Rh5CG232500

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000301
Physical Location & Seq
Reverse (-)
24136031 .. 24136438
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG232500.1

Sequence Viewer

Length: 408 bp
ATGGCCATTGTTTCAGCTGAATATCTAGACGATTACAACAATGAAGAAATGCAAATTCAAAATACCGAATCAACAGTGTCTGAAATGCAAGAAGCAACAACTTCTTTGAGAGGGGTAAGCCGCTTCCTGACCCAGCAGAACCCGTTGGCGAGCGTGACTTGCGACAAGTTACCTAGGGTTTGTCGTCTGAAGAAGAGCCCGGGGTCTGACTGCTGCAAGAAGAAGTGTGTGAATTTGAAGACGGATAGATTGAATTGCGGGATGTGCGGGTACAAGTGCAAGTACACTGAGATTTGCTGCAGGGGAAAATGCGTGAATGCATCGTTTGACGAAAAGCATTGCGGTGGATGCAACCAGAAGTGCAAGAAAGGGGAGTTTTGTGTTTTGGGGATGTGCAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

135

Amino Acids

15.07

Weight (kDa)

8.45

Isoelectric Point (pI)

21.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 52 - 135 4.2e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 121, 258, 267, 342
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 54, 232
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 2 cut(s) 272, 284
AgsI TTSAA 3 cut(s) 59, 238, 253
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 213, 297
ApoI RAATTY 2 cut(s) 54, 232
AspA2I CCTAGG 1 cut(s) 173
AsuC2I CCSGG 2 cut(s) 200, 201
AvaI CYCGRG 1 cut(s) 199
AvrII CCTAGG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 200, 284
BcnI CCSGG 2 cut(s) 200, 201
BfaI CTAG 2 cut(s) 26, 174
BfmI CTRYAG 1 cut(s) 298
BisI GCNGC 3 cut(s) 121, 214, 298
BlnI CCTAGG 1 cut(s) 173
BlsI GCNGC 3 cut(s) 122, 215, 299
Bme1390I CCNGG 2 cut(s) 200, 201
BmeT110I CYCGRG 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 200, 201
BmsI GCATC 2 cut(s) 329, 338
BpiI GAAGAC 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 200, 201
BsaJI CCNNGG 3 cut(s) 173, 199, 200
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 173, 199, 200
BseGI GGATG 3 cut(s) 267, 353, 396
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 200, 284
BseYI CCCAGC 1 cut(s) 132
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 1 cut(s) 200
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 200
BspACI CCGC 4 cut(s) 121, 258, 267, 342
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 302
BspQI GCTCTTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 173, 199, 200
BssT1I CCWWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 267, 353, 396
BstMWI GCNNNNNNNGC 3 cut(s) 159, 264, 348
BstSCI CCNGG 2 cut(s) 198, 199
BstSFI CTRYAG 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 200, 284
BstV2I GAAGAC 1 cut(s) 245
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 267, 353, 396
BtsIMutI CAGTG 2 cut(s) 81, 285
Cac8I GCNNGC 1 cut(s) 151
CaiI CAGNNNCTG 1 cut(s) 80
Cfr9I CCCGGG 1 cut(s) 199
Csp6I GTAC 2 cut(s) 271, 283
CviAII CATG 1 cut(s) 405
CviJI RGCY 4 cut(s) 5, 17, 120, 198
CviKI_1 RGCY 4 cut(s) 5, 17, 120, 198
CviQI GTAC 2 cut(s) 271, 283
DdeI CTNAG 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 173
EcoT22I ATGCAT 2 cut(s) 322, 406
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 1 cut(s) 408
FaiI YATR 2 cut(s) 402, 406
FatI CATG 1 cut(s) 404
FauI CCCGC 2 cut(s) 251, 260
Fnu4HI GCNGC 3 cut(s) 121, 214, 298
FokI GGATG 3 cut(s) 274, 360, 403
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 3 cut(s) 121, 214, 298
FspBI CTAG 2 cut(s) 26, 174
GluI GCNGC 3 cut(s) 121, 214, 298
GsaI CCCAGC 1 cut(s) 136
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 200
Hin1II CATG 1 cut(s) 408
HinfI GANTC 1 cut(s) 68
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 3 cut(s) 82, 189, 208
Hpy188III TCNNGA 2 cut(s) 26, 127
Hpy8I GTNNAC 1 cut(s) 285
HpyCH4III ACNGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 264, 348
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 1 cut(s) 408
LguI GCTCTTC 1 cut(s) 188
LpnPI CCDG 5 cut(s) 140, 146, 213, 286, 368
Lsp1109I GCAGC 2 cut(s) 200, 284
LweI GCATC 2 cut(s) 329, 338
MaeI CTAG 2 cut(s) 26, 174
MaeIII GTNAC 2 cut(s) 154, 168
MboII GAAGA 5 cut(s) 56, 202, 205, 232, 250
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 54, 232, 253, 397
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 104
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 322, 406
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 342
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 200, 201
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 159, 264, 348
NciI CCSGG 2 cut(s) 200, 201
NlaIII CATG 1 cut(s) 408
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 2 cut(s) 322, 406
PciSI GCTCTTC 1 cut(s) 188
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 3 cut(s) 122, 215, 299
PspFI CCCAGC 1 cut(s) 132
PstI CTGCAG 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 272, 284
RsaNI GTAC 2 cut(s) 271, 283
RseI CAYNNNNRTG 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 188
SatI GCNGC 3 cut(s) 121, 214, 298
ScrFI CCNGG 2 cut(s) 200, 201
SduI GDGCHC 1 cut(s) 200
SetI ASST 2 cut(s) 19, 175
SfaNI GCATC 2 cut(s) 329, 338
SfcI CTRYAG 1 cut(s) 298
SmaI CCCGGG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 4 cut(s) 54, 232, 253, 397
SsiI CCGC 4 cut(s) 121, 258, 267, 342
SspMI CTAG 2 cut(s) 26, 174
StyD4I CCNGG 2 cut(s) 198, 199
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 1 cut(s) 76
TasI AATT 4 cut(s) 54, 232, 253, 397
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 68
TscAI CASTG 2 cut(s) 81, 292
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 213, 297
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 1 cut(s) 57
TspGWI ACGGA 1 cut(s) 257
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 2 cut(s) 81, 292
XapI RAATTY 2 cut(s) 54, 232
XbaI TCTAGA 1 cut(s) 25
XmaI CCCGGG 1 cut(s) 199
XmaJI CCTAGG 1 cut(s) 173
XspI CTAG 2 cut(s) 26, 174
Zsp2I ATGCAT 2 cut(s) 322, 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.