Rh5DG021100

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000301
Physical Location & Seq
Forward (+)
1530182 .. 1530622
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG021100.1

Sequence Viewer

Length: 441 bp
ATGAACTCTCATAAGGTTTTCCTTCTGCTAGCCATGCTGATGATGGCTTCAGCCGGTACGCTTTCTGCGACACTAGACGAAGAAGAATCATCATTCTTCAACGAGGATAACAACGTCCAAAACCAAGAGAAAACTTCTCTCAGGGGAACAAGCAGCGTCTTCTTTGCTTCTCGGTCAGTGACAGCGTCAACATGTGACAAAAACCCTAAGGTTTGTGGCGCGGCTGCCGCGGACGGCTCAGAGTGCTGCAGTAAGAAGTGCGTGGATTTGAAGACGGACAGACTCAATTGCGGCAAATGTGGGGTGAAATGCAAGCAGACAGAGATATGCTGCAATGGTCATATTGTGACTCCAATGTCTGACAAGAAAAACTGTGGGAGATGCAACAATGCTTGCAAGAGACGCAACTCATGCGCGTTTGGGATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

146

Amino Acids

15.71

Weight (kDa)

8.5

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 63 - 146 1.3e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 355
AccII CGCG 3 cut(s) 221, 230, 416
AciI CCGC 4 cut(s) 221, 228, 230, 291
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 100, 271
AluBI AGCT 1 cut(s) 432
AluI AGCT 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 394
ApeKI GCWGC 5 cut(s) 153, 224, 246, 330, 429
AspLEI GCGC 2 cut(s) 221, 416
AsuHPI GGTGA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 28
AxyI CCTNAGG 1 cut(s) 207
BbsI GAAGAC 2 cut(s) 151, 278
BbvI GCAGC 4 cut(s) 165, 211, 233, 317
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 2 cut(s) 29, 74
BfmI CTRYAG 1 cut(s) 247
BisI GCNGC 8 cut(s) 154, 222, 225, 228, 247, 292, 331, 430
BlsI GCNGC 8 cut(s) 155, 223, 226, 229, 248, 293, 332, 431
BmsI GCATC 1 cut(s) 371
BmtI GCTAGC 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 151, 278
BsaJI CCNNGG 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 53
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 429
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 2 cut(s) 154, 252
BseXI GCAGC 4 cut(s) 165, 211, 233, 317
Bsh1236I CGCG 3 cut(s) 221, 230, 416
BsiSI CCGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 394
BsmBI CGTCTC 1 cut(s) 394
BspACI CCGC 4 cut(s) 221, 228, 230, 291
BspCNI CTCAG 2 cut(s) 153, 251
BspFNI CGCG 3 cut(s) 221, 230, 416
BspMAI CTGCAG 1 cut(s) 251
BspOI GCTAGC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 411
BstC8I GCNNGC 3 cut(s) 30, 314, 394
BstDEI CTNAG 3 cut(s) 140, 207, 238
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 3 cut(s) 221, 230, 416
BstHHI GCGC 2 cut(s) 221, 416
BstMAI GTCTC 1 cut(s) 394
BstMWI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 402, 411
BstNSI RCATGY 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 3 cut(s) 221, 230, 416
BstV1I GCAGC 4 cut(s) 165, 211, 233, 317
BstV2I GAAGAC 2 cut(s) 151, 278
Bsu36I CCTNAGG 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 30, 314, 394
CfoI GCGC 2 cut(s) 221, 416
Cfr10I RCCGGY 1 cut(s) 53
Cfr42I CCGCGG 1 cut(s) 231
CseI GACGC 3 cut(s) 145, 174, 411
Csp6I GTAC 1 cut(s) 57
CviAII CATG 3 cut(s) 34, 192, 411
CviJI RGCY 6 cut(s) 32, 47, 53, 224, 237, 432
CviKI_1 RGCY 6 cut(s) 32, 47, 53, 224, 237, 432
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 140, 207, 238
DrdI GACNNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 439
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 3 cut(s) 37, 195, 414
FaiI YATR 8 cut(s) 12, 35, 193, 328, 342, 412, 435, 439
FatI CATG 3 cut(s) 33, 191, 410
Fnu4HI GCNGC 8 cut(s) 154, 222, 225, 228, 247, 292, 331, 430
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 8 cut(s) 154, 222, 225, 228, 247, 292, 331, 430
FspBI CTAG 2 cut(s) 29, 74
GlaI GCGC 2 cut(s) 220, 415
GluI GCNGC 8 cut(s) 154, 222, 225, 228, 247, 292, 331, 430
HapII CCGG 1 cut(s) 54
HgaI GACGC 3 cut(s) 145, 174, 411
HhaI GCGC 2 cut(s) 221, 416
Hin1II CATG 3 cut(s) 37, 195, 414
Hin6I GCGC 2 cut(s) 219, 414
HinP1I GCGC 2 cut(s) 219, 414
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 3 cut(s) 86, 282, 349
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 2 cut(s) 241, 361
Hpy8I GTNNAC 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 7 cut(s) 249, 312, 333, 384, 396, 429, 437
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 402, 411
HpyF3I CTNAG 3 cut(s) 140, 207, 238
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 3 cut(s) 37, 195, 414
HspAI GCGC 2 cut(s) 219, 414
KspI CCGCGG 1 cut(s) 231
LpnPI CCDG 2 cut(s) 67, 127
Lsp1109I GCAGC 4 cut(s) 165, 211, 233, 317
LweI GCATC 1 cut(s) 371
MaeI CTAG 2 cut(s) 29, 74
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 178, 194, 346
MboII GAAGA 5 cut(s) 88, 92, 95, 151, 283
MfeI CAATTG 1 cut(s) 286
MluCI AATT 1 cut(s) 286
MlyI GAGTC 2 cut(s) 276, 343
MnlI CCTC 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 439
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 54
MunI CAATTG 1 cut(s) 286
MvnI CGCG 3 cut(s) 221, 230, 416
MwoI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 402, 411
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 3 cut(s) 37, 195, 414
NmuCI GTSAC 3 cut(s) 178, 194, 346
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 195
PciI ACATGT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 1 cut(s) 86
PflFI GACNNNGTC 1 cut(s) 184
PkrI GCNGC 8 cut(s) 155, 223, 226, 229, 248, 293, 332, 431
PleI GAGTC 2 cut(s) 276, 343
PpsI GAGTC 2 cut(s) 276, 343
PscI ACATGT 1 cut(s) 191
PstI CTGCAG 1 cut(s) 251
PsyI GACNNNGTC 1 cut(s) 184
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 38
SacII CCGCGG 1 cut(s) 231
SatI GCNGC 8 cut(s) 154, 222, 225, 228, 247, 292, 331, 430
SchI GAGTC 2 cut(s) 276, 343
SetI ASST 4 cut(s) 18, 117, 213, 434
SfaNI GCATC 1 cut(s) 371
SfcI CTRYAG 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 38
Sse9I AATT 1 cut(s) 286
SsiI CCGC 4 cut(s) 221, 228, 230, 291
SspMI CTAG 2 cut(s) 29, 74
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 117
TaqII GACCGA 1 cut(s) 162
TasI AATT 1 cut(s) 286
TauI GCSGC 3 cut(s) 224, 230, 294
TfiI GAWTC 1 cut(s) 86
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 3 cut(s) 178, 194, 346
TseI GCWGC 5 cut(s) 153, 224, 246, 330, 429
Tsp45I GTSAC 3 cut(s) 178, 194, 346
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 183
Tth111I GACNNNGTC 1 cut(s) 184
XceI RCATGY 1 cut(s) 195
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XspI CTAG 2 cut(s) 29, 74
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.