Prupe.4G016900_v2.0.a1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000301
Physical Location & Seq
Forward (+)
801833 .. 802291
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G016900.1

Sequence Viewer

Length: 459 bp
ATGAAGTTGATGAACATCTTCGTTACACTCCTAGCAATGATAGCCATTGTTGCAGCTGCAAATTTGGACGAAGATTACAACGAGGACGTGGAAATGCAGAGTACTGAAGCAACATTGCCTGAAACACCAGACGAAGGAGAAGCAACTACTTCTTTAAGAGGAGTGAGCCGTTTTCTTTATCAGAAGAATGTGCAACAGGCCGATTATACTTGCAACAAGTTTCCTAGGGTTTGTCGTTTGAGGAACAGTCCAGGGCCAGACTGCTGCAAGAAGAAATGTGTTAACGTGAAGACGGATCGATACAACTGTGGATTTTGCGGCTACAGATGCAAGTACACTGAGATTTGCTGCAGGGGAAAGTGCGTCAATGCATCTTTTGACAAGAGGCATTGTGGTGGGTGCAATCAGAAGTGCAAGAAGGGAGAGTACTGTGTTTTTGGGATGTGCAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

153

Amino Acids

17.16

Weight (kDa)

8.44

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 318
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 3 cut(s) 103, 335, 428
AfiI CCNNNNNNNGG 1 cut(s) 134
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
AlwI GGATC 1 cut(s) 303
AoxI GGCC 2 cut(s) 198, 254
ApeKI GCWGC 4 cut(s) 53, 56, 264, 348
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 17
AspA2I CCTAGG 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 254
AvrII CCTAGG 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BarI GAAGNNNNNNTAC 2 cut(s) 410, 442
BbsI GAAGAC 1 cut(s) 296
BbvI GCAGC 4 cut(s) 43, 65, 251, 335
BceAI ACGGC 1 cut(s) 153
BciT130I CCWGG 1 cut(s) 252
BfaI CTAG 2 cut(s) 32, 225
BfmI CTRYAG 2 cut(s) 322, 349
BisI GCNGC 5 cut(s) 54, 57, 265, 319, 349
BlnI CCTAGG 1 cut(s) 224
BlsI GCNGC 5 cut(s) 55, 58, 266, 320, 350
BmcAI AGTACT 2 cut(s) 103, 428
Bme1390I CCNGG 1 cut(s) 252
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 317, 380
BpiI GAAGAC 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 224, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse3DI GCAATG 2 cut(s) 42, 113
Bse8I GATNNNNATC 1 cut(s) 14
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 224, 251
BseGI GGATG 1 cut(s) 447
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 2 cut(s) 42, 113
BseMII CTCAG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 4 cut(s) 43, 65, 251, 335
BshFI GGCC 2 cut(s) 200, 256
BshVI ATCGAT 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 134
BsnI GGCC 2 cut(s) 200, 256
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 1 cut(s) 318
BspANI GGCC 2 cut(s) 200, 256
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 298
BspMAI CTGCAG 1 cut(s) 353
BspPI GGATC 1 cut(s) 303
BsrDI GCAATG 2 cut(s) 42, 113
BssECI CCNNGG 2 cut(s) 224, 251
BssMI GATC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 248, 308, 431
BstDEI CTNAG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 447
BstKTI GATC 1 cut(s) 298
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 3 cut(s) 41, 50, 327
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 2 cut(s) 322, 349
BstV1I GCAGC 4 cut(s) 43, 65, 251, 335
BstV2I GAAGAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 298
BsuRI GGCC 2 cut(s) 200, 256
BsuTUI ATCGAT 1 cut(s) 298
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 447
BtsIMutI CAGTG 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 254
ClaI ATCGAT 1 cut(s) 298
CseI GACGC 1 cut(s) 352
Csp6I GTAC 3 cut(s) 102, 334, 427
CviAII CATG 1 cut(s) 456
CviJI RGCY 6 cut(s) 44, 56, 168, 200, 256, 321
CviKI_1 RGCY 6 cut(s) 44, 56, 168, 200, 256, 321
CviQI GTAC 3 cut(s) 102, 334, 427
DdeI CTNAG 1 cut(s) 339
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 224
EcoT22I ATGCAT 2 cut(s) 373, 457
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 1 cut(s) 459
FaiI YATR 3 cut(s) 207, 453, 457
FatI CATG 1 cut(s) 455
Fnu4HI GCNGC 5 cut(s) 54, 57, 265, 319, 349
FokI GGATG 1 cut(s) 454
Fsp4HI GCNGC 5 cut(s) 54, 57, 265, 319, 349
FspBI CTAG 2 cut(s) 32, 225
GluI GCNGC 5 cut(s) 54, 57, 265, 319, 349
HaeIII GGCC 2 cut(s) 200, 256
HgaI GACGC 1 cut(s) 352
Hin1II CATG 1 cut(s) 459
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HpaI GTTAAC 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 283, 336
Hpy188I TCNGA 2 cut(s) 183, 408
Hpy8I GTNNAC 2 cut(s) 283, 336
HpyAV CCTTC 2 cut(s) 128, 412
HpyCH4III ACNGT 3 cut(s) 248, 308, 431
HpyCH4IV ACGT 2 cut(s) 87, 285
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 50, 327
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 2 cut(s) 87, 285
Hsp92II CATG 1 cut(s) 459
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 1 cut(s) 295
LpnPI CCDG 7 cut(s) 132, 141, 182, 237, 264, 270, 337
Lsp1109I GCAGC 4 cut(s) 43, 65, 251, 335
LweI GCATC 2 cut(s) 317, 380
MaeI CTAG 2 cut(s) 32, 225
MaeII ACGT 2 cut(s) 87, 285
MaeIII GTNAC 1 cut(s) 22
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 5 cut(s) 10, 83, 196, 283, 301
MluCI AATT 2 cut(s) 61, 448
MnlI CCTC 4 cut(s) 76, 152, 234, 378
Mph1103I ATGCAT 2 cut(s) 373, 457
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 155, 282
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 41, 50, 327
NdeII GATC 1 cut(s) 295
NlaIII CATG 1 cut(s) 459
NsiI ATGCAT 2 cut(s) 373, 457
PdmI GAANNNNTTC 1 cut(s) 17
PkrI GCNGC 5 cut(s) 55, 58, 266, 320, 350
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspPI GGNCC 1 cut(s) 254
PstI CTGCAG 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 3 cut(s) 103, 335, 428
RsaNI GTAC 3 cut(s) 102, 334, 427
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 2 cut(s) 155, 282
SatI GCNGC 5 cut(s) 54, 57, 265, 319, 349
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 1 cut(s) 254
ScaI AGTACT 2 cut(s) 103, 428
ScrFI CCNGG 1 cut(s) 252
SetI ASST 3 cut(s) 58, 90, 288
SfaNI GCATC 2 cut(s) 317, 380
SfcI CTRYAG 2 cut(s) 322, 349
SmiMI CAYNNNNRTG 1 cut(s) 393
Sse9I AATT 2 cut(s) 61, 448
SsiI CCGC 1 cut(s) 318
SspMI CTAG 2 cut(s) 32, 225
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 248, 308, 431
TaiI ACGT 2 cut(s) 90, 288
TaqI TCGA 1 cut(s) 298
TasI AATT 2 cut(s) 61, 448
TatI WGTACW 3 cut(s) 101, 333, 426
TauI GCSGC 1 cut(s) 321
Tru1I TTAA 2 cut(s) 155, 282
Tru9I TTAA 2 cut(s) 155, 282
TscAI CASTG 1 cut(s) 343
TseI GCWGC 4 cut(s) 53, 56, 264, 348
TspDTI ATGAA 2 cut(s) 17, 26
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 343
XapI RAATTY 1 cut(s) 61
XmaJI CCTAGG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 2 cut(s) 32, 225
ZrmI AGTACT 2 cut(s) 103, 428
Zsp2I ATGCAT 2 cut(s) 373, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.