Rmu_co8419035.1_g000001

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8419035.1
OG0000301
Physical Location & Seq
Reverse (-)
707 .. 1291
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8419035.1_g000001.1.cds

Sequence Viewer

Length: 585 bp
atggctgttgtttcagctgcgtttgatctggaagattacactgaagaaccggaagtgcagagtaatgaatcaacggtgcctgaatctgaaatgcaagaagcgactacttctttgagaggagtgagccgctttctgacttcccagcagaacctgttgggaaacttgacctgcaacaagtttcctagggtttgtcgtctgaagaagagcccagggcccgactgctgcaagaagaagtgtgtcaatgtgaagacggataaattgaactgcgggttctgcgggcgcaagtgcaagcaaactgggatttgttgcaaggggaagtgcgtcaatgtgaagacggataaattgaactgcggggtctgcggttacaaatgcaagtaccctgagatttgttgcaagggaaagtgcgtcaatgtgaagaaggataaattgaactgcgggatctgcggttacaagtgcaagtaccctgagatttgctgcaaggggaagtgcgtcaatccaacttgtgacaaaaagaattgcggtggatgcaacaagaagtgcaagagaggggagttatgtgtttatgggatgtgcaattatgcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

194

Amino Acids

21.37

Weight (kDa)

8.96

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11925
fragaria_vesca FvH4_2g01120 FvH4_3g01640 FvH4_3g01660 FvH4_3g01670 FvH4_3g01672 FvH4_3g18030
malus_domestica MD05G1350300.v1.1 MD05G1350400.v1.1 MD05G1350500.v1.1 MD07G1060200.v1.1 MD07G1060300.v1.1 MD10G1325000.v1.1 MD10G1325100.v1.1 MD10G1325200.v1.1 MD10G1325300.v1.1 MD11G1212900.v1.1
prunus_persica Prupe.4G016900_v2.0.a1 Prupe.4G017000_v2.0.a1 Prupe.4G017100_v2.0.a1 Prupe.4G017200_v2.0.a1 Prupe.4G020300_v2.0.a1 Prupe.4G218500_v2.0.a1 Prupe.4G218600_v2.0.a1
pyrus_communis pycom05g31780 pycom05g31790
rosa_chinensis RchiOBHm_Chr4g0430681 RchiOBHm_Chr4g0430691 RchiOBHm_Chr5g0002431 RchiOBHm_Chr5g0002441 RchiOBHm_Chr5g0002451 RchiOBHm_Chr5g0002461 RchiOBHm_Chr5g0002511 RchiOBHm_Chr5g0002521 RchiOBHm_Chr5g0030071 RchiOBHm_Chr5g0040841
rosa_laevigata RLG00000006955 RLG00000006956 RLG00000031032 RLG00000031033 RLG00000031035 RLG00000031036
rosa_multiflora Rmu_co8168770.1_g000001 Rmu_co8419035.1_g000001 Rmu_co8421345.1_g000001 Rmu_sc0000131.1_g000010 Rmu_sc0000131.1_g000011 Rmu_sc0000140.1_g000002 Rmu_sc0001717.1_g000006 Rmu_sc0001717.1_g000007 Rmu_sc0001717.1_g000008 Rmu_sc0001717.1_g000012 Rmu_sc0003568.1_g000001 Rmu_sc0013444.1_g000002 Rmu_sc0039536.1_g000001
rosa_roxburghii Rroxscaffold_1G00039860 Rroxscaffold_1G00049920 Rroxscaffold_1G00049960 Rroxscaffold_1G00073380 Rroxscaffold_1G00073390 Rroxscaffold_1G00073410 Rroxscaffold_1G00073420 Rroxscaffold_5G00372420 Rroxscaffold_5G00372430
rosa_rugosa Rorug04G0245100 Rorug04G0245200 Rorug04G0396600 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug04G0396700 Rorug05G0116800 Rorug05G0188100 Rorug05G0220100
rosa_samantha Rh4BG308400 Rh4BG308500 Rh4BG308800 Rh4BG308900 Rh4BG309100 Rh4BG309200 Rh4CG325600 Rh4CG325700 Rh4DG306000 Rh4DG306100 Rh5AG019100 Rh5AG019200 Rh5AG019300 Rh5AG019600 Rh5AG019700 Rh5AG020000 Rh5BG022000 Rh5BG022100 Rh5BG022300 Rh5BG022400 Rh5BG022700 Rh5CG021000 Rh5CG021100 Rh5CG021200 Rh5CG021500 Rh5CG021600 Rh5CG022000 Rh5CG232500 Rh5CG310700 Rh5DG020100 Rh5DG020300 Rh5DG020400 Rh5DG020600 Rh5DG020800 Rh5DG021100 Rh5DG213100 Rh5DG286900
rosa_wichuraiana Rw4G026160 Rw4G026170 Rw5G001790 Rw5G001800 Rw5G001810 Rw5G001820 Rw5G001840 Rw5G019110 Rw5G025720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 8 cut(s) 127, 267, 276, 351, 360, 435, 444, 519
AclWI GGATC 1 cut(s) 446
AcuI CTGAAG 2 cut(s) 63, 218
AfaI GTAC 2 cut(s) 377, 461
AgsI TTSAA 3 cut(s) 262, 346, 430
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
AlwI GGATC 1 cut(s) 446
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 1 cut(s) 212
ApaI GGGCCC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 17, 222, 474
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AspA2I CCTAGG 1 cut(s) 182
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 2 cut(s) 212, 213
AvrII CCTAGG 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 216
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 209, 216
BbsI GAAGAC 2 cut(s) 254, 338
BbvI GCAGC 3 cut(s) 4, 209, 461
BciT130I CCWGG 1 cut(s) 210
BfaI CTAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 4 cut(s) 18, 127, 223, 475
BlnI CCTAGG 1 cut(s) 182
BlsI GCNGC 4 cut(s) 19, 128, 224, 476
Bme1390I CCNGG 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 212, 213
BmiI GGNNCC 2 cut(s) 78, 214
BmrFI CCNGG 1 cut(s) 210
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 515
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 254, 338
BsaJI CCNNGG 3 cut(s) 182, 208, 209
BsaWI WCCGGW 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 182, 208, 209
BseGI GGATG 2 cut(s) 530, 573
BseMII CTCAG 2 cut(s) 372, 456
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 3 cut(s) 4, 209, 461
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 76
BsiSI CCGG 1 cut(s) 50
BsnI GGCC 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 209, 216
Bsp143I GATC 2 cut(s) 25, 438
BspACI CCGC 8 cut(s) 127, 267, 276, 351, 360, 435, 444, 519
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 2 cut(s) 373, 457
BspLI GGNNCC 2 cut(s) 78, 214
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 446
BspQI GCTCTTC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 3 cut(s) 182, 208, 209
BssMI GATC 2 cut(s) 25, 438
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 278, 290
BstDEI CTNAG 2 cut(s) 381, 465
BstF5I GGATG 2 cut(s) 530, 573
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 2 cut(s) 28, 441
BstMBI GATC 2 cut(s) 25, 438
BstMWI GCNNNNNNNGC 4 cut(s) 273, 357, 441, 525
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 4, 209, 461
BstV2I GAAGAC 2 cut(s) 254, 338
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 530, 573
BtsIMutI CAGTG 1 cut(s) 39
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 278, 290
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 212, 213
CseI GACGC 3 cut(s) 310, 394, 478
Csp6I GTAC 2 cut(s) 376, 460
CviAII CATG 1 cut(s) 582
CviJI RGCY 5 cut(s) 5, 17, 126, 207, 214
CviKI_1 RGCY 5 cut(s) 5, 17, 126, 207, 214
CviQI GTAC 2 cut(s) 376, 460
DdeI CTNAG 2 cut(s) 381, 465
DpnI GATC 2 cut(s) 27, 440
DpnII GATC 2 cut(s) 25, 438
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 2 cut(s) 209, 216
Eco57I CTGAAG 2 cut(s) 63, 218
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 2 cut(s) 209, 216
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 1 cut(s) 585
FaiI YATR 4 cut(s) 556, 564, 579, 583
FatI CATG 1 cut(s) 581
FauI CCCGC 4 cut(s) 260, 269, 344, 428
Fnu4HI GCNGC 4 cut(s) 18, 127, 223, 475
FokI GGATG 2 cut(s) 537, 580
FriOI GRGCYC 2 cut(s) 209, 216
Fsp4HI GCNGC 4 cut(s) 18, 127, 223, 475
FspBI CTAG 1 cut(s) 183
GlaI GCGC 1 cut(s) 281
GluI GCNGC 4 cut(s) 18, 127, 223, 475
GsaI CCCAGC 1 cut(s) 145
HaeIII GGCC 1 cut(s) 214
HapII CCGG 1 cut(s) 50
HgaI GACGC 3 cut(s) 310, 394, 478
HhaI GCGC 1 cut(s) 282
Hin1II CATG 1 cut(s) 585
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 2 cut(s) 68, 83
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 3 cut(s) 88, 135, 198
Hpy188III TCNNGA 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 357, 441, 525
HpyF3I CTNAG 2 cut(s) 381, 465
Hsp92II CATG 1 cut(s) 585
HspAI GCGC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 25, 438
LguI GCTCTTC 1 cut(s) 197
Lsp1109I GCAGC 3 cut(s) 4, 209, 461
LweI GCATC 1 cut(s) 515
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 362, 446, 503
MalI GATC 2 cut(s) 27, 440
MboI GATC 2 cut(s) 25, 438
MboII GAAGA 8 cut(s) 44, 56, 211, 214, 241, 259, 343, 427
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 209, 216
MluCI AATT 5 cut(s) 257, 341, 425, 514, 574
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 2 cut(s) 110, 539
Mph1103I ATGCAT 1 cut(s) 583
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 4 cut(s) 273, 357, 441, 525
NdeII GATC 2 cut(s) 25, 438
NlaIII CATG 1 cut(s) 585
NlaIV GGNNCC 2 cut(s) 78, 214
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 583
PasI CCCWGGG 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 197
PfeI GAWTC 2 cut(s) 68, 83
PkrI GCNGC 4 cut(s) 19, 128, 224, 476
Psp6I CCWGG 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 78, 214
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 212, 213
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 438
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 377, 461
RsaNI GTAC 2 cut(s) 376, 460
SapI GCTCTTC 1 cut(s) 197
SatI GCNGC 4 cut(s) 18, 127, 223, 475
Sau3AI GATC 2 cut(s) 25, 438
Sau96I GGNCC 2 cut(s) 212, 213
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 2 cut(s) 209, 216
SetI ASST 3 cut(s) 19, 153, 170
SfaNI GCATC 1 cut(s) 515
Sse9I AATT 5 cut(s) 257, 341, 425, 514, 574
SsiI CCGC 8 cut(s) 127, 267, 276, 351, 360, 435, 444, 519
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 76
TasI AATT 5 cut(s) 257, 341, 425, 514, 574
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 68, 83
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 3 cut(s) 17, 222, 474
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 2 cut(s) 266, 350
TspRI CASTG 1 cut(s) 46
XmaJI CCTAGG 1 cut(s) 182
XspI CTAG 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.