Rh4BG308400

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000301
Physical Location & Seq
Reverse (-)
47764178 .. 47764663
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG308400.1

Sequence Viewer

Length: 486 bp
ATGAAATCCCTCAAGGTTTTCTTATTAGCGGTAGCGATGTTGATGTTTTTAGCCGTTACTCTCTCTGCAACACTAGTCGAAGAAGAACTATTTTCCAACAAGGAAACCAAGGCTGCAAATGATCACGAAACCTTTGATGATGATCTTCCATTGGATGCCAAAAGCCAAGGGAAAACCTCTTTGAGGGGAACAAGCAGCGTCTTCCTTGGTTCTAGGGAAGTGACGACATCATCATGCGACAAAAATCCTAAGGTTTGTAGAGCCGCCGTAAACAGCCCAAAATCACATTGCTGCCACAACAAGTGTGTGGACGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTGAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGTGCACACAATGTCCGACAAGAAACACTGCGGGAGCTGCGACAACCACTGCAAGAGAGGTGGTTCTTGTGCGTTTGGGATGTGCAGCTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

161

Amino Acids

17.41

Weight (kDa)

8.49

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 77 - 161 1.6e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 29, 264, 336, 420
AfiI CCNNNNNNNGG 1 cut(s) 183
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 2 cut(s) 426, 477
AluI AGCT 2 cut(s) 426, 477
Alw21I GWGCWC 1 cut(s) 396
Alw44I GTGCAC 1 cut(s) 392
ApaLI GTGCAC 1 cut(s) 392
ApeKI GCWGC 6 cut(s) 113, 195, 291, 375, 426, 474
AsuHPI GGTGA 1 cut(s) 361
AxyI CCTNAGG 1 cut(s) 249
BaeGI GKGCMC 1 cut(s) 396
BbsI GAAGAC 2 cut(s) 193, 323
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 5 cut(s) 100, 207, 278, 362, 413
BceAI ACGGC 2 cut(s) 38, 251
BclI TGATCA 1 cut(s) 121
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 2 cut(s) 74, 213
BisI GCNGC 7 cut(s) 114, 196, 264, 292, 376, 427, 475
BlsI GCNGC 7 cut(s) 115, 197, 265, 293, 377, 428, 476
BmgBI CACGTC 1 cut(s) 313
BmsI GCATC 1 cut(s) 145
BpiI GAAGAC 2 cut(s) 193, 323
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 108, 166, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse21I CCTNAGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 108, 166, 205
BseGI GGATG 2 cut(s) 160, 474
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 286
BseSI GKGCMC 1 cut(s) 396
BseXI GCAGC 5 cut(s) 100, 207, 278, 362, 413
BsiHKAI GWGCWC 1 cut(s) 396
BslI CCNNNNNNNGG 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 2 cut(s) 121, 142
BspACI CCGC 4 cut(s) 29, 264, 336, 420
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 3 cut(s) 108, 166, 205
BssMI GATC 2 cut(s) 121, 142
BssT1I CCWWGG 3 cut(s) 108, 166, 205
Bst4CI ACNGT 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 359, 363
BstDEI CTNAG 2 cut(s) 249, 483
BstENI CCTNNNNNAGG 1 cut(s) 181
BstF5I GGATG 2 cut(s) 160, 474
BstKTI GATC 2 cut(s) 124, 145
BstMBI GATC 2 cut(s) 121, 142
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 396
BstV1I GCAGC 5 cut(s) 100, 207, 278, 362, 413
BstV2I GAAGAC 2 cut(s) 193, 323
Bsu36I CCTNAGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 160, 474
BtsI GCAGTG 2 cut(s) 415, 436
BtsIMutI CAGTG 2 cut(s) 415, 436
Cac8I GCNNGC 2 cut(s) 359, 363
CseI GACGC 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 2 cut(s) 234, 386
CviJI RGCY 7 cut(s) 53, 113, 165, 263, 276, 426, 477
CviKI_1 RGCY 7 cut(s) 53, 113, 165, 263, 276, 426, 477
DdeI CTNAG 2 cut(s) 249, 483
DpnI GATC 2 cut(s) 123, 144
DpnII GATC 2 cut(s) 121, 142
Eco130I CCWWGG 3 cut(s) 108, 166, 205
Eco81I CCTNAGG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 181
EcoT14I CCWWGG 3 cut(s) 108, 166, 205
ErhI CCWWGG 3 cut(s) 108, 166, 205
FaeI CATG 2 cut(s) 237, 389
FaiI YATR 4 cut(s) 235, 373, 387, 480
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 2 cut(s) 233, 385
FauI CCCGC 2 cut(s) 329, 413
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 7 cut(s) 114, 196, 264, 292, 376, 427, 475
FokI GGATG 2 cut(s) 167, 481
Fsp4HI GCNGC 7 cut(s) 114, 196, 264, 292, 376, 427, 475
FspBI CTAG 2 cut(s) 74, 213
GluI GCNGC 7 cut(s) 114, 196, 264, 292, 376, 427, 475
HgaI GACGC 1 cut(s) 187
Hin1II CATG 2 cut(s) 237, 389
HinfI GANTC 1 cut(s) 327
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 3 cut(s) 271, 310, 394
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 1 cut(s) 125
Hpy8I GTNNAC 3 cut(s) 271, 310, 394
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 68, 116, 357, 378, 394, 441, 474
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 2 cut(s) 249, 483
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 2 cut(s) 237, 389
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 2 cut(s) 121, 142
LmnI GCTCC 1 cut(s) 423
Lsp1109I GCAGC 5 cut(s) 100, 207, 278, 362, 413
LweI GCATC 1 cut(s) 145
MaeI CTAG 2 cut(s) 74, 213
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 55, 220
MalI GATC 2 cut(s) 123, 144
MboI GATC 2 cut(s) 121, 142
MboII GAAGA 5 cut(s) 92, 95, 137, 193, 328
MhlI GDGCHC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 2 cut(s) 120, 429
MnlI CCTC 4 cut(s) 20, 177, 187, 440
MslI CAYNNNNRTG 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 426
NdeII GATC 2 cut(s) 121, 142
NlaIII CATG 2 cut(s) 237, 389
NmuCI GTSAC 1 cut(s) 220
PkrI GCNGC 7 cut(s) 115, 197, 265, 293, 377, 428, 476
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 232
SatI GCNGC 7 cut(s) 114, 196, 264, 292, 376, 427, 475
Sau3AI GATC 2 cut(s) 121, 142
SchI GAGTC 1 cut(s) 321
SduI GDGCHC 1 cut(s) 396
SetI ASST 8 cut(s) 18, 134, 179, 255, 315, 428, 451, 479
SfaNI GCATC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 73
SsiI CCGC 4 cut(s) 29, 264, 336, 420
SspMI CTAG 2 cut(s) 74, 213
StyI CCWWGG 3 cut(s) 108, 166, 205
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 1 cut(s) 315
TaqI TCGA 1 cut(s) 78
TauI GCSGC 1 cut(s) 266
TscAI CASTG 2 cut(s) 422, 443
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 6 cut(s) 113, 195, 291, 375, 426, 474
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 2 cut(s) 422, 443
VneI GTGCAC 1 cut(s) 392
XagI CCTNNNNNAGG 1 cut(s) 181
XspI CTAG 2 cut(s) 74, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.