 314, 327
MslI CAYNNNNRTG 2 cut(s) 183, 412
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 2 cut(s) 250, 276
MvaI CCWGG 2 cut(s) 250, 276
NdeII GATC 1 cut(s) 103
PfeI GAWTC 3 cut(s) 128, 158, 391
PkrI GCNGC 2 cut(s) 290, 374
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PpuMI RGGWCCY 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 248, 274
PspGI CCWGG 2 cut(s) 248, 274
PspPI GGNCC 1 cut(s) 278
PspPPI RGGWCCY 1 cut(s) 278
RsaI GTAC 2 cut(s) 87, 359
RsaNI GTAC 2 cut(s) 86, 358
RseI CAYNNNNRTG 2 cut(s) 183, 412
SaqAI TTAA 3 cut(s) 306, 314, 327
SatI GCNGC 2 cut(s) 289, 373
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 250, 276
SetI ASST 5 cut(s) 9, 236, 283, 327, 369
SfaNI GCATC 1 cut(s) 21
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 2 cut(s) 183, 412
Sse9I AATT 2 cut(s) 19, 328
SsiI CCGC 2 cut(s) 190, 226
StyD4I CCNGG 2 cut(s) 248, 274
TaaI ACNGT 3 cut(s) 85, 223, 362
TaqI TCGA 1 cut(s) 69
TasI AATT 2 cut(s) 19, 328
TatI WGTACW 2 cut(s) 85, 357
TfiI GAWTC 3 cut(s) 128, 158, 391
Tru1I TTAA 3 cut(s) 306, 314, 327
Tru9I TTAA 3 cut(s) 306, 314, 327
TscAI CASTG 2 cut(s) 88, 367
TseI GCWGC 2 cut(s) 288, 372
TspDTI ATGAA 3 cut(s) 17, 77, 165
TspRI CASTG 2 cut(s) 88, 367
VpaK11BI GGWCC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.