Rorug04G0245200

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000301
Physical Location & Seq
Forward (+)
41517514 .. 41521755
4242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0245200.1

Sequence Viewer

Length: 2136 bp
ATGGCTCTCAGCACATTCTCAATCCCAGCTCACAAACCCACCTCAAACTTCTCATCATCCCATTTCTCTTTTGGACCAGTTCTGCCATGTCCTTACCAACATAAGCTCTTCAATCAGGTAAAGAAAAGGCCAATTGGGATTTGTGCTTCACTGTCAGAGAGTGGAGAGTATCACTCACAAAGACCACCCACTCCTCTCTTGGACACTATCAATTATCCAATTCACATGAAAAACCTCTCTCTCAAGGAACTCAAACAATTAGCTGATGAGCTGAGGTCTGATGTCATATTCAACGTTTCGAAAACCGGAGGTCACCTAGGCTCAAGCCTCGGTGTTGTGGAGCTCACTGTGGCTCTTCACTATGTGTTCAATGCTCCTCAGGACAGGATTCTGTGGGATGTTGGTCATCAGTCTTACCCACACAAAATCTTGACTGGGAGAAGAGATAAGATGCACACTATGAGGCAGACCAATGGGTTAGCTGGTTTCACAAAACGGTCGGAGAGCGAGTATGATTGCTTTGGCACTGGTCACAGTTCCACTACTATCTCAGCTGGCCTGGGAATGGCCGTAGGGAGGGATCTAAAGGGAAAAAACAATCATGTAGTTGCTGTTATAGGTGATGGTGCCATGACAGCAGGGCAAGCTTATGAAGCCATGAACAATGCGGGTTACCTTGATTCCGACATGATAATTATTCTCAATGACAATAAACAGGTTTCTTTACCTACTGCCAATCTCGATGGACCCATCCCTCCAGTAGGAGCTTTGAGTAGTGCTCTCAGTAAGTTGCAATCAAACAGGCCACTTAGAGAATTAAGAGAGGTTGCCAAAGGAGTTACAAAACAAATTGGTGGTTCTGTGCATGAACTGGCAGCAAAAGTTGATGAGTATGCTCGTGGAATGATCAGTGGCTCTGGTTCAACATTGTTTGAAGAGCTTGGACTATATTATATTGGTCCAGTCGATGGTCACAATGTGGATGACCTCATAGCGATACTGCAAGAGGTTAAAACCACGCAAACAACAGGTCCTGTCCTGATCCACCTTATCACTGAGAAAGGCCGGGGATATCCATATGCGGAGAAGGCTGCAGACAAGTACCACGGAGTGGCCAAGTTTGATCCGGCAACCGGAAAGCAGTTCAAAGCCAATGCTAGCACACAGTCTTACACAACATACTTTGCAGAGGCTTTGATTGCAGAAGCTGAAGCGGACAAAGATATTGTAGCAATCCATGCTGCAATGGGAGGTGGAACAGGCATGAATCTCTTCCTCCGCCGTTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCTGTTACTTTTGCCGCAGGTCTTGCCTGTGAAGGCCTGAAACCTTTCTGCGCAATATACTCATCTTTCTTGCAGAGGGCTTATGACCAGGTCGTACATGATGTGGATTTGCAGAAACTGCCTGTGAGATTTGCTATGGATAGAGCTGGGCTTGTTGGGGCTGATGGTCCCACACATTGTGGGGCTTTTGATGTTACTTTTATGGCATGCCTGCCAAACATGGTGGTCATGGCTCCTTCTGATGAGGCAGAGCTTTTCCATATGGTAGCCACTGCTGCTGCCATTGATGACCGCCCAAGTTGTTTCCGGTACCCAAGAGGAAATGGGGTTGGTGTGGAGCTGCCAACAGGAAATAAAGGCACTCCTCTTGAGGTGGGAAAAGGAAGAATATTGATTGAAGGAGAAAGAGTTGCACTCTTGGGCTATGGATCAGCCGTTCAGACATGTTTGGCTGCAGCAACTTTGGTGGAACCTCTTGGCTTGCGTCTGACTGTTGCTGATGCACGATTTTGCAAACCATTGGATCATGCTCTAGTTCGCAAACTTGCAAAATCACATGAGTTTTTGATTACTGTGGAAGAAGGATCCATTGGTGGGTTTGGTTCTCATGTTGTTCAGTTCCTGGCACTTGATGGCCTTCTTGATGGAAAACTGAAGTGGAGACCTCTGGTTCTGCCTGATCGGTACATTGACCATGGTGCCCCGGCTGATCAGTTGGCAGATGCCGGACTCACTCCATGCCATCTAGCAGCAACAGTATTCAACATCCTTGGACAAACAAGAGAGGCATTGGAGGTAATGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

711

Amino Acids

76.54

Weight (kDa)

6.43

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 67 - 352 8.3e-110 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 177 - 238 8.1e-06 Dehydrogenase E1 component
Transket_pyr PF02779 389 - 550 2.3e-50 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 568 - 690 3.4e-28 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1378
Acc36I ACCTGC 1 cut(s) 1334
Acc65I GGTACC 1 cut(s) 1635
AccB1I GGYRCC 3 cut(s) 626, 1635, 2024
AccB7I CCANNNNNTGG 2 cut(s) 968, 1111
AciI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
AclI AACGTT 1 cut(s) 294
AclWI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
AcoI YGGCCR 2 cut(s) 567, 1113
AcuI CTGAAG 2 cut(s) 1230, 2000
AdeI CACNNNGTG 4 cut(s) 364, 979, 1111, 1505
AfaI GTAC 4 cut(s) 1103, 1422, 1637, 2012
AfiI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
AflIII ACRYGT 1 cut(s) 1769
AgsI TTSAA 8 cut(s) 112, 292, 370, 924, 935, 1147, 1724, 2089
AjnI CCWGG 3 cut(s) 558, 1413, 1947
AjuI GAANNNNNNNTTGG 2 cut(s) 1899, 1931
AloI GAACNNNNNNTCC 2 cut(s) 1746, 1778
Alw21I GWGCWC 2 cut(s) 345, 781
Alw26I GTCTC 1 cut(s) 1981
AlwI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
AlwNI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
AoxI GGCC 8 cut(s) 128, 556, 567, 803, 1063, 1113, 1360, 1960
ApeKI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1015, 1047
Asp700I GAANNNNTTC 2 cut(s) 1271, 1370
Asp718I GGTACC 1 cut(s) 1635
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 1 cut(s) 1379
AspS9I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
AsuC2I CCSGG 2 cut(s) 1067, 2030
AsuHPI GGTGA 2 cut(s) 305, 632
AsuII TTCGAA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
AvrII CCTAGG 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 2029
BalI TGGCCA 1 cut(s) 1115
BamHI GGATCC 1 cut(s) 1910
BanI GGYRCC 3 cut(s) 626, 1635, 2024
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 897
Bbv12I GWGCWC 2 cut(s) 345, 781
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BccI CCATC 8 cut(s) 617, 737, 758, 962, 1484, 1952, 1964, 2076
BceAI ACGGC 3 cut(s) 554, 1266, 1745
BciT130I CCWGG 3 cut(s) 560, 1415, 1949
BclI TGATCA 2 cut(s) 906, 2035
BcnI CCSGG 2 cut(s) 1067, 2030
BcoDI GTCTC 1 cut(s) 1981
BfaI CTAG 4 cut(s) 317, 1158, 1859, 2072
BfmI CTRYAG 2 cut(s) 1092, 1779
BfuAI ACCTGC 1 cut(s) 1334
BlnI CCTAGG 1 cut(s) 316
Bme1390I CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
Bme18I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
BmgT120I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
BmiI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BmrFI CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 441, 1286, 1816, 2038
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 6 cut(s) 158, 190, 763, 795, 1725, 1757
BpmI CTGGAG 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 3 cut(s) 227, 307, 1715
BpuMI CCSGG 2 cut(s) 1067, 2030
BsaI GGTCTC 1 cut(s) 1981
BsaJI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BsaWI WCCGGW 3 cut(s) 305, 1133, 1632
BsaXI ACNNNNNCTCC 5 cut(s) 494, 524, 1719, 1749, 2111
Bsc4I CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
Bse1I ACTGG 6 cut(s) 77, 439, 532, 758, 876, 962
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 1251
BseBI CCWGG 3 cut(s) 560, 1415, 1949
BseDI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BseGI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BseLI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
BseMI GCAATG 1 cut(s) 1251
BseMII CTCAG 6 cut(s) 22, 263, 392, 564, 796, 1047
BseNI ACTGG 6 cut(s) 77, 439, 532, 758, 876, 962
BseRI GAGGAG 3 cut(s) 183, 366, 1680
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BseYI CCCAGC 2 cut(s) 25, 1472
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 8 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962
BshNI GGYRCC 3 cut(s) 626, 1635, 2024
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 345, 781
BsiSI CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
BslFI GGGAC 1 cut(s) 1479
BslI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
BsmAI GTCTC 1 cut(s) 1981
BsmFI GGGAC 1 cut(s) 1479
BsnI GGCC 8 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962
Bso31I GGTCTC 1 cut(s) 1981
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 345, 781, 2029
Bsp143I GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
Bsp19I CCATGG 1 cut(s) 2020
BspACI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
BspANI GGCC 8 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962
BspCNI CTCAG 6 cut(s) 21, 264, 391, 563, 795, 1048
BspLI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BspMAI CTGCAG 2 cut(s) 1096, 1783
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
BspQI GCTCTTC 3 cut(s) 113, 360, 930
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 3 cut(s) 626, 1635, 2024
BspTNI GGTCTC 1 cut(s) 1981
BsrDI GCAATG 1 cut(s) 1251
BsrI ACTGG 6 cut(s) 77, 439, 532, 758, 876, 962
BssECI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BssMI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 3 cut(s) 316, 2020, 2095
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 3 cut(s) 560, 1415, 1949
Bst4CI ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
Bst6I CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
BstAPI GCANNNNNTGC 3 cut(s) 1238, 1349, 1444
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
BstDEI CTNAG 7 cut(s) 8, 272, 378, 550, 782, 809, 1056
BstDSI CCRYGG 2 cut(s) 1105, 2020
BstEII GGTNACC 2 cut(s) 311, 671
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 5 cut(s) 56, 403, 750, 988, 2091
BstHHI GCGC 1 cut(s) 1379
BstKTI GATC 9 cut(s) 583, 909, 1044, 1126, 1757, 1852, 1913, 2008, 2038
BstMAI GTCTC 1 cut(s) 1981
BstMBI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
BstNI CCWGG 3 cut(s) 560, 1415, 1949
BstNSI RCATGY 3 cut(s) 1328, 1536, 1773
BstPI GGTNACC 2 cut(s) 311, 671
BstSCI CCNGG 5 cut(s) 558, 1065, 1413, 1947, 2028
BstSFI CTRYAG 2 cut(s) 1092, 1779
BstSLI GKGCMC 1 cut(s) 2029
BstV1I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BstX2I RGATCY 2 cut(s) 580, 1910
BstYI RGATCY 2 cut(s) 580, 1910
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 8 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962
BtgI CCRYGG 2 cut(s) 1105, 2020
BtsCI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 6 cut(s) 149, 345, 525, 916, 1053, 1596
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
CaiI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
CfoI GCGC 1 cut(s) 1379
Cfr13I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
CseI GACGC 1 cut(s) 1799
CsiI ACCWGGT 1 cut(s) 1413
Csp6I GTAC 4 cut(s) 1102, 1421, 1636, 2011
CspCI CAANNNNNGTGG 4 cut(s) 1530, 1565, 1773, 1808
CviQI GTAC 4 cut(s) 1102, 1421, 1636, 2011
DdeI CTNAG 7 cut(s) 8, 272, 378, 550, 782, 809, 1056
DpnI GATC 9 cut(s) 582, 908, 1043, 1125, 1756, 1851, 1912, 2007, 2037
DpnII GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
DraIII CACNNNGTG 4 cut(s) 364, 979, 1111, 1505
EaeI YGGCCR 2 cut(s) 567, 1113
Eam1104I CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
EarI CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
EciI GGCGGA 1 cut(s) 1268
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 316, 2020, 2095
Eco147I AGGCCT 1 cut(s) 1362
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 1981
Eco32I GATATC 1 cut(s) 1073
Eco47I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 1230, 2000
Eco81I CCTNAGG 1 cut(s) 378
Eco91I GGTNACC 2 cut(s) 311, 671
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 1031
EcoO65I GGTNACC 2 cut(s) 311, 671
EcoRII CCWGG 3 cut(s) 558, 1413, 1947
EcoRV GATATC 1 cut(s) 1073
EcoT14I CCWWGG 3 cut(s) 316, 2020, 2095
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 316, 2020, 2095
FaqI GGGAC 1 cut(s) 1479
FauI CCCGC 1 cut(s) 661
FauNDI CATATG 2 cut(s) 1078, 1587
FbaI TGATCA 2 cut(s) 906, 2035
FokI GGATG 5 cut(s) 43, 410, 737, 995, 2078
FriOI GRGCYC 1 cut(s) 345
FspBI CTAG 4 cut(s) 317, 1158, 1859, 2072
FspI TGCGCA 1 cut(s) 1378
GlaI GCGC 1 cut(s) 1378
GsaI CCCAGC 2 cut(s) 29, 1476
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 8 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962
HapII CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 1 cut(s) 1379
Hin6I GCGC 1 cut(s) 1377
HinP1I GCGC 1 cut(s) 1377
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 4 cut(s) 388, 680, 1267, 2055
HpaII CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
HphI GGTGA 2 cut(s) 305, 632
Hpy188I TCNGA 8 cut(s) 157, 280, 502, 685, 1567, 1767, 1815, 2135
Hpy188III TCNNGA 6 cut(s) 380, 430, 740, 1039, 1694, 1967
HpyAV CCTTC 6 cut(s) 1081, 1352, 1572, 1718, 1901, 1973
HpyCH4III ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
HpyCH4IV ACGT 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
HpyF3I CTNAG 7 cut(s) 8, 272, 378, 550, 782, 809, 1056
HpySE526I ACGT 1 cut(s) 294
HspAI GCGC 1 cut(s) 1377
KpnI GGTACC 1 cut(s) 1639
Ksp22I TGATCA 2 cut(s) 906, 2035
Kzo9I GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
LguI GCTCTTC 3 cut(s) 113, 360, 930
LmnI GCTCC 5 cut(s) 340, 379, 764, 1564, 1663
Lsp1109I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
LweI GCATC 4 cut(s) 441, 1286, 1816, 2038
MabI ACCWGGT 1 cut(s) 1413
MaeI CTAG 4 cut(s) 317, 1158, 1859, 2072
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 7 cut(s) 311, 530, 671, 838, 971, 1330, 1519
MalI GATC 9 cut(s) 582, 908, 1043, 1125, 1756, 1851, 1912, 2007, 2037
MboI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
MboII GAAGA 7 cut(s) 100, 347, 453, 947, 1264, 1722, 1916
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 2 cut(s) 580, 1910
MhlI GDGCHC 3 cut(s) 345, 781, 2029
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 7 cut(s) 132, 211, 219, 257, 693, 815, 849
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 1 cut(s) 2049
MmeI TCCRAC 4 cut(s) 480, 708, 1288, 1313
Mox20I TGGCCA 1 cut(s) 1115
MroXI GAANNNNTTC 2 cut(s) 1271, 1370
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 2 cut(s) 818, 1011
Msp20I TGGCCA 1 cut(s) 1115
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
MspR9I CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 3 cut(s) 560, 1415, 1949
MwoI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
NciI CCSGG 2 cut(s) 1067, 2030
NcoI CCATGG 1 cut(s) 2020
NdeI CATATG 2 cut(s) 1078, 1587
NdeII GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
NmuCI GTSAC 3 cut(s) 311, 530, 971
NsbI TGCGCA 1 cut(s) 1378
NspI RCATGY 3 cut(s) 1328, 1536, 1773
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 2 cut(s) 1328, 1536
PceI AGGCCT 1 cut(s) 1362
PciI ACATGT 1 cut(s) 1769
PciSI GCTCTTC 3 cut(s) 113, 360, 930
PdmI GAANNNNTTC 2 cut(s) 1271, 1370
PfeI GAWTC 3 cut(s) 388, 680, 1267
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 2 cut(s) 968, 1111
PleI GAGTC 1 cut(s) 2049
PpsI GAGTC 1 cut(s) 2049
PpuMI RGGWCCY 1 cut(s) 1031
PscI ACATGT 1 cut(s) 1769
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 3 cut(s) 558, 1413, 1947
PspEI GGTNACC 2 cut(s) 311, 671
PspFI CCCAGC 2 cut(s) 25, 1472
PspGI CCWGG 3 cut(s) 558, 1413, 1947
PspN4I GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
PspPI GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
PspPPI RGGWCCY 1 cut(s) 1031
PstI CTGCAG 2 cut(s) 1096, 1783
PstNI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
PsuI RGATCY 2 cut(s) 580, 1910
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 1103, 1422, 1637, 2012
RsaNI GTAC 4 cut(s) 1102, 1421, 1636, 2011
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 3 cut(s) 113, 360, 930
SaqAI TTAA 2 cut(s) 818, 1011
Sau3AI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
Sau96I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
SchI GAGTC 1 cut(s) 2049
ScrFI CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
SduI GDGCHC 3 cut(s) 345, 781, 2029
SexAI ACCWGGT 1 cut(s) 1413
SfaNI GCATC 4 cut(s) 441, 1286, 1816, 2038
SfcI CTRYAG 2 cut(s) 1092, 1779
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
SmlI CTYRAG 3 cut(s) 242, 322, 1694
SmoI CTYRAG 3 cut(s) 242, 322, 1694
SphI GCATGC 2 cut(s) 1328, 1536
Sse9I AATT 7 cut(s) 132, 211, 219, 257, 693, 815, 849
SseBI AGGCCT 1 cut(s) 1362
SsiI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
SspI AATATT 1 cut(s) 1716
SspMI CTAG 4 cut(s) 317, 1158, 1859, 2072
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 1 cut(s) 1362
StyD4I CCNGG 5 cut(s) 558, 1065, 1413, 1947, 2028
StyI CCWWGG 3 cut(s) 316, 2020, 2095
TaaI ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 299, 741, 966
TasI AATT 7 cut(s) 132, 211, 219, 257, 693, 815, 849
TauI GCSGC 1 cut(s) 1343
TfiI GAWTC 3 cut(s) 388, 680, 1267
Tru1I TTAA 2 cut(s) 818, 1011
Tru9I TTAA 2 cut(s) 818, 1011
TscAI CASTG 6 cut(s) 156, 352, 532, 916, 1060, 1603
TseFI GTSAC 3 cut(s) 311, 530, 971
TseI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
Tsp45I GTSAC 3 cut(s) 311, 530, 971
TspDTI ATGAA 5 cut(s) 242, 666, 674, 882, 1280
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 6 cut(s) 156, 352, 532, 916, 1060, 1603
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 2 cut(s) 968, 1111
VpaK11BI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
XagI CCTNNNNNAGG 1 cut(s) 759
XceI RCATGY 3 cut(s) 1328, 1536, 1773
XcmI CCANNNNNNNNNTGG 2 cut(s) 68, 196
XmaJI CCTAGG 1 cut(s) 316
XmnI GAANNNNTTC 2 cut(s) 1271, 1370
XspI CTAG 4 cut(s) 317, 1158, 1859, 2072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.