AT1G32250

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011857
Physical Location & Seq
Forward (+)
11639741 .. 11640393
653 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32250.1

Sequence Viewer

Length: 501 bp
ATGAGTCACAAAGTCTCCAAAAAGTTAGACGAGGAGCAAATCAATGAGCTCCGGGAGATTTTCCGGTCATTTGATCGGAACAAAGACGGAAGCTTAACTCAGCTCGAGCTCGGATCACTTCTCCGAGCACTAGGGGTTAAACCAAGTCCAGACCAATTCGAAACGCTAATAGACAAAGCCGACACTAAAAGCAACGGACTCGTAGAGTTCCCGGAGTTCGTAGCTTTGGTCTCTCCGGAGCTTCTTTCGCCGGCGAAGAGGACAACTCCTTACACGGAGGAGCAGCTTCTCCGATTGTTTAGGATATTCGACACGGATGGCAACGGGTTTATCACTGCGGCGGAATTGGCTCACTCTATGGCTAAGCTTGGCCACGCCTTGACGGTGGCGGAATTGACGGGGATGATAAAGGAGGCGGATAGCGATGGCGATGGCCGGATTAACTTCCAGGAGTTCGCAAAGGCTATTAACTCTGCTGCATTTGATGATATATGGGGTTGA

Protein Analysis

166

Amino Acids

18.42

Weight (kDa)

4.84

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 15 - 77 4.9e-16 EF-hand domain pair
EF-hand_1 PF00036 16 - 44 7.7e-06 EF hand domain
EF-hand_6 PF13405 16 - 45 8.7e-08 EF-hand domain
EF-hand_7 PF13499 93 - 156 5.5e-16 EF-hand domain pair
EF-hand_6 PF13405 96 - 124 5.7e-07 EF-hand domain
EF-hand_1 PF00036 97 - 123 2.3e-06 EF hand domain
EF-hand_8 PF13833 109 - 153 7.1e-09 EF-hand domain pair
EF-hand_1 PF00036 133 - 157 6.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 4 cut(s) 338, 341, 389, 416
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 2 cut(s) 370, 433
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 8 cut(s) 49, 93, 103, 109, 224, 241, 286, 367
AluI AGCT 8 cut(s) 49, 93, 103, 109, 224, 241, 286, 367
Alw21I GWGCWC 3 cut(s) 51, 111, 130
Alw26I GTCTC 2 cut(s) 19, 235
AlwI GGATC 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 104
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 370, 433
ApeKI GCWGC 2 cut(s) 283, 476
AsuC2I CCSGG 2 cut(s) 53, 212
AsuII TTCGAA 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 104
BalI TGGCCA 1 cut(s) 372
BanII GRGCYC 2 cut(s) 51, 111
Bbv12I GWGCWC 3 cut(s) 51, 111, 130
BbvI GCAGC 2 cut(s) 295, 463
BccI CCATC 3 cut(s) 311, 419, 425
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 2 cut(s) 53, 212
BcoDI GTCTC 2 cut(s) 19, 235
BfaI CTAG 1 cut(s) 131
BisI GCNGC 3 cut(s) 284, 339, 477
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 3 cut(s) 285, 340, 478
Bme1390I CCNGG 3 cut(s) 53, 212, 449
BmeT110I CYCGRG 1 cut(s) 104
BmrFI CCNGG 3 cut(s) 53, 212, 449
BplI GAGNNNNNCTC 2 cut(s) 250, 282
Bpu1102I GCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 159
BpuMI CCSGG 2 cut(s) 53, 212
BsaI GGTCTC 1 cut(s) 235
BsaWI WCCGGW 2 cut(s) 63, 235
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 250
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 1 cut(s) 449
BseGI GGATG 2 cut(s) 322, 408
BseMII CTCAG 1 cut(s) 113
BseRI GAGGAG 2 cut(s) 47, 293
BseXI GCAGC 2 cut(s) 295, 463
BshFI GGCC 2 cut(s) 372, 435
BsiHKAI GWGCWC 3 cut(s) 51, 111, 130
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 6 cut(s) 52, 64, 212, 236, 251, 436
BsmAI GTCTC 2 cut(s) 19, 235
BsnI GGCC 2 cut(s) 372, 435
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 3 cut(s) 51, 111, 130
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 2 cut(s) 73, 113
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 4 cut(s) 338, 341, 389, 416
BspANI GGCC 2 cut(s) 372, 435
BspCNI CTCAG 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 235
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 250
BssMI GATC 2 cut(s) 73, 113
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 99, 363
BstF5I GGATG 2 cut(s) 322, 408
BstKTI GATC 2 cut(s) 76, 116
BstMAI GTCTC 2 cut(s) 19, 235
BstMBI GATC 2 cut(s) 73, 113
BstMWI GCNNNNNNNGC 2 cut(s) 247, 347
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 3 cut(s) 51, 210, 447
BstV1I GCAGC 2 cut(s) 295, 463
BsuRI GGCC 2 cut(s) 372, 435
BtgZI GCGATG 2 cut(s) 438, 444
BtsCI GGATG 2 cut(s) 322, 408
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 250
DdeI CTNAG 2 cut(s) 99, 363
DpnI GATC 2 cut(s) 75, 115
DpnII GATC 2 cut(s) 73, 113
EaeI YGGCCR 2 cut(s) 370, 433
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 3 cut(s) 356, 404, 431
Ecl136II GAGCTC 2 cut(s) 49, 109
Eco24I GRGCYC 2 cut(s) 51, 111
Eco31I GGTCTC 1 cut(s) 235
Eco53kI GAGCTC 2 cut(s) 49, 109
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 2 cut(s) 49, 109
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 2 cut(s) 51, 111
FaiI YATR 3 cut(s) 359, 491, 493
Fnu4HI GCNGC 3 cut(s) 284, 339, 477
FokI GGATG 2 cut(s) 329, 415
FriOI GRGCYC 2 cut(s) 51, 111
Fsp4HI GCNGC 3 cut(s) 284, 339, 477
FspBI CTAG 1 cut(s) 131
GluI GCNGC 3 cut(s) 284, 339, 477
HaeIII GGCC 2 cut(s) 372, 435
HapII CCGG 6 cut(s) 52, 64, 212, 236, 251, 436
HindIII AAGCTT 2 cut(s) 91, 365
HinfI GANTC 2 cut(s) 4, 198
HpaII CCGG 6 cut(s) 52, 64, 212, 236, 251, 436
Hpy188I TCNGA 4 cut(s) 78, 113, 125, 293
Hpy188III TCNNGA 2 cut(s) 149, 236
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4V TGCA 1 cut(s) 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 247, 347
HpyF3I CTNAG 2 cut(s) 99, 363
Kpn2I TCCGGA 1 cut(s) 235
KroI GCCGGC 1 cut(s) 250
KroNI GCCGGC 1 cut(s) 252
Kzo9I GATC 2 cut(s) 73, 113
LmnI GCTCC 4 cut(s) 34, 54, 238, 280
LpnPI CCDG 9 cut(s) 65, 77, 162, 225, 249, 264, 434, 449, 461
Lsp1109I GCAGC 2 cut(s) 295, 463
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 5
MalI GATC 2 cut(s) 75, 115
MboI GATC 2 cut(s) 73, 113
MboII GAAGA 1 cut(s) 268
MhlI GDGCHC 3 cut(s) 51, 111, 130
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 3 cut(s) 155, 344, 392
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 2 cut(s) 13, 192
MnlI CCTC 4 cut(s) 25, 252, 271, 406
Mox20I TGGCCA 1 cut(s) 372
MreI CGCCGGCG 1 cut(s) 250
MroI TCCGGA 1 cut(s) 235
MroNI GCCGGC 1 cut(s) 250
MscI TGGCCA 1 cut(s) 372
MseI TTAA 4 cut(s) 95, 138, 441, 468
Msp20I TGGCCA 1 cut(s) 372
MspI CCGG 6 cut(s) 52, 64, 212, 236, 251, 436
MspR9I CCNGG 3 cut(s) 53, 212, 449
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 247, 347
NaeI GCCGGC 1 cut(s) 252
NciI CCSGG 2 cut(s) 53, 212
NdeII GATC 2 cut(s) 73, 113
NgoMIV GCCGGC 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 5
NspV TTCGAA 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 104
PdiI GCCGGC 1 cut(s) 252
PfoI TCCNGGA 3 cut(s) 51, 210, 447
PkrI GCNGC 3 cut(s) 285, 340, 478
PleI GAGTC 2 cut(s) 12, 192
PpsI GAGTC 2 cut(s) 12, 192
Psp124BI GAGCTC 2 cut(s) 51, 111
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspXI VCTCGAGB 1 cut(s) 104
SacI GAGCTC 2 cut(s) 51, 111
SaqAI TTAA 4 cut(s) 95, 138, 441, 468
SatI GCNGC 3 cut(s) 284, 339, 477
Sau3AI GATC 2 cut(s) 73, 113
SchI GAGTC 2 cut(s) 13, 192
ScrFI CCNGG 3 cut(s) 53, 212, 449
SduI GDGCHC 3 cut(s) 51, 111, 130
SetI ASST 8 cut(s) 51, 95, 105, 111, 226, 243, 288, 369
Sfr274I CTCGAG 1 cut(s) 104
SfuI TTCGAA 1 cut(s) 159
SgrAI CRCCGGYG 1 cut(s) 250
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 155, 344, 392
SsiI CCGC 4 cut(s) 338, 341, 389, 416
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 2 cut(s) 51, 111
StyD4I CCNGG 3 cut(s) 51, 210, 447
TaaI ACNGT 1 cut(s) 385
TaqI TCGA 3 cut(s) 105, 159, 309
TasI AATT 3 cut(s) 155, 344, 392
TauI GCSGC 1 cut(s) 341
Tru1I TTAA 4 cut(s) 95, 138, 441, 468
Tru9I TTAA 4 cut(s) 95, 138, 441, 468
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 2 cut(s) 283, 476
Tsp45I GTSAC 1 cut(s) 5
TspGWI ACGGA 4 cut(s) 102, 210, 290, 329
TspRI CASTG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.