RLG00000031035

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000301
Physical Location & Seq
Forward (+)
1502212 .. 1502652
441 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031035

Sequence Viewer

Length: 441 bp
ATGCTAATGGCTTTGGCCGCCATTACTATTTCCGCATCAACCCCGGAAGAAGATCAACAGAAATGGTTCTCCGATGAGGAAAATGATACTCCAAATGAACCATCAGATGATCTCCCTGCGACCTTAAGCCAACCAAAAACTTCCCTTAGGGGAGCAAGCCGCTTCCTTGCCACCCGGGCTGTTGCGACTACCTGTGACAAAAACCCTAAGGTTTGTAAGGCTGCAGGCAGTGCGGGGCAAGATTGCTGCAAGAAGAAGTGTGTAAATTTGAAGACAGACAGAGTCAATTGCGGCAAGTGTGGGAAGAAATGCAAGTACTCGGAGATATGCTGCAAAGGAAAGTGTCTGAATCCAATGTCTGACAAGAAAAACTGTGGGAGCTGCAACAACAAGTGCAAGAAAGGCAGTTTGTGTGTGTACGGAATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

147

Amino Acids

15.71

Weight (kDa)

8.84

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 63 - 146 1.7e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 18, 33, 160, 233, 291
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 265
AfaI GTAC 2 cut(s) 317, 419
AflII CTTAAG 1 cut(s) 124
AgsI TTSAA 1 cut(s) 271
AluBI AGCT 2 cut(s) 381, 432
AluI AGCT 2 cut(s) 381, 432
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 5 cut(s) 221, 246, 330, 381, 429
ApoI RAATTY 1 cut(s) 265
Asp700I GAANNNNTTC 1 cut(s) 65
AsuC2I CCSGG 3 cut(s) 44, 175, 176
AvaI CYCGRG 1 cut(s) 174
AxyI CCTNAGG 2 cut(s) 146, 207
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 208, 233, 317, 368
BccI CCATC 1 cut(s) 109
BcnI CCSGG 3 cut(s) 44, 175, 176
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 8 cut(s) 18, 160, 222, 247, 292, 331, 382, 430
BlsI GCNGC 8 cut(s) 19, 161, 223, 248, 293, 332, 383, 431
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 3 cut(s) 44, 175, 176
BmeT110I CYCGRG 1 cut(s) 174
BmrFI CCNGG 3 cut(s) 44, 175, 176
BmsI GCATC 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 278
BpuMI CCSGG 3 cut(s) 44, 175, 176
BsaJI CCNNGG 2 cut(s) 42, 174
Bse21I CCTNAGG 2 cut(s) 146, 207
BseDI CCNNGG 2 cut(s) 42, 174
BseXI GCAGC 4 cut(s) 208, 233, 317, 368
BshFI GGCC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 2 cut(s) 44, 175
BsnI GGCC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 174
Bsp143I GATC 2 cut(s) 52, 109
BspACI CCGC 5 cut(s) 18, 33, 160, 233, 291
BspANI GGCC 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 226
BspTI CTTAAG 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 42, 174
BssMI GATC 2 cut(s) 52, 109
Bst4CI ACNGT 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 157, 226
BstDEI CTNAG 2 cut(s) 146, 207
BstKTI GATC 2 cut(s) 55, 112
BstMBI GATC 2 cut(s) 52, 109
BstMWI GCNNNNNNNGC 4 cut(s) 17, 176, 230, 402
BstSCI CCNGG 3 cut(s) 42, 173, 174
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 208, 233, 317, 368
BstV2I GAAGAC 1 cut(s) 278
Bsu36I CCTNAGG 2 cut(s) 146, 207
BsuRI GGCC 1 cut(s) 17
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 157, 226
Cfr9I CCCGGG 1 cut(s) 174
Csp6I GTAC 2 cut(s) 316, 418
CviJI RGCY 8 cut(s) 11, 17, 129, 159, 179, 221, 381, 432
CviKI_1 RGCY 8 cut(s) 11, 17, 129, 159, 179, 221, 381, 432
CviQI GTAC 2 cut(s) 316, 418
DdeI CTNAG 2 cut(s) 146, 207
DpnI GATC 2 cut(s) 54, 111
DpnII GATC 2 cut(s) 52, 109
EaeI YGGCCR 1 cut(s) 15
Eco81I CCTNAGG 2 cut(s) 146, 207
Eco88I CYCGRG 1 cut(s) 174
EcoT22I ATGCAT 1 cut(s) 439
FaiI YATR 3 cut(s) 328, 435, 439
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 8 cut(s) 18, 160, 222, 247, 292, 331, 382, 430
Fsp4HI GCNGC 8 cut(s) 18, 160, 222, 247, 292, 331, 382, 430
GluI GCNGC 8 cut(s) 18, 160, 222, 247, 292, 331, 382, 430
HaeIII GGCC 1 cut(s) 17
HapII CCGG 2 cut(s) 44, 175
HinfI GANTC 2 cut(s) 282, 349
HpaII CCGG 2 cut(s) 44, 175
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 5 cut(s) 73, 106, 322, 348, 361
Hpy8I GTNNAC 1 cut(s) 418
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4V TGCA 8 cut(s) 224, 249, 312, 333, 384, 396, 429, 437
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 176, 230, 402
HpyF3I CTNAG 2 cut(s) 146, 207
Kzo9I GATC 2 cut(s) 52, 109
LmnI GCTCC 2 cut(s) 152, 378
LpnPI CCDG 5 cut(s) 57, 129, 188, 205, 210
Lsp1109I GCAGC 4 cut(s) 208, 233, 317, 368
LweI GCATC 1 cut(s) 44
MaeIII GTNAC 1 cut(s) 194
MalI GATC 2 cut(s) 54, 111
MboI GATC 2 cut(s) 52, 109
MboII GAAGA 5 cut(s) 59, 62, 265, 283, 316
MfeI CAATTG 1 cut(s) 286
MluCI AATT 2 cut(s) 265, 286
MlyI GAGTC 1 cut(s) 291
MnlI CCTC 1 cut(s) 70
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 65
MseI TTAA 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 124
MspI CCGG 2 cut(s) 44, 175
MspR9I CCNGG 3 cut(s) 44, 175, 176
MunI CAATTG 1 cut(s) 286
MwoI GCNNNNNNNGC 4 cut(s) 17, 176, 230, 402
NciI CCSGG 3 cut(s) 44, 175, 176
NdeII GATC 2 cut(s) 52, 109
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 349
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 8 cut(s) 19, 161, 223, 248, 293, 332, 383, 431
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PstI CTGCAG 1 cut(s) 226
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 2 cut(s) 317, 419
RsaNI GTAC 2 cut(s) 316, 418
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 8 cut(s) 18, 160, 222, 247, 292, 331, 382, 430
Sau3AI GATC 2 cut(s) 52, 109
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 3 cut(s) 44, 175, 176
SetI ASST 5 cut(s) 125, 194, 213, 383, 434
SfaNI GCATC 1 cut(s) 44
SfcI CTRYAG 1 cut(s) 222
SmaI CCCGGG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 2 cut(s) 265, 286
SsiI CCGC 5 cut(s) 18, 33, 160, 233, 291
StyD4I CCNGG 3 cut(s) 42, 173, 174
TaaI ACNGT 1 cut(s) 374
TasI AATT 2 cut(s) 265, 286
TatI WGTACW 1 cut(s) 315
TauI GCSGC 3 cut(s) 20, 162, 294
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 5 cut(s) 221, 246, 330, 381, 429
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 1 cut(s) 111
TspGWI ACGGA 1 cut(s) 435
TspMI CCCGGG 1 cut(s) 174
TspRI CASTG 1 cut(s) 235
Tth111I GACNNNGTC 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 124
XapI RAATTY 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 65
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.