Rh5AG019300

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000301
Physical Location & Seq
Forward (+)
1362208 .. 1362684
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG019300.1

Sequence Viewer

Length: 477 bp
ATGAAAGGCTCCATCCAAGTCTTCTTTTTGCTAGCCATGCTAATGGCTTTAGCCGCCATTACTCTTTCCGCATCAACCCCGGAGGAAGATCGAGATGAATGGTTCTCTGATGAGGAAACAGATACTCCAAACGAACCATCTGATGATCTCCCTACGACCTTAAGCCAACCAAAAACTTCTCTCAGGGGAGCAAGCCGCTTCCTTGCCACTCGGGCTGTTGCGACTACATGTGACAAAAACCCTAAGGTGTGTAAGGCTGCAGGCAGCGCGGGGCGAGATTGCTGCAAGAAGAAGTGTGTGGATCTGAAGACGGACAGAGTCAATTGCGGCAAGTGTGGGAAGAAATGCAAGTACTCGGAGATATGCTGCAAAGGAAAGTGTCTGAATCCAATGTCTGACAAGAAAAACTGTGGGAGCTGCAACAACAAGTGCAAGAAAGGCAGTTCGTGTGTGTACGGAATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

158

Amino Acids

17.09

Weight (kDa)

8.75

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 75 - 158 1.9e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 269
AciI CCGC 5 cut(s) 54, 69, 196, 269, 327
AclWI GGATC 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 353, 455
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 227
AluBI AGCT 2 cut(s) 417, 468
AluI AGCT 2 cut(s) 417, 468
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 210
ApeKI GCWGC 6 cut(s) 257, 264, 282, 366, 417, 465
AspLEI GCGC 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 80
AsuNHI GCTAGC 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 210
AxyI CCTNAGG 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 13, 314
BbvI GCAGC 5 cut(s) 244, 269, 276, 353, 404
BccI CCATC 2 cut(s) 20, 145
BcgI CGANNNNNNTGC 2 cut(s) 264, 298
BcnI CCSGG 1 cut(s) 80
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 258
BfrI CTTAAG 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
BlsI GCNGC 9 cut(s) 55, 197, 259, 266, 284, 329, 368, 419, 467
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 80
BmtI GCTAGC 1 cut(s) 35
BpiI GAAGAC 2 cut(s) 13, 314
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bse21I CCTNAGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 196
BseXI GCAGC 5 cut(s) 244, 269, 276, 353, 404
Bsh1236I CGCG 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 210
Bsp143I GATC 3 cut(s) 88, 145, 301
BspACI CCGC 5 cut(s) 54, 69, 196, 269, 327
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 10
BspMAI CTGCAG 1 cut(s) 262
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 309
BspTI CTTAAG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 88, 145, 301
Bst4CI ACNGT 1 cut(s) 410
BstAFI CTTAAG 1 cut(s) 160
BstC8I GCNNGC 3 cut(s) 33, 193, 262
BstDEI CTNAG 2 cut(s) 182, 243
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 3 cut(s) 91, 148, 304
BstMBI GATC 3 cut(s) 88, 145, 301
BstMWI GCNNNNNNNGC 5 cut(s) 37, 53, 212, 266, 438
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 258
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 5 cut(s) 244, 269, 276, 353, 404
BstV2I GAAGAC 2 cut(s) 13, 314
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 33, 193, 262
CfoI GCGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 352, 454
CviAII CATG 2 cut(s) 37, 228
CviQI GTAC 2 cut(s) 352, 454
DdeI CTNAG 2 cut(s) 182, 243
DpnI GATC 3 cut(s) 90, 147, 303
DpnII GATC 3 cut(s) 88, 145, 301
Eco57I CTGAAG 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 475
FaeI CATG 2 cut(s) 40, 231
FaiI YATR 5 cut(s) 38, 229, 364, 471, 475
FatI CATG 2 cut(s) 36, 227
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
Fsp4HI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 268
GluI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
HapII CCGG 1 cut(s) 80
HhaI GCGC 1 cut(s) 269
Hin1II CATG 2 cut(s) 40, 231
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 2 cut(s) 318, 385
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 6 cut(s) 109, 142, 306, 358, 384, 397
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 454
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 8 cut(s) 260, 285, 348, 369, 420, 432, 465, 473
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 53, 212, 266, 438
HpyF3I CTNAG 2 cut(s) 182, 243
Hsp92II CATG 2 cut(s) 40, 231
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 3 cut(s) 88, 145, 301
LmnI GCTCC 3 cut(s) 14, 188, 414
LpnPI CCDG 3 cut(s) 93, 169, 246
Lsp1109I GCAGC 5 cut(s) 244, 269, 276, 353, 404
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 90, 147, 303
MboI GATC 3 cut(s) 88, 145, 301
MboII GAAGA 5 cut(s) 13, 98, 301, 319, 352
MfeI CAATTG 1 cut(s) 322
MflI RGATCY 1 cut(s) 301
MluCI AATT 1 cut(s) 322
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 2 cut(s) 76, 106
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 160
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 80
MunI CAATTG 1 cut(s) 322
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 5 cut(s) 37, 53, 212, 266, 438
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 88, 145, 301
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 2 cut(s) 40, 231
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 230
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 231
PciI ACATGT 1 cut(s) 227
PfeI GAWTC 1 cut(s) 385
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 9 cut(s) 55, 197, 259, 266, 284, 329, 368, 419, 467
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PscI ACATGT 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 2 cut(s) 353, 455
RsaNI GTAC 2 cut(s) 352, 454
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 9 cut(s) 54, 196, 258, 265, 283, 328, 367, 418, 466
Sau3AI GATC 3 cut(s) 88, 145, 301
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 80
SetI ASST 4 cut(s) 161, 249, 419, 470
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 322
SsiI CCGC 5 cut(s) 54, 69, 196, 269, 327
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 91
TasI AATT 1 cut(s) 322
TatI WGTACW 1 cut(s) 351
TauI GCSGC 3 cut(s) 56, 198, 330
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 6 cut(s) 257, 264, 282, 366, 417, 465
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 17, 111
TspGWI ACGGA 2 cut(s) 326, 471
Tth111I GACNNNGTC 1 cut(s) 317
Vha464I CTTAAG 1 cut(s) 160
XceI RCATGY 1 cut(s) 231
XspI CTAG 1 cut(s) 32
ZrmI AGTACT 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.