Rh4DG306000

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000301
Physical Location & Seq
Reverse (-)
52781225 .. 52781650
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG306000.1

Sequence Viewer

Length: 426 bp
ATGGCTTTTGCCGTTACTCTCTCTGCAACACTAGTCGAAGAAGAACTATTTTCCAACAAGGAAACCAAGGCAGCAAATGATCACGAAACCTTTGATGCCAAACGCCAAGGGAAAACCTCTTTGAGGGGAACAAGCAGCGTCTTCCTTGGTTCTAGGGAAGTGACGACATCATCATGCGACAAAAACCCCAAGGTTTGTAGAGCCGCCGCAAACAGCCCAAAATCACATTGCTGCCACAACAAGTGTGTGGACGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTGAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGTGCACACAATGTCCGACATGAAACACTGCGGGAGCTGCGACAACCATTGCAAGAGAGGTGGTTCTTGTGCGTTTGGGATGTGCAACTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

141

Amino Acids

15.23

Weight (kDa)

8.62

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 57 - 141 2e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 204, 207, 276, 360
AfiI CCNNNNNNNGG 1 cut(s) 123
AhlI ACTAGT 1 cut(s) 31
AjiI CACGTC 1 cut(s) 253
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw21I GWGCWC 1 cut(s) 336
Alw44I GTGCAC 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 332
ApeKI GCWGC 5 cut(s) 71, 135, 231, 315, 366
AsuHPI GGTGA 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 336
BbsI GAAGAC 2 cut(s) 133, 263
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 5 cut(s) 83, 147, 218, 302, 353
BclI TGATCA 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 2 cut(s) 32, 153
BisI GCNGC 7 cut(s) 72, 136, 204, 207, 232, 316, 367
BlsI GCNGC 7 cut(s) 73, 137, 205, 208, 233, 317, 368
BmgBI CACGTC 1 cut(s) 253
BmsI GCATC 1 cut(s) 85
BpiI GAAGAC 2 cut(s) 133, 263
BsaJI CCNNGG 4 cut(s) 66, 106, 145, 189
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse3DI GCAATG 2 cut(s) 226, 376
BseDI CCNNGG 4 cut(s) 66, 106, 145, 189
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 2 cut(s) 226, 376
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 5 cut(s) 83, 147, 218, 302, 353
BsiHKAI GWGCWC 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 4 cut(s) 204, 207, 276, 360
BsrDI GCAATG 2 cut(s) 226, 376
BssECI CCNNGG 4 cut(s) 66, 106, 145, 189
BssMI GATC 1 cut(s) 79
BssT1I CCWWGG 4 cut(s) 66, 106, 145, 189
Bst4CI ACNGT 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 299, 303
BstDEI CTNAG 1 cut(s) 423
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 414
BstKTI GATC 1 cut(s) 82
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 5 cut(s) 83, 147, 218, 302, 353
BstV2I GAAGAC 2 cut(s) 133, 263
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 299, 303
CseI GACGC 1 cut(s) 127
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 3 cut(s) 174, 326, 349
CviJI RGCY 4 cut(s) 5, 203, 216, 366
CviKI_1 RGCY 4 cut(s) 5, 203, 216, 366
DdeI CTNAG 1 cut(s) 423
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eco130I CCWWGG 4 cut(s) 66, 106, 145, 189
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoT14I CCWWGG 4 cut(s) 66, 106, 145, 189
ErhI CCWWGG 4 cut(s) 66, 106, 145, 189
FaeI CATG 3 cut(s) 177, 329, 352
FaiI YATR 5 cut(s) 175, 313, 327, 350, 420
FatI CATG 3 cut(s) 173, 325, 348
FauI CCCGC 2 cut(s) 269, 353
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 7 cut(s) 72, 136, 204, 207, 232, 316, 367
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 7 cut(s) 72, 136, 204, 207, 232, 316, 367
FspBI CTAG 2 cut(s) 32, 153
GluI GCNGC 7 cut(s) 72, 136, 204, 207, 232, 316, 367
HgaI GACGC 1 cut(s) 127
Hin1II CATG 3 cut(s) 177, 329, 352
HinfI GANTC 1 cut(s) 267
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 250, 334
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 2 cut(s) 250, 334
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 6 cut(s) 26, 297, 318, 334, 381, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 3 cut(s) 177, 329, 352
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 5 cut(s) 83, 147, 218, 302, 353
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 32, 153
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 13, 160
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 4 cut(s) 50, 53, 133, 268
MhlI GDGCHC 1 cut(s) 336
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 2 cut(s) 78, 369
MnlI CCTC 3 cut(s) 117, 127, 380
MslI CAYNNNNRTG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 366
NdeII GATC 1 cut(s) 79
NlaIII CATG 3 cut(s) 177, 329, 352
NmuCI GTSAC 1 cut(s) 160
PkrI GCNGC 7 cut(s) 73, 137, 205, 208, 233, 317, 368
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 172
SatI GCNGC 7 cut(s) 72, 136, 204, 207, 232, 316, 367
Sau3AI GATC 1 cut(s) 79
SchI GAGTC 1 cut(s) 261
SduI GDGCHC 1 cut(s) 336
SetI ASST 6 cut(s) 92, 119, 195, 255, 368, 391
SfaNI GCATC 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 172
SpeI ACTAGT 1 cut(s) 31
SsiI CCGC 4 cut(s) 204, 207, 276, 360
SspMI CTAG 2 cut(s) 32, 153
StyI CCWWGG 4 cut(s) 66, 106, 145, 189
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 36
TauI GCSGC 2 cut(s) 206, 209
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 5 cut(s) 71, 135, 231, 315, 366
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 365
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 1 cut(s) 362
VneI GTGCAC 1 cut(s) 332
XagI CCTNNNNNAGG 1 cut(s) 121
XspI CTAG 2 cut(s) 32, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.