Rroxscaffold_5G00372420

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000301
Physical Location & Seq
Reverse (-)
53851448 .. 53851891
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372420.1

Sequence Viewer

Length: 444 bp
ATGGCTTTAGCCGTTACTCTCTCTGCAACACTAGTCGAAGAAGAACTATTTTCCAACAAGGAAACCAAGGCTGCAAATGATCACGAAACCTTTGATGATGATCTTCCATTGGATGCCAAAAGCCAAGGGAAAACCTCTTTGAGGGGAACAAGCAGCTTCTTCCTTGGTTCTAGGGAAGTGACGACATCATCATGCGACAAAAACCCTAAGGTTTGTAGAGCCGCCGCAAACAGCCCAAAATCACGTTGCTGCCACAACAAGTGTGTGGACGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTGAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGTGCACACAATGTCCGACGAGAAACACTGCGGGAGCTGCGACAACCATTGCAAGAGAGGTGGTTCTTGTGCGTTTGGGATGTGCAGCTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

147

Amino Acids

15.83

Weight (kDa)

7.89

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 63 - 147 1e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 222, 225, 294, 378
AfiI CCNNNNNNNGG 1 cut(s) 141
AhlI ACTAGT 1 cut(s) 31
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 3 cut(s) 156, 384, 435
AluI AGCT 3 cut(s) 156, 384, 435
Alw21I GWGCWC 1 cut(s) 354
Alw44I GTGCAC 1 cut(s) 350
ApaLI GTGCAC 1 cut(s) 350
ApeKI GCWGC 6 cut(s) 71, 153, 249, 333, 384, 432
AsuHPI GGTGA 1 cut(s) 319
AxyI CCTNAGG 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 58, 165, 236, 320, 371
BclI TGATCA 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 2 cut(s) 32, 171
BisI GCNGC 8 cut(s) 72, 154, 222, 225, 250, 334, 385, 433
BlsI GCNGC 8 cut(s) 73, 155, 223, 226, 251, 335, 386, 434
BmgBI CACGTC 1 cut(s) 271
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 66, 124, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 99
BseDI CCNNGG 3 cut(s) 66, 124, 163
BseGI GGATG 2 cut(s) 118, 432
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 394
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 5 cut(s) 58, 165, 236, 320, 371
BsiHKAI GWGCWC 1 cut(s) 354
BslI CCNNNNNNNGG 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 2 cut(s) 79, 100
BspACI CCGC 4 cut(s) 222, 225, 294, 378
BsrDI GCAATG 1 cut(s) 394
BssECI CCNNGG 3 cut(s) 66, 124, 163
BssMI GATC 2 cut(s) 79, 100
BssT1I CCWWGG 3 cut(s) 66, 124, 163
Bst4CI ACNGT 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 317, 321
BstDEI CTNAG 2 cut(s) 207, 441
BstENI CCTNNNNNAGG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 118, 432
BstKTI GATC 2 cut(s) 82, 103
BstMBI GATC 2 cut(s) 79, 100
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 354
BstV1I GCAGC 5 cut(s) 58, 165, 236, 320, 371
BstV2I GAAGAC 1 cut(s) 281
Bsu36I CCTNAGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 2 cut(s) 118, 432
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 373
Cac8I GCNNGC 2 cut(s) 317, 321
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 2 cut(s) 192, 344
CviJI RGCY 9 cut(s) 5, 11, 71, 123, 156, 221, 234, 384, 435
CviKI_1 RGCY 9 cut(s) 5, 11, 71, 123, 156, 221, 234, 384, 435
DdeI CTNAG 2 cut(s) 207, 441
DpnI GATC 2 cut(s) 81, 102
DpnII GATC 2 cut(s) 79, 100
Eco130I CCWWGG 3 cut(s) 66, 124, 163
Eco81I CCTNAGG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 66, 124, 163
ErhI CCWWGG 3 cut(s) 66, 124, 163
FaeI CATG 2 cut(s) 195, 347
FaiI YATR 4 cut(s) 193, 331, 345, 438
FatI CATG 2 cut(s) 191, 343
FauI CCCGC 2 cut(s) 287, 371
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 8 cut(s) 72, 154, 222, 225, 250, 334, 385, 433
FokI GGATG 2 cut(s) 125, 439
Fsp4HI GCNGC 8 cut(s) 72, 154, 222, 225, 250, 334, 385, 433
FspBI CTAG 2 cut(s) 32, 171
GluI GCNGC 8 cut(s) 72, 154, 222, 225, 250, 334, 385, 433
Hin1II CATG 2 cut(s) 195, 347
HinfI GANTC 1 cut(s) 285
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 268, 352
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 2 cut(s) 268, 352
Hpy99I CGWCG 1 cut(s) 368
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 244, 270
HpyCH4V TGCA 7 cut(s) 26, 74, 315, 336, 352, 399, 432
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpyF3I CTNAG 2 cut(s) 207, 441
HpySE526I ACGT 2 cut(s) 244, 270
Hsp92II CATG 2 cut(s) 195, 347
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 79, 100
LmnI GCTCC 1 cut(s) 381
Lsp1109I GCAGC 5 cut(s) 58, 165, 236, 320, 371
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 32, 171
MaeII ACGT 2 cut(s) 244, 270
MaeIII GTNAC 2 cut(s) 13, 178
MalI GATC 2 cut(s) 81, 102
MboI GATC 2 cut(s) 79, 100
MboII GAAGA 5 cut(s) 50, 53, 95, 151, 286
MhlI GDGCHC 1 cut(s) 354
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 78, 387
MnlI CCTC 3 cut(s) 135, 145, 398
MslI CAYNNNNRTG 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 384
NdeII GATC 2 cut(s) 79, 100
NlaIII CATG 2 cut(s) 195, 347
NmuCI GTSAC 1 cut(s) 178
PkrI GCNGC 8 cut(s) 73, 155, 223, 226, 251, 335, 386, 434
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 190
SatI GCNGC 8 cut(s) 72, 154, 222, 225, 250, 334, 385, 433
Sau3AI GATC 2 cut(s) 79, 100
SchI GAGTC 1 cut(s) 279
SduI GDGCHC 1 cut(s) 354
SetI ASST 9 cut(s) 92, 137, 158, 213, 247, 273, 386, 409, 437
SfaNI GCATC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 190
SpeI ACTAGT 1 cut(s) 31
SsiI CCGC 4 cut(s) 222, 225, 294, 378
SspMI CTAG 2 cut(s) 32, 171
StyI CCWWGG 3 cut(s) 66, 124, 163
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 247, 273
TaqI TCGA 1 cut(s) 36
TauI GCSGC 2 cut(s) 224, 227
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 6 cut(s) 71, 153, 249, 333, 384, 432
Tsp45I GTSAC 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 1 cut(s) 380
VneI GTGCAC 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 139
XspI CTAG 2 cut(s) 32, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.