Rroxscaffold_1G00073380

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000301
Physical Location & Seq
Reverse (-)
94260239 .. 94273428
13190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073380.1

Sequence Viewer

Length: 465 bp
ATGAATTTTTCAAGGTTTTGCTTATTAGTGGTAGCGATGTTGATGGCTTTAGCCGTTACTCTTTCTGCAACACTAGTCGAAGAAGAACTATTTTCCGACAAGGGAACCGAGGCTACAAATGATCACGAAACCTTTGATGTCCAAAACCAAGAGAAAACTTCTCTCAGGGGAACAAGCAGCGTCTTCTTTGCTTCTCGGTCAGTGACAGCGTCAACATGTGACAAAAACCCGAAGGTTTGTGGCGCGGCTGCCGCGGACGGCTCGGAGTGCTGCAGTAAGAAGTGCGTGGATTTGAAGACGGACAGACTCAATTGCGGGAAATGTGGGGTGAAATGCAAGCCGACAGAGATATGCTGCAATGGTCATATTGTGACTCCAATGTCTGACAAGAAAAACTGTGGGAGATGCAACAATGCTTGCAAGGGACGCAACTCATGCGCGTTTGGGATGTGCAGCTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

154

Amino Acids

16.48

Weight (kDa)

8.13

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 71 - 154 4.5e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 379
AccII CGCG 3 cut(s) 245, 254, 440
AciI CCGC 4 cut(s) 245, 252, 254, 315
AcsI RAATTY 1 cut(s) 4
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 2 cut(s) 12, 295
AhlI ACTAGT 1 cut(s) 73
AluBI AGCT 1 cut(s) 456
AluI AGCT 1 cut(s) 456
ApeKI GCWGC 5 cut(s) 177, 248, 270, 354, 453
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 2 cut(s) 245, 440
AsuHPI GGTGA 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 175, 302
BbvI GCAGC 4 cut(s) 189, 235, 257, 341
BccI CCATC 1 cut(s) 37
BceAI ACGGC 2 cut(s) 38, 274
BclI TGATCA 1 cut(s) 121
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 7 cut(s) 178, 246, 249, 252, 271, 355, 454
BlsI GCNGC 7 cut(s) 179, 247, 250, 253, 272, 356, 455
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 2 cut(s) 175, 302
BsaJI CCNNGG 2 cut(s) 108, 252
Bse3DI GCAATG 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 108, 252
BseGI GGATG 1 cut(s) 453
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 1 cut(s) 178
BseXI GCAGC 4 cut(s) 189, 235, 257, 341
Bsh1236I CGCG 3 cut(s) 245, 254, 440
BslFI GGGAC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 438
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 4 cut(s) 245, 252, 254, 315
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 3 cut(s) 245, 254, 440
BspLI GGNNCC 1 cut(s) 106
BspMAI CTGCAG 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 364
BssECI CCNNGG 2 cut(s) 108, 252
BssMI GATC 1 cut(s) 121
Bst4CI ACNGT 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 435
BstC8I GCNNGC 2 cut(s) 338, 418
BstDEI CTNAG 1 cut(s) 164
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 3 cut(s) 245, 254, 440
BstHHI GCGC 2 cut(s) 245, 440
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 4 cut(s) 251, 267, 426, 435
BstNSI RCATGY 1 cut(s) 219
BstSFI CTRYAG 1 cut(s) 271
BstUI CGCG 3 cut(s) 245, 254, 440
BstV1I GCAGC 4 cut(s) 189, 235, 257, 341
BstV2I GAAGAC 2 cut(s) 175, 302
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 338, 418
CfoI GCGC 2 cut(s) 245, 440
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 3 cut(s) 169, 198, 435
CviAII CATG 2 cut(s) 216, 435
CviJI RGCY 7 cut(s) 47, 53, 113, 248, 261, 340, 456
CviKI_1 RGCY 7 cut(s) 47, 53, 113, 248, 261, 340, 456
DdeI CTNAG 1 cut(s) 164
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
DrdI GACNNNNNNGTC 1 cut(s) 379
DseDI GACNNNNNNGTC 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 463
FaeI CATG 2 cut(s) 219, 438
FaiI YATR 6 cut(s) 217, 352, 366, 436, 459, 463
FaqI GGGAC 1 cut(s) 438
FatI CATG 2 cut(s) 215, 434
FauI CCCGC 1 cut(s) 308
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 7 cut(s) 178, 246, 249, 252, 271, 355, 454
FokI GGATG 1 cut(s) 460
Fsp4HI GCNGC 7 cut(s) 178, 246, 249, 252, 271, 355, 454
FspBI CTAG 1 cut(s) 74
GlaI GCGC 2 cut(s) 244, 439
GluI GCNGC 7 cut(s) 178, 246, 249, 252, 271, 355, 454
HgaI GACGC 3 cut(s) 169, 198, 435
HhaI GCGC 2 cut(s) 245, 440
Hin1II CATG 2 cut(s) 219, 438
Hin6I GCGC 2 cut(s) 243, 438
HinP1I GCGC 2 cut(s) 243, 438
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 2 cut(s) 306, 373
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 3 cut(s) 97, 265, 385
Hpy188III TCNNGA 1 cut(s) 125
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4V TGCA 8 cut(s) 68, 273, 336, 357, 408, 420, 453, 461
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 267, 426, 435
HpyF3I CTNAG 1 cut(s) 164
Hsp92II CATG 2 cut(s) 219, 438
HspAI GCGC 2 cut(s) 243, 438
Ksp22I TGATCA 1 cut(s) 121
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 1 cut(s) 121
LpnPI CCDG 1 cut(s) 151
Lsp1109I GCAGC 4 cut(s) 189, 235, 257, 341
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 74
MaeIII GTNAC 4 cut(s) 55, 202, 218, 370
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 4 cut(s) 92, 95, 175, 307
MfeI CAATTG 1 cut(s) 310
MluCI AATT 2 cut(s) 4, 310
MlyI GAGTC 2 cut(s) 300, 367
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 1 cut(s) 103
Mph1103I ATGCAT 1 cut(s) 463
MspA1I CMGCKG 1 cut(s) 254
MunI CAATTG 1 cut(s) 310
MvnI CGCG 3 cut(s) 245, 254, 440
MwoI GCNNNNNNNGC 4 cut(s) 251, 267, 426, 435
NdeII GATC 1 cut(s) 121
NlaIII CATG 2 cut(s) 219, 438
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 3 cut(s) 202, 218, 370
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 219
PciI ACATGT 1 cut(s) 215
PflFI GACNNNGTC 1 cut(s) 208
PkrI GCNGC 7 cut(s) 179, 247, 250, 253, 272, 356, 455
PleI GAGTC 2 cut(s) 300, 367
PpsI GAGTC 2 cut(s) 300, 367
PscI ACATGT 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 106
PsrI GAACNNNNNNTAC 2 cut(s) 97, 129
PstI CTGCAG 1 cut(s) 275
PsyI GACNNNGTC 1 cut(s) 208
SacII CCGCGG 1 cut(s) 255
SatI GCNGC 7 cut(s) 178, 246, 249, 252, 271, 355, 454
Sau3AI GATC 1 cut(s) 121
SchI GAGTC 2 cut(s) 300, 367
SetI ASST 4 cut(s) 17, 134, 237, 458
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 271
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 73
Sse9I AATT 2 cut(s) 4, 310
SsiI CCGC 4 cut(s) 245, 252, 254, 315
SspMI CTAG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 398
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 186
TasI AATT 2 cut(s) 4, 310
TauI GCSGC 2 cut(s) 248, 254
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 3 cut(s) 202, 218, 370
TseI GCWGC 5 cut(s) 177, 248, 270, 354, 453
Tsp45I GTSAC 3 cut(s) 202, 218, 370
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 207
Tth111I GACNNNGTC 1 cut(s) 208
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 219
XspI CTAG 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.