MD11G1212900.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000301
Physical Location & Seq
Forward (+)
31097599 .. 31098063
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1212900.v1.1.491

Sequence Viewer

Length: 465 bp
ATGAAGTTGTATATACATTGTTTCTTCATATCTATCCTCCTCTTGTCCCATGTTCTAAGCGTTGAATCCAATGAAGTACAATCCCAACAATACTACGAAGTTGCAACCAATGATGAGTTTTCAAACTCTCTGGTAAACAAAACTGAATCACAACCTTCGATTATGTTACATGGAAACGACCGCTTTCTCTTGCAAAAACACCGGGTGCGAATGACTTGTAACAAGTTCCCCGGGATATGTCATGCTAAAGGAAGCCTTGGGCCTTATTGTTGCAAGAAGAAGTGTGTTAATGTGTTTACTGACCGAGCTAATTGTGGGAGGTGTGGGAAGAAGTGCAAGTATAATGAGATATGCTGCAAAGCGAAATGTGTGAACCCATCTTTCCATAGAAGGCATTGTGGTGGCTGCAACAATAGGTGCAAAGATGGAGGGTTTTGTGCATTTGGCCTATGCAACTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

155

Amino Acids

17.45

Weight (kDa)

9.05

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 71 - 154 2.6e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 181
AdeI CACNNNGTG 1 cut(s) 205
AfaI GTAC 1 cut(s) 78
AgsI TTSAA 2 cut(s) 65, 123
AluBI AGCT 1 cut(s) 308
AluI AGCT 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 260, 445
ApeKI GCWGC 2 cut(s) 354, 405
AspS9I GGNCC 1 cut(s) 260
AsuC2I CCSGG 3 cut(s) 203, 231, 232
AvaI CYCGRG 1 cut(s) 230
BbvI GCAGC 2 cut(s) 341, 392
BccI CCATC 2 cut(s) 385, 419
BcnI CCSGG 3 cut(s) 203, 231, 232
BisI GCNGC 2 cut(s) 355, 406
BlsI GCNGC 2 cut(s) 356, 407
Bme1390I CCNGG 3 cut(s) 203, 231, 232
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 260
BmrFI CCNGG 3 cut(s) 203, 231, 232
BpuMI CCSGG 3 cut(s) 203, 231, 232
BsaJI CCNNGG 3 cut(s) 229, 230, 256
BseDI CCNNGG 3 cut(s) 229, 230, 256
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 2 cut(s) 341, 392
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 2 cut(s) 262, 447
BsiEI CGRYCG 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 202, 231
BslFI GGGAC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 2 cut(s) 262, 447
BsoBI CYCGRG 1 cut(s) 230
BspACI CCGC 1 cut(s) 181
BspANI GGCC 2 cut(s) 262, 447
BssECI CCNNGG 3 cut(s) 229, 230, 256
BssT1I CCWWGG 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 56
BstMCI CGRYCG 1 cut(s) 181
BstSCI CCNGG 3 cut(s) 201, 229, 230
BstV1I GCAGC 2 cut(s) 341, 392
BsuRI GGCC 2 cut(s) 262, 447
Cfr13I GGNCC 1 cut(s) 260
Cfr9I CCCGGG 1 cut(s) 230
Csp6I GTAC 1 cut(s) 77
CviAII CATG 3 cut(s) 50, 170, 242
CviJI RGCY 5 cut(s) 255, 262, 308, 405, 447
CviKI_1 RGCY 5 cut(s) 255, 262, 308, 405, 447
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 1 cut(s) 56
DraIII CACNNNGTG 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 256
Eco88I CYCGRG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 463
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 3 cut(s) 53, 173, 245
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 31
FatI CATG 3 cut(s) 49, 169, 241
Fnu4HI GCNGC 2 cut(s) 355, 406
Fsp4HI GCNGC 2 cut(s) 355, 406
GluI GCNGC 2 cut(s) 355, 406
HaeIII GGCC 2 cut(s) 262, 447
HapII CCGG 2 cut(s) 202, 231
Hin1II CATG 3 cut(s) 53, 173, 245
HinfI GANTC 2 cut(s) 65, 146
HpaII CCGG 2 cut(s) 202, 231
Hpy166II GTNNAC 3 cut(s) 136, 297, 373
Hpy8I GTNNAC 3 cut(s) 136, 297, 373
HpyAV CCTTC 2 cut(s) 165, 384
HpyF3I CTNAG 1 cut(s) 56
Hsp92II CATG 3 cut(s) 53, 173, 245
LpnPI CCDG 3 cut(s) 116, 215, 244
Lsp1109I GCAGC 2 cut(s) 341, 392
MaeIII GTNAC 2 cut(s) 165, 218
MboII GAAGA 3 cut(s) 16, 289, 340
MluCI AATT 1 cut(s) 310
MnlI CCTC 4 cut(s) 47, 50, 312, 422
Mph1103I ATGCAT 1 cut(s) 463
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 399
MspI CCGG 2 cut(s) 202, 231
MspR9I CCNGG 3 cut(s) 203, 231, 232
NciI CCSGG 3 cut(s) 203, 231, 232
NlaIII CATG 3 cut(s) 53, 173, 245
NsiI ATGCAT 1 cut(s) 463
PfeI GAWTC 2 cut(s) 65, 146
PkrI GCNGC 2 cut(s) 356, 407
PspPI GGNCC 1 cut(s) 260
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 399
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 2 cut(s) 355, 406
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 203, 231, 232
SetI ASST 4 cut(s) 157, 310, 323, 419
SmaI CCCGGG 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 399
Sse9I AATT 1 cut(s) 310
SsiI CCGC 1 cut(s) 181
StyD4I CCNGG 3 cut(s) 201, 229, 230
StyI CCWWGG 1 cut(s) 256
TaqI TCGA 1 cut(s) 158
TaqII GACCGA 1 cut(s) 318
TasI AATT 1 cut(s) 310
TatI WGTACW 1 cut(s) 76
TfiI GAWTC 2 cut(s) 65, 146
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseI GCWGC 2 cut(s) 354, 405
TspDTI ATGAA 3 cut(s) 16, 17, 87
TspMI CCCGGG 1 cut(s) 230
XmaI CCCGGG 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.