Rw5G001800

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000301
Physical Location & Seq
Forward (+)
1558405 .. 1558851
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001800.1

Sequence Viewer

Length: 447 bp
ATGGAAATGATAGTATCCAAAATTGTGTTCACCCTAATCTTCGTGTTGTTGGGTATAGCCATTGCCCATTCAGCAACACCAACTGTCGAAGAGGAGCCCCAACTCAACCTTAACGAGCTTTCATCCGCAATGGCATCTCTGCGAGGGATTGGTGGCCGGTTCCTTGCGGAGCAAGTACGAGCCACACCGACCACATGTGACAAGAATCCGACATTCTGTAAGAGCAAGGGCAGTGCAGGGCCTAACTGCTGCAGCAAAAATTGCGTCAATGTAACGACGGATACAAACAATTGCGGGCGGTGTGGAGTGAAGTGTAAGTACTCGGAACTCTGCTGCAACGGTGTTTGTGTGAATCCGTCTGTCAATGGCAAACATTGTGGCAAGTGCGGCAACAAGTGTGAAAAAGGAAGCTCATGCTTGTATGGCCTGTGCAGCTATGCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

148

Amino Acids

15.63

Weight (kDa)

8.47

Isoelectric Point (pI)

19.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 64 - 147 4.8e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 126, 167, 294, 298, 387
AcoI YGGCCR 1 cut(s) 154
AfaI GTAC 2 cut(s) 177, 320
AflIII ACRYGT 1 cut(s) 194
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 3 cut(s) 118, 411, 435
AluI AGCT 3 cut(s) 118, 411, 435
AoxI GGCC 3 cut(s) 154, 239, 424
ApeKI GCWGC 4 cut(s) 249, 252, 333, 432
ArsI GACNNNNNNTTYG 2 cut(s) 249, 281
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 22
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
BbvI GCAGC 3 cut(s) 236, 264, 320
BciVI GTATCC 2 cut(s) 25, 274
BfmI CTRYAG 1 cut(s) 250
BfuI GTATCC 2 cut(s) 25, 274
BisI GCNGC 5 cut(s) 250, 253, 334, 388, 433
BlsI GCNGC 5 cut(s) 251, 254, 335, 389, 434
BmcAI AGTACT 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 96, 161
BmsI GCATC 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 156
Bse3DI GCAATG 2 cut(s) 60, 135
BseGI GGATG 1 cut(s) 122
BseMI GCAATG 2 cut(s) 60, 135
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 3 cut(s) 236, 264, 320
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 3 cut(s) 156, 241, 426
BsiSI CCGG 1 cut(s) 157
BsnI GGCC 3 cut(s) 156, 241, 426
Bsp1286I GDGCHC 1 cut(s) 99
BspACI CCGC 5 cut(s) 126, 167, 294, 298, 387
BspANI GGCC 3 cut(s) 156, 241, 426
BspLI GGNNCC 2 cut(s) 96, 161
BspMAI CTGCAG 1 cut(s) 254
BsrDI GCAATG 2 cut(s) 60, 135
BsrFI RCCGGY 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 85, 341
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 296
BstF5I GGATG 1 cut(s) 122
BstMWI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 423, 432
BstNSI RCATGY 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 236, 264, 320
BsuI GTATCC 2 cut(s) 25, 274
BsuRI GGCC 3 cut(s) 156, 241, 426
BtsCI GGATG 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 176, 319
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 2 cut(s) 195, 414
CviJI RGCY 9 cut(s) 59, 97, 118, 156, 182, 241, 411, 426, 435
CviKI_1 RGCY 9 cut(s) 59, 97, 118, 156, 182, 241, 411, 426, 435
CviQI GTAC 2 cut(s) 176, 319
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 2 cut(s) 198, 417
FaiI YATR 5 cut(s) 56, 196, 415, 423, 438
FatI CATG 2 cut(s) 194, 413
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 5 cut(s) 250, 253, 334, 388, 433
FokI GGATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 5 cut(s) 250, 253, 334, 388, 433
GluI GCNGC 5 cut(s) 250, 253, 334, 388, 433
HaeIII GGCC 3 cut(s) 156, 241, 426
HapII CCGG 1 cut(s) 157
HgaI GACGC 1 cut(s) 253
Hin1II CATG 2 cut(s) 198, 417
HinfI GANTC 2 cut(s) 205, 352
HpaII CCGG 1 cut(s) 157
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 210, 325
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 280
HpyCH4III ACNGT 2 cut(s) 85, 341
HpyCH4V TGCA 4 cut(s) 236, 252, 336, 432
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 423, 432
Hsp92II CATG 2 cut(s) 198, 417
LmnI GCTCC 2 cut(s) 94, 169
LpnPI CCDG 3 cut(s) 170, 222, 440
Lsp1109I GCAGC 3 cut(s) 236, 264, 320
LweI GCATC 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 197, 271
MboII GAAGA 2 cut(s) 31, 101
MfeI CAATTG 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 4 cut(s) 21, 259, 289, 442
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 2 cut(s) 85, 137
MseI TTAA 1 cut(s) 111
MspI CCGG 1 cut(s) 157
MunI CAATTG 1 cut(s) 289
MwoI GCNNNNNNNGC 5 cut(s) 71, 261, 387, 423, 432
NlaIII CATG 2 cut(s) 198, 417
NlaIV GGNNCC 2 cut(s) 96, 161
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 1 cut(s) 198
PciI ACATGT 1 cut(s) 194
PfeI GAWTC 2 cut(s) 205, 352
PkrI GCNGC 5 cut(s) 251, 254, 335, 389, 434
PscI ACATGT 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 96, 161
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 254
RsaI GTAC 2 cut(s) 177, 320
RsaNI GTAC 2 cut(s) 176, 319
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 5 cut(s) 250, 253, 334, 388, 433
Sau96I GGNCC 1 cut(s) 239
ScaI AGTACT 1 cut(s) 320
SduI GDGCHC 1 cut(s) 99
SetI ASST 4 cut(s) 111, 120, 413, 437
SfaNI GCATC 1 cut(s) 143
SfcI CTRYAG 1 cut(s) 250
Sse9I AATT 4 cut(s) 21, 259, 289, 442
SsiI CCGC 5 cut(s) 126, 167, 294, 298, 387
TaaI ACNGT 2 cut(s) 85, 341
TaqI TCGA 1 cut(s) 87
TasI AATT 4 cut(s) 21, 259, 289, 442
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 2 cut(s) 205, 352
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 4 cut(s) 249, 252, 333, 432
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 111
TspGWI ACGGA 2 cut(s) 293, 345
TspRI CASTG 1 cut(s) 238
XceI RCATGY 1 cut(s) 198
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.