MD05G1350000.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009756
Physical Location & Seq
Reverse (-)
46779099 .. 46785135
6037 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1350000.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGCCTCCCTAGCCCCTCTCGTATTATTTCTCTTATGTTTTTTAACTTATATTGCTCCAACATTTCAAGCCTATTCCCCAGACTACTACACCACCCCCAATATCACTCTCAAAAACATCATGAACGTGGTAGACTCGTGTTGGCGCACCAAATCCAATTGGGCCACCAACCGCCGAGCCTTGGCCGACTGCGCAGTAGGCTATGGCAAAGACGCATTGGGAGGAAAATACGGGCGTACTTATGTAGTCACAACCTCAAATGATGACTCAGTAAACCCTAAACCCGGCTCACTCCGTTACGGGGTGATCCAAACGCAGCCATTATGGATCGTTTTTGCCAAGGACATGGTGATCACGCTAAAAAATGAGCTCATTATGAATAGTTTCAAGACCATAGATGGTAGAGGTGCTAAAGTTGAAATTGCGTACGGACCATGCATAACGGTGCAGGGCGTTAGCCACGTTATTATACATGGAATAAGCATTCATGATTGTAAGCCTGGGAAGGGTGGGAATGTTAGGAGCACTCCTACACATATTGGGAAGCGTCGAGGGTGCGACGGAGATGCTATTGCTGTCTTTGCTTCTTCGCATGTTTGGATAGACCATTGCTTCCTAGCTCGTAGCGCAGATGGCCTCCTTGATGTCACCCATGCTTCCACTGCCGTCACCATCACAAACAACTATTTCTCTCAGCATGACAAAACAAGCTTTATCCAAGGAGATCCAGCTCTTGAAGTTGGGAAAGTGGTGCCTCTTCGATTTTTGAACAAGCAATCCTGTCGGGGATCTGGCTCTCGAGATTCGGAGAACGATGCCTCTTCGATTTTGAGAAAGCAAATCCTGCTGGGGGTCTGGCTCTTCGAGATTCGGAGAGCGGTGTCTCTTCGATTTTTGAGAAAGTTGGGCATGTTTGCTAGTCTACCTTGCCACGAAGCACAGAGGTTGACACACCGGACTTTCCAATTATCCAGCAGTGGTACTGTTCCTTTACCCTTGTGGCGATGTAGTGGGAGCTGCAAGCTTCAAGTGTCTCAACTTTGTCAGAGAACTTTGGCAAAGTTATCTGTGGTACCCATGAGCTACTGTTGCATGTGGGAAGTGGGTGATTTGAACAGTGCGATTCATGTGCTTTCTTCTACTTTCTCTTCGAAGTTCGTCAGGTTTGGGTATGCGCACCTGGCCAACAATAGATATGACGAGTGGAAAATGTATGCCGTAGGAGGCAGTGCCAATCCAACAATTTTCAGTGAAGGGAACTACTTCATAGCTCCTGAAACTTCTTATGCCAAACAGGTTACAAAGAAAGAGGCTGGAGGCAGTTGGAATAATTGGAAATGGAGATCTTCAGGGGATGTGTTCAAGAATGGTGCTTTTTTTGTTCAATCTGGATATGGAAACTGCTATCCACTTTACTCTCGAAAGCAGTCGTTTAAGGTCTTTCCAGGAGCCATGGTTCCTGCTTTAACCTCAAATGCAGGTCCTCTACGCTGCTTTGTTGGCAAAGTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

505

Amino Acids

55.64

Weight (kDa)

9.44

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 125 - 239 5e-07 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11920 AT1G30350 AT4G22080 AT4G22090
fragaria_vesca FvH4_3g01680
malus_domestica MD00G1202500.v1.1 MD05G1350000.v1.1 MD10G1324800.v1.1
prunus_persica Prupe.4G017400_v2.0.a1 Prupe.4G020600_v2.0.a1
pyrus_communis pycom10g27510
rosa_chinensis RchiOBHm_Chr5g0002531
rosa_laevigata RLG00000031037
rosa_multiflora Rmu_sc0010731.1_g000004
rosa_roxburghii Rroxscaffold_1G00073370
rosa_rugosa Rorug04G0396700
rosa_samantha Rh5AG020100 Rh5BG022800 Rh5CG022100 Rh5DG021200
rosa_wichuraiana Rw5G001850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 193, 1176
Acc36I ACCTGC 1 cut(s) 1469
Acc65I GGTACC 1 cut(s) 1072
AccB1I GGYRCC 2 cut(s) 751, 1072
AccBSI CCGCTC 1 cut(s) 878
AccI GTMKAC 2 cut(s) 132, 922
AciI CCGC 2 cut(s) 172, 878
AclWI GGATC 4 cut(s) 301, 335, 719, 796
AcoI YGGCCR 2 cut(s) 183, 1182
AcuI CTGAAG 1 cut(s) 1332
AfaI GTAC 4 cut(s) 238, 428, 982, 1074
AfiI CCNNNNNNNGG 5 cut(s) 181, 284, 301, 506, 850
AgsI TTSAA 9 cut(s) 68, 388, 419, 737, 769, 1028, 1114, 1363, 1385
AjnI CCWGG 3 cut(s) 499, 1179, 1444
AloI GAACNNNNNNTCC 4 cut(s) 761, 793, 1440, 1472
AluBI AGCT 8 cut(s) 370, 620, 711, 731, 1017, 1024, 1083, 1271
AluI AGCT 8 cut(s) 370, 620, 711, 731, 1017, 1024, 1083, 1271
Alw21I GWGCWC 2 cut(s) 372, 527
Alw26I GTCTC 2 cut(s) 888, 1038
AlwI GGATC 4 cut(s) 301, 335, 719, 796
Ama87I CYCGRG 1 cut(s) 798
AoxI GGCC 5 cut(s) 3, 162, 183, 634, 1182
ApeKI GCWGC 3 cut(s) 316, 1017, 1491
Asp700I GAANNNNTTC 2 cut(s) 383, 1262
Asp718I GGTACC 1 cut(s) 1072
AspLEI GCGC 4 cut(s) 147, 194, 629, 1177
AspS9I GGNCC 3 cut(s) 162, 431, 1481
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 5 cut(s) 316, 361, 640, 661, 1118
AsuII TTCGAA 1 cut(s) 1151
AvaI CYCGRG 1 cut(s) 798
AvaII GGWCC 2 cut(s) 431, 1481
BalI TGGCCA 1 cut(s) 1184
BanI GGYRCC 2 cut(s) 751, 1072
BanII GRGCYC 1 cut(s) 372
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 2 cut(s) 372, 527
BbvI GCAGC 3 cut(s) 328, 1004, 1478
BccI CCATC 3 cut(s) 392, 626, 680
BceAI ACGGC 2 cut(s) 650, 1202
BcgI CGANNNNNNTGC 6 cut(s) 548, 582, 764, 798, 1111, 1145
BciT130I CCWGG 3 cut(s) 501, 1181, 1446
BclI TGATCA 1 cut(s) 351
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 888, 1038
BfaI CTAG 3 cut(s) 11, 617, 918
BfuAI ACCTGC 1 cut(s) 1469
BglII AGATCT 1 cut(s) 1343
BisI GCNGC 3 cut(s) 317, 1018, 1492
BlsI GCNGC 3 cut(s) 318, 1019, 1493
Bme1390I CCNGG 4 cut(s) 285, 501, 1181, 1446
Bme18I GGWCC 2 cut(s) 431, 1481
BmeT110I CYCGRG 1 cut(s) 798
BmgT120I GGNCC 3 cut(s) 162, 431, 1481
BmiI GGNNCC 4 cut(s) 753, 1074, 1450, 1458
BmrFI CCNGG 4 cut(s) 285, 501, 1181, 1446
BmsI GCATC 2 cut(s) 556, 805
BpmI CTGGAG 1 cut(s) 1335
Bpu14I TTCGAA 1 cut(s) 1151
BpuMI CCSGG 1 cut(s) 285
BsaJI CCNNGG 5 cut(s) 180, 339, 500, 718, 1452
BsaWI WCCGGW 1 cut(s) 954
BsaXI ACNNNNNCTCC 2 cut(s) 1440, 1470
Bsc4I CCNNNNNNNGG 5 cut(s) 181, 284, 301, 506, 850
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 3 cut(s) 501, 1181, 1446
BseDI CCNNGG 5 cut(s) 180, 339, 500, 718, 1452
BseGI GGATG 1 cut(s) 1360
BseLI CCNNNNNNNGG 5 cut(s) 181, 284, 301, 506, 850
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 2 cut(s) 282, 707
BseXI GCAGC 3 cut(s) 328, 1004, 1478
BseYI CCCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 467
BshFI GGCC 5 cut(s) 5, 164, 185, 636, 1184
BshNI GGYRCC 2 cut(s) 751, 1072
BsiHKAI GWGCWC 2 cut(s) 372, 527
BsiHKCI CYCGRG 1 cut(s) 798
BsiSI CCGG 2 cut(s) 285, 955
BsiWI CGTACG 1 cut(s) 426
BslI CCNNNNNNNGG 5 cut(s) 181, 284, 301, 506, 850
BsmAI GTCTC 2 cut(s) 888, 1038
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 5 cut(s) 5, 164, 185, 636, 1184
BsoBI CYCGRG 1 cut(s) 798
Bsp119I TTCGAA 1 cut(s) 1151
Bsp1286I GDGCHC 2 cut(s) 372, 527
Bsp143I GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
Bsp19I CCATGG 1 cut(s) 1452
BspACI CCGC 2 cut(s) 172, 878
BspANI GGCC 5 cut(s) 5, 164, 185, 636, 1184
BspCNI CTCAG 2 cut(s) 281, 706
BspHI TCATGA 2 cut(s) 120, 487
BspLI GGNNCC 4 cut(s) 753, 1074, 1450, 1458
BspMI ACCTGC 1 cut(s) 1469
BspPI GGATC 4 cut(s) 301, 335, 719, 796
BspQI GCTCTTC 1 cut(s) 866
BspT104I TTCGAA 1 cut(s) 1151
BspT107I GGYRCC 2 cut(s) 751, 1072
BsrBI CCGCTC 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 607
BssECI CCNNGG 5 cut(s) 180, 339, 500, 718, 1452
BssMI GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
BssSI CACGAG 1 cut(s) 136
BssT1I CCWWGG 4 cut(s) 180, 339, 718, 1452
Bst2BI CACGAG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 501, 1181, 1446
Bst4CI ACNGT 4 cut(s) 445, 985, 1088, 1118
Bst6I CTCTTC 5 cut(s) 762, 826, 866, 891, 1153
BstAPI GCANNNNNTGC 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 1151
BstC8I GCNNGC 1 cut(s) 1022
BstDEI CTNAG 2 cut(s) 268, 693
BstDSI CCRYGG 1 cut(s) 1452
BstF5I GGATG 1 cut(s) 1360
BstHHI GCGC 4 cut(s) 147, 194, 629, 1177
BstKTI GATC 6 cut(s) 309, 330, 354, 727, 791, 1346
BstMAI GTCTC 2 cut(s) 888, 1038
BstMBI GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
BstNI CCWGG 3 cut(s) 501, 1181, 1446
BstNSI RCATGY 3 cut(s) 596, 913, 1096
BstSCI CCNGG 4 cut(s) 283, 499, 1179, 1444
BstV1I GCAGC 3 cut(s) 328, 1004, 1478
BstX2I RGATCY 3 cut(s) 724, 788, 1343
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 3 cut(s) 724, 788, 1343
BsuRI GGCC 5 cut(s) 5, 164, 185, 636, 1184
BtgI CCRYGG 1 cut(s) 1452
BtgZI GCGATG 1 cut(s) 1018
BtsCI GGATG 1 cut(s) 1360
BtsI GCAGTG 3 cut(s) 660, 982, 1234
BtsIMutI CAGTG 5 cut(s) 660, 982, 1123, 1234, 1255
BveI ACCTGC 1 cut(s) 1469
Cac8I GCNNGC 1 cut(s) 1022
CciI TCATGA 2 cut(s) 120, 487
CfoI GCGC 4 cut(s) 147, 194, 629, 1177
Cfr13I GGNCC 3 cut(s) 162, 431, 1481
CseI GACGC 2 cut(s) 221, 536
Csp6I GTAC 4 cut(s) 237, 427, 981, 1073
CviQI GTAC 4 cut(s) 237, 427, 981, 1073
DdeI CTNAG 2 cut(s) 268, 693
DpnI GATC 6 cut(s) 308, 329, 353, 726, 790, 1345
DpnII GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
EaeI YGGCCR 2 cut(s) 183, 1182
Eam1104I CTCTTC 5 cut(s) 762, 826, 866, 891, 1153
EarI CTCTTC 5 cut(s) 762, 826, 866, 891, 1153
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 4 cut(s) 180, 339, 718, 1452
Eco24I GRGCYC 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 431, 1481
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 1332
Eco88I CYCGRG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 1481
EcoRII CCWGG 3 cut(s) 499, 1179, 1444
EcoT14I CCWWGG 4 cut(s) 180, 339, 718, 1452
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 4 cut(s) 180, 339, 718, 1452
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 2 cut(s) 132, 922
Fnu4HI GCNGC 3 cut(s) 317, 1018, 1492
FokI GGATG 1 cut(s) 1367
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 317, 1018, 1492
FspAI RTGCGCAY 1 cut(s) 1176
FspBI CTAG 3 cut(s) 11, 617, 918
FspI TGCGCA 2 cut(s) 193, 1176
GlaI GCGC 4 cut(s) 146, 193, 628, 1176
GluI GCNGC 3 cut(s) 317, 1018, 1492
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 1 cut(s) 1335
HaeIII GGCC 5 cut(s) 5, 164, 185, 636, 1184
HapII CCGG 2 cut(s) 285, 955
HgaI GACGC 2 cut(s) 221, 536
HhaI GCGC 4 cut(s) 147, 194, 629, 1177
Hin6I GCGC 4 cut(s) 145, 192, 627, 1175
HinP1I GCGC 4 cut(s) 145, 192, 627, 1175
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HindIII AAGCTT 2 cut(s) 709, 1022
HinfI GANTC 5 cut(s) 134, 266, 803, 868, 1123
HpaII CCGG 2 cut(s) 285, 955
HphI GGTGA 5 cut(s) 316, 361, 640, 661, 1118
Hpy166II GTNNAC 4 cut(s) 133, 274, 923, 948
Hpy188I TCNGA 3 cut(s) 808, 873, 1047
Hpy8I GTNNAC 4 cut(s) 133, 274, 923, 948
Hpy99I CGWCG 2 cut(s) 552, 563
HpyAV CCTTC 2 cut(s) 499, 1247
HpyCH4III ACNGT 4 cut(s) 445, 985, 1088, 1118
HpyCH4IV ACGT 2 cut(s) 126, 462
HpyCH4V TGCA 5 cut(s) 438, 448, 1020, 1092, 1478
HpyF3I CTNAG 2 cut(s) 268, 693
HpySE526I ACGT 2 cut(s) 126, 462
HspAI GCGC 4 cut(s) 145, 192, 627, 1175
KpnI GGTACC 1 cut(s) 1076
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
LguI GCTCTTC 1 cut(s) 866
LmnI GCTCC 5 cut(s) 61, 522, 1014, 1276, 1448
Lsp1109I GCAGC 3 cut(s) 328, 1004, 1478
LweI GCATC 2 cut(s) 556, 805
MaeI CTAG 3 cut(s) 11, 617, 918
MaeII ACGT 2 cut(s) 126, 462
MaeIII GTNAC 5 cut(s) 247, 296, 646, 667, 1297
MalI GATC 6 cut(s) 308, 329, 353, 726, 790, 1345
MbiI CCGCTC 1 cut(s) 878
MboI GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
MboII GAAGA 8 cut(s) 579, 749, 813, 853, 878, 1128, 1140, 1338
MfeI CAATTG 1 cut(s) 157
MflI RGATCY 3 cut(s) 724, 788, 1343
MhlI GDGCHC 2 cut(s) 372, 527
MlsI TGGCCA 1 cut(s) 1184
MluCI AATT 5 cut(s) 157, 420, 965, 1242, 1330
MluNI TGGCCA 1 cut(s) 1184
MlyI GAGTC 2 cut(s) 128, 260
MmeI TCCRAC 3 cut(s) 83, 1262, 1304
Mox20I TGGCCA 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 440
MroXI GAANNNNTTC 2 cut(s) 383, 1262
MscI TGGCCA 1 cut(s) 1184
MseI TTAA 4 cut(s) 44, 1434, 1466, 1513
MslI CAYNNNNRTG 2 cut(s) 125, 443
Msp20I TGGCCA 1 cut(s) 1184
MspI CCGG 2 cut(s) 285, 955
MspR9I CCNGG 4 cut(s) 285, 501, 1181, 1446
MunI CAATTG 1 cut(s) 157
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 3 cut(s) 501, 1181, 1446
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 1 cut(s) 1452
NdeII GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
NlaIV GGNNCC 4 cut(s) 753, 1074, 1450, 1458
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 3 cut(s) 247, 646, 667
NsbI TGCGCA 2 cut(s) 193, 1176
NsiI ATGCAT 1 cut(s) 440
NspI RCATGY 3 cut(s) 596, 913, 1096
NspV TTCGAA 1 cut(s) 1151
PaeR7I CTCGAG 1 cut(s) 798
PagI TCATGA 2 cut(s) 120, 487
PciSI GCTCTTC 1 cut(s) 866
PctI GAATGC 1 cut(s) 483
PdmI GAANNNNTTC 2 cut(s) 383, 1262
PfeI GAWTC 3 cut(s) 803, 868, 1123
Pfl23II CGTACG 1 cut(s) 426
PfoI TCCNGGA 1 cut(s) 1444
PkrI GCNGC 3 cut(s) 318, 1019, 1493
PleI GAGTC 2 cut(s) 128, 260
PpsI GAGTC 2 cut(s) 128, 260
PpuMI RGGWCCY 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 1481
Psp6I CCWGG 3 cut(s) 499, 1179, 1444
PspFI CCCAGC 1 cut(s) 847
PspGI CCWGG 3 cut(s) 499, 1179, 1444
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 4 cut(s) 753, 1074, 1450, 1458
PspPI GGNCC 3 cut(s) 162, 431, 1481
PspPPI RGGWCCY 1 cut(s) 1481
PsuI RGATCY 3 cut(s) 724, 788, 1343
RsaI GTAC 4 cut(s) 238, 428, 982, 1074
RsaNI GTAC 4 cut(s) 237, 427, 981, 1073
RseI CAYNNNNRTG 2 cut(s) 125, 443
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 866
SaqAI TTAA 4 cut(s) 44, 1434, 1466, 1513
SatI GCNGC 3 cut(s) 317, 1018, 1492
Sau3AI GATC 6 cut(s) 306, 327, 351, 724, 788, 1343
Sau96I GGNCC 3 cut(s) 162, 431, 1481
SchI GAGTC 2 cut(s) 128, 260
ScrFI CCNGG 4 cut(s) 285, 501, 1181, 1446
SduI GDGCHC 2 cut(s) 372, 527
SfaNI GCATC 2 cut(s) 556, 805
Sfr274I CTCGAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 1151
SinI GGWCC 2 cut(s) 431, 1481
SlaI CTCGAG 1 cut(s) 798
SmiMI CAYNNNNRTG 2 cut(s) 125, 443
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
Sse9I AATT 5 cut(s) 157, 420, 965, 1242, 1330
SsiI CCGC 2 cut(s) 172, 878
SspMI CTAG 3 cut(s) 11, 617, 918
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 4 cut(s) 283, 499, 1179, 1444
StyI CCWWGG 4 cut(s) 180, 339, 718, 1452
TaaI ACNGT 4 cut(s) 445, 985, 1088, 1118
TaiI ACGT 2 cut(s) 129, 465
TaqI TCGA 8 cut(s) 550, 760, 799, 824, 864, 889, 1151, 1420
TasI AATT 5 cut(s) 157, 420, 965, 1242, 1330
TfiI GAWTC 3 cut(s) 803, 868, 1123
Tru1I TTAA 4 cut(s) 44, 1434, 1466, 1513
Tru9I TTAA 4 cut(s) 44, 1434, 1466, 1513
TscAI CASTG 5 cut(s) 667, 982, 1123, 1234, 1255
TseFI GTSAC 3 cut(s) 247, 646, 667
TseI GCWGC 3 cut(s) 316, 1017, 1491
Tsp45I GTSAC 3 cut(s) 247, 646, 667
TspDTI ATGAA 5 cut(s) 137, 392, 476, 1115, 1255
TspGWI ACGGA 3 cut(s) 284, 444, 576
TspRI CASTG 5 cut(s) 667, 982, 1123, 1234, 1255
VpaK11BI GGWCC 2 cut(s) 431, 1481
XceI RCATGY 3 cut(s) 596, 913, 1096
XhoI CTCGAG 1 cut(s) 798
XmiI GTMKAC 2 cut(s) 132, 922
XmnI GAANNNNTTC 2 cut(s) 383, 1262
XspI CTAG 3 cut(s) 11, 617, 918
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.