Rroxscaffold_3G00247650

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011205
Physical Location & Seq
Forward (+)
41043213 .. 41047267
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247650.1

Sequence Viewer

Length: 1164 bp
ATGGTCACCGCCTATGACTACCCCTGCGGTTCACCTCGACTCGTCGGAATTAACATTGCCTTGGTCGGCGACTCCGTTGCGATGCGGAAATGGCTCTCAATCTCGTCTTCACCTCACATTCCTCTCTCTTCTCCAAACCCCTCAAACCCCTCACATTTCCAACCCCAAAACCCCAATCCCTCTCTCTACTCTCTCTCTCCTCCCCAAACCCCTAACCCCAAGCTCTCCTCCTTCCGCCCCCGCTCCTCTCTCTCCGACGACACCCCCACAGACGGCGACCGTCCGGCGGCGAAAATCACCGACGAGTGGGGCGAGGCGGCCGAGCCGGAGCCGGAGCCCGAGTCGACAATCTCCGAATCGGACCCTCCGGTGAACGAGGACGAGTGGGGAGGAGCTGATGAGGTCGTGGAAATCGGGAATGGAACTCCGGCCAAAGCTGAGGCTGAGAGCACGGAGGAGGTGGTGGAGGTCGATTCTAAGCTTACTGAGCTGAAGAGGTGCTTGGTAGATACGTTCTATGGGACTGAATTGGGCTTTCGGGCCGGGTCGGAGGTGAGGGCGGAAGTGGTGGAGTTGGTTAACCAGTTGGAGGCGGCGAATCCCACTCCGGCGCCGACGGAGAACCCGGGGCTACTTGATGGCAACTGGGTTTTACTGTACACTGCATCTTCTGAGCTGTTGCCACTTCTGGCAGCAGGTAGCACATCTTTGTTGAAAGTCGATAAAATTAACCAATCAATTGACACCAGCAGTCAGACAATTCTGAACTCCATCACATTGTCCGGCCCTTTTGCCACTTTCTCTTTCAGTGCATCTGCTAGCTTTGAAGTCAGAAGTCCTTCAAGAATCCAGGTTGAATTCAAGGAAGGCACCTTTCAGCCACCAGACATAAAGTCAAGCATTGATCTCCCACAAGATGTGGAAATATTTGGGCAGAGAATCAATTTGTCACCTGTCCAGCAAACCCTCAGTCCTCTGCAGGAGGTTGTTGCGAGTATCTCAAGAACCATCTCTGGTCAGCAATCACTTAAGGTTCCCATTCCAGGTGAGCGGACCCAGTCTTGGCTTCTTACTACGTACCTTGATAGCGATCTCCGAATCTCAAGGGGGGATGGTGGTCTCTTTGTTCTTGCAAAAGAAGGGAGTCCTCTCCTTGATCAGTAA

Protein Analysis

387

Amino Acids

41.63

Weight (kDa)

4.37

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 163 - 378 1.4e-71 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35490
fragaria_vesca FvH4_5g22910 FvH4_5g22910 FvH4_5g22910
malus_domestica MD06G1025700.v1.1 MD16G1280700.v1.1
prunus_persica Prupe.5G029300_v2.0.a1
pyrus_communis pycom06g02090 pycom16g25080
rosa_chinensis RchiOBHm_Chr7g0211641
rosa_laevigata RLG00000002988
rosa_multiflora Rmu_sc0003760.1_g000015 Rmu_sc0004232.1_g000010
rosa_roxburghii Rroxscaffold_3G00247650
rosa_rugosa Rorug07G0126400
rosa_samantha Rh7AG259200 Rh7BG253500 Rh7CG276200 Rh7DG266800
rosa_wichuraiana Rw7G022160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 686
AccB1I GGYRCC 2 cut(s) 610, 869
AccBSI CCGCTC 2 cut(s) 243, 1051
AccI GTMKAC 1 cut(s) 344
AcoI YGGCCR 2 cut(s) 318, 429
AcsI RAATTY 1 cut(s) 857
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 611
AfaI GTAC 2 cut(s) 659, 1079
AfiI CCNNNNNNNGG 6 cut(s) 272, 286, 306, 589, 1043, 1062
AflII CTTAAG 1 cut(s) 1028
AgsI TTSAA 5 cut(s) 715, 827, 843, 857, 862
AhdI GACNNNNNGTC 1 cut(s) 892
AjnI CCWGG 2 cut(s) 849, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 7 cut(s) 223, 395, 437, 481, 490, 676, 822
AluI AGCT 7 cut(s) 223, 395, 437, 481, 490, 676, 822
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 1124
Ama87I CYCGRG 2 cut(s) 338, 625
AoxI GGCC 4 cut(s) 318, 429, 540, 784
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 2 cut(s) 955, 987
Asp700I GAANNNNTTC 1 cut(s) 838
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 4 cut(s) 361, 540, 785, 1053
AsuC2I CCSGG 3 cut(s) 544, 626, 627
AsuHPI GGTGA 7 cut(s) 24, 102, 289, 382, 565, 942, 1058
AsuNHI GCTAGC 1 cut(s) 818
AvaI CYCGRG 2 cut(s) 338, 625
AvaII GGWCC 2 cut(s) 361, 1053
BanI GGYRCC 2 cut(s) 610, 869
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 452
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 704
BccI CCATC 4 cut(s) 632, 779, 1016, 1106
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 2 cut(s) 851, 1044
BclI TGATCA 1 cut(s) 1156
BcnI CCSGG 3 cut(s) 544, 626, 627
BcoDI GTCTC 1 cut(s) 1124
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 977
BfoI RGCGCY 1 cut(s) 614
BfrI CTTAAG 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 4 cut(s) 288, 318, 594, 693
BlsI GCNGC 4 cut(s) 289, 319, 595, 694
Bme1390I CCNGG 5 cut(s) 544, 626, 627, 851, 1044
Bme18I GGWCC 2 cut(s) 361, 1053
BmeRI GACNNNNNGTC 1 cut(s) 892
BmeT110I CYCGRG 2 cut(s) 338, 625
BmgT120I GGNCC 4 cut(s) 361, 540, 785, 1053
BmiI GGNNCC 7 cut(s) 330, 336, 363, 612, 871, 1035, 1055
BmrFI CCNGG 5 cut(s) 544, 626, 627, 851, 1044
BmrI ACTGGG 2 cut(s) 655, 1051
BmsI GCATC 3 cut(s) 72, 674, 821
BmtI GCTAGC 1 cut(s) 822
BmuI ACTGGG 2 cut(s) 655, 1051
BpiI GAAGAC 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 2 cut(s) 985, 1087
BpuMI CCSGG 3 cut(s) 544, 626, 627
BsaAI YACGTR 1 cut(s) 1077
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 1 cut(s) 611
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 3 cut(s) 60, 625, 626
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 6 cut(s) 272, 286, 306, 589, 1043, 1062
Bse1I ACTGG 3 cut(s) 583, 650, 1057
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 2 cut(s) 851, 1044
BseDI CCNNGG 3 cut(s) 60, 625, 626
BseGI GGATG 1 cut(s) 1117
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 6 cut(s) 272, 286, 306, 589, 1043, 1062
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 5 cut(s) 429, 435, 477, 663, 982
BseNI ACTGG 3 cut(s) 583, 650, 1057
BseRI GAGGAG 5 cut(s) 189, 217, 235, 405, 470
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 704
Bsh1285I CGRYCG 2 cut(s) 280, 321
BshFI GGCC 4 cut(s) 320, 431, 542, 786
BshNI GGYRCC 2 cut(s) 610, 869
BsiEI CGRYCG 2 cut(s) 280, 321
BsiHKAI GWGCWC 1 cut(s) 452
BsiHKCI CYCGRG 2 cut(s) 338, 625
BsiSI CCGG 9 cut(s) 284, 326, 332, 368, 428, 543, 608, 626, 783
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 6 cut(s) 272, 286, 306, 589, 1043, 1062
BsmAI GTCTC 1 cut(s) 1124
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 4 cut(s) 320, 431, 542, 786
Bso31I GGTCTC 1 cut(s) 1124
BsoBI CYCGRG 2 cut(s) 338, 625
Bsp1286I GDGCHC 2 cut(s) 339, 452
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 3 cut(s) 904, 1090, 1156
BspANI GGCC 4 cut(s) 320, 431, 542, 786
BspCNI CTCAG 5 cut(s) 430, 436, 478, 664, 981
BspLI GGNNCC 7 cut(s) 330, 336, 363, 612, 871, 1035, 1055
BspMAI CTGCAG 1 cut(s) 981
BspMI ACCTGC 1 cut(s) 686
BspOI GCTAGC 1 cut(s) 822
BspT107I GGYRCC 2 cut(s) 610, 869
BspTI CTTAAG 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 1124
BsrBI CCGCTC 2 cut(s) 243, 1051
BsrDI GCAATG 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 3 cut(s) 583, 650, 1057
BssECI CCNNGG 3 cut(s) 60, 625, 626
BssMI GATC 3 cut(s) 904, 1090, 1156
BssNI GRCGYC 1 cut(s) 611
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 851, 1044
Bst4CI ACNGT 2 cut(s) 281, 657
Bst6I CTCTTC 2 cut(s) 133, 488
BstACI GRCGYC 1 cut(s) 611
BstAFI CTTAAG 1 cut(s) 1028
BstAUI TGTACA 1 cut(s) 657
BstBAI YACGTR 1 cut(s) 1077
BstC8I GCNNGC 1 cut(s) 820
BstDEI CTNAG 6 cut(s) 438, 444, 477, 486, 672, 968
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 1117
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 3 cut(s) 907, 1093, 1159
BstMAI GTCTC 1 cut(s) 1124
BstMBI GATC 3 cut(s) 904, 1090, 1156
BstMCI CGRYCG 2 cut(s) 280, 321
BstMWI GCNNNNNNNGC 2 cut(s) 91, 487
BstNI CCWGG 2 cut(s) 851, 1044
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 5 cut(s) 542, 624, 625, 849, 1042
BstSFI CTRYAG 1 cut(s) 977
BstSNI TACGTA 1 cut(s) 1077
BstV1I GCAGC 1 cut(s) 704
BstV2I GAAGAC 1 cut(s) 99
BstZI CGGCCG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 320, 431, 542, 786
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 1117
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 2 cut(s) 660, 814
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 1 cut(s) 820
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 4 cut(s) 361, 540, 785, 1053
Cfr9I CCCGGG 1 cut(s) 625
Csp6I GTAC 2 cut(s) 658, 1078
CviQI GTAC 2 cut(s) 658, 1078
DdeI CTNAG 6 cut(s) 438, 444, 477, 486, 672, 968
DinI GGCGCC 1 cut(s) 612
DpnI GATC 3 cut(s) 906, 1092, 1158
DpnII GATC 3 cut(s) 904, 1090, 1156
DriI GACNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 2 cut(s) 318, 429
EagI CGGCCG 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 133, 488
Eam1105I GACNNNNNGTC 1 cut(s) 892
EarI CTCTTC 2 cut(s) 133, 488
EciI GGCGGA 2 cut(s) 224, 575
EclXI CGGCCG 1 cut(s) 318
Eco105I TACGTA 1 cut(s) 1077
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 1124
Eco47I GGWCC 2 cut(s) 361, 1053
Eco52I CGGCCG 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 2 cut(s) 338, 625
Eco91I GGTNACC 1 cut(s) 4
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 849, 1042
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 339
EgeI GGCGCC 1 cut(s) 612
EheI GGCGCC 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 60
FaiI YATR 3 cut(s) 15, 519, 890
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 1 cut(s) 535
FauI CCCGC 1 cut(s) 248
FbaI TGATCA 1 cut(s) 1156
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 288, 318, 594, 693
FokI GGATG 1 cut(s) 1124
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 288, 318, 594, 693
FspBI CTAG 1 cut(s) 819
GlaI GCGC 1 cut(s) 612
GluI GCNGC 4 cut(s) 288, 318, 594, 693
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 4 cut(s) 320, 431, 542, 786
HapII CCGG 9 cut(s) 284, 326, 332, 368, 428, 543, 608, 626, 783
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 611
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HincII GTYRAC 2 cut(s) 345, 580
HindII GTYRAC 2 cut(s) 345, 580
HindIII AAGCTT 1 cut(s) 479
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 9 cut(s) 284, 326, 332, 368, 428, 543, 608, 626, 783
HphI GGTGA 7 cut(s) 24, 102, 289, 382, 565, 942, 1058
Hpy166II GTNNAC 5 cut(s) 32, 345, 373, 580, 660
Hpy188III TCNNGA 3 cut(s) 415, 843, 1002
Hpy8I GTNNAC 5 cut(s) 32, 345, 373, 580, 660
Hpy99I CGWCG 4 cut(s) 47, 260, 305, 619
HpyAV CCTTC 4 cut(s) 241, 849, 860, 1133
HpyCH4III ACNGT 2 cut(s) 281, 657
HpyCH4IV ACGT 2 cut(s) 512, 1076
HpyCH4V TGCA 4 cut(s) 665, 812, 979, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 487
HpyF3I CTNAG 6 cut(s) 438, 444, 477, 486, 672, 968
HpySE526I ACGT 2 cut(s) 512, 1076
Hsp92I GRCGYC 1 cut(s) 611
HspAI GCGC 1 cut(s) 611
KasI GGCGCC 1 cut(s) 610
Ksp22I TGATCA 1 cut(s) 1156
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 3 cut(s) 904, 1090, 1156
LmnI GCTCC 4 cut(s) 248, 328, 334, 392
Lsp1109I GCAGC 1 cut(s) 704
LweI GCATC 3 cut(s) 72, 674, 821
MaeI CTAG 1 cut(s) 819
MaeII ACGT 2 cut(s) 512, 1076
MaeIII GTNAC 2 cut(s) 4, 948
MalI GATC 3 cut(s) 906, 1092, 1158
MbiI CCGCTC 2 cut(s) 243, 1051
MboI GATC 3 cut(s) 904, 1090, 1156
MboII GAAGA 4 cut(s) 99, 120, 505, 660
MfeI CAATTG 1 cut(s) 738
MhlI GDGCHC 2 cut(s) 339, 452
MluCI AATT 7 cut(s) 48, 527, 726, 738, 759, 857, 943
Mly113I GGCGCC 1 cut(s) 611
MlyI GAGTC 4 cut(s) 33, 65, 350, 1153
MmeI TCCRAC 5 cut(s) 25, 184, 279, 528, 567
MroXI GAANNNNTTC 1 cut(s) 838
MseI TTAA 4 cut(s) 51, 579, 729, 1029
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 9 cut(s) 284, 326, 332, 368, 428, 543, 608, 626, 783
MspR9I CCNGG 5 cut(s) 544, 626, 627, 851, 1044
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 2 cut(s) 851, 1044
MwoI GCNNNNNNNGC 2 cut(s) 91, 487
NarI GGCGCC 1 cut(s) 611
NciI CCSGG 3 cut(s) 544, 626, 627
NdeII GATC 3 cut(s) 904, 1090, 1156
NheI GCTAGC 1 cut(s) 818
NlaIV GGNNCC 7 cut(s) 330, 336, 363, 612, 871, 1035, 1055
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 2 cut(s) 4, 948
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 309
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 6 cut(s) 356, 473, 598, 846, 939, 1098
PflFI GACNNNGTC 1 cut(s) 1057
PkrI GCNGC 4 cut(s) 289, 319, 595, 694
PleI GAGTC 4 cut(s) 33, 65, 349, 1152
PluTI GGCGCC 1 cut(s) 614
PpsI GAGTC 4 cut(s) 33, 65, 349, 1152
Ppu21I YACGTR 1 cut(s) 1077
Psp6I CCWGG 2 cut(s) 849, 1042
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 849, 1042
PspN4I GGNNCC 7 cut(s) 330, 336, 363, 612, 871, 1035, 1055
PspPI GGNCC 4 cut(s) 361, 540, 785, 1053
PstI CTGCAG 1 cut(s) 981
PsyI GACNNNGTC 1 cut(s) 1057
RsaI GTAC 2 cut(s) 659, 1079
RsaNI GTAC 2 cut(s) 658, 1078
SalI GTCGAC 1 cut(s) 343
SaqAI TTAA 4 cut(s) 51, 579, 729, 1029
SatI GCNGC 4 cut(s) 288, 318, 594, 693
Sau3AI GATC 3 cut(s) 904, 1090, 1156
Sau96I GGNCC 4 cut(s) 361, 540, 785, 1053
SchI GAGTC 4 cut(s) 33, 65, 350, 1153
ScrFI CCNGG 5 cut(s) 544, 626, 627, 851, 1044
SduI GDGCHC 2 cut(s) 339, 452
SfaNI GCATC 3 cut(s) 72, 674, 821
SfcI CTRYAG 1 cut(s) 977
SfoI GGCGCC 1 cut(s) 612
SinI GGWCC 2 cut(s) 361, 1053
SmaI CCCGGG 1 cut(s) 627
SmlI CTYRAG 3 cut(s) 1000, 1028, 1102
SmoI CTYRAG 3 cut(s) 1000, 1028, 1102
SnaBI TACGTA 1 cut(s) 1077
Sse9I AATT 7 cut(s) 48, 527, 726, 738, 759, 857, 943
SspDI GGCGCC 1 cut(s) 610
SspI AATATT 1 cut(s) 927
SspMI CTAG 1 cut(s) 819
StyD4I CCNGG 5 cut(s) 542, 624, 625, 849, 1042
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 281, 657
TaiI ACGT 2 cut(s) 515, 1079
TaqI TCGA 4 cut(s) 37, 344, 471, 720
TasI AATT 7 cut(s) 48, 527, 726, 738, 759, 857, 943
TatI WGTACW 1 cut(s) 657
TauI GCSGC 3 cut(s) 290, 320, 596
TfiI GAWTC 6 cut(s) 356, 473, 598, 846, 939, 1098
Tru1I TTAA 4 cut(s) 51, 579, 729, 1029
Tru9I TTAA 4 cut(s) 51, 579, 729, 1029
TscAI CASTG 2 cut(s) 667, 814
TseFI GTSAC 2 cut(s) 4, 948
TseI GCWGC 1 cut(s) 692
Tsp45I GTSAC 2 cut(s) 4, 948
TspGWI ACGGA 3 cut(s) 64, 467, 632
TspMI CCCGGG 1 cut(s) 625
TspRI CASTG 2 cut(s) 667, 814
Tth111I GACNNNGTC 1 cut(s) 1057
Vha464I CTTAAG 1 cut(s) 1028
VpaK11BI GGWCC 2 cut(s) 361, 1053
XapI RAATTY 1 cut(s) 857
XmaI CCCGGG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.