MD05G1350300.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000301
Physical Location & Seq
Reverse (-)
46790064 .. 46790411
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1350300.v1.1.491

Sequence Viewer

Length: 348 bp
ATGATACAGATGATGCAACCAAAAGCATGCAGTCAGAAAAAACTACTTCTCTTATCCGGAAGAAGCCGCTTCCTTGCCTCTCGGGCCGCTGTGCCCACATGCGACAAATACCCTAGAGTTTGTCGGGCGACGGGCAGCGCAGGGCGAGATTGCTGCAAGAAGAAATGCGTGGATACGAACACAGACAGAGTAAACTGTGGCAAGTGCGGGAAGAAATGCAAGTACGGGGAGACATGCTGCAAAGGCAAGTGTGTGAACCCTAGGTCTGACAAGAAAAATTGTGGGAGCTGCAACAAAAAGTGCAAGAAAGGCAGTTCATGTGTCTATGGGATGTGCAGCTACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

116

Amino Acids

12.55

Weight (kDa)

9.59

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 33 - 115 1.4e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 3 cut(s) 67, 87, 207
AfaI GTAC 1 cut(s) 224
AluBI AGCT 2 cut(s) 288, 339
AluI AGCT 2 cut(s) 288, 339
Alw26I GTCTC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 5 cut(s) 135, 153, 237, 288, 336
AspA2I CCTAGG 1 cut(s) 260
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 84
AvaI CYCGRG 1 cut(s) 81
AvrII CCTAGG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 96
BbvI GCAGC 4 cut(s) 140, 147, 224, 275
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciVI GTATCC 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 2 cut(s) 114, 261
BfuI GTATCC 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 7 cut(s) 67, 87, 136, 154, 238, 289, 337
BlnI CCTAGG 1 cut(s) 260
BlsI GCNGC 7 cut(s) 68, 88, 137, 155, 239, 290, 338
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 84
BmsI GCATC 1 cut(s) 3
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 1 cut(s) 336
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 4 cut(s) 140, 147, 224, 275
BshFI GGCC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 56
BspACI CCGC 3 cut(s) 67, 87, 207
BspANI GGCC 1 cut(s) 86
BspEI TCCGGA 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 260
BssT1I CCWWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 28
BstF5I GGATG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 140
BstMAI GTCTC 1 cut(s) 224
BstMWI GCNNNNNNNGC 3 cut(s) 83, 243, 309
BstNSI RCATGY 3 cut(s) 30, 102, 237
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 140, 147, 224, 275
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 223
CviAII CATG 5 cut(s) 27, 99, 234, 318, 345
CviJI RGCY 4 cut(s) 66, 86, 288, 339
CviKI_1 RGCY 4 cut(s) 66, 86, 288, 339
CviQI GTAC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 5 cut(s) 30, 102, 237, 321, 348
FaiI YATR 6 cut(s) 28, 100, 235, 319, 327, 346
FatI CATG 5 cut(s) 26, 98, 233, 317, 344
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 7 cut(s) 67, 87, 136, 154, 238, 289, 337
FokI GGATG 1 cut(s) 343
Fsp4HI GCNGC 7 cut(s) 67, 87, 136, 154, 238, 289, 337
FspBI CTAG 2 cut(s) 114, 261
GlaI GCGC 1 cut(s) 139
GluI GCNGC 7 cut(s) 67, 87, 136, 154, 238, 289, 337
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 57
HhaI GCGC 1 cut(s) 140
Hin1II CATG 5 cut(s) 30, 102, 237, 321, 348
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HpaII CCGG 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 193, 256
Hpy188I TCNGA 2 cut(s) 36, 268
Hpy188III TCNNGA 1 cut(s) 57
Hpy8I GTNNAC 2 cut(s) 193, 256
Hpy99I CGWCG 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 8 cut(s) 16, 30, 156, 219, 240, 291, 303, 336
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 243, 309
Hsp92II CATG 5 cut(s) 30, 102, 237, 321, 348
HspAI GCGC 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 56
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 2 cut(s) 70, 126
Lsp1109I GCAGC 4 cut(s) 140, 147, 224, 275
LweI GCATC 1 cut(s) 3
MaeI CTAG 2 cut(s) 114, 261
MboII GAAGA 3 cut(s) 72, 172, 223
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 1 cut(s) 277
MnlI CCTC 1 cut(s) 88
MroI TCCGGA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 83, 243, 309
NlaIII CATG 5 cut(s) 30, 102, 237, 321, 348
NspI RCATGY 3 cut(s) 30, 102, 237
PaeI GCATGC 1 cut(s) 30
PkrI GCNGC 7 cut(s) 68, 88, 137, 155, 239, 290, 338
PspPI GGNCC 1 cut(s) 84
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SatI GCNGC 7 cut(s) 67, 87, 136, 154, 238, 289, 337
Sau96I GGNCC 1 cut(s) 84
SduI GDGCHC 1 cut(s) 96
SetI ASST 3 cut(s) 266, 290, 341
SfaNI GCATC 1 cut(s) 3
SphI GCATGC 1 cut(s) 30
Sse9I AATT 1 cut(s) 277
SsiI CCGC 3 cut(s) 67, 87, 207
SspMI CTAG 2 cut(s) 114, 261
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 197
TasI AATT 1 cut(s) 277
TauI GCSGC 2 cut(s) 69, 89
TseI GCWGC 5 cut(s) 135, 153, 237, 288, 336
TspDTI ATGAA 1 cut(s) 306
XceI RCATGY 3 cut(s) 30, 102, 237
XmaJI CCTAGG 1 cut(s) 260
XspI CTAG 2 cut(s) 114, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.