pycom05g31780

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000301
Physical Location & Seq
Reverse (-)
31383993 .. 31384430
438 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31780.1

Sequence Viewer

Length: 438 bp
ATGGCCATGAAACTGCCCAAGTTTTTGTTCACATTGGTGTTGATAGCGGGCGTAGTCATTTTCTCCTCAGCAACTCCAACTATTGAAGAGACACATGGTAACACCAAATCACAAAACCATGGTAGTTCTATCAGTCGGTTTCTTGGTTTGCACGAGTTTGTAGGGCATCGACATCGACCAAGGATAACTTGTGAGAGGAGTCCAAGAATTTGCTGGGCAATGGGCAGCCCAGGACCTTACTGCTGCAACAAAAAATGCGTCAATGTGATGACTGATAAACATAACTGTGGAATGTGTGGAAGAAAGTGTAACTATTCAGGACTGTGCTGCAAAGGGAGTTGTGTGTATCCCTCTGTGGATGAGAGACATTGTGGCAAGTGCGACAACAGGTGCCCAGCGGGAAGCTTATGCGTCTATGGGCTCTGCAGTTATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

146

Amino Acids

15.93

Weight (kDa)

8.98

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 62 - 145 1.1e-29 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 390
AciI CCGC 2 cut(s) 47, 398
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 207
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 1 cut(s) 229
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 1 cut(s) 405
AluI AGCT 1 cut(s) 405
Alw26I GTCTC 2 cut(s) 83, 358
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 225, 243, 327
ApoI RAATTY 1 cut(s) 207
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspS9I GGNCC 1 cut(s) 233
AvaII GGWCC 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 423
BauI CACGAG 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 230, 237, 314
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 83, 358
BfmI CTRYAG 1 cut(s) 424
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 3 cut(s) 226, 244, 328
BlsI GCNGC 3 cut(s) 227, 245, 329
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 118, 179, 229
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 118, 179, 229
BseGI GGATG 1 cut(s) 364
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 81
BseRI GAGGAG 2 cut(s) 55, 211
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 230, 237, 314
BseYI CCCAGC 2 cut(s) 213, 394
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 390
BsmAI GTCTC 2 cut(s) 83, 358
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 395, 423
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 2 cut(s) 47, 398
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 392
BspMAI CTGCAG 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 225
BssECI CCNNGG 3 cut(s) 118, 179, 229
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 118, 179
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 2 cut(s) 287, 324
Bst6I CTCTTC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 364
BstMAI GTCTC 2 cut(s) 83, 358
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 424
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 230, 237, 314
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 2 cut(s) 247, 400
CviAII CATG 3 cut(s) 7, 95, 119
CviJI RGCY 4 cut(s) 5, 228, 405, 421
CviKI_1 RGCY 4 cut(s) 5, 228, 405, 421
DdeI CTNAG 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 118, 179
Eco24I GRGCYC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 118, 179
EcoT38I GRGCYC 1 cut(s) 423
ErhI CCWWGG 2 cut(s) 118, 179
FaeI CATG 3 cut(s) 10, 98, 122
FaiI YATR 7 cut(s) 8, 96, 120, 282, 409, 417, 432
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 3 cut(s) 6, 94, 118
FauI CCCGC 2 cut(s) 40, 391
Fnu4HI GCNGC 3 cut(s) 226, 244, 328
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 3 cut(s) 226, 244, 328
GluI GCNGC 3 cut(s) 226, 244, 328
GsaI CCCAGC 2 cut(s) 217, 398
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 247, 400
Hin1II CATG 3 cut(s) 10, 98, 122
HindIII AAGCTT 1 cut(s) 403
HinfI GANTC 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 30
Hpy188III TCNNGA 1 cut(s) 318
Hpy8I GTNNAC 1 cut(s) 30
HpyCH4III ACNGT 2 cut(s) 287, 324
HpyCH4V TGCA 4 cut(s) 151, 246, 330, 426
HpyF3I CTNAG 1 cut(s) 67
Hsp92II CATG 3 cut(s) 10, 98, 122
LpnPI CCDG 6 cut(s) 199, 216, 243, 303, 373, 408
Lsp1109I GCAGC 3 cut(s) 230, 237, 314
LweI GCATC 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 98, 308
MboII GAAGA 2 cut(s) 98, 312
MhlI GDGCHC 2 cut(s) 395, 423
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 3 cut(s) 76, 189, 361
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 436
MslI CAYNNNNRTG 2 cut(s) 35, 285
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 231
NcoI CCATGG 1 cut(s) 118
NlaIII CATG 3 cut(s) 10, 98, 122
NlaIV GGNNCC 1 cut(s) 392
OliI CACNNNNGTG 1 cut(s) 35
PkrI GCNGC 3 cut(s) 227, 245, 329
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 2 cut(s) 213, 394
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 1 cut(s) 233
PspPPI RGGWCCY 1 cut(s) 233
PstI CTGCAG 1 cut(s) 428
RseI CAYNNNNRTG 2 cut(s) 35, 285
SaqAI TTAA 1 cut(s) 436
SatI GCNGC 3 cut(s) 226, 244, 328
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 395, 423
SetI ASST 3 cut(s) 238, 392, 407
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 2 cut(s) 35, 285
Sse9I AATT 1 cut(s) 207
SsiI CCGC 2 cut(s) 47, 398
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 2 cut(s) 118, 179
TaaI ACNGT 2 cut(s) 287, 324
TaqI TCGA 2 cut(s) 169, 175
TasI AATT 1 cut(s) 207
Tru1I TTAA 1 cut(s) 436
Tru9I TTAA 1 cut(s) 436
TseI GCWGC 3 cut(s) 225, 243, 327
TspDTI ATGAA 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 1 cut(s) 207
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.