Rh5BG022400

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000301
Physical Location & Seq
Forward (+)
1644540 .. 1647122
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG022400.1

Sequence Viewer

Length: 678 bp
ATGTTGATGGCTTTAGCCGTTACTCTTTCTGCAACACTAGTCGAAGAAGAACTATTTTCCGACAAGGGAACCGAGGCGACAAATGATCACGAAACCTTTGATGGTGATCTTCCGATGGATGCCAAAAGCCAAGGGAAAACCTCTCTGAGGGGAACAAGTAGTGTCTTCCTTGGTTCTACTTCTAGGGCAGTGACAACATCAACATGCGACAAAAACCCTAAGGTTTGTAGGGCCGCTGCAAACAGCCCAAAATCACATTGCTGCCACAACAAGTGTGTGGATGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTCAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGCATGTGCACACAATGTCCGACAAGAAACACTGCGGGAGCTGCGACAACCATTGCAAGCGAGGTGGTTCTTGTGCGTTTGGGATGTGCAGCTATGCTTAGGAATTATGAAGCTAGTTTGTGTACTACTTCTACTCATTACTCAATATGGTTCACAATTGGATGTAGAGGATTTGGAGCAACTCAGGACGACACCACTACTAGCTGGTTGCAACAACTGGGATCAAGAAATACAACACCTTAAATTTTGGCCAGACACTCTGAGCTCGTTCAAGAACATAGCGGACGAATTGGAACAACTTGAGGCCATCCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

225

Amino Acids

24.94

Weight (kDa)

5.8

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 67 - 121 3.9e-12 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 234, 306, 394, 641
AclWI GGATC 1 cut(s) 588
AcoI YGGCCR 1 cut(s) 608
AcsI RAATTY 1 cut(s) 602
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 1 cut(s) 631
AhlI ACTAGT 1 cut(s) 37
AluBI AGCT 6 cut(s) 400, 451, 472, 563, 624, 674
AluI AGCT 6 cut(s) 400, 451, 472, 563, 624, 674
Alw21I GWGCWC 2 cut(s) 370, 626
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 1 cut(s) 588
AoxI GGCC 3 cut(s) 231, 608, 663
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 5 cut(s) 236, 261, 345, 400, 448
ApoI RAATTY 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 116
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 370
BalI TGGCCA 1 cut(s) 610
BanII GRGCYC 1 cut(s) 626
BbsI GAAGAC 2 cut(s) 157, 293
Bbv12I GWGCWC 2 cut(s) 370, 626
BbvI GCAGC 5 cut(s) 223, 248, 332, 387, 460
BccI CCATC 3 cut(s) 95, 109, 674
BceAI ACGGC 1 cut(s) 2
BclI TGATCA 1 cut(s) 85
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 4 cut(s) 38, 183, 473, 560
BisI GCNGC 6 cut(s) 234, 237, 262, 346, 401, 449
BlsI GCNGC 6 cut(s) 235, 238, 263, 347, 402, 450
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 70
BmrI ACTGGG 1 cut(s) 586
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 586
BpiI GAAGAC 2 cut(s) 157, 293
Bpu10I CCTNAGC 1 cut(s) 457
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 72, 130, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 581
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 256, 410
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 72, 130, 169
BseGI GGATG 5 cut(s) 124, 286, 448, 526, 666
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 2 cut(s) 256, 410
BseMII CTCAG 3 cut(s) 137, 556, 611
BseNI ACTGG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 5 cut(s) 223, 248, 332, 387, 460
BsgI GTGCAG 1 cut(s) 467
BshFI GGCC 3 cut(s) 233, 610, 665
BsiHKAI GWGCWC 2 cut(s) 370, 626
BslI CCNNNNNNNGG 1 cut(s) 147
BsnI GGCC 3 cut(s) 233, 610, 665
Bsp1286I GDGCHC 2 cut(s) 370, 626
Bsp143I GATC 3 cut(s) 85, 106, 580
BspACI CCGC 4 cut(s) 234, 306, 394, 641
BspANI GGCC 3 cut(s) 233, 610, 665
BspCNI CTCAG 3 cut(s) 138, 555, 612
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 1 cut(s) 588
BsrDI GCAATG 2 cut(s) 256, 410
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 3 cut(s) 72, 130, 169
BssMI GATC 3 cut(s) 85, 106, 580
BssT1I CCWWGG 2 cut(s) 130, 169
Bst4CI ACNGT 1 cut(s) 353
BstC8I GCNNGC 3 cut(s) 329, 333, 417
BstDEI CTNAG 5 cut(s) 146, 219, 457, 542, 620
BstENI CCTNNNNNAGG 1 cut(s) 145
BstF5I GGATG 5 cut(s) 124, 286, 448, 526, 666
BstKTI GATC 3 cut(s) 88, 109, 583
BstMBI GATC 3 cut(s) 85, 106, 580
BstMWI GCNNNNNNNGC 2 cut(s) 400, 671
BstNSI RCATGY 2 cut(s) 207, 366
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 5 cut(s) 223, 248, 332, 387, 460
BstV2I GAAGAC 2 cut(s) 157, 293
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 3 cut(s) 233, 610, 665
BtsCI GGATG 5 cut(s) 124, 286, 448, 526, 666
BtsI GCAGTG 2 cut(s) 195, 389
BtsIMutI CAGTG 2 cut(s) 195, 389
Cac8I GCNNGC 3 cut(s) 329, 333, 417
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 482
CspCI CAANNNNNGTGG 2 cut(s) 404, 439
CviAII CATG 3 cut(s) 204, 356, 363
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 5 cut(s) 146, 219, 457, 542, 620
DpnI GATC 3 cut(s) 87, 108, 582
DpnII GATC 3 cut(s) 85, 106, 580
EaeI YGGCCR 1 cut(s) 608
Ecl136II GAGCTC 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 130, 169
Eco24I GRGCYC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 624
Eco81I CCTNAGG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 624
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 130, 169
EcoT38I GRGCYC 1 cut(s) 626
ErhI CCWWGG 2 cut(s) 130, 169
FaeI CATG 3 cut(s) 207, 359, 366
FaiI YATR 8 cut(s) 205, 343, 357, 364, 454, 467, 507, 638
FatI CATG 3 cut(s) 203, 355, 362
FauI CCCGC 2 cut(s) 299, 387
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 6 cut(s) 234, 237, 262, 346, 401, 449
FokI GGATG 5 cut(s) 131, 293, 455, 533, 653
FriOI GRGCYC 1 cut(s) 626
Fsp4HI GCNGC 6 cut(s) 234, 237, 262, 346, 401, 449
FspBI CTAG 4 cut(s) 38, 183, 473, 560
GluI GCNGC 6 cut(s) 234, 237, 262, 346, 401, 449
HaeIII GGCC 3 cut(s) 233, 610, 665
Hin1II CATG 3 cut(s) 207, 359, 366
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 1 cut(s) 297
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 3 cut(s) 368, 482, 512
Hpy188I TCNGA 5 cut(s) 61, 114, 147, 380, 621
Hpy188III TCNNGA 4 cut(s) 89, 544, 584, 631
Hpy8I GTNNAC 3 cut(s) 368, 482, 512
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4V TGCA 9 cut(s) 32, 239, 327, 348, 362, 368, 415, 448, 570
HpyF10VI GCNNNNNNNGC 2 cut(s) 400, 671
HpyF3I CTNAG 5 cut(s) 146, 219, 457, 542, 620
Hsp92II CATG 3 cut(s) 207, 359, 366
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 3 cut(s) 85, 106, 580
LmnI GCTCC 2 cut(s) 397, 535
LpnPI CCDG 4 cut(s) 529, 549, 562, 624
Lsp1109I GCAGC 5 cut(s) 223, 248, 332, 387, 460
LweI GCATC 1 cut(s) 109
MaeI CTAG 4 cut(s) 38, 183, 473, 560
MaeIII GTNAC 2 cut(s) 19, 190
MalI GATC 3 cut(s) 87, 108, 582
MboI GATC 3 cut(s) 85, 106, 580
MboII GAAGA 5 cut(s) 56, 59, 101, 157, 298
MfeI CAATTG 1 cut(s) 515
MhlI GDGCHC 2 cut(s) 370, 626
MlsI TGGCCA 1 cut(s) 610
MluCI AATT 4 cut(s) 462, 515, 602, 647
MluNI TGGCCA 1 cut(s) 610
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 2 cut(s) 84, 403
MnlI CCTC 6 cut(s) 67, 141, 151, 414, 520, 655
Mox20I TGGCCA 1 cut(s) 610
MscI TGGCCA 1 cut(s) 610
MseI TTAA 1 cut(s) 600
MslI CAYNNNNRTG 1 cut(s) 202
Msp20I TGGCCA 1 cut(s) 610
MspA1I CMGCKG 1 cut(s) 236
MunI CAATTG 1 cut(s) 515
MwoI GCNNNNNNNGC 2 cut(s) 400, 671
NdeII GATC 3 cut(s) 85, 106, 580
NlaIII CATG 3 cut(s) 207, 359, 366
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 2 cut(s) 207, 366
PkrI GCNGC 6 cut(s) 235, 238, 263, 347, 402, 450
PleI GAGTC 1 cut(s) 291
PpsI GAGTC 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 626
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 231
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 202
SacI GAGCTC 1 cut(s) 626
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 6 cut(s) 234, 237, 262, 346, 401, 449
Sau3AI GATC 3 cut(s) 85, 106, 580
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 291
SduI GDGCHC 2 cut(s) 370, 626
SfaNI GCATC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 4 cut(s) 462, 515, 602, 647
SsiI CCGC 4 cut(s) 234, 306, 394, 641
SspMI CTAG 4 cut(s) 38, 183, 473, 560
SstI GAGCTC 1 cut(s) 626
StyI CCWWGG 2 cut(s) 130, 169
TaaI ACNGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 42
TasI AATT 4 cut(s) 462, 515, 602, 647
TatI WGTACW 1 cut(s) 481
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 2 cut(s) 195, 396
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 5 cut(s) 236, 261, 345, 400, 448
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 482
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 195, 396
VneI GTGCAC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 1 cut(s) 602
XceI RCATGY 2 cut(s) 207, 366
XspI CTAG 4 cut(s) 38, 183, 473, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.