MD07G1060200.v1.1

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000301
Physical Location & Seq
Forward (+)
5594634 .. 5595116
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1060200.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGCCATGAAGTTGATTAACATCTCCTTTACTCTCCTAGCAGCAATGGCCATTGTTGGAGCTGCAAATTTCCAGGACGATCATATCGAAGACGTGGAAATGCAGACTAGTACTGTAGCAGAAGCAACTACATTGCCTGAAACACCAGATGAAGCAGAAGCAACTACTTCTTTAAGAGGAGTGAGCCGTTTTCTTTATAATCATCATAAAAATATCCCACTGCCCAGTTATACTTGCGACAACCTTCCTAGGATTTGTCGTGCGAAGAACAGCCTGGGGCCAGACTGCTGCAAGAAGAAATGTGTTGACGTGAAGACTGATCGGTACAACTGTGGGATTTGCGGCTACAGATGCAAGTATACTGAGATTTGTTGCAGGGGTAAGTGCGTCAATGCATCGTTTGACAAGAGGCATTGTGGTGGGTGCAATCAAAAGTGCAAGAAGGGAGACTTCTGTGTTTATGGGATGTGCCATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

161

Amino Acids

17.79

Weight (kDa)

8.3

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 77 - 160 5.8e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 198
AccI GTMKAC 1 cut(s) 359
AciI CCGC 1 cut(s) 342
AcoI YGGCCR 2 cut(s) 3, 48
AcsI RAATTY 1 cut(s) 67
AfaI GTAC 2 cut(s) 112, 326
AhlI ACTAGT 1 cut(s) 107
AjiI CACGTC 2 cut(s) 94, 310
AjnI CCWGG 2 cut(s) 72, 273
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 441
AoxI GGCC 3 cut(s) 3, 48, 278
ApeKI GCWGC 3 cut(s) 41, 62, 288
ApoI RAATTY 1 cut(s) 67
AspA2I CCTAGG 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 278
AvrII CCTAGG 1 cut(s) 248
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BalI TGGCCA 2 cut(s) 5, 50
BbsI GAAGAC 2 cut(s) 96, 320
BbvI GCAGC 3 cut(s) 49, 53, 275
BceAI ACGGC 1 cut(s) 171
BciT130I CCWGG 2 cut(s) 74, 275
BcoDI GTCTC 1 cut(s) 441
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 38, 108, 249
BfmI CTRYAG 2 cut(s) 114, 346
BisI GCNGC 4 cut(s) 42, 63, 289, 343
BlnI CCTAGG 1 cut(s) 248
BlsI GCNGC 4 cut(s) 43, 64, 290, 344
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 74, 275
BmgBI CACGTC 2 cut(s) 94, 310
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 74, 275
BmrI ACTGGG 1 cut(s) 219
BmsI GCATC 2 cut(s) 341, 404
BmuI ACTGGG 1 cut(s) 219
BpiI GAAGAC 2 cut(s) 96, 320
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 248, 274
Bse1I ACTGG 1 cut(s) 225
Bse3DI GCAATG 2 cut(s) 51, 131
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 2 cut(s) 74, 275
BseDI CCNNGG 2 cut(s) 248, 274
BseGI GGATG 1 cut(s) 471
BseJI GATNNNNATC 1 cut(s) 20
BseMI GCAATG 2 cut(s) 51, 131
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 49, 53, 275
BshFI GGCC 3 cut(s) 5, 50, 280
BsmAI GTCTC 1 cut(s) 441
BsnI GGCC 3 cut(s) 5, 50, 280
Bsp143I GATC 2 cut(s) 79, 319
BspACI CCGC 1 cut(s) 342
BspANI GGCC 3 cut(s) 5, 50, 280
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 279
BsrDI GCAATG 2 cut(s) 51, 131
BsrI ACTGG 1 cut(s) 225
BssECI CCNNGG 2 cut(s) 248, 274
BssMI GATC 2 cut(s) 79, 319
BssNAI GTATAC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 248
Bst1107I GTATAC 1 cut(s) 360
Bst2UI CCWGG 2 cut(s) 74, 275
Bst4CI ACNGT 2 cut(s) 115, 332
BstDEI CTNAG 1 cut(s) 363
BstF5I GGATG 1 cut(s) 471
BstKTI GATC 2 cut(s) 82, 322
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 2 cut(s) 79, 319
BstMWI GCNNNNNNNGC 2 cut(s) 47, 351
BstNI CCWGG 2 cut(s) 74, 275
BstSCI CCNGG 2 cut(s) 72, 273
BstSFI CTRYAG 2 cut(s) 114, 346
BstV1I GCAGC 3 cut(s) 49, 53, 275
BstV2I GAAGAC 2 cut(s) 96, 320
BstZ17I GTATAC 1 cut(s) 360
BsuRI GGCC 3 cut(s) 5, 50, 280
BtrI CACGTC 2 cut(s) 94, 310
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 278
CseI GACGC 1 cut(s) 376
Csp6I GTAC 2 cut(s) 111, 325
CviAII CATG 2 cut(s) 7, 480
CviJI RGCY 7 cut(s) 5, 50, 62, 186, 273, 280, 345
CviKI_1 RGCY 7 cut(s) 5, 50, 62, 186, 273, 280, 345
CviQI GTAC 2 cut(s) 111, 325
DdeI CTNAG 1 cut(s) 363
DpnI GATC 2 cut(s) 81, 321
DpnII GATC 2 cut(s) 79, 319
EaeI YGGCCR 2 cut(s) 3, 48
Eco130I CCWWGG 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 72, 273
EcoT14I CCWWGG 1 cut(s) 248
EcoT22I ATGCAT 2 cut(s) 397, 481
ErhI CCWWGG 1 cut(s) 248
FaeI CATG 2 cut(s) 10, 483
FaiI YATR 9 cut(s) 8, 84, 198, 207, 231, 360, 462, 477, 481
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FatI CATG 2 cut(s) 6, 479
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 4 cut(s) 42, 63, 289, 343
FokI GGATG 1 cut(s) 478
Fsp4HI GCNGC 4 cut(s) 42, 63, 289, 343
FspBI CTAG 3 cut(s) 38, 108, 249
GluI GCNGC 4 cut(s) 42, 63, 289, 343
HaeIII GGCC 3 cut(s) 5, 50, 280
HgaI GACGC 1 cut(s) 376
Hin1II CATG 2 cut(s) 10, 483
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
Hpy166II GTNNAC 2 cut(s) 307, 360
Hpy8I GTNNAC 2 cut(s) 307, 360
HpyAV CCTTC 2 cut(s) 254, 436
HpyCH4III ACNGT 2 cut(s) 115, 332
HpyCH4IV ACGT 2 cut(s) 93, 309
HpyCH4V TGCA 9 cut(s) 65, 103, 291, 354, 375, 395, 426, 438, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 351
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 93, 309
Hsp92II CATG 2 cut(s) 10, 483
Kzo9I GATC 2 cut(s) 79, 319
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 9 cut(s) 59, 86, 150, 159, 238, 260, 287, 294, 361
Lsp1109I GCAGC 3 cut(s) 49, 53, 275
LweI GCATC 2 cut(s) 341, 404
MaeI CTAG 3 cut(s) 38, 108, 249
MaeII ACGT 2 cut(s) 93, 309
MalI GATC 2 cut(s) 81, 321
MboI GATC 2 cut(s) 79, 319
MboII GAAGA 4 cut(s) 101, 277, 307, 325
MlsI TGGCCA 2 cut(s) 5, 50
MluCI AATT 1 cut(s) 67
MluNI TGGCCA 2 cut(s) 5, 50
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 2 cut(s) 170, 402
Mox20I TGGCCA 2 cut(s) 5, 50
Mph1103I ATGCAT 2 cut(s) 397, 481
MscI TGGCCA 2 cut(s) 5, 50
MseI TTAA 2 cut(s) 18, 173
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 2 cut(s) 5, 50
MspR9I CCNGG 2 cut(s) 74, 275
MvaI CCWGG 2 cut(s) 74, 275
MwoI GCNNNNNNNGC 2 cut(s) 47, 351
NdeII GATC 2 cut(s) 79, 319
NlaIII CATG 2 cut(s) 10, 483
NlaIV GGNNCC 1 cut(s) 279
NsiI ATGCAT 2 cut(s) 397, 481
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 4 cut(s) 43, 64, 290, 344
PsiI TTATAA 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 72, 273
PspGI CCWGG 2 cut(s) 72, 273
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 278
RsaI GTAC 2 cut(s) 112, 326
RsaNI GTAC 2 cut(s) 111, 325
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 2 cut(s) 18, 173
SatI GCNGC 4 cut(s) 42, 63, 289, 343
Sau3AI GATC 2 cut(s) 79, 319
Sau96I GGNCC 1 cut(s) 278
ScaI AGTACT 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 74, 275
SetI ASST 4 cut(s) 64, 96, 246, 312
SfaNI GCATC 2 cut(s) 341, 404
SfcI CTRYAG 2 cut(s) 114, 346
SmiMI CAYNNNNRTG 1 cut(s) 417
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 1 cut(s) 67
SsiI CCGC 1 cut(s) 342
SspMI CTAG 3 cut(s) 38, 108, 249
StyD4I CCNGG 2 cut(s) 72, 273
StyI CCWWGG 1 cut(s) 248
TaaI ACNGT 2 cut(s) 115, 332
TaiI ACGT 2 cut(s) 96, 312
TaqI TCGA 1 cut(s) 87
TasI AATT 1 cut(s) 67
TatI WGTACW 1 cut(s) 110
TauI GCSGC 1 cut(s) 345
Tru1I TTAA 2 cut(s) 18, 173
Tru9I TTAA 2 cut(s) 18, 173
TscAI CASTG 1 cut(s) 225
TseI GCWGC 3 cut(s) 41, 62, 288
TspDTI ATGAA 2 cut(s) 23, 165
TspRI CASTG 1 cut(s) 225
XapI RAATTY 1 cut(s) 67
XmaJI CCTAGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 3 cut(s) 38, 108, 249
ZrmI AGTACT 1 cut(s) 112
Zsp2I ATGCAT 2 cut(s) 397, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.