Rorug07G0126400

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0011205
Physical Location & Seq
Forward (+)
9968436 .. 9971734
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0126400.1

Sequence Viewer

Length: 2127 bp
ATGGTGCACAGCTTCCACCCCTACACGGCGGCCTCGGCCCGCTTCCGGCAAATCTCGGTGCCCTTCAACCTGCGCAAGACAAAGCCATGGTGGTCGGAGATCTTGGACCCGGGATCCGAAACAGTGACACGGTGGAACTACATCTTCTTCGTGATCTGCATCATGGGCCTCTTCCTCGACCCCCTTTTCTTCTTCTTACCCCTCATCGGCGGAAAAGCCTGCATGGAGACCGACCTCCAAATCAGCATCTATGTCACCTCCATCCGCACAATCGCCGACTTGTTCTACATCTTCCACATTATCATCAAGTTTCGTATTGCCTTTATTGCCCCTGGCTCTAGGGTTTTCGGCCGCGGCCAGCTCGTTAAGGACCCCTGGGCCATTGCCTCGCGCTACCTCAAAAAGGACTTCTCCATTGACCTAGCCGCCTGTCTACCACTCCCCCAGATTATGATTTGGTTTGTGATTCCAGCGACGAGAAACTCAACAGCTGCTTATACTAATCATACACTTTCTTTGATCGTTCTCATTCAATACATCCCCCGATTTATTCAGCTTTTCCCTCTAAACCGGCGGATTGCTAAGAACACTGGTGTTGTAGCCAAGACTGCTTGGTCAGGGGCAGTGTACAATCTTATTCTTTTTGCGCTAACTGCACATATTGTTGGATCTTCGTGGTATGTGTTGTCCATTAAACGGCAGTACGAGTGTTGGAAACAGGAATGTGGCAAGGAACATAATGGGACACATTCTCCATCCTGTAATCTTGAGTTTCTTGATTGTAAAACTACAAATAATCGTGAACGCCAAGCTTGGTTGGAGAAAACAAACGTGAGTGAGAACTGCGATGCTATAAATGATGAGGGAAAATTTCAGTTTGGAATGTTTAAAGAGGCATTCAAAAATGACGTCGCTTCTGCACCCTTCATTAGTAAATATTTCTATTGCCTTTGGTGGGGTATGAGAAATTTATGTGCATATGGACAAGATATTGAAGTAAGCACTATGGTTGGTGAAACCTCGTTTTGCATTACCATTGCGTCAATTGGCCTCGTCCAGTTTTCACATCTCATGAGTCAGATACAGACCTTTCTGCAATCTACGACGGCTAGACTTGAAGAGTGGAGGGTCAAAAGAAGAGACACAGAGGAGTGGATGAGGCACCGTCAACTCCCTCCAGAATTGCAAGAACGTGTGCGTCGGTTTGTTCAATATCAGTGGATTGCCACAAGAGGTGTTGATGAAAAATCCATCTTAAATTCCCTGCCTTTGGATATACGACGTCAAATTCAAAGGCATCTATGCCTTGCCCATGTTCGCCGAGTTCCCTTCTTCGCACAAATGGATGATCAGCTCTTGGACGCCATATGCGAACGCCTCAAGTCATCCTTAAATACCAGAGACACACACATCTTTCGGGAGGGTGCTCCGGTGAGTGAGATGCTTTTCATTGTCAGAGGGCAACTCGAAAGCTCCACGACTGATGGTGGAAGGACAGGATTCTTCAACTCCATCACCCTAAGAGCCGGTGACTTCTGTGGTGAAGAGTTGCTGACATGGGCCTTAATGCCTTCATCCAGTCTAAACCTTCCGGTCTCAACTCGAACTGTGAAGTCCCTCACTGAAGTCGAGGCATTTGCACTCCGAGCTGAAGACCTCAAGTATGTTGCGAGTCAATTTAAGCGCCTTCACAGCAAAAAACTGCAGCACGCATTTAGGTACTACTCCCACCAGTGGAGGACTTGGGGAGCTTGTTATATACAAGTTGCGTGGAGACGATACAAAAAGAGGAAATTGGCGCTGGATTTGTTGAGAGAGGAGGAGTACTATTACACACATGTTTTGGACCAAGACCCTGACTATAACAATGGATCAACTAGTGGTGCAGACGACGACGATGACGACGACAACGACGATGAAGGTCCAAGTAAGACCTCATCGCTGCGTCGTATGGCAGAGCAATTGCACATTCCACTCGGGCCTGCAATTTTGGCTTCGAAGTTTGCTGCAACCACCAAAAGAGGACTTCAGAAGGTGGCAACACGGCTTGACCGTGAAACTAGCAGTGCCAAAATGCCCAAAAAGTTGTTTAAGCCGAACGAGCCAGATTTCTCAAAAGATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

708

Amino Acids

81.73

Weight (kDa)

9.04

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 45 - 371 7.1e-12 Ion transport protein
cNMP_binding PF00027 468 - 556 4.9e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35490
fragaria_vesca FvH4_5g22910 FvH4_5g22910 FvH4_5g22910
malus_domestica MD06G1025700.v1.1 MD16G1280700.v1.1
prunus_persica Prupe.5G029300_v2.0.a1
pyrus_communis pycom06g02090 pycom16g25080
rosa_chinensis RchiOBHm_Chr7g0211641
rosa_laevigata RLG00000002988
rosa_multiflora Rmu_sc0003760.1_g000015 Rmu_sc0004232.1_g000010
rosa_roxburghii Rroxscaffold_3G00247650
rosa_rugosa Rorug07G0126400
rosa_samantha Rh7AG259200 Rh7BG253500 Rh7CG276200 Rh7DG266800
rosa_wichuraiana Rw7G022160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 613
AatII GACGTC 2 cut(s) 912, 1285
Acc16I TGCGCA 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 58, 1161
AccI GTMKAC 1 cut(s) 433
AccII CGCG 2 cut(s) 354, 391
AciI CCGC 8 cut(s) 29, 40, 210, 265, 352, 354, 426, 574
AclWI GGATC 4 cut(s) 108, 121, 676, 1879
AcoI YGGCCR 2 cut(s) 349, 355
AcsI RAATTY 4 cut(s) 869, 967, 1258, 1287
AcuI CTGAAG 3 cut(s) 1644, 1671, 2012
AcyI GRCGYC 3 cut(s) 909, 1282, 1362
AfaI GTAC 4 cut(s) 629, 704, 1721, 1826
AfiI CCNNNNNNNGG 8 cut(s) 25, 45, 206, 403, 696, 955, 1270, 1734
AflIII ACRYGT 2 cut(s) 1192, 1837
AgsI TTSAA 8 cut(s) 67, 533, 901, 995, 1118, 1211, 1292, 1507
AhlI ACTAGT 1 cut(s) 1877
AjnI CCWGG 2 cut(s) 331, 374
AluBI AGCT 9 cut(s) 12, 361, 491, 556, 812, 1354, 1473, 1649, 1751
AluI AGCT 9 cut(s) 12, 361, 491, 556, 812, 1354, 1473, 1649, 1751
Alw21I GWGCWC 2 cut(s) 9, 1429
Alw26I GTCTC 5 cut(s) 221, 1134, 1395, 1600, 1768
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 4 cut(s) 108, 121, 676, 1879
Ama87I CYCGRG 2 cut(s) 109, 1976
AoxI GGCC 9 cut(s) 30, 36, 166, 349, 355, 378, 1048, 1560, 1979
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 4 cut(s) 491, 1705, 1942, 2006
ApoI RAATTY 4 cut(s) 869, 967, 1258, 1287
ArsI GACNNNNNNTTYG 2 cut(s) 894, 926
AspLEI GCGC 5 cut(s) 75, 393, 649, 1686, 1801
AspS9I GGNCC 9 cut(s) 37, 106, 166, 370, 378, 1560, 1846, 1922, 1979
AsuC2I CCSGG 2 cut(s) 110, 111
AsuHPI GGTGA 6 cut(s) 247, 1025, 1444, 1507, 1541, 1553
AsuII TTCGAA 1 cut(s) 1997
AvaI CYCGRG 2 cut(s) 109, 1976
AvaII GGWCC 4 cut(s) 106, 370, 1846, 1922
BaeGI GKGCMC 2 cut(s) 9, 63
BamHI GGATCC 1 cut(s) 113
BanI GGYRCC 2 cut(s) 58, 1161
BbsI GAAGAC 1 cut(s) 1659
Bbv12I GWGCWC 2 cut(s) 9, 1429
BbvI GCAGC 4 cut(s) 478, 1717, 1929, 1993
BccI CCATC 5 cut(s) 269, 763, 1259, 1478, 1520
BceAI ACGGC 4 cut(s) 42, 713, 1122, 2060
BcgI CGANNNNNNTGC 2 cut(s) 1619, 1653
BciT130I CCWGG 2 cut(s) 333, 376
BclI TGATCA 1 cut(s) 1348
BcnI CCSGG 2 cut(s) 110, 111
BcoDI GTCTC 5 cut(s) 221, 1134, 1395, 1600, 1768
BcuI ACTAGT 1 cut(s) 1877
BfaI CTAG 5 cut(s) 339, 422, 1110, 1878, 2061
BfmI CTRYAG 1 cut(s) 1703
BfoI RGCGCY 2 cut(s) 1687, 1802
BfuAI ACCTGC 1 cut(s) 78
BglII AGATCT 1 cut(s) 99
BisI GCNGC 8 cut(s) 30, 352, 355, 426, 492, 1706, 1943, 2007
BlsI GCNGC 8 cut(s) 31, 353, 356, 427, 493, 1707, 1944, 2008
BmcAI AGTACT 1 cut(s) 1826
Bme1390I CCNGG 4 cut(s) 110, 111, 333, 376
Bme18I GGWCC 4 cut(s) 106, 370, 1846, 1922
BmeT110I CYCGRG 2 cut(s) 109, 1976
BmgT120I GGNCC 9 cut(s) 37, 106, 166, 370, 378, 1560, 1846, 1922, 1979
BmiI GGNNCC 5 cut(s) 60, 108, 115, 372, 1163
BmrFI CCNGG 4 cut(s) 110, 111, 333, 376
BmsI GCATC 5 cut(s) 168, 255, 838, 1306, 1431
BpiI GAAGAC 1 cut(s) 1659
BplI GAGNNNNNCTC 2 cut(s) 1449, 1481
BpmI CTGGAG 1 cut(s) 1161
Bpu14I TTCGAA 1 cut(s) 1997
BpuEI CTTGAG 3 cut(s) 788, 1364, 1643
BpuMI CCSGG 2 cut(s) 110, 111
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 3 cut(s) 909, 1282, 1362
BsaI GGTCTC 2 cut(s) 221, 1600
BsaJI CCNNGG 7 cut(s) 33, 86, 109, 331, 352, 374, 375
BsaWI WCCGGW 2 cut(s) 1429, 1591
BsaXI ACNNNNNCTCC 2 cut(s) 736, 766
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 45, 206, 403, 696, 955, 1270, 1734
Bse118I RCCGGY 2 cut(s) 570, 1526
Bse1I ACTGG 4 cut(s) 595, 1057, 1578, 1732
Bse3DI GCAATG 2 cut(s) 381, 1035
Bse8I GATNNNNATC 1 cut(s) 158
BseBI CCWGG 2 cut(s) 333, 376
BseDI CCNNGG 7 cut(s) 33, 86, 109, 331, 352, 374, 375
BseGI GGATG 7 cut(s) 261, 537, 755, 1161, 1351, 1385, 1574
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 8 cut(s) 25, 45, 206, 403, 696, 955, 1270, 1734
BseMI GCAATG 2 cut(s) 381, 1035
BseNI ACTGG 4 cut(s) 595, 1057, 1578, 1732
BseRI GAGGAG 3 cut(s) 1163, 1832, 1835
BseSI GKGCMC 2 cut(s) 9, 63
BseX3I CGGCCG 1 cut(s) 349
BseXI GCAGC 4 cut(s) 478, 1717, 1929, 1993
BsgI GTGCAG 3 cut(s) 639, 903, 1905
Bsh1236I CGCG 2 cut(s) 354, 391
Bsh1285I CGRYCG 1 cut(s) 352
BshFI GGCC 9 cut(s) 32, 38, 168, 351, 357, 380, 1050, 1562, 1981
BshNI GGYRCC 2 cut(s) 58, 1161
BsiEI CGRYCG 1 cut(s) 352
BsiHKAI GWGCWC 2 cut(s) 9, 1429
BsiHKCI CYCGRG 2 cut(s) 109, 1976
BsiSI CCGG 6 cut(s) 46, 110, 571, 1430, 1527, 1592
BslFI GGGAC 2 cut(s) 757, 1600
BslI CCNNNNNNNGG 8 cut(s) 25, 45, 206, 403, 696, 955, 1270, 1734
BsmAI GTCTC 5 cut(s) 221, 1134, 1395, 1600, 1768
BsmBI CGTCTC 1 cut(s) 1768
BsmFI GGGAC 2 cut(s) 757, 1600
BsmI GAATGC 1 cut(s) 896
BsnI GGCC 9 cut(s) 32, 38, 168, 351, 357, 380, 1050, 1562, 1981
Bso31I GGTCTC 2 cut(s) 221, 1600
BsoBI CYCGRG 2 cut(s) 109, 1976
Bsp119I TTCGAA 1 cut(s) 1997
Bsp1286I GDGCHC 3 cut(s) 9, 63, 1429
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 8 cut(s) 29, 40, 210, 265, 352, 354, 426, 574
BspANI GGCC 9 cut(s) 32, 38, 168, 351, 357, 380, 1050, 1562, 1981
BspFNI CGCG 2 cut(s) 354, 391
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 5 cut(s) 60, 108, 115, 372, 1163
BspMAI CTGCAG 1 cut(s) 1707
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 4 cut(s) 108, 121, 676, 1879
BspT104I TTCGAA 1 cut(s) 1997
BspT107I GGYRCC 2 cut(s) 58, 1161
BspTNI GGTCTC 2 cut(s) 221, 1600
BsrDI GCAATG 2 cut(s) 381, 1035
BsrFI RCCGGY 2 cut(s) 570, 1526
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 4 cut(s) 595, 1057, 1578, 1732
BssAI RCCGGY 2 cut(s) 570, 1526
BssECI CCNNGG 7 cut(s) 33, 86, 109, 331, 352, 374, 375
BssMI GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
BssNI GRCGYC 3 cut(s) 909, 1282, 1362
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 333, 376
Bst4CI ACNGT 5 cut(s) 124, 132, 1166, 1609, 2054
Bst6I CTCTTC 4 cut(s) 176, 1113, 1132, 1539
BstACI GRCGYC 3 cut(s) 909, 1282, 1362
BstAUI TGTACA 1 cut(s) 627
BstBI TTCGAA 1 cut(s) 1997
BstC8I GCNNGC 5 cut(s) 40, 220, 359, 1710, 1983
BstDEI CTNAG 2 cut(s) 582, 1520
BstDSI CCRYGG 2 cut(s) 86, 352
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 7 cut(s) 261, 537, 755, 1161, 1351, 1385, 1574
BstFNI CGCG 2 cut(s) 354, 391
BstH2I RGCGCY 2 cut(s) 1687, 1802
BstHHI GCGC 5 cut(s) 75, 393, 649, 1686, 1801
BstKTI GATC 7 cut(s) 102, 116, 156, 522, 671, 1351, 1874
BstMAI GTCTC 5 cut(s) 221, 1134, 1395, 1600, 1768
BstMBI GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 9 cut(s) 35, 165, 326, 608, 653, 1646, 1692, 1991, 2101
BstNI CCWGG 2 cut(s) 333, 376
BstNSI RCATGY 1 cut(s) 1841
BstSCI CCNGG 4 cut(s) 108, 109, 331, 374
BstSFI CTRYAG 1 cut(s) 1703
BstSLI GKGCMC 2 cut(s) 9, 63
BstUI CGCG 2 cut(s) 354, 391
BstV1I GCAGC 4 cut(s) 478, 1717, 1929, 1993
BstV2I GAAGAC 1 cut(s) 1659
BstX2I RGATCY 3 cut(s) 99, 113, 668
BstYI RGATCY 3 cut(s) 99, 113, 668
BstZI CGGCCG 1 cut(s) 349
BsuRI GGCC 9 cut(s) 32, 38, 168, 351, 357, 380, 1050, 1562, 1981
BtgI CCRYGG 2 cut(s) 86, 352
BtgZI GCGATG 2 cut(s) 861, 1923
BtsCI GGATG 7 cut(s) 261, 537, 755, 1161, 1351, 1385, 1574
BtsI GCAGTG 2 cut(s) 630, 2071
BtsIMutI CAGTG 7 cut(s) 129, 588, 630, 1223, 1620, 1739, 2071
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 5 cut(s) 40, 220, 359, 1710, 1983
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 5 cut(s) 75, 393, 649, 1686, 1801
Cfr10I RCCGGY 2 cut(s) 570, 1526
Cfr13I GGNCC 9 cut(s) 37, 106, 166, 370, 378, 1560, 1846, 1922, 1979
Cfr42I CCGCGG 1 cut(s) 355
Cfr9I CCCGGG 1 cut(s) 109
CseI GACGC 4 cut(s) 1029, 1187, 1370, 1934
Csp6I GTAC 4 cut(s) 628, 703, 1720, 1825
CspCI CAANNNNNGTGG 4 cut(s) 1199, 1234, 1751, 1786
CviAII CATG 7 cut(s) 87, 163, 223, 1072, 1313, 1557, 1838
CviQI GTAC 4 cut(s) 628, 703, 1720, 1825
DdeI CTNAG 2 cut(s) 582, 1520
DpnI GATC 7 cut(s) 101, 115, 155, 521, 670, 1350, 1873
DpnII GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
DraI TTTAAA 1 cut(s) 889
DrdI GACNNNNNNGTC 1 cut(s) 613
DseDI GACNNNNNNGTC 1 cut(s) 613
EaeI YGGCCR 2 cut(s) 349, 355
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 4 cut(s) 176, 1113, 1132, 1539
EarI CTCTTC 4 cut(s) 176, 1113, 1132, 1539
EciI GGCGGA 2 cut(s) 225, 589
EclXI CGGCCG 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 86
Eco31I GGTCTC 2 cut(s) 221, 1600
Eco47I GGWCC 4 cut(s) 106, 370, 1846, 1922
Eco52I CGGCCG 1 cut(s) 349
Eco57I CTGAAG 3 cut(s) 1644, 1671, 2012
Eco88I CYCGRG 2 cut(s) 109, 1976
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 331, 374
EcoT14I CCWWGG 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 1768
FaeI CATG 7 cut(s) 90, 166, 226, 1075, 1316, 1560, 1841
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 2 cut(s) 757, 1600
FatI CATG 7 cut(s) 86, 162, 222, 1071, 1312, 1556, 1837
FauI CCCGC 1 cut(s) 47
FauNDI CATATG 2 cut(s) 979, 1367
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 8 cut(s) 30, 352, 355, 426, 492, 1706, 1943, 2007
FokI GGATG 7 cut(s) 248, 524, 742, 1168, 1358, 1372, 1561
Fsp4HI GCNGC 8 cut(s) 30, 352, 355, 426, 492, 1706, 1943, 2007
FspBI CTAG 5 cut(s) 339, 422, 1110, 1878, 2061
FspI TGCGCA 1 cut(s) 74
GlaI GCGC 5 cut(s) 74, 392, 648, 1685, 1800
GluI GCNGC 8 cut(s) 30, 352, 355, 426, 492, 1706, 1943, 2007
GsuI CTGGAG 1 cut(s) 1161
HaeII RGCGCY 2 cut(s) 1687, 1802
HaeIII GGCC 9 cut(s) 32, 38, 168, 351, 357, 380, 1050, 1562, 1981
HapII CCGG 6 cut(s) 46, 110, 571, 1430, 1527, 1592
HgaI GACGC 4 cut(s) 1029, 1187, 1370, 1934
HhaI GCGC 5 cut(s) 75, 393, 649, 1686, 1801
Hin1I GRCGYC 3 cut(s) 909, 1282, 1362
Hin1II CATG 7 cut(s) 90, 166, 226, 1075, 1316, 1560, 1841
Hin6I GCGC 5 cut(s) 73, 391, 647, 1684, 1799
HinP1I GCGC 5 cut(s) 73, 391, 647, 1684, 1799
HincII GTYRAC 1 cut(s) 1169
HindII GTYRAC 1 cut(s) 1169
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 4 cut(s) 466, 1075, 1500, 1672
HpaII CCGG 6 cut(s) 46, 110, 571, 1430, 1527, 1592
HphI GGTGA 6 cut(s) 247, 1025, 1444, 1507, 1541, 1553
Hpy166II GTNNAC 5 cut(s) 7, 434, 628, 803, 1169
Hpy188I TCNGA 6 cut(s) 97, 118, 1080, 1457, 1646, 2031
Hpy188III TCNNGA 7 cut(s) 151, 767, 776, 800, 1072, 1178, 1418
Hpy8I GTNNAC 5 cut(s) 7, 434, 628, 803, 1169
HpyAV CCTTC 9 cut(s) 73, 934, 1339, 1485, 1581, 1598, 1697, 1913, 2026
HpyCH4III ACNGT 5 cut(s) 124, 132, 1166, 1609, 2054
HpyCH4IV ACGT 4 cut(s) 831, 909, 1192, 1282
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 165, 326, 608, 653, 1646, 1692, 1991, 2101
HpyF3I CTNAG 2 cut(s) 582, 1520
HpySE526I ACGT 4 cut(s) 831, 909, 1192, 1282
Hsp92I GRCGYC 3 cut(s) 909, 1282, 1362
Hsp92II CATG 7 cut(s) 90, 166, 226, 1075, 1316, 1560, 1841
HspAI GCGC 5 cut(s) 73, 391, 647, 1684, 1799
Ksp22I TGATCA 1 cut(s) 1348
KspI CCGCGG 1 cut(s) 355
Kzo9I GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
LmnI GCTCC 3 cut(s) 1432, 1478, 1748
Lsp1109I GCAGC 4 cut(s) 478, 1717, 1929, 1993
LweI GCATC 5 cut(s) 168, 255, 838, 1306, 1431
MaeI CTAG 5 cut(s) 339, 422, 1110, 1878, 2061
MaeII ACGT 4 cut(s) 831, 909, 1192, 1282
MaeIII GTNAC 3 cut(s) 124, 253, 1529
MalI GATC 7 cut(s) 101, 115, 155, 521, 670, 1350, 1873
MboI GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
MfeI CAATTG 2 cut(s) 1044, 1961
MflI RGATCY 3 cut(s) 99, 113, 668
MhlI GDGCHC 3 cut(s) 9, 63, 1429
MlyI GAGTC 2 cut(s) 1084, 1681
MmeI TCCRAC 4 cut(s) 75, 646, 692, 798
MseI TTAA 8 cut(s) 366, 693, 888, 1256, 1391, 1565, 1680, 2091
MspA1I CMGCKG 2 cut(s) 354, 491
MspI CCGG 6 cut(s) 46, 110, 571, 1430, 1527, 1592
MspR9I CCNGG 4 cut(s) 110, 111, 333, 376
MunI CAATTG 2 cut(s) 1044, 1961
Mva1269I GAATGC 1 cut(s) 896
MvaI CCWGG 2 cut(s) 333, 376
MvnI CGCG 2 cut(s) 354, 391
MwoI GCNNNNNNNGC 9 cut(s) 35, 165, 326, 608, 653, 1646, 1692, 1991, 2101
NciI CCSGG 2 cut(s) 110, 111
NcoI CCATGG 1 cut(s) 86
NdeI CATATG 2 cut(s) 979, 1367
NdeII GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
NlaIII CATG 7 cut(s) 90, 166, 226, 1075, 1316, 1560, 1841
NlaIV GGNNCC 5 cut(s) 60, 108, 115, 372, 1163
NmeAIII GCCGAG 2 cut(s) 14, 1346
NmuCI GTSAC 3 cut(s) 124, 253, 1529
NsbI TGCGCA 1 cut(s) 74
NspI RCATGY 1 cut(s) 1841
NspV TTCGAA 1 cut(s) 1997
PagI TCATGA 1 cut(s) 1071
PasI CCCWGGG 1 cut(s) 375
PciI ACATGT 1 cut(s) 1837
PcsI WCGNNNNNNNCGW 6 cut(s) 1368, 1899, 1902, 1908, 1911, 2050
PctI GAATGC 1 cut(s) 896
PfeI GAWTC 2 cut(s) 466, 1500
PkrI GCNGC 8 cut(s) 31, 353, 356, 427, 493, 1707, 1944, 2008
PleI GAGTC 2 cut(s) 1083, 1680
PpsI GAGTC 2 cut(s) 1083, 1680
PpuMI RGGWCCY 1 cut(s) 370
PscI ACATGT 1 cut(s) 1837
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 2 cut(s) 331, 374
PspGI CCWGG 2 cut(s) 331, 374
PspN4I GGNNCC 5 cut(s) 60, 108, 115, 372, 1163
PspPI GGNCC 9 cut(s) 37, 106, 166, 370, 378, 1560, 1846, 1922, 1979
PspPPI RGGWCCY 1 cut(s) 370
PstI CTGCAG 1 cut(s) 1707
PsuI RGATCY 3 cut(s) 99, 113, 668
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 4 cut(s) 629, 704, 1721, 1826
RsaNI GTAC 4 cut(s) 628, 703, 1720, 1825
SacII CCGCGG 1 cut(s) 355
SaqAI TTAA 8 cut(s) 366, 693, 888, 1256, 1391, 1565, 1680, 2091
SatI GCNGC 8 cut(s) 30, 352, 355, 426, 492, 1706, 1943, 2007
Sau3AI GATC 7 cut(s) 99, 113, 153, 519, 668, 1348, 1871
Sau96I GGNCC 9 cut(s) 37, 106, 166, 370, 378, 1560, 1846, 1922, 1979
ScaI AGTACT 1 cut(s) 1826
SchI GAGTC 2 cut(s) 1084, 1681
ScrFI CCNGG 4 cut(s) 110, 111, 333, 376
SduI GDGCHC 3 cut(s) 9, 63, 1429
SfaNI GCATC 5 cut(s) 168, 255, 838, 1306, 1431
SfcI CTRYAG 1 cut(s) 1703
Sfr303I CCGCGG 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 1997
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 4 cut(s) 106, 370, 1846, 1922
SmaI CCCGGG 1 cut(s) 111
SmlI CTYRAG 3 cut(s) 767, 1379, 1658
SmoI CTYRAG 3 cut(s) 767, 1379, 1658
SpeI ACTAGT 1 cut(s) 1877
SsiI CCGC 8 cut(s) 29, 40, 210, 265, 352, 354, 426, 574
SspI AATATT 1 cut(s) 938
SspMI CTAG 5 cut(s) 339, 422, 1110, 1878, 2061
StyD4I CCNGG 4 cut(s) 108, 109, 331, 374
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 5 cut(s) 124, 132, 1166, 1609, 2054
TaiI ACGT 4 cut(s) 834, 912, 1195, 1285
TaqI TCGA 5 cut(s) 177, 1467, 1603, 1629, 1997
TaqII GACCGA 1 cut(s) 245
TatI WGTACW 2 cut(s) 627, 1824
TauI GCSGC 4 cut(s) 32, 354, 357, 428
TfiI GAWTC 2 cut(s) 466, 1500
Tru1I TTAA 8 cut(s) 366, 693, 888, 1256, 1391, 1565, 1680, 2091
Tru9I TTAA 8 cut(s) 366, 693, 888, 1256, 1391, 1565, 1680, 2091
TscAI CASTG 7 cut(s) 129, 595, 630, 1223, 1627, 1739, 2071
TseFI GTSAC 3 cut(s) 124, 253, 1529
TseI GCWGC 4 cut(s) 491, 1705, 1942, 2006
Tsp45I GTSAC 3 cut(s) 124, 253, 1529
TspDTI ATGAA 5 cut(s) 916, 1257, 1438, 1563, 1932
TspMI CCCGGG 1 cut(s) 109
TspRI CASTG 7 cut(s) 129, 595, 630, 1223, 1627, 1739, 2071
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 4 cut(s) 106, 370, 1846, 1922
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 4 cut(s) 869, 967, 1258, 1287
XceI RCATGY 1 cut(s) 1841
XmaI CCCGGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 433
XspI CTAG 5 cut(s) 339, 422, 1110, 1878, 2061
ZraI GACGTC 2 cut(s) 910, 1283
ZrmI AGTACT 1 cut(s) 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.