Rroxscaffold_1G00039860

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000301
Physical Location & Seq
Forward (+)
57538340 .. 57538762
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039860.1

Sequence Viewer

Length: 423 bp
ATGAATATCGAAGCAAAGCCCACTATACAGGAAGAGAAAGATGGTCATCAAGAAGTCGCCATTGATGATTCTCTGAACTCTCCTGACAATGAAACTGAATCAAATTCCTTCTTCTTACACAGAAATGACCGCTCTCTCAGGCAAAAGATGAGAACACATACGGTGCGGTTGACCTGTAGCAAGTTCCCCAGGATATGTTATACTAAGGGAAGCCCAGGACCTCATTGCTGCAAGATGAAGTGTGTTAATGTTTTAACAGACAAGCTTAATTGTGGGAAATGTGGAAAGAAATGCAAGTACAATGAGGTTTGCTGCAAAGGGAAATGTGTAAATCCATCTTTCAACCGGAGTCATTGTGGTGGATGTAACAGTAGGTGCAAAGATGGAGGGTTTTGTGCATTCGGCATATGCAACTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

140

Amino Acids

15.67

Weight (kDa)

8.87

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 58 - 140 7.5e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 132
AciI CCGC 2 cut(s) 130, 166
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 1 cut(s) 299
AgsI TTSAA 1 cut(s) 343
AjnI CCWGG 2 cut(s) 188, 214
AluBI AGCT 1 cut(s) 265
AluI AGCT 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 228, 312
ApoI RAATTY 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 218
AvaII GGWCC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 215, 299
BccI CCATC 3 cut(s) 35, 343, 377
BciT130I CCWGG 2 cut(s) 190, 216
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 2 cut(s) 229, 313
BlsI GCNGC 2 cut(s) 230, 314
Bme1390I CCNGG 2 cut(s) 190, 216
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 190, 216
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 188, 214
BsaWI WCCGGW 1 cut(s) 345
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bse3DI GCAATG 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 190, 216
BseDI CCNNGG 2 cut(s) 188, 214
BseGI GGATG 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 45
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 215, 299
BsiSI CCGG 1 cut(s) 346
BsmI GAATGC 1 cut(s) 398
BspACI CCGC 2 cut(s) 130, 166
BspCNI CTCAG 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 188, 214
Bst2UI CCWGG 2 cut(s) 190, 216
Bst4CI ACNGT 2 cut(s) 163, 371
Bst6I CTCTTC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 137, 204
BstF5I GGATG 1 cut(s) 368
BstNI CCWGG 2 cut(s) 190, 216
BstSCI CCNGG 2 cut(s) 188, 214
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 215, 299
BtsCI GGATG 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 298
CviJI RGCY 3 cut(s) 19, 213, 265
CviKI_1 RGCY 3 cut(s) 19, 213, 265
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 2 cut(s) 137, 204
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 188, 214
EcoT22I ATGCAT 1 cut(s) 421
FaiI YATR 8 cut(s) 26, 159, 196, 201, 407, 409, 417, 421
FauNDI CATATG 1 cut(s) 407
Fnu4HI GCNGC 2 cut(s) 229, 313
FokI GGATG 1 cut(s) 375
Fsp4HI GCNGC 2 cut(s) 229, 313
GluI GCNGC 2 cut(s) 229, 313
HapII CCGG 1 cut(s) 346
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 68, 98, 349
HpaII CCGG 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 2 cut(s) 50, 83
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 2 cut(s) 163, 371
HpyCH4V TGCA 7 cut(s) 231, 294, 315, 378, 398, 411, 419
HpyF3I CTNAG 2 cut(s) 137, 204
LpnPI CCDG 9 cut(s) 14, 96, 124, 175, 187, 201, 202, 228, 359
Lsp1109I GCAGC 2 cut(s) 215, 299
MaeIII GTNAC 1 cut(s) 365
MbiI CCGCTC 1 cut(s) 132
MboII GAAGA 2 cut(s) 44, 103
MluCI AATT 2 cut(s) 103, 268
MlyI GAGTC 1 cut(s) 358
MnlI CCTC 3 cut(s) 231, 298, 380
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 3 cut(s) 246, 254, 267
MslI CAYNNNNRTG 2 cut(s) 123, 357
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 2 cut(s) 190, 216
Mva1269I GAATGC 1 cut(s) 398
MvaI CCWGG 2 cut(s) 190, 216
NdeI CATATG 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 421
PctI GAATGC 1 cut(s) 398
PfeI GAWTC 2 cut(s) 68, 98
PkrI GCNGC 2 cut(s) 230, 314
PleI GAGTC 1 cut(s) 357
PpsI GAGTC 1 cut(s) 357
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 188, 214
PspGI CCWGG 2 cut(s) 188, 214
PspPI GGNCC 1 cut(s) 218
PspPPI RGGWCCY 1 cut(s) 218
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 2 cut(s) 123, 357
SaqAI TTAA 3 cut(s) 246, 254, 267
SatI GCNGC 2 cut(s) 229, 313
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 190, 216
SetI ASST 5 cut(s) 176, 223, 267, 309, 377
SfcI CTRYAG 1 cut(s) 175
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 123, 357
Sse9I AATT 2 cut(s) 103, 268
SsiI CCGC 2 cut(s) 130, 166
StyD4I CCNGG 2 cut(s) 188, 214
TaaI ACNGT 2 cut(s) 163, 371
TaqI TCGA 1 cut(s) 9
TasI AATT 2 cut(s) 103, 268
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 2 cut(s) 68, 98
Tru1I TTAA 3 cut(s) 246, 254, 267
Tru9I TTAA 3 cut(s) 246, 254, 267
TseI GCWGC 2 cut(s) 228, 312
TspDTI ATGAA 3 cut(s) 17, 105, 251
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 1 cut(s) 103
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.