Rw7G022160

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011205
Physical Location & Seq
Reverse (-)
25969662 .. 25972909
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022160.1

Sequence Viewer

Length: 1071 bp
ATGGCTCTCAATCTCGTCTTCACCTCACACTCTTCTCTCTTCTCCAAACCCCTCACATTTCCAACCCCAAAACCCCAATCCCTCTCACTACTCTCTCTCTCTCTCTCCTCCCCAAACCCTAACCCCAAGCTCTCCTCCTTCCGCCCCCGCTCCTCTCTCTCCGACGACACCCCCACCGACGGCGACCGTCCGGCGGCGAAAATCACCGACGAGTGGGGCGAGGCGGCCGAGCCGGAGCCCGAGCCCGAGTCGACAATCTCCGAATCGGACCCTCCGGTGAACGAGGACGAGTGGGGAGGAGCTGATGAGGCCGTGGAAATCGGGAATGGAACTCCGGCCAAAGCTGAGGCTGAGAGCACGGAAGAGGTGGAGGAGGTCGGTTCTAAGCTTACTGAGCTGAAGAGGTGCTTGGTAGATACGTTCTATGGGACTGAATTGGGCTTTCGGGCCGGGTCGGAGGTGAGAGCGGAAGTGGTGGAGTTGGTTAACCAGTTGGAGGCGGCGAATCCCACTCCGGCGCCGACGGAGAACCCGGGGCTACTTGATGGCAACTGGGTTTTACTGTACACCGCATCTTCTGAGCTGTTGCCACTTCTGGCAGCAGGTAGCACATCTTTGTTGAAAGTCGATAAAATTAACCAATCAATTGACACCAGCAGTCAGAAAATTCTGAACTCCATCACATTGTCCGGCCCTTTTGCCACTTTCTCTTTCAGTGCATCTGCTAGCTTTGAAGTCAGAAGTCCTTCAAGAATCCAGGTTGAATTCAAGGAAGGCACCTTTCAGCCACCAGACATAAAGTCAAGCATTGATCTCCCACAAGATGTGGAAATATTTGGGCAGAGAATCAATTTGTCACCTGTCCAGCAAACTCTCAGTCCTCTGCAGGAGGTTGTTGCGAGTATCTCAAGAACCATCTCTGGTCAGCAATCACTTAAGGTTCCCATTCCAGGTGAGCGGACCCAGTCTTGGCTCCTTACTACGTACCTTGATGGTGATCTACGAATCTCAAGGGGGGATGGTGGTCTCTTTGTTCTTGCAAAAGAAGGGAGTCCTCTCCTTGATCAGTAA

Protein Analysis

356

Amino Acids

38.24

Weight (kDa)

4.4

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 132 - 347 1.7e-71 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35490
fragaria_vesca FvH4_5g22910 FvH4_5g22910 FvH4_5g22910
malus_domestica MD06G1025700.v1.1 MD16G1280700.v1.1
prunus_persica Prupe.5G029300_v2.0.a1
pyrus_communis pycom06g02090 pycom16g25080
rosa_chinensis RchiOBHm_Chr7g0211641
rosa_laevigata RLG00000002988
rosa_multiflora Rmu_sc0003760.1_g000015 Rmu_sc0004232.1_g000010
rosa_roxburghii Rroxscaffold_3G00247650
rosa_rugosa Rorug07G0126400
rosa_samantha Rh7AG259200 Rh7BG253500 Rh7CG276200 Rh7DG266800
rosa_wichuraiana Rw7G022160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 517, 776
AccBSI CCGCTC 3 cut(s) 150, 467, 958
AccI GTMKAC 1 cut(s) 251
AciI CCGC 8 cut(s) 142, 148, 194, 224, 467, 500, 570, 958
AcoI YGGCCR 2 cut(s) 225, 336
AcsI RAATTY 2 cut(s) 666, 764
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 566, 986
AfiI CCNNNNNNNGG 6 cut(s) 179, 193, 213, 496, 950, 969
AflII CTTAAG 1 cut(s) 935
AgsI TTSAA 5 cut(s) 622, 734, 750, 764, 769
AhdI GACNNNNNGTC 1 cut(s) 799
AjnI CCWGG 2 cut(s) 756, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 7 cut(s) 130, 302, 344, 388, 397, 583, 729
AluI AGCT 7 cut(s) 130, 302, 344, 388, 397, 583, 729
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 1031
Ama87I CYCGRG 3 cut(s) 239, 245, 532
AoxI GGCC 5 cut(s) 225, 309, 336, 447, 691
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 2 cut(s) 666, 764
ArsI GACNNNNNNTTYG 2 cut(s) 862, 894
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 4 cut(s) 268, 447, 692, 960
AsuC2I CCSGG 3 cut(s) 451, 533, 534
AsuHPI GGTGA 7 cut(s) 13, 196, 289, 472, 849, 965, 1007
AsuNHI GCTAGC 1 cut(s) 725
AvaI CYCGRG 3 cut(s) 239, 245, 532
AvaII GGWCC 2 cut(s) 268, 960
BanI GGYRCC 2 cut(s) 517, 776
BanII GRGCYC 2 cut(s) 240, 246
BbsI GAAGAC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 359
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 611
BccI CCATC 5 cut(s) 539, 686, 923, 986, 1013
BceAI ACGGC 2 cut(s) 196, 296
BciT130I CCWGG 2 cut(s) 758, 951
BclI TGATCA 1 cut(s) 1063
BcnI CCSGG 3 cut(s) 451, 533, 534
BcoDI GTCTC 1 cut(s) 1031
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 884
BfoI RGCGCY 1 cut(s) 521
BfrI CTTAAG 1 cut(s) 935
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 4 cut(s) 195, 225, 501, 600
BlsI GCNGC 4 cut(s) 196, 226, 502, 601
Bme1390I CCNGG 5 cut(s) 451, 533, 534, 758, 951
Bme18I GGWCC 2 cut(s) 268, 960
BmeRI GACNNNNNGTC 1 cut(s) 799
BmeT110I CYCGRG 3 cut(s) 239, 245, 532
BmgT120I GGNCC 4 cut(s) 268, 447, 692, 960
BmiI GGNNCC 7 cut(s) 237, 270, 519, 778, 942, 962, 974
BmrFI CCNGG 5 cut(s) 451, 533, 534, 758, 951
BmrI ACTGGG 2 cut(s) 562, 958
BmsI GCATC 2 cut(s) 581, 728
BmtI GCTAGC 1 cut(s) 729
BmuI ACTGGG 2 cut(s) 562, 958
BpiI GAAGAC 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 892, 994
BpuMI CCSGG 3 cut(s) 451, 533, 534
BsaAI YACGTR 1 cut(s) 984
BsaBI GATNNNNATC 1 cut(s) 996
BsaHI GRCGYC 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 3 cut(s) 312, 532, 533
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 6 cut(s) 179, 193, 213, 496, 950, 969
Bse1I ACTGG 3 cut(s) 490, 557, 964
Bse8I GATNNNNATC 1 cut(s) 996
BseBI CCWGG 2 cut(s) 758, 951
BseDI CCNNGG 3 cut(s) 312, 532, 533
BseGI GGATG 1 cut(s) 1024
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 6 cut(s) 179, 193, 213, 496, 950, 969
BseMII CTCAG 5 cut(s) 336, 342, 384, 570, 889
BseNI ACTGG 3 cut(s) 490, 557, 964
BseRI GAGGAG 5 cut(s) 97, 124, 142, 312, 386
BseX3I CGGCCG 1 cut(s) 225
BseXI GCAGC 1 cut(s) 611
Bsh1285I CGRYCG 2 cut(s) 187, 228
BshFI GGCC 5 cut(s) 227, 311, 338, 449, 693
BshNI GGYRCC 2 cut(s) 517, 776
BsiEI CGRYCG 2 cut(s) 187, 228
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 3 cut(s) 239, 245, 532
BsiSI CCGG 8 cut(s) 191, 233, 275, 335, 450, 515, 533, 690
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 6 cut(s) 179, 193, 213, 496, 950, 969
BsmAI GTCTC 1 cut(s) 1031
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 5 cut(s) 227, 311, 338, 449, 693
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 3 cut(s) 239, 245, 532
Bsp1286I GDGCHC 3 cut(s) 240, 246, 359
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 811, 997, 1063
BspACI CCGC 8 cut(s) 142, 148, 194, 224, 467, 500, 570, 958
BspANI GGCC 5 cut(s) 227, 311, 338, 449, 693
BspCNI CTCAG 5 cut(s) 337, 343, 385, 571, 888
BspLI GGNNCC 7 cut(s) 237, 270, 519, 778, 942, 962, 974
BspMAI CTGCAG 1 cut(s) 888
BspMI ACCTGC 1 cut(s) 593
BspOI GCTAGC 1 cut(s) 729
BspT107I GGYRCC 2 cut(s) 517, 776
BspTI CTTAAG 1 cut(s) 935
BspTNI GGTCTC 1 cut(s) 1031
BsrBI CCGCTC 3 cut(s) 150, 467, 958
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 3 cut(s) 490, 557, 964
BssECI CCNNGG 3 cut(s) 312, 532, 533
BssMI GATC 3 cut(s) 811, 997, 1063
BssNI GRCGYC 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 758, 951
Bst4CI ACNGT 2 cut(s) 188, 564
Bst6I CTCTTC 4 cut(s) 37, 44, 357, 395
BstACI GRCGYC 1 cut(s) 518
BstAFI CTTAAG 1 cut(s) 935
BstAUI TGTACA 1 cut(s) 564
BstBAI YACGTR 1 cut(s) 984
BstC8I GCNNGC 1 cut(s) 727
BstDEI CTNAG 6 cut(s) 345, 351, 384, 393, 579, 875
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 1024
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 3 cut(s) 814, 1000, 1066
BstMAI GTCTC 1 cut(s) 1031
BstMBI GATC 3 cut(s) 811, 997, 1063
BstMCI CGRYCG 2 cut(s) 187, 228
BstMWI GCNNNNNNNGC 2 cut(s) 308, 394
BstNI CCWGG 2 cut(s) 758, 951
BstSCI CCNGG 5 cut(s) 449, 531, 532, 756, 949
BstSFI CTRYAG 1 cut(s) 884
BstSNI TACGTA 1 cut(s) 984
BstV1I GCAGC 1 cut(s) 611
BstV2I GAAGAC 1 cut(s) 10
BstZI CGGCCG 1 cut(s) 225
BsuRI GGCC 5 cut(s) 227, 311, 338, 449, 693
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 1024
BtsIMutI CAGTG 1 cut(s) 721
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 727
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 4 cut(s) 268, 447, 692, 960
Cfr9I CCCGGG 1 cut(s) 532
Csp6I GTAC 2 cut(s) 565, 985
CviQI GTAC 2 cut(s) 565, 985
DdeI CTNAG 6 cut(s) 345, 351, 384, 393, 579, 875
DinI GGCGCC 1 cut(s) 519
DpnI GATC 3 cut(s) 813, 999, 1065
DpnII GATC 3 cut(s) 811, 997, 1063
DriI GACNNNNNGTC 1 cut(s) 799
EaeI YGGCCR 2 cut(s) 225, 336
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 4 cut(s) 37, 44, 357, 395
Eam1105I GACNNNNNGTC 1 cut(s) 799
EarI CTCTTC 4 cut(s) 37, 44, 357, 395
EciI GGCGGA 1 cut(s) 131
EclXI CGGCCG 1 cut(s) 225
Eco105I TACGTA 1 cut(s) 984
Eco24I GRGCYC 2 cut(s) 240, 246
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 2 cut(s) 268, 960
Eco52I CGGCCG 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 3 cut(s) 239, 245, 532
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 756, 949
EcoT38I GRGCYC 2 cut(s) 240, 246
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
FaiI YATR 2 cut(s) 426, 797
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 442
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 4 cut(s) 195, 225, 501, 600
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 2 cut(s) 240, 246
Fsp4HI GCNGC 4 cut(s) 195, 225, 501, 600
FspBI CTAG 1 cut(s) 726
GlaI GCGC 1 cut(s) 519
GluI GCNGC 4 cut(s) 195, 225, 501, 600
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 5 cut(s) 227, 311, 338, 449, 693
HapII CCGG 8 cut(s) 191, 233, 275, 335, 450, 515, 533, 690
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 518
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 2 cut(s) 252, 487
HindII GTYRAC 2 cut(s) 252, 487
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 7 cut(s) 248, 263, 505, 753, 846, 1005, 1051
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 8 cut(s) 191, 233, 275, 335, 450, 515, 533, 690
HphI GGTGA 7 cut(s) 13, 196, 289, 472, 849, 965, 1007
Hpy166II GTNNAC 4 cut(s) 252, 280, 487, 567
Hpy188I TCNGA 8 cut(s) 163, 262, 268, 457, 580, 663, 672, 740
Hpy188III TCNNGA 3 cut(s) 322, 750, 909
Hpy8I GTNNAC 4 cut(s) 252, 280, 487, 567
Hpy99I CGWCG 4 cut(s) 167, 182, 212, 526
HpyAV CCTTC 4 cut(s) 148, 756, 767, 1040
HpyCH4III ACNGT 2 cut(s) 188, 564
HpyCH4IV ACGT 2 cut(s) 419, 983
HpyCH4V TGCA 3 cut(s) 719, 886, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 394
HpyF3I CTNAG 6 cut(s) 345, 351, 384, 393, 579, 875
HpySE526I ACGT 2 cut(s) 419, 983
Hsp92I GRCGYC 1 cut(s) 518
HspAI GCGC 1 cut(s) 518
KasI GGCGCC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 1063
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 3 cut(s) 811, 997, 1063
LmnI GCTCC 4 cut(s) 155, 235, 299, 978
Lsp1109I GCAGC 1 cut(s) 611
LweI GCATC 2 cut(s) 581, 728
MaeI CTAG 1 cut(s) 726
MaeII ACGT 2 cut(s) 419, 983
MaeIII GTNAC 1 cut(s) 855
MalI GATC 3 cut(s) 813, 999, 1065
MbiI CCGCTC 3 cut(s) 150, 467, 958
MboI GATC 3 cut(s) 811, 997, 1063
MboII GAAGA 6 cut(s) 10, 24, 31, 374, 412, 567
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 3 cut(s) 240, 246, 359
MluCI AATT 6 cut(s) 434, 633, 645, 666, 764, 850
Mly113I GGCGCC 1 cut(s) 518
MlyI GAGTC 2 cut(s) 257, 1060
MmeI TCCRAC 4 cut(s) 86, 186, 435, 474
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 3 cut(s) 486, 636, 936
MspCI CTTAAG 1 cut(s) 935
MspI CCGG 8 cut(s) 191, 233, 275, 335, 450, 515, 533, 690
MspR9I CCNGG 5 cut(s) 451, 533, 534, 758, 951
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 2 cut(s) 758, 951
MwoI GCNNNNNNNGC 2 cut(s) 308, 394
NarI GGCGCC 1 cut(s) 518
NciI CCSGG 3 cut(s) 451, 533, 534
NdeII GATC 3 cut(s) 811, 997, 1063
NheI GCTAGC 1 cut(s) 725
NlaIV GGNNCC 7 cut(s) 237, 270, 519, 778, 942, 962, 974
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 855
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 5 cut(s) 263, 505, 753, 846, 1005
PflFI GACNNNGTC 1 cut(s) 964
PkrI GCNGC 4 cut(s) 196, 226, 502, 601
PleI GAGTC 2 cut(s) 256, 1059
PluTI GGCGCC 1 cut(s) 521
PpsI GAGTC 2 cut(s) 256, 1059
Ppu21I YACGTR 1 cut(s) 984
Psp6I CCWGG 2 cut(s) 756, 949
PspGI CCWGG 2 cut(s) 756, 949
PspN4I GGNNCC 7 cut(s) 237, 270, 519, 778, 942, 962, 974
PspPI GGNCC 4 cut(s) 268, 447, 692, 960
PstI CTGCAG 1 cut(s) 888
PsyI GACNNNGTC 1 cut(s) 964
RsaI GTAC 2 cut(s) 566, 986
RsaNI GTAC 2 cut(s) 565, 985
SalI GTCGAC 1 cut(s) 250
SaqAI TTAA 3 cut(s) 486, 636, 936
SatI GCNGC 4 cut(s) 195, 225, 501, 600
Sau3AI GATC 3 cut(s) 811, 997, 1063
Sau96I GGNCC 4 cut(s) 268, 447, 692, 960
SchI GAGTC 2 cut(s) 257, 1060
ScrFI CCNGG 5 cut(s) 451, 533, 534, 758, 951
SduI GDGCHC 3 cut(s) 240, 246, 359
SfaNI GCATC 2 cut(s) 581, 728
SfcI CTRYAG 1 cut(s) 884
SfoI GGCGCC 1 cut(s) 519
SinI GGWCC 2 cut(s) 268, 960
SmaI CCCGGG 1 cut(s) 534
SmlI CTYRAG 3 cut(s) 907, 935, 1009
SmoI CTYRAG 3 cut(s) 907, 935, 1009
SnaBI TACGTA 1 cut(s) 984
Sse9I AATT 6 cut(s) 434, 633, 645, 666, 764, 850
SsiI CCGC 8 cut(s) 142, 148, 194, 224, 467, 500, 570, 958
SspDI GGCGCC 1 cut(s) 517
SspI AATATT 1 cut(s) 834
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 5 cut(s) 449, 531, 532, 756, 949
TaaI ACNGT 2 cut(s) 188, 564
TaiI ACGT 2 cut(s) 422, 986
TaqI TCGA 2 cut(s) 251, 627
TasI AATT 6 cut(s) 434, 633, 645, 666, 764, 850
TatI WGTACW 1 cut(s) 564
TauI GCSGC 3 cut(s) 197, 227, 503
TfiI GAWTC 5 cut(s) 263, 505, 753, 846, 1005
Tru1I TTAA 3 cut(s) 486, 636, 936
Tru9I TTAA 3 cut(s) 486, 636, 936
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 1 cut(s) 855
TspGWI ACGGA 2 cut(s) 374, 539
TspMI CCCGGG 1 cut(s) 532
TspRI CASTG 1 cut(s) 721
Tth111I GACNNNGTC 1 cut(s) 964
Vha464I CTTAAG 1 cut(s) 935
VpaK11BI GGWCC 2 cut(s) 268, 960
XapI RAATTY 2 cut(s) 666, 764
XmaI CCCGGG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.