MD07G1060300.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000301
Physical Location & Seq
Forward (+)
5598028 .. 5598480
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1060300.v1.1.491

Sequence Viewer

Length: 453 bp
ATGAAAGCCTTCAAGGTTTTGCTTGTTCTTGCCATGCTACTGACTTTAACCATTAGTACTCTATCTGCAATCCCAGGCCTTGAAGAATCATTCTTCGAAGAGGAAAACAATGATCCAAATGATGAAACCAAAAGCCAATTAGAAAAAAGCACTTCTCTGAGGGGAACAAGCCGCTTCCTTGCTTCTCGGGCGGCAGTGATGACATGCGACAAACAACCTAGGGTTTGTCGGGTCTCGGGCAGCGGAGGCCCAGATTGCTGCAAGAAGAAATGTGTAAACACGAACACAGACAGAGTAAACTGTGGCAAGTGTGGGAACAAATGCAAGTACGCAGAGATATGCTGCAAGGGTAAGTGTGTGAATCCAAGGTCTGACAGGAAAAACTGTGGCGGCTGCAACAATAGGTGCAAGAAAGGCAGTTCATGTGCGTATGGCATGTGCAGTTATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

151

Amino Acids

16.28

Weight (kDa)

9.02

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 67 - 150 1e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 172, 191, 243, 390
AclWI GGATC 1 cut(s) 107
AfaI GTAC 2 cut(s) 58, 329
AgsI TTSAA 2 cut(s) 13, 83
AjnI CCWGG 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 2 cut(s) 186, 235
AoxI GGCC 2 cut(s) 76, 247
ApeKI GCWGC 4 cut(s) 240, 258, 342, 393
Asp700I GAANNNNTTC 1 cut(s) 8
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 248
AsuII TTCGAA 1 cut(s) 96
AvaI CYCGRG 2 cut(s) 186, 235
AvrII CCTAGG 1 cut(s) 218
BbvI GCAGC 4 cut(s) 245, 252, 329, 380
BciT130I CCWGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 1 cut(s) 219
BisI GCNGC 7 cut(s) 172, 192, 241, 259, 343, 391, 394
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 7 cut(s) 173, 193, 242, 260, 344, 392, 395
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 75
BmeT110I CYCGRG 2 cut(s) 186, 235
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 75
Bpu14I TTCGAA 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 3 cut(s) 73, 218, 365
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 73, 218, 365
BseMII CTCAG 1 cut(s) 149
BseXI GCAGC 4 cut(s) 245, 252, 329, 380
BshFI GGCC 2 cut(s) 78, 249
BsiHKCI CYCGRG 2 cut(s) 186, 235
BsmAI GTCTC 1 cut(s) 238
BsnI GGCC 2 cut(s) 78, 249
Bso31I GGTCTC 1 cut(s) 238
BsoBI CYCGRG 2 cut(s) 186, 235
Bsp119I TTCGAA 1 cut(s) 96
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 4 cut(s) 172, 191, 243, 390
BspANI GGCC 2 cut(s) 78, 249
BspCNI CTCAG 1 cut(s) 150
BspPI GGATC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 73, 218, 365
BssMI GATC 1 cut(s) 112
BssT1I CCWWGG 2 cut(s) 218, 365
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 302, 386
Bst6I CTCTTC 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 158
BstKTI GATC 1 cut(s) 115
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 112
BstMWI GCNNNNNNNGC 4 cut(s) 188, 246, 255, 414
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 2 cut(s) 207, 439
BstSCI CCNGG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 245, 252, 329, 380
BsuRI GGCC 2 cut(s) 78, 249
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 57, 328
CviAII CATG 4 cut(s) 34, 204, 423, 436
CviJI RGCY 6 cut(s) 8, 78, 135, 171, 249, 393
CviKI_1 RGCY 6 cut(s) 8, 78, 135, 171, 249, 393
CviQI GTAC 2 cut(s) 57, 328
DdeI CTNAG 1 cut(s) 158
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco130I CCWWGG 2 cut(s) 218, 365
Eco147I AGGCCT 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 238
Eco88I CYCGRG 2 cut(s) 186, 235
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 218, 365
ErhI CCWWGG 2 cut(s) 218, 365
FaeI CATG 4 cut(s) 37, 207, 426, 439
FaiI YATR 7 cut(s) 35, 205, 340, 424, 432, 437, 447
FatI CATG 4 cut(s) 33, 203, 422, 435
Fnu4HI GCNGC 7 cut(s) 172, 192, 241, 259, 343, 391, 394
Fsp4HI GCNGC 7 cut(s) 172, 192, 241, 259, 343, 391, 394
FspBI CTAG 1 cut(s) 219
GluI GCNGC 7 cut(s) 172, 192, 241, 259, 343, 391, 394
HaeIII GGCC 2 cut(s) 78, 249
Hin1II CATG 4 cut(s) 37, 207, 426, 439
HinfI GANTC 2 cut(s) 86, 361
Hpy166II GTNNAC 2 cut(s) 277, 298
Hpy188I TCNGA 2 cut(s) 159, 373
Hpy8I GTNNAC 2 cut(s) 277, 298
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 2 cut(s) 302, 386
HpyCH4V TGCA 7 cut(s) 68, 261, 324, 345, 396, 408, 441
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 246, 255, 414
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 4 cut(s) 37, 207, 426, 439
Kzo9I GATC 1 cut(s) 112
LpnPI CCDG 4 cut(s) 60, 87, 264, 361
Lsp1109I GCAGC 4 cut(s) 245, 252, 329, 380
MaeI CTAG 1 cut(s) 219
MalI GATC 1 cut(s) 114
MboI GATC 1 cut(s) 112
MboII GAAGA 4 cut(s) 85, 95, 110, 277
MluCI AATT 1 cut(s) 137
MnlI CCTC 3 cut(s) 94, 153, 239
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 4 cut(s) 188, 246, 255, 414
NdeII GATC 1 cut(s) 112
NlaIII CATG 4 cut(s) 37, 207, 426, 439
NspI RCATGY 2 cut(s) 207, 439
NspV TTCGAA 1 cut(s) 96
PceI AGGCCT 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 86, 361
PkrI GCNGC 7 cut(s) 173, 193, 242, 260, 344, 392, 395
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspPI GGNCC 1 cut(s) 248
RsaI GTAC 2 cut(s) 58, 329
RsaNI GTAC 2 cut(s) 57, 328
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 7 cut(s) 172, 192, 241, 259, 343, 391, 394
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 1 cut(s) 248
ScaI AGTACT 1 cut(s) 58
ScrFI CCNGG 1 cut(s) 75
SetI ASST 4 cut(s) 18, 220, 371, 407
SfuI TTCGAA 1 cut(s) 96
Sse9I AATT 1 cut(s) 137
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 4 cut(s) 172, 191, 243, 390
SspMI CTAG 1 cut(s) 219
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 2 cut(s) 218, 365
TaaI ACNGT 2 cut(s) 302, 386
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 137
TatI WGTACW 1 cut(s) 56
TauI GCSGC 3 cut(s) 174, 194, 393
TfiI GAWTC 2 cut(s) 86, 361
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 1 cut(s) 201
TseI GCWGC 4 cut(s) 240, 258, 342, 393
TspDTI ATGAA 3 cut(s) 17, 138, 411
TspRI CASTG 1 cut(s) 201
XceI RCATGY 2 cut(s) 207, 439
XmaJI CCTAGG 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 219
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.