FvH4_2g21960

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011857
Physical Location & Seq
Forward (+)
18125390 .. 18126619
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21960.t1

Sequence Viewer

Length: 492 bp
ATGAGCAACAAGCAGCCAGTGAAGCTAGACGACGACCAAATAGCCGACCTCCGCGAGATATTTCGGTCGTTTGACCGGAACAACGACGGCAGCCTGACCCAGCTGGAGCTGGGATCGCTGTTACGTTCACTTGGGTTGAAGCCGGGACCAGATCAGCTGGATACCTTGATCCAGAAGGCAGACACTAATAGTAATGGTCTGGTGGAGTTCTCGGAGTTCGTGGCGCTGGTGGCGCCGGAGCTGGTCTCGGCTAAGTCACCCTACACGGAGGACCAGCTGAGGCAGTTGTTCAGGATGTTTGACAGGGATGGCAACGGCTTTATCACCGCGGCGGAGTTGGCTCACTCCATGGCCAAATTAGGGCATGCCCTCACGGCAGAGGAGCTGACGGGGATGATCAGGGAGGCGGATACAGACGGTGATGGGAGGATCAATTTTCAGGAGTTTGCTCACGCTATCACTTCTGCCGCTTTTGATAATTCTTGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

164

Amino Acids

17.98

Weight (kDa)

4.53

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 16 - 77 3.4e-16 EF-hand domain pair
EF-hand_1 PF00036 16 - 44 7.4e-06 EF hand domain
EF-hand_6 PF13405 16 - 45 6.1e-08 EF-hand domain
EF-hand_7 PF13499 90 - 153 1.5e-17 EF-hand domain pair
EF-hand_6 PF13405 92 - 121 8.1e-08 EF-hand domain
EF-hand_1 PF00036 93 - 120 3.8e-07 EF hand domain
EF-hand_8 PF13833 106 - 150 1.9e-10 EF-hand domain pair
EF-hand_1 PF00036 130 - 155 1.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 232
AccII CGCG 2 cut(s) 54, 329
AciI CCGC 6 cut(s) 52, 327, 329, 332, 407, 468
AclWI GGATC 3 cut(s) 121, 163, 437
AcoI YGGCCR 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 360
AgsI TTSAA 1 cut(s) 139
AluBI AGCT 7 cut(s) 25, 103, 109, 157, 241, 277, 385
AluI AGCT 7 cut(s) 25, 103, 109, 157, 241, 277, 385
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 3 cut(s) 121, 163, 437
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 2 cut(s) 13, 90
AspLEI GCGC 2 cut(s) 226, 235
AspS9I GGNCC 2 cut(s) 146, 271
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 249, 316, 431
AvaII GGWCC 2 cut(s) 146, 271
BalI TGGCCA 1 cut(s) 353
BanI GGYRCC 1 cut(s) 232
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 25, 102
BccI CCATC 2 cut(s) 302, 416
BceAI ACGGC 3 cut(s) 103, 331, 390
BciVI GTATCC 2 cut(s) 154, 403
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 2 cut(s) 227, 236
BfuI GTATCC 2 cut(s) 154, 403
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 4 cut(s) 14, 91, 330, 468
BlsI GCNGC 4 cut(s) 15, 92, 331, 469
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 146, 271
BmgT120I GGNCC 2 cut(s) 146, 271
BmiI GGNNCC 2 cut(s) 147, 234
BmrFI CCNGG 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 327, 348
BsaWI WCCGGW 1 cut(s) 75
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse1I ACTGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 327, 348
BseGI GGATG 3 cut(s) 300, 313, 399
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 25, 102
BseYI CCCAGC 2 cut(s) 99, 109
Bsh1236I CGCG 2 cut(s) 54, 329
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 353
BshNI GGYRCC 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 68
BsiSI CCGG 3 cut(s) 76, 143, 236
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 1 cut(s) 353
Bso31I GGTCTC 1 cut(s) 250
Bsp143I GATC 5 cut(s) 113, 151, 168, 396, 429
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 6 cut(s) 52, 327, 329, 332, 407, 468
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 2 cut(s) 54, 329
BspLI GGNNCC 2 cut(s) 147, 234
BspPI GGATC 3 cut(s) 121, 163, 437
BspT107I GGYRCC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 250
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 327, 348
BssMI GATC 5 cut(s) 113, 151, 168, 396, 429
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 252, 278
BstDSI CCRYGG 2 cut(s) 327, 348
BstF5I GGATG 3 cut(s) 300, 313, 399
BstFNI CGCG 2 cut(s) 54, 329
BstH2I RGCGCY 2 cut(s) 227, 236
BstHHI GCGC 2 cut(s) 226, 235
BstKTI GATC 5 cut(s) 116, 154, 171, 399, 432
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 5 cut(s) 113, 151, 168, 396, 429
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 6 cut(s) 22, 115, 230, 232, 338, 374
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 2 cut(s) 54, 329
BstV1I GCAGC 2 cut(s) 25, 102
BsuI GTATCC 2 cut(s) 154, 403
BsuRI GGCC 1 cut(s) 353
BtgI CCRYGG 2 cut(s) 327, 348
BtsCI GGATG 3 cut(s) 300, 313, 399
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 366
CfoI GCGC 2 cut(s) 226, 235
Cfr13I GGNCC 2 cut(s) 146, 271
Cfr42I CCGCGG 1 cut(s) 330
CviAII CATG 2 cut(s) 349, 365
DdeI CTNAG 2 cut(s) 252, 278
DinI GGCGCC 1 cut(s) 234
DpnI GATC 5 cut(s) 115, 153, 170, 398, 431
DpnII GATC 5 cut(s) 113, 151, 168, 396, 429
EaeI YGGCCR 1 cut(s) 351
EciI GGCGGA 2 cut(s) 347, 422
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 146, 271
EcoT14I CCWWGG 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 2 cut(s) 352, 368
FaiI YATR 2 cut(s) 350, 366
FaqI GGGAC 1 cut(s) 159
FatI CATG 2 cut(s) 348, 364
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 4 cut(s) 14, 91, 330, 468
FokI GGATG 3 cut(s) 307, 320, 406
Fsp4HI GCNGC 4 cut(s) 14, 91, 330, 468
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 225, 234
GluI GCNGC 4 cut(s) 14, 91, 330, 468
GsaI CCCAGC 2 cut(s) 103, 113
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 2 cut(s) 227, 236
HaeIII GGCC 1 cut(s) 353
HapII CCGG 3 cut(s) 76, 143, 236
HhaI GCGC 2 cut(s) 226, 235
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 2 cut(s) 352, 368
Hin6I GCGC 2 cut(s) 224, 233
HinP1I GCGC 2 cut(s) 224, 233
HpaII CCGG 3 cut(s) 76, 143, 236
HphI GGTGA 3 cut(s) 249, 316, 431
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 3 cut(s) 172, 292, 440
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 2 cut(s) 35, 89
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 115, 230, 232, 338, 374
HpyF3I CTNAG 2 cut(s) 252, 278
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 2 cut(s) 352, 368
HspAI GCGC 2 cut(s) 224, 233
KasI GGCGCC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 396
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 5 cut(s) 113, 151, 168, 396, 429
LmnI GCTCC 3 cut(s) 106, 238, 382
Lsp1109I GCAGC 2 cut(s) 25, 102
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 120, 255
MalI GATC 5 cut(s) 115, 153, 170, 398, 431
MboI GATC 5 cut(s) 113, 151, 168, 396, 429
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 3 cut(s) 356, 433, 478
MluNI TGGCCA 1 cut(s) 353
Mly113I GGCGCC 1 cut(s) 233
MnlI CCTC 7 cut(s) 59, 262, 273, 373, 380, 397, 420
Mox20I TGGCCA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 353
MseI TTAA 1 cut(s) 490
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 4 cut(s) 103, 157, 277, 329
MspI CCGG 3 cut(s) 76, 143, 236
MspR9I CCNGG 1 cut(s) 144
MvnI CGCG 2 cut(s) 54, 329
MwoI GCNNNNNNNGC 6 cut(s) 22, 115, 230, 232, 338, 374
NarI GGCGCC 1 cut(s) 233
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 348
NdeII GATC 5 cut(s) 113, 151, 168, 396, 429
NlaIII CATG 2 cut(s) 352, 368
NlaIV GGNNCC 2 cut(s) 147, 234
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 255
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PkrI GCNGC 4 cut(s) 15, 92, 331, 469
PluTI GGCGCC 1 cut(s) 236
PspFI CCCAGC 2 cut(s) 99, 109
PspN4I GGNNCC 2 cut(s) 147, 234
PspPI GGNCC 2 cut(s) 146, 271
PvuII CAGCTG 3 cut(s) 103, 157, 277
SacII CCGCGG 1 cut(s) 330
SaqAI TTAA 1 cut(s) 490
SatI GCNGC 4 cut(s) 14, 91, 330, 468
Sau3AI GATC 5 cut(s) 113, 151, 168, 396, 429
Sau96I GGNCC 2 cut(s) 146, 271
ScrFI CCNGG 1 cut(s) 144
SfoI GGCGCC 1 cut(s) 234
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 2 cut(s) 146, 271
SphI GCATGC 1 cut(s) 368
Sse9I AATT 3 cut(s) 356, 433, 478
SsiI CCGC 6 cut(s) 52, 327, 329, 332, 407, 468
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 1 cut(s) 127
TaqII GACCGA 1 cut(s) 54
TasI AATT 3 cut(s) 356, 433, 478
TauI GCSGC 2 cut(s) 332, 470
Tru1I TTAA 1 cut(s) 490
Tru9I TTAA 1 cut(s) 490
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 13, 90
Tsp45I GTSAC 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 146, 271
XceI RCATGY 1 cut(s) 368
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.