RchiOBHm_Chr5g0030071

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000301
Physical Location & Seq
Reverse (-)
23857739 .. 23858188
450 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30943

Sequence Viewer

Length: 450 bp
ATGAAGTTGATGAACGTCTCCTTTATAGTACTCCTAGCAACAATGGCCATTGTTTCAGCTGAATATCTAGACGATTACAACAATGAAGAAATGCAAATTCAAAATACCGAATCAACAGTGTCTGAAATGCAAGAAGCAACAACTTCTTTGAGAGGGGTAAGCCGCTTCCTGACCCAGCAGAACCCGTTGGCGAGCGTGACTTGCGACAAGTTACCTAGGGTTTGTCGTCTGAAGAAGAGCCCGGGGTCTGACTGCTGCAAGAAGAAGTGTGTGAATTTGAAGACGGATAGATTGAATTGCGGGATGTGCGGGTACAAGTGCAAGTACACTGAGATTTGCTGCAGGGGAAAATGCGTGAATGCATCGTTTGACGAAAAGCATTGCGGTGGATGCAACCAGAAGTGCAAGAAAGGGGAGTTTTGTGTTTTGGGGATGTGCAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

149

Amino Acids

16.64

Weight (kDa)

8.57

Isoelectric Point (pI)

19.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 66 - 149 5.6e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 163, 300, 309, 384
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 2 cut(s) 96, 274
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 3 cut(s) 30, 314, 326
AgsI TTSAA 3 cut(s) 101, 280, 295
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 22
AlwNI CAGNNNCTG 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 255, 339
ApoI RAATTY 2 cut(s) 96, 274
AspA2I CCTAGG 1 cut(s) 215
AsuC2I CCSGG 2 cut(s) 242, 243
AvaI CYCGRG 1 cut(s) 241
AvrII CCTAGG 1 cut(s) 215
BalI TGGCCA 1 cut(s) 47
BanII GRGCYC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 242, 326
BcnI CCSGG 2 cut(s) 242, 243
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 3 cut(s) 35, 68, 216
BfmI CTRYAG 1 cut(s) 340
BisI GCNGC 3 cut(s) 163, 256, 340
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 3 cut(s) 164, 257, 341
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 2 cut(s) 242, 243
BmeT110I CYCGRG 1 cut(s) 241
BmrFI CCNGG 2 cut(s) 242, 243
BmsI GCATC 2 cut(s) 371, 380
BpiI GAAGAC 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 242, 243
BsaJI CCNNGG 3 cut(s) 215, 241, 242
Bse3DI GCAATG 1 cut(s) 379
BseDI CCNNGG 3 cut(s) 215, 241, 242
BseGI GGATG 3 cut(s) 309, 395, 438
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 321
BseXI GCAGC 2 cut(s) 242, 326
BseYI CCCAGC 1 cut(s) 174
BshFI GGCC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 1 cut(s) 242
BsmAI GTCTC 1 cut(s) 22
BsmBI CGTCTC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 242
BspACI CCGC 4 cut(s) 163, 300, 309, 384
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 322
BspMAI CTGCAG 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 379
BssECI CCNNGG 3 cut(s) 215, 241, 242
BssT1I CCWWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 309, 395, 438
BstMAI GTCTC 1 cut(s) 22
BstMWI GCNNNNNNNGC 4 cut(s) 44, 201, 306, 390
BstSCI CCNGG 2 cut(s) 240, 241
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 242, 326
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 47
BtsCI GGATG 3 cut(s) 309, 395, 438
BtsIMutI CAGTG 2 cut(s) 123, 327
Cac8I GCNNGC 1 cut(s) 193
CaiI CAGNNNCTG 1 cut(s) 122
Cfr9I CCCGGG 1 cut(s) 241
Csp6I GTAC 3 cut(s) 29, 313, 325
CviAII CATG 1 cut(s) 447
CviJI RGCY 4 cut(s) 47, 59, 162, 240
CviKI_1 RGCY 4 cut(s) 47, 59, 162, 240
CviQI GTAC 3 cut(s) 29, 313, 325
DdeI CTNAG 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 215
EcoT22I ATGCAT 2 cut(s) 364, 448
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 215
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 1 cut(s) 450
FaiI YATR 3 cut(s) 26, 444, 448
FatI CATG 1 cut(s) 446
FauI CCCGC 2 cut(s) 293, 302
Fnu4HI GCNGC 3 cut(s) 163, 256, 340
FokI GGATG 3 cut(s) 316, 402, 445
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 3 cut(s) 163, 256, 340
FspBI CTAG 3 cut(s) 35, 68, 216
GluI GCNGC 3 cut(s) 163, 256, 340
GsaI CCCAGC 1 cut(s) 178
HaeIII GGCC 1 cut(s) 47
HapII CCGG 1 cut(s) 242
Hin1II CATG 1 cut(s) 450
HinfI GANTC 1 cut(s) 110
HpaII CCGG 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 3 cut(s) 124, 231, 250
Hpy188III TCNNGA 2 cut(s) 68, 169
Hpy8I GTNNAC 1 cut(s) 327
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 201, 306, 390
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 1 cut(s) 450
LguI GCTCTTC 1 cut(s) 230
LpnPI CCDG 5 cut(s) 182, 188, 255, 328, 410
Lsp1109I GCAGC 2 cut(s) 242, 326
LweI GCATC 2 cut(s) 371, 380
MaeI CTAG 3 cut(s) 35, 68, 216
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 196, 210
MboII GAAGA 5 cut(s) 98, 244, 247, 274, 292
MhlI GDGCHC 1 cut(s) 242
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 4 cut(s) 96, 274, 295, 439
MluNI TGGCCA 1 cut(s) 47
MnlI CCTC 1 cut(s) 146
Mox20I TGGCCA 1 cut(s) 47
Mph1103I ATGCAT 2 cut(s) 364, 448
MscI TGGCCA 1 cut(s) 47
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 2 cut(s) 242, 243
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 4 cut(s) 44, 201, 306, 390
NciI CCSGG 2 cut(s) 242, 243
NlaIII CATG 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 2 cut(s) 364, 448
PciSI GCTCTTC 1 cut(s) 230
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 164, 257, 341
PspFI CCCAGC 1 cut(s) 174
PstI CTGCAG 1 cut(s) 344
PstNI CAGNNNCTG 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 3 cut(s) 30, 314, 326
RsaNI GTAC 3 cut(s) 29, 313, 325
RseI CAYNNNNRTG 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 230
SatI GCNGC 3 cut(s) 163, 256, 340
ScaI AGTACT 1 cut(s) 30
ScrFI CCNGG 2 cut(s) 242, 243
SduI GDGCHC 1 cut(s) 242
SetI ASST 3 cut(s) 18, 61, 217
SfaNI GCATC 2 cut(s) 371, 380
SfcI CTRYAG 1 cut(s) 340
SmaI CCCGGG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 4 cut(s) 96, 274, 295, 439
SsiI CCGC 4 cut(s) 163, 300, 309, 384
SspMI CTAG 3 cut(s) 35, 68, 216
StyD4I CCNGG 2 cut(s) 240, 241
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 1 cut(s) 18
TasI AATT 4 cut(s) 96, 274, 295, 439
TatI WGTACW 2 cut(s) 28, 324
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 1 cut(s) 110
TscAI CASTG 2 cut(s) 123, 334
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 255, 339
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 17, 26, 99
TspGWI ACGGA 1 cut(s) 299
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 2 cut(s) 123, 334
XapI RAATTY 2 cut(s) 96, 274
XbaI TCTAGA 1 cut(s) 67
XmaI CCCGGG 1 cut(s) 241
XmaJI CCTAGG 1 cut(s) 215
XspI CTAG 3 cut(s) 35, 68, 216
ZrmI AGTACT 1 cut(s) 30
Zsp2I ATGCAT 2 cut(s) 364, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.