Rh5AG020000

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000301
Physical Location & Seq
Forward (+)
1423208 .. 1423651
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG020000.1

Sequence Viewer

Length: 444 bp
ATGAACTGTCATAAGGTTTTCCTTCTGCTAGCCATGCTGATGATGGCTTCAGCCGGTACGCTTTCTGCGACACTAGACGAAGAAGAATCATCAATCTTCAACGAGGATAACAACGTCCAAAACCAAGAAAAAACTTCTCTCAGGGGAACAAGCAGCGTCTTCTTTGCTTCTCGGTCAGTGACAGCGTCAACATGTGACAAAAACCCTAAGGTTTGTGGCGCGGCTGCCGCGGACGGCTCGGAGTGCTGCAGTAAGAATTGCGTGGATTTGAAGACGGACAGACTCAATTGCGGGAAATGTGGGGTGAAATGCAAGCAGAGTACAGAGATATGCTGCAATGGTCATATTGTGACTCCAATGTCTGACAAGAAAAACTGTGGGAGATGCAACAATGCTTGCAAGGGACGCAACTCATGCGCGTTTGGGATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

147

Amino Acids

15.67

Weight (kDa)

8.13

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 63 - 147 1.4e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 358
AccII CGCG 3 cut(s) 221, 230, 419
AciI CCGC 4 cut(s) 221, 228, 230, 291
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 2 cut(s) 58, 322
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 100, 271
AluBI AGCT 1 cut(s) 435
AluI AGCT 1 cut(s) 435
ApeKI GCWGC 5 cut(s) 153, 224, 246, 333, 432
AspLEI GCGC 2 cut(s) 221, 419
AsuHPI GGTGA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 28
AxyI CCTNAGG 1 cut(s) 207
BbsI GAAGAC 2 cut(s) 151, 278
BbvI GCAGC 4 cut(s) 165, 211, 233, 320
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 250
BfaI CTAG 2 cut(s) 29, 74
BfmI CTRYAG 1 cut(s) 247
BisI GCNGC 7 cut(s) 154, 222, 225, 228, 247, 334, 433
BlsI GCNGC 7 cut(s) 155, 223, 226, 229, 248, 335, 434
BmsI GCATC 1 cut(s) 374
BmtI GCTAGC 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 151, 278
BsaJI CCNNGG 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 53
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 343
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 154
BseXI GCAGC 4 cut(s) 165, 211, 233, 320
Bsh1236I CGCG 3 cut(s) 221, 230, 419
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 417
BspACI CCGC 4 cut(s) 221, 228, 230, 291
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 3 cut(s) 221, 230, 419
BspMAI CTGCAG 1 cut(s) 251
BspOI GCTAGC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 8, 377
BstAPI GCANNNNNTGC 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 30, 314, 397
BstDEI CTNAG 2 cut(s) 140, 207
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 432
BstFNI CGCG 3 cut(s) 221, 230, 419
BstHHI GCGC 2 cut(s) 221, 419
BstMWI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 405, 414
BstNSI RCATGY 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 3 cut(s) 221, 230, 419
BstV1I GCAGC 4 cut(s) 165, 211, 233, 320
BstV2I GAAGAC 2 cut(s) 151, 278
Bsu36I CCTNAGG 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 30, 314, 397
CfoI GCGC 2 cut(s) 221, 419
Cfr10I RCCGGY 1 cut(s) 53
Cfr42I CCGCGG 1 cut(s) 231
CseI GACGC 3 cut(s) 145, 174, 414
Csp6I GTAC 2 cut(s) 57, 321
CviAII CATG 3 cut(s) 34, 192, 414
CviJI RGCY 6 cut(s) 32, 47, 53, 224, 237, 435
CviKI_1 RGCY 6 cut(s) 32, 47, 53, 224, 237, 435
CviQI GTAC 2 cut(s) 57, 321
DdeI CTNAG 2 cut(s) 140, 207
DrdI GACNNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 442
FaeI CATG 3 cut(s) 37, 195, 417
FaiI YATR 8 cut(s) 12, 35, 193, 331, 345, 415, 438, 442
FaqI GGGAC 1 cut(s) 417
FatI CATG 3 cut(s) 33, 191, 413
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 7 cut(s) 154, 222, 225, 228, 247, 334, 433
FokI GGATG 1 cut(s) 439
Fsp4HI GCNGC 7 cut(s) 154, 222, 225, 228, 247, 334, 433
FspBI CTAG 2 cut(s) 29, 74
GlaI GCGC 2 cut(s) 220, 418
GluI GCNGC 7 cut(s) 154, 222, 225, 228, 247, 334, 433
HapII CCGG 1 cut(s) 54
HgaI GACGC 3 cut(s) 145, 174, 414
HhaI GCGC 2 cut(s) 221, 419
Hin1II CATG 3 cut(s) 37, 195, 417
Hin6I GCGC 2 cut(s) 219, 417
HinP1I GCGC 2 cut(s) 219, 417
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 3 cut(s) 86, 282, 352
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 2 cut(s) 241, 364
Hpy8I GTNNAC 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 2 cut(s) 8, 377
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 7 cut(s) 249, 312, 336, 387, 399, 432, 440
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 405, 414
HpyF3I CTNAG 2 cut(s) 140, 207
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 3 cut(s) 37, 195, 417
HspAI GCGC 2 cut(s) 219, 417
KspI CCGCGG 1 cut(s) 231
LpnPI CCDG 2 cut(s) 67, 127
Lsp1109I GCAGC 4 cut(s) 165, 211, 233, 320
LweI GCATC 1 cut(s) 374
MaeI CTAG 2 cut(s) 29, 74
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 178, 194, 349
MboII GAAGA 5 cut(s) 88, 92, 95, 151, 283
MfeI CAATTG 1 cut(s) 286
MluCI AATT 2 cut(s) 256, 286
MlyI GAGTC 2 cut(s) 276, 346
MnlI CCTC 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 442
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 54
MunI CAATTG 1 cut(s) 286
MvnI CGCG 3 cut(s) 221, 230, 419
MwoI GCNNNNNNNGC 5 cut(s) 34, 227, 243, 405, 414
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 3 cut(s) 37, 195, 417
NmuCI GTSAC 3 cut(s) 178, 194, 349
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 195
PciI ACATGT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 1 cut(s) 86
PflFI GACNNNGTC 1 cut(s) 184
PkrI GCNGC 7 cut(s) 155, 223, 226, 229, 248, 335, 434
PleI GAGTC 2 cut(s) 276, 346
PpsI GAGTC 2 cut(s) 276, 346
PscI ACATGT 1 cut(s) 191
PstI CTGCAG 1 cut(s) 251
PsyI GACNNNGTC 1 cut(s) 184
RsaI GTAC 2 cut(s) 58, 322
RsaNI GTAC 2 cut(s) 57, 321
RseI CAYNNNNRTG 1 cut(s) 38
SacII CCGCGG 1 cut(s) 231
SatI GCNGC 7 cut(s) 154, 222, 225, 228, 247, 334, 433
SchI GAGTC 2 cut(s) 276, 346
SetI ASST 4 cut(s) 18, 117, 213, 437
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 38
Sse9I AATT 2 cut(s) 256, 286
SsiI CCGC 4 cut(s) 221, 228, 230, 291
SspMI CTAG 2 cut(s) 29, 74
TaaI ACNGT 2 cut(s) 8, 377
TaiI ACGT 1 cut(s) 117
TaqII GACCGA 1 cut(s) 162
TasI AATT 2 cut(s) 256, 286
TatI WGTACW 1 cut(s) 320
TauI GCSGC 2 cut(s) 224, 230
TfiI GAWTC 1 cut(s) 86
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 3 cut(s) 178, 194, 349
TseI GCWGC 5 cut(s) 153, 224, 246, 333, 432
Tsp45I GTSAC 3 cut(s) 178, 194, 349
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 183
Tth111I GACNNNGTC 1 cut(s) 184
XceI RCATGY 1 cut(s) 195
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XspI CTAG 2 cut(s) 29, 74
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.