Rh7DG266700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011857
Physical Location & Seq
Reverse (-)
28333120 .. 28336410
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG266700.1

Sequence Viewer

Length: 492 bp
ATGAGCAACAAGCAGCCAGTGAAGCTAGACGACGACCAAATCGCCGACCTCCGCGAGATATTCCGGTCGTTTGACCGGAACAACGACGGCAGCCTGACCCAGCTGGAGCTGGGCTCGCTGTTACGTTCACTTGGGCTGAAGCCGGGACCGGAGCAGCTGGATTCCTTGATCCAGAAGGCAGACACTAACAGCAATGGCCTGGTGGAGTTCTCGGAGTTCGTGGCGCTGGTGGCGCCGGAGCTGCTCTCGGCTAAGTCCCCCTACACGGAGGACCAGCTGAGGCAGCTGTTCAGGATGTTTGATAGGGATGGCAACGGGTATATCACCGCGGCGGAGTTGGCTCACTCCATGGCCAAATTGGGGCATGCGCTCACGGCAGAGGAGCTCACGGGGATGATCAGGGAGGCAGATACTGACGGTGATGGGAGGATCAATTTTCAGGAGTTTGCTCACGCTATCACTTCTGCTGCTTTTGATAATTCTTGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

163

Amino Acids

18.01

Weight (kDa)

4.54

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 16 - 77 2.3e-16 EF-hand domain pair
EF-hand_1 PF00036 16 - 44 7.4e-06 EF hand domain
EF-hand_6 PF13405 16 - 45 6.1e-08 EF-hand domain
EF-hand_7 PF13499 90 - 153 8.4e-18 EF-hand domain pair
EF-hand_6 PF13405 92 - 121 3.7e-08 EF-hand domain
EF-hand_1 PF00036 93 - 120 2.4e-07 EF hand domain
EF-hand_8 PF13833 105 - 150 1.9e-11 EF-hand domain pair
EF-hand_1 PF00036 130 - 155 1.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 232
AccII CGCG 2 cut(s) 54, 329
AciI CCGC 4 cut(s) 52, 327, 329, 332
AclWI GGATC 2 cut(s) 163, 437
AcoI YGGCCR 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 265, 360
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 8 cut(s) 25, 103, 109, 157, 241, 277, 286, 385
AluI AGCT 8 cut(s) 25, 103, 109, 157, 241, 277, 286, 385
Alw21I GWGCWC 1 cut(s) 387
AlwI GGATC 2 cut(s) 163, 437
AlwNI CAGNNNCTG 1 cut(s) 413
AoxI GGCC 2 cut(s) 196, 351
ApeKI GCWGC 6 cut(s) 13, 90, 154, 241, 283, 467
AspLEI GCGC 3 cut(s) 226, 235, 370
AspS9I GGNCC 2 cut(s) 146, 271
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 316, 431
AvaII GGWCC 2 cut(s) 146, 271
BalI TGGCCA 1 cut(s) 353
BanI GGYRCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 116, 387
Bbv12I GWGCWC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 6 cut(s) 25, 102, 166, 228, 295, 454
BccI CCATC 2 cut(s) 302, 416
BceAI ACGGC 2 cut(s) 103, 390
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 144
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 2 cut(s) 227, 236
BisI GCNGC 7 cut(s) 14, 91, 155, 242, 284, 330, 468
BlsI GCNGC 7 cut(s) 15, 92, 156, 243, 285, 331, 469
Bme1390I CCNGG 2 cut(s) 144, 200
Bme18I GGWCC 2 cut(s) 146, 271
BmgT120I GGNCC 2 cut(s) 146, 271
BmiI GGNNCC 2 cut(s) 147, 234
BmrFI CCNGG 2 cut(s) 144, 200
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 230, 262
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 327, 348
BsaWI WCCGGW 3 cut(s) 63, 75, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 360
Bse1I ACTGG 1 cut(s) 17
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 327, 348
BseGI GGATG 3 cut(s) 300, 313, 399
BseLI CCNNNNNNNGG 2 cut(s) 265, 360
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 6 cut(s) 25, 102, 166, 228, 295, 454
BseYI CCCAGC 2 cut(s) 99, 109
Bsh1236I CGCG 2 cut(s) 54, 329
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 198, 353
BshNI GGYRCC 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 5 cut(s) 64, 76, 143, 149, 236
BslFI GGGAC 2 cut(s) 159, 241
BslI CCNNNNNNNGG 2 cut(s) 265, 360
BsmFI GGGAC 2 cut(s) 159, 241
BsnI GGCC 2 cut(s) 198, 353
Bsp1286I GDGCHC 2 cut(s) 116, 387
Bsp143I GATC 3 cut(s) 168, 396, 429
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 4 cut(s) 52, 327, 329, 332
BspANI GGCC 2 cut(s) 198, 353
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 2 cut(s) 54, 329
BspLI GGNNCC 2 cut(s) 147, 234
BspPI GGATC 2 cut(s) 163, 437
BspT107I GGYRCC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 327, 348
BssMI GATC 3 cut(s) 168, 396, 429
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 116, 366
BstDEI CTNAG 2 cut(s) 252, 278
BstDSI CCRYGG 2 cut(s) 327, 348
BstF5I GGATG 3 cut(s) 300, 313, 399
BstFNI CGCG 2 cut(s) 54, 329
BstH2I RGCGCY 2 cut(s) 227, 236
BstHHI GCGC 3 cut(s) 226, 235, 370
BstKTI GATC 3 cut(s) 171, 399, 432
BstMBI GATC 3 cut(s) 168, 396, 429
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 8 cut(s) 22, 115, 230, 232, 241, 283, 338, 374
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 142, 198
BstUI CGCG 2 cut(s) 54, 329
BstV1I GCAGC 6 cut(s) 25, 102, 166, 228, 295, 454
BsuRI GGCC 2 cut(s) 198, 353
BtgI CCRYGG 2 cut(s) 327, 348
BtsCI GGATG 3 cut(s) 300, 313, 399
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 116, 366
CaiI CAGNNNCTG 1 cut(s) 413
CfoI GCGC 3 cut(s) 226, 235, 370
Cfr13I GGNCC 2 cut(s) 146, 271
Cfr42I CCGCGG 1 cut(s) 330
CviAII CATG 2 cut(s) 349, 365
DdeI CTNAG 2 cut(s) 252, 278
DinI GGCGCC 1 cut(s) 234
DpnI GATC 3 cut(s) 170, 398, 431
DpnII GATC 3 cut(s) 168, 396, 429
EaeI YGGCCR 1 cut(s) 351
EciI GGCGGA 1 cut(s) 347
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 2 cut(s) 116, 387
Eco47I GGWCC 2 cut(s) 146, 271
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 2 cut(s) 116, 387
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 2 cut(s) 352, 368
FaiI YATR 3 cut(s) 321, 350, 366
FaqI GGGAC 2 cut(s) 159, 241
FatI CATG 2 cut(s) 348, 364
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 7 cut(s) 14, 91, 155, 242, 284, 330, 468
FokI GGATG 3 cut(s) 307, 320, 406
FriOI GRGCYC 2 cut(s) 116, 387
Fsp4HI GCNGC 7 cut(s) 14, 91, 155, 242, 284, 330, 468
FspBI CTAG 1 cut(s) 26
GlaI GCGC 3 cut(s) 225, 234, 369
GluI GCNGC 7 cut(s) 14, 91, 155, 242, 284, 330, 468
GsaI CCCAGC 2 cut(s) 103, 113
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 2 cut(s) 227, 236
HaeIII GGCC 2 cut(s) 198, 353
HapII CCGG 5 cut(s) 64, 76, 143, 149, 236
HhaI GCGC 3 cut(s) 226, 235, 370
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 2 cut(s) 352, 368
Hin6I GCGC 3 cut(s) 224, 233, 368
HinP1I GCGC 3 cut(s) 224, 233, 368
HinfI GANTC 1 cut(s) 161
HpaII CCGG 5 cut(s) 64, 76, 143, 149, 236
HphI GGTGA 2 cut(s) 316, 431
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 3 cut(s) 172, 292, 440
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 2 cut(s) 35, 89
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 115, 230, 232, 241, 283, 338, 374
HpyF3I CTNAG 2 cut(s) 252, 278
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 2 cut(s) 352, 368
HspAI GCGC 3 cut(s) 224, 233, 368
KasI GGCGCC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 396
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 3 cut(s) 168, 396, 429
LmnI GCTCC 4 cut(s) 106, 151, 238, 382
Lsp1109I GCAGC 6 cut(s) 25, 102, 166, 228, 295, 454
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 1 cut(s) 120
MalI GATC 3 cut(s) 170, 398, 431
MboI GATC 3 cut(s) 168, 396, 429
MhlI GDGCHC 2 cut(s) 116, 387
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 3 cut(s) 356, 433, 478
MluNI TGGCCA 1 cut(s) 353
Mly113I GGCGCC 1 cut(s) 233
MnlI CCTC 6 cut(s) 59, 262, 273, 373, 397, 420
Mox20I TGGCCA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 353
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 5 cut(s) 103, 157, 277, 286, 329
MspI CCGG 5 cut(s) 64, 76, 143, 149, 236
MspR9I CCNGG 2 cut(s) 144, 200
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 2 cut(s) 54, 329
MwoI GCNNNNNNNGC 8 cut(s) 22, 115, 230, 232, 241, 283, 338, 374
NarI GGCGCC 1 cut(s) 233
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 348
NdeII GATC 3 cut(s) 168, 396, 429
NlaIII CATG 2 cut(s) 352, 368
NlaIV GGNNCC 2 cut(s) 147, 234
NmeAIII GCCGAG 1 cut(s) 227
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 7 cut(s) 15, 92, 156, 243, 285, 331, 469
PluTI GGCGCC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 198
PspFI CCCAGC 2 cut(s) 99, 109
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 147, 234
PspPI GGNCC 2 cut(s) 146, 271
PstNI CAGNNNCTG 1 cut(s) 413
PvuII CAGCTG 4 cut(s) 103, 157, 277, 286
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 387
SacII CCGCGG 1 cut(s) 330
SatI GCNGC 7 cut(s) 14, 91, 155, 242, 284, 330, 468
Sau3AI GATC 3 cut(s) 168, 396, 429
Sau96I GGNCC 2 cut(s) 146, 271
ScrFI CCNGG 2 cut(s) 144, 200
SduI GDGCHC 2 cut(s) 116, 387
SfoI GGCGCC 1 cut(s) 234
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 2 cut(s) 146, 271
SmiMI CAYNNNNRTG 1 cut(s) 392
SphI GCATGC 1 cut(s) 368
Sse9I AATT 3 cut(s) 356, 433, 478
SsiI CCGC 4 cut(s) 52, 327, 329, 332
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 142, 198
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 1 cut(s) 127
TasI AATT 3 cut(s) 356, 433, 478
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 161
TscAI CASTG 1 cut(s) 24
TseI GCWGC 6 cut(s) 13, 90, 154, 241, 283, 467
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 146, 271
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.