Rh5DG020300

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000301
Physical Location & Seq
Forward (+)
1493075 .. 1498408
5334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG020300.1

Sequence Viewer

Length: 789 bp
ATGGAAATGATAGTATCCAAAATTGTGTTCACCCTAATCTTCGTGTTGTTGGGTATAGCCATTGCCCATTCAGCAACACCAACTGTCGAAGAGGAGCCCCAACTCAACCTTAACGAGCTTTCGTCCGCAATGGCATCTCTGCGAGGGATTGGTGGCCGGTTCCTTGCGGAGCAAGTACGAGACGCACCGACCACATGTGACAAGAATCCGACAGTCTGTAAGAGCAAGGGCAGTGCAGGGCCTAACTGCTGCAGCAAAAATTGCGTCAATGTAACGACGGATACAAACAATTGCGGGCGGTGTGGAGTGAAGTGTAAATACTCGGAACTCTGCTGCAACGGTGTTTGTGTGAACCCGTCTGTCAATGGCAAACATTGTGGCAAGTGCGGCAACAAGTGTGGAAAAGGAAGCTCATGCTTGGTTACAGAACAGAAGGGTGTGAGATTACATGTACTTACGAATAAGGCCAAAGTGGCCACACTCCGGTGGATATTTGCCATTGGAAACATTCTGTACCAGTTTCCATCTCTACGGCGCATAGCTTCTAGCAAGATAAGAATATGTACAGAGTTTGTGGAAGAGCTGATAATTTTTTTAAGGAGAAATGGCAATTCAGAAACACTAGTATTGCTAAGAGAGCAATGGTTGTTGGACTTGGTGAACTGCCAGGTCTTCTTCCATGGATACTTAGTTCTCATGATTCTCAACCATGCATTCAGTGGCATTGCTGTGTCTATGGTAATGAAGTATGCTAACAATATTGTGAAGAATAAGGCTTACGTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

262

Amino Acids

28.76

Weight (kDa)

9.1

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 64 - 140 4.9e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 126, 167, 294, 298, 387
AcoI YGGCCR 2 cut(s) 154, 474
AfaI GTAC 4 cut(s) 177, 453, 515, 565
AfiI CCNNNNNNNGG 1 cut(s) 483
AflIII ACRYGT 2 cut(s) 194, 448
AhlI ACTAGT 1 cut(s) 622
AjnI CCWGG 1 cut(s) 666
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AleI CACNNNNGTG 1 cut(s) 484
AloI GAACNNNNNNTCC 2 cut(s) 675, 707
AluBI AGCT 4 cut(s) 118, 411, 542, 583
AluI AGCT 4 cut(s) 118, 411, 542, 583
Alw26I GTCTC 1 cut(s) 174
AoxI GGCC 4 cut(s) 154, 239, 465, 474
ApeKI GCWGC 3 cut(s) 249, 252, 333
ArsI GACNNNNNNTTYG 2 cut(s) 249, 281
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 22, 670
BalI TGGCCA 1 cut(s) 476
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
BbsI GAAGAC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 236, 264, 320
BccI CCATC 1 cut(s) 532
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 668
BciVI GTATCC 3 cut(s) 25, 274, 677
BcoDI GTCTC 1 cut(s) 174
BcuI ACTAGT 1 cut(s) 622
BfaI CTAG 2 cut(s) 546, 623
BfmI CTRYAG 1 cut(s) 250
BfuI GTATCC 3 cut(s) 25, 274, 677
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 4 cut(s) 250, 253, 334, 388
BlsI GCNGC 4 cut(s) 251, 254, 335, 389
Bme1390I CCNGG 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 96, 161
BmrFI CCNGG 1 cut(s) 668
BmsI GCATC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 664
BsaAI YACGTR 1 cut(s) 781
BsaJI CCNNGG 1 cut(s) 679
BsaWI WCCGGW 1 cut(s) 483
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 4 cut(s) 60, 135, 647, 723
BseBI CCWGG 1 cut(s) 668
BseDI CCNNGG 1 cut(s) 679
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 4 cut(s) 60, 135, 647, 723
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 3 cut(s) 236, 264, 320
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 4 cut(s) 156, 241, 467, 476
BsiSI CCGG 2 cut(s) 157, 484
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 4 cut(s) 156, 241, 467, 476
Bsp1286I GDGCHC 1 cut(s) 99
Bsp1407I TGTACA 1 cut(s) 563
Bsp19I CCATGG 1 cut(s) 679
BspACI CCGC 5 cut(s) 126, 167, 294, 298, 387
BspANI GGCC 4 cut(s) 156, 241, 467, 476
BspHI TCATGA 1 cut(s) 696
BspLI GGNNCC 2 cut(s) 96, 161
BspMAI CTGCAG 1 cut(s) 254
BspQI GCTCTTC 1 cut(s) 573
BsrDI GCAATG 4 cut(s) 60, 135, 647, 723
BsrFI RCCGGY 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 563
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 679
BssT1I CCWWGG 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 85, 214, 341
Bst6I CTCTTC 2 cut(s) 84, 573
BstAPI GCANNNNNTGC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 781
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 2 cut(s) 632, 688
BstDSI CCRYGG 1 cut(s) 679
BstHHI GCGC 1 cut(s) 537
BstMAI GTCTC 1 cut(s) 174
BstMWI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 473, 637
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 2 cut(s) 198, 452
BstSCI CCNGG 1 cut(s) 666
BstSFI CTRYAG 1 cut(s) 250
BstSNI TACGTA 1 cut(s) 781
BstV1I GCAGC 3 cut(s) 236, 264, 320
BstV2I GAAGAC 1 cut(s) 664
BsuI GTATCC 3 cut(s) 25, 274, 677
BsuRI GGCC 4 cut(s) 156, 241, 467, 476
BtgI CCRYGG 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 724
Cac8I GCNNGC 1 cut(s) 296
CciI TCATGA 1 cut(s) 696
CfoI GCGC 1 cut(s) 537
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 2 cut(s) 191, 253
Csp6I GTAC 4 cut(s) 176, 452, 514, 564
CspCI CAANNNNNGTGG 4 cut(s) 358, 379, 393, 414
CviAII CATG 6 cut(s) 195, 414, 449, 680, 697, 710
CviQI GTAC 4 cut(s) 176, 452, 514, 564
DdeI CTNAG 2 cut(s) 632, 688
EaeI YGGCCR 2 cut(s) 154, 474
Eam1104I CTCTTC 2 cut(s) 84, 573
EarI CTCTTC 2 cut(s) 84, 573
Eco105I TACGTA 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 679
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 679
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 198, 417, 452, 683, 700, 713
FatI CATG 6 cut(s) 194, 413, 448, 679, 696, 709
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 4 cut(s) 250, 253, 334, 388
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 250, 253, 334, 388
FspBI CTAG 2 cut(s) 546, 623
GlaI GCGC 1 cut(s) 536
GluI GCNGC 4 cut(s) 250, 253, 334, 388
HaeIII GGCC 4 cut(s) 156, 241, 467, 476
HapII CCGG 2 cut(s) 157, 484
HgaI GACGC 2 cut(s) 191, 253
HhaI GCGC 1 cut(s) 537
Hin1II CATG 6 cut(s) 198, 417, 452, 683, 700, 713
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 2 cut(s) 205, 700
HpaII CCGG 2 cut(s) 157, 484
HphI GGTGA 2 cut(s) 22, 670
Hpy166II GTNNAC 3 cut(s) 30, 352, 661
Hpy188I TCNGA 3 cut(s) 210, 325, 616
Hpy188III TCNNGA 1 cut(s) 697
Hpy8I GTNNAC 3 cut(s) 30, 352, 661
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 3 cut(s) 85, 214, 341
HpyCH4IV ACGT 1 cut(s) 780
HpyCH4V TGCA 4 cut(s) 236, 252, 336, 713
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 473, 637
HpyF3I CTNAG 2 cut(s) 632, 688
HpySE526I ACGT 1 cut(s) 780
Hsp92II CATG 6 cut(s) 198, 417, 452, 683, 700, 713
HspAI GCGC 1 cut(s) 535
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 94, 169
LpnPI CCDG 6 cut(s) 170, 222, 497, 530, 653, 680
Lsp1109I GCAGC 3 cut(s) 236, 264, 320
LweI GCATC 1 cut(s) 143
MaeI CTAG 2 cut(s) 546, 623
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 3 cut(s) 197, 271, 421
MboII GAAGA 6 cut(s) 31, 101, 590, 664, 667, 778
MfeI CAATTG 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 5 cut(s) 21, 259, 289, 588, 610
MluNI TGGCCA 1 cut(s) 476
MmeI TCCRAC 2 cut(s) 233, 630
MnlI CCTC 2 cut(s) 85, 137
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 1 cut(s) 715
MscI TGGCCA 1 cut(s) 476
MseI TTAA 2 cut(s) 111, 596
MslI CAYNNNNRTG 2 cut(s) 484, 728
Msp20I TGGCCA 1 cut(s) 476
MspI CCGG 2 cut(s) 157, 484
MspR9I CCNGG 1 cut(s) 668
MunI CAATTG 1 cut(s) 289
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 1 cut(s) 668
MwoI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 473, 637
NcoI CCATGG 1 cut(s) 679
NlaIII CATG 6 cut(s) 198, 417, 452, 683, 700, 713
NlaIV GGNNCC 2 cut(s) 96, 161
NmuCI GTSAC 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 2 cut(s) 198, 452
OliI CACNNNNGTG 1 cut(s) 484
PagI TCATGA 1 cut(s) 696
PciI ACATGT 2 cut(s) 194, 448
PciSI GCTCTTC 1 cut(s) 573
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 205, 700
PkrI GCNGC 4 cut(s) 251, 254, 335, 389
Ppu21I YACGTR 1 cut(s) 781
PscI ACATGT 2 cut(s) 194, 448
Psp6I CCWGG 1 cut(s) 666
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 2 cut(s) 96, 161
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 254
RsaI GTAC 4 cut(s) 177, 453, 515, 565
RsaNI GTAC 4 cut(s) 176, 452, 514, 564
RseI CAYNNNNRTG 2 cut(s) 484, 728
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 111, 596
SatI GCNGC 4 cut(s) 250, 253, 334, 388
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 668
SduI GDGCHC 1 cut(s) 99
SetI ASST 7 cut(s) 111, 120, 413, 544, 585, 672, 783
SfaNI GCATC 1 cut(s) 143
SfcI CTRYAG 1 cut(s) 250
SfiI GGCCNNNNNGGCC 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 484, 728
SnaBI TACGTA 1 cut(s) 781
SpeI ACTAGT 1 cut(s) 622
Sse9I AATT 5 cut(s) 21, 259, 289, 588, 610
SsiI CCGC 5 cut(s) 126, 167, 294, 298, 387
SspI AATATT 1 cut(s) 760
SspMI CTAG 2 cut(s) 546, 623
StyD4I CCNGG 1 cut(s) 666
StyI CCWWGG 1 cut(s) 679
TaaI ACNGT 3 cut(s) 85, 214, 341
TaiI ACGT 1 cut(s) 783
TaqI TCGA 1 cut(s) 87
TasI AATT 5 cut(s) 21, 259, 289, 588, 610
TatI WGTACW 2 cut(s) 451, 563
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 2 cut(s) 205, 700
Tru1I TTAA 2 cut(s) 111, 596
Tru9I TTAA 2 cut(s) 111, 596
TscAI CASTG 2 cut(s) 238, 724
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 3 cut(s) 249, 252, 333
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 758
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 2 cut(s) 238, 724
XceI RCATGY 2 cut(s) 198, 452
XcmI CCANNNNNNNNNTGG 1 cut(s) 716
XspI CTAG 2 cut(s) 546, 623
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.