MD06G1025600.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011857
Physical Location & Seq
Reverse (-)
3086180 .. 3086671
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1025600.v1.1.491

Sequence Viewer

Length: 492 bp
ATGAGCAACAAGCAGCCAGTGAAGCTGGACGACGACCAAATCGCGGACCTGCGGGAAATATTCCGCTCGTTTGATCGAAACAACGACGGAAGCCTAACGCAGCTGGAGCTGGGGTCGCTTCTCCGCTCACTGGGGTTGAAGCCGGGGCCGGAGCAGCTCGATGCCTTGATCCAGAAGGCAGACACAAACAGCAATGGCCTCGTGGAGTTTTCCGAGTTCGTAGGGCTGGTAGCGCCGGAGCTTCTCTCAGCCAAGTCTCCTTACACGGAGGACCAGCTGAGGCAGTTGTTTCGGATGTTCGATAGGGACGGCAATGGATTCATCACGGCAGCTGAGCTGGCGCACTCCATGGCGAAACTGGGGCACGCTCTTACGGCGGAGGAGTTGACAGGGATGATCAAGGAGGCGGATACGGATGGCGATGGAAGAATCAATTTCCAGGAGTTTGCTCACGCCATTACTTCCGCCGCTTTCGATAATTCTTGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

164

Amino Acids

17.95

Weight (kDa)

4.54

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 16 - 76 4.4e-16 EF-hand domain pair
EF-hand_1 PF00036 16 - 44 7.4e-06 EF hand domain
EF-hand_6 PF13405 16 - 45 6.1e-08 EF-hand domain
EF-hand_7 PF13499 90 - 153 1.2e-17 EF-hand domain pair
EF-hand_6 PF13405 92 - 121 8.1e-08 EF-hand domain
EF-hand_1 PF00036 93 - 120 3.8e-07 EF hand domain
EF-hand_8 PF13833 106 - 150 3.5e-10 EF-hand domain pair
EF-hand_1 PF00036 130 - 155 1.3e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 57
AccBSI CCGCTC 2 cut(s) 66, 126
AccII CGCG 1 cut(s) 44
AciI CCGC 8 cut(s) 44, 52, 64, 124, 377, 407, 465, 468
AclWI GGATC 1 cut(s) 163
AfiI CCNNNNNNNGG 2 cut(s) 43, 130
AgsI TTSAA 1 cut(s) 139
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 8 cut(s) 25, 103, 109, 157, 241, 277, 332, 337
AluI AGCT 8 cut(s) 25, 103, 109, 157, 241, 277, 332, 337
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 1 cut(s) 163
AoxI GGCC 2 cut(s) 146, 196
ApeKI GCWGC 4 cut(s) 13, 100, 154, 329
AspLEI GCGC 2 cut(s) 235, 343
AspS9I GGNCC 3 cut(s) 46, 146, 271
AsuC2I CCSGG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 46, 271
BaeGI GKGCMC 1 cut(s) 366
BauI CACGAG 1 cut(s) 200
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 25, 112, 166, 341
BccI CCATC 2 cut(s) 410, 416
BceAI ACGGC 3 cut(s) 325, 342, 390
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 272, 306
BciT130I CCWGG 1 cut(s) 440
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 236
BfuAI ACCTGC 1 cut(s) 57
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 5 cut(s) 14, 101, 155, 330, 468
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 5 cut(s) 15, 102, 156, 331, 469
Bme1390I CCNGG 2 cut(s) 144, 440
Bme18I GGWCC 2 cut(s) 46, 271
BmgT120I GGNCC 3 cut(s) 46, 146, 271
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 2 cut(s) 144, 440
BmrI ACTGGG 2 cut(s) 140, 368
BmsI GCATC 1 cut(s) 151
BmuI ACTGGG 2 cut(s) 140, 368
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 143, 348
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 130
Bse1I ACTGG 3 cut(s) 17, 135, 363
Bse3DI GCAATG 2 cut(s) 199, 319
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 143, 348
BseGI GGATG 3 cut(s) 300, 399, 421
BseLI CCNNNNNNNGG 2 cut(s) 43, 130
BseMI GCAATG 2 cut(s) 199, 319
BseMII CTCAG 3 cut(s) 261, 269, 324
BseNI ACTGG 3 cut(s) 17, 135, 363
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 4 cut(s) 25, 112, 166, 341
BseYI CCCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 148, 198
BsiSI CCGG 3 cut(s) 143, 149, 236
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 43, 130
BsmAI GTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 2 cut(s) 148, 198
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 3 cut(s) 73, 168, 396
Bsp1720I GCTNAGC 1 cut(s) 333
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 8 cut(s) 44, 52, 64, 124, 377, 407, 465, 468
BspANI GGCC 2 cut(s) 148, 198
BspCNI CTCAG 3 cut(s) 260, 270, 325
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 57
BspPI GGATC 1 cut(s) 163
BsrBI CCGCTC 2 cut(s) 66, 126
BsrDI GCAATG 2 cut(s) 199, 319
BsrI ACTGG 3 cut(s) 17, 135, 363
BssECI CCNNGG 2 cut(s) 143, 348
BssMI GATC 3 cut(s) 73, 168, 396
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 440
BstC8I GCNNGC 2 cut(s) 339, 366
BstDEI CTNAG 4 cut(s) 247, 278, 333, 489
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 3 cut(s) 300, 399, 421
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 2 cut(s) 235, 343
BstKTI GATC 3 cut(s) 76, 171, 399
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 3 cut(s) 73, 168, 396
BstMWI GCNNNNNNNGC 7 cut(s) 22, 106, 115, 154, 232, 338, 374
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 142, 438
BstSLI GKGCMC 1 cut(s) 366
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 4 cut(s) 25, 112, 166, 341
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 2 cut(s) 148, 198
BtgI CCRYGG 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 300, 399, 421
BtsIMutI CAGTG 2 cut(s) 24, 128
BveI ACCTGC 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 339, 366
CfoI GCGC 2 cut(s) 235, 343
Cfr13I GGNCC 3 cut(s) 46, 146, 271
CviAII CATG 1 cut(s) 349
DdeI CTNAG 4 cut(s) 247, 278, 333, 489
DpnI GATC 3 cut(s) 75, 170, 398
DpnII GATC 3 cut(s) 73, 168, 396
EciI GGCGGA 3 cut(s) 392, 422, 454
Eco130I CCWWGG 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 46, 271
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 1 cut(s) 352
FaiI YATR 1 cut(s) 350
FaqI GGGAC 1 cut(s) 320
FatI CATG 1 cut(s) 348
FauI CCCGC 1 cut(s) 45
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 5 cut(s) 14, 101, 155, 330, 468
FokI GGATG 3 cut(s) 307, 406, 428
Fsp4HI GCNGC 5 cut(s) 14, 101, 155, 330, 468
GlaI GCGC 2 cut(s) 234, 342
GluI GCNGC 5 cut(s) 14, 101, 155, 330, 468
GsaI CCCAGC 1 cut(s) 113
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 2 cut(s) 148, 198
HapII CCGG 3 cut(s) 143, 149, 236
HhaI GCGC 2 cut(s) 235, 343
Hin1II CATG 1 cut(s) 352
Hin6I GCGC 2 cut(s) 233, 341
HinP1I GCGC 2 cut(s) 233, 341
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 2 cut(s) 318, 429
HpaII CCGG 3 cut(s) 143, 149, 236
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 2 cut(s) 214, 294
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 2 cut(s) 35, 89
HpyAV CCTTC 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 106, 115, 154, 232, 338, 374
HpyF3I CTNAG 4 cut(s) 247, 278, 333, 489
Hsp92II CATG 1 cut(s) 352
HspAI GCGC 2 cut(s) 233, 341
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 3 cut(s) 73, 168, 396
LmnI GCTCC 3 cut(s) 106, 151, 238
Lsp1109I GCAGC 4 cut(s) 25, 112, 166, 341
LweI GCATC 1 cut(s) 151
MalI GATC 3 cut(s) 75, 170, 398
MbiI CCGCTC 2 cut(s) 66, 126
MboI GATC 3 cut(s) 73, 168, 396
MboII GAAGA 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 2 cut(s) 433, 478
MnlI CCTC 5 cut(s) 209, 262, 273, 373, 397
MspA1I CMGCKG 3 cut(s) 103, 277, 332
MspI CCGG 3 cut(s) 143, 149, 236
MspR9I CCNGG 2 cut(s) 144, 440
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 7 cut(s) 22, 106, 115, 154, 232, 338, 374
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 348
NdeII GATC 3 cut(s) 73, 168, 396
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 147
PfeI GAWTC 2 cut(s) 318, 429
PfoI TCCNGGA 1 cut(s) 438
PkrI GCNGC 5 cut(s) 15, 102, 156, 331, 469
Psp6I CCWGG 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 46, 146, 271
PvuII CAGCTG 3 cut(s) 103, 277, 332
SatI GCNGC 5 cut(s) 14, 101, 155, 330, 468
Sau3AI GATC 3 cut(s) 73, 168, 396
Sau96I GGNCC 3 cut(s) 46, 146, 271
ScrFI CCNGG 2 cut(s) 144, 440
SduI GDGCHC 1 cut(s) 366
SetI ASST 9 cut(s) 27, 51, 105, 111, 159, 243, 279, 334, 339
SfaNI GCATC 1 cut(s) 151
SinI GGWCC 2 cut(s) 46, 271
Sse9I AATT 2 cut(s) 433, 478
SsiI CCGC 8 cut(s) 44, 52, 64, 124, 377, 407, 465, 468
SspI AATATT 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 142, 438
StyI CCWWGG 1 cut(s) 348
TaqI TCGA 4 cut(s) 76, 159, 300, 474
TasI AATT 2 cut(s) 433, 478
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 2 cut(s) 318, 429
TscAI CASTG 2 cut(s) 24, 135
TseI GCWGC 4 cut(s) 13, 100, 154, 329
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 3 cut(s) 102, 281, 428
TspRI CASTG 2 cut(s) 24, 135
VpaK11BI GGWCC 2 cut(s) 46, 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.