Rh5DG020400

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000301
Physical Location & Seq
Forward (+)
1502436 .. 1503345
910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG020400.1

Sequence Viewer

Length: 267 bp
ATGAAAGGCTCCATCCAAGTCTTCTTTTTGCTAGCCATGCTAATGGCTTTAGCCGCCATTACTCTTTCCGCATCAACCCCGGAGGAAGATCGAGATGAATGGTTCTCCGATGAGGAAACAGATACTCCAAATGAACCATCTGATGATCTCCCTACTAGTATGGATGACTTGGAACAAGCCTATGAGATGCAGATTCAACCTGATCCTTTGCAATTAAAGTTACCAAAGAGAGGCTTTCACCAACAGAGGCACCTAGTGCAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

88

Amino Acids

10.08

Weight (kDa)

4.2

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 249
AciI CCGC 2 cut(s) 54, 69
AclWI GGATC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 1 cut(s) 197
AhlI ACTAGT 1 cut(s) 155
AlwI GGATC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 230
AsuNHI GCTAGC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 13
BccI CCATC 2 cut(s) 20, 145
BcnI CCSGG 1 cut(s) 80
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 3 cut(s) 32, 156, 254
BisI GCNGC 2 cut(s) 54, 260
BlsI GCNGC 2 cut(s) 55, 261
Bme1390I CCNGG 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 10, 251
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 80, 177
BmtI GCTAGC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 12, 169
BseLI CCNNNNNNNGG 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 230
Bsp143I GATC 3 cut(s) 88, 145, 202
BspACI CCGC 2 cut(s) 54, 69
BspLI GGNNCC 2 cut(s) 10, 251
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 88, 145, 202
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 33
BstF5I GGATG 2 cut(s) 12, 169
BstKTI GATC 3 cut(s) 91, 148, 205
BstMBI GATC 3 cut(s) 88, 145, 202
BstMWI GCNNNNNNNGC 3 cut(s) 37, 53, 256
BstSCI CCNGG 1 cut(s) 78
BstV2I GAAGAC 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 12, 169
Cac8I GCNNGC 1 cut(s) 33
CviAII CATG 1 cut(s) 37
CviJI RGCY 7 cut(s) 9, 35, 47, 53, 179, 234, 262
CviKI_1 RGCY 7 cut(s) 9, 35, 47, 53, 179, 234, 262
DpnI GATC 3 cut(s) 90, 147, 204
DpnII GATC 3 cut(s) 88, 145, 202
DraIII CACNNNGTG 1 cut(s) 256
FaeI CATG 1 cut(s) 40
FaiI YATR 3 cut(s) 38, 161, 183
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 54, 260
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 54, 260
FspBI CTAG 3 cut(s) 32, 156, 254
GluI GCNGC 2 cut(s) 54, 260
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 40
HinfI GANTC 1 cut(s) 193
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 230
Hpy188I TCNGA 2 cut(s) 109, 142
Hpy188III TCNNGA 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 190, 211, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 53, 256
Hsp92II CATG 1 cut(s) 40
Kzo9I GATC 3 cut(s) 88, 145, 202
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 2 cut(s) 93, 213
LweI GCATC 2 cut(s) 80, 177
MaeI CTAG 3 cut(s) 32, 156, 254
MaeIII GTNAC 1 cut(s) 219
MalI GATC 3 cut(s) 90, 147, 204
MboI GATC 3 cut(s) 88, 145, 202
MboII GAAGA 2 cut(s) 13, 98
MluCI AATT 1 cut(s) 212
MnlI CCTC 4 cut(s) 76, 106, 224, 240
MseI TTAA 2 cut(s) 215, 265
MslI CAYNNNNRTG 1 cut(s) 41
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 37, 53, 256
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 88, 145, 202
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 10, 251
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 2 cut(s) 55, 261
PspN4I GGNNCC 2 cut(s) 10, 251
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 2 cut(s) 215, 265
SatI GCNGC 2 cut(s) 54, 260
Sau3AI GATC 3 cut(s) 88, 145, 202
ScrFI CCNGG 1 cut(s) 80
SetI ASST 2 cut(s) 202, 255
SfaNI GCATC 2 cut(s) 80, 177
SmiMI CAYNNNNRTG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 1 cut(s) 212
SsiI CCGC 2 cut(s) 54, 69
SspMI CTAG 3 cut(s) 32, 156, 254
StyD4I CCNGG 1 cut(s) 78
TaqI TCGA 1 cut(s) 91
TasI AATT 1 cut(s) 212
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 2 cut(s) 215, 265
Tru9I TTAA 2 cut(s) 215, 265
TseI GCWGC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 17, 111, 147
XspI CTAG 3 cut(s) 32, 156, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.