Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012707

Physical Location & Seq

Genomic Coordinates Forward (+)

20098522 .. 20100978

Gene Structure 2457 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G53830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCACCAATCAAAGAATTCATATCCAAATTCTCAGATTTCAAAAACAACAAGAAACTCATTCTCTCCTCCGCCGCAATAGCCTTGCTATTATTGGCCTCCATAGTCGGAATCGCCGCCACAACAACAAACCAAAACAAAAACCAAAAAATCACAACTCTATCTTCGACATCACACGCCATTCTAAAATCTGTTTGCAGCTCAACACTATACCCGGAGCTCTGTTTTTCCGCCGTGGCAGCCACCGGAGGAAAAGAGCTGACGTCACAGAAAGAAGTCATCGAAGCGTCACTTAACCTAACTACCAAAGCCGTGAAACACAACTACTTCGCTGTCAAGAAGCTGATCGCGAAGAGAAAAGGTTTGACACCGCGTGAGGTCACGGCGCTTCACGACTGTTTGGAGACAATCGACGAGACGTTAGATGAGCTCCACGTGGCGGTGGAGGATCTTCATCAGTATCCTAAGCAAAAATCTCTGAGGAAACATGCCGATGATCTCAAAACACTAATCAGCTCAGCCATTACTAATCAAGGGACTTGCCTTGACGGTTTCTCTTACGACGACGCCGATAGGAAAGTTCGTAAGGCGTTATTGAAGGGTCAGGTACACGTGGAGCACATGTGTAGCAACGCATTAGCAATGATCAAGAACATGACAGAAACCGACATAGCCAACTTTGAGCTAAGAGATAAGTCTTCAACCTTCACCAACAACAACAACCGCAAGCTCAAGGAGGTTACCGGCGACTTAGACAGTGATGGATGGCCAAAGTGGTTATCCGTTGGAGACAGAAGGCTTCTTCAAGGGTCAACAATTAAAGCAGACGCTACGGTGGCGGATGACGGAAGCGGTGACTTTACAACGGTAGCCGCGGCTGTGGCTGCGGCGCCAGAGAAGAGTAACAAGAGGTTTGTGATACATATAAAGGCAGGAGTTTATAGAGAGAATGTGGAAGTGACGAAGAAGAAGACGAATATAATGTTCTTGGGAGATGGCCGTGGCAAAACTATTATCACTGGAAGTAGAAATGTCGTCGATGGAAGCACCACTTTCCACTCCGCTACTGTTGCTGCCGTCGGCGAGAGGTTTCTAGCGCGTGACATCACATTCCAAAACACCGCCGGTCCGTCGAAGCATCAAGCGGTGGCTCTCCGAGTCGGATCAGATTTTTCCGCTTTTTACCAATGCGACATGTTTGCTTATCAAGACACACTTTATGTCCACTCAAATCGTCAATTCTTTGTGAAATGTCACATAACCGGAACCGTCGATTTCATCTTCGGAAACGCAGCCGCGGTTTTACAAGACTGCGATATAAATGCACGCAGACCAAATTCCGGTCAGAAAAACATGGTTACCGCCCAAGGACGGTCTGATCCGAATCAAAACACGGGGATTGTGATCCAGAATTGTCGTATTGGTGGCACGTCGGATCTTTTGGCCGTGAAAGGAACGTTCCCGACGTATCTTGGTCGTCCTTGGAAGGAATATTCACGGACGGTGATAATGCAATCGGATATATCCGACGTGATCCGACCCGAAGGATGGCATGAGTGGAGCGGGAGTTTTGCGTTGGACACTCTAACTTATAGAGAGTATTTGAATAGAGGAGGAGGGGCAGGAACTGCAAATAGAGTGAAGTGGAAGGGCTATAAGGTAATTACAAGTGATACAGAAGCTCAGCCATTTACGGCTGGTCAGTTTATTGGTGGTGGAGGCTGGTTAGCGTCGACCGGTTTTCCTTTCTCGTTAAGTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0008150 GO:0009505 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

587

Amino Acids

64.17

Weight (kDa)

9.08

Isoelectric Point (pI)

24.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	61 - 215	3.3e-33	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	276 - 572	1.7e-149	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012707)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53830 AT3G14310
fragaria_vesca	FvH4_6g35830
malus_domestica	MD09G1172600.v1.1 MD17G1154500.v1.1
prunus_persica	Prupe.3G031000_v2.0.a1
pyrus_communis	pycom09g09010 pycom09g09020
rosa_chinensis	RchiOBHm_Chr2g0147881
rosa_laevigata	RLG00000020287
rosa_multiflora	Rmu_sc0000308.1_g000057
rosa_roxburghii	Rroxscaffold_2G00099240
rosa_rugosa	Rorug02G0402600
rosa_samantha	Rh2AG459500 Rh2BG472900 Rh2CG446900 Rh2DG481800
rosa_wichuraiana	Rw2G037590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	266
AccB1I	GGYRCC	2 cut(s)	4, 889
AccBSI	CCGCTC	1 cut(s)	1563
AccI	GTMKAC	1 cut(s)	1733
AccII	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
AclI	AACGTT	1 cut(s)	1457
AclWI	GGATC	6 cut(s)	456, 1171, 1373, 1399, 1443, 1528
AcoI	YGGCCR	3 cut(s)	767, 997, 1443
AcsI	RAATTY	3 cut(s)	17, 29, 1336
AcvI	CACGTG	2 cut(s)	436, 613
AcyI	GRCGYC	3 cut(s)	263, 567, 890
AdeI	CACNNNGTG	1 cut(s)	374
AfaI	GTAC	1 cut(s)	609
AfiI	CCNNNNNNNGG	4 cut(s)	439, 1340, 1371, 1548
AflIII	ACRYGT	3 cut(s)	610, 621, 1194
AgeI	ACCGGT	1 cut(s)	1736
AgsI	TTSAA	5 cut(s)	43, 598, 702, 806, 1606
AjiI	CACGTC	2 cut(s)	1431, 1531
AluBI	AGCT	9 cut(s)	201, 220, 259, 343, 430, 516, 685, 730, 1682
AluI	AGCT	9 cut(s)	201, 220, 259, 343, 430, 516, 685, 730, 1682
Alw21I	GWGCWC	3 cut(s)	222, 432, 621
Alw26I	GTCTC	3 cut(s)	398, 410, 783
AlwI	GGATC	6 cut(s)	456, 1171, 1373, 1399, 1443, 1528
AlwNI	CAGNNNCTG	1 cut(s)	1628
AoxI	GGCC	4 cut(s)	96, 767, 997, 1443
ApeKI	GCWGC	5 cut(s)	198, 239, 884, 1073, 1292
ApoI	RAATTY	3 cut(s)	17, 29, 1336
AsiGI	ACCGGT	1 cut(s)	1736
AspLEI	GCGC	3 cut(s)	388, 892, 1099
AspS9I	GGNCC	1 cut(s)	1127
AsuC2I	CCSGG	1 cut(s)	215
AsuHPI	GGTGA	3 cut(s)	700, 866, 1516
AvaII	GGWCC	1 cut(s)	1127
BalI	TGGCCA	1 cut(s)	769
BanI	GGYRCC	2 cut(s)	4, 889
BanII	GRGCYC	2 cut(s)	222, 432
BbrPI	CACGTG	2 cut(s)	436, 613
BbsI	GAAGAC	2 cut(s)	690, 977
Bbv12I	GWGCWC	3 cut(s)	222, 432, 621
BbvI	GCAGC	5 cut(s)	210, 251, 871, 1060, 1304
BccI	CCATC	5 cut(s)	755, 759, 989, 1034, 1542
BceAI	ACGGC	7 cut(s)	218, 296, 399, 984, 1061, 1430, 1710
BcgI	CGANNNNNNTGC	4 cut(s)	1181, 1215, 1304, 1338
BciVI	GTATCC	1 cut(s)	471
BclI	TGATCA	1 cut(s)	645
BcnI	CCSGG	1 cut(s)	215
BcoDI	GTCTC	3 cut(s)	398, 410, 783
BfaI	CTAG	1 cut(s)	1094
BfoI	RGCGCY	2 cut(s)	389, 893
BfuI	GTATCC	1 cut(s)	471
BlpI	GCTNAGC	2 cut(s)	517, 1683
Bme1390I	CCNGG	1 cut(s)	215
Bme18I	GGWCC	1 cut(s)	1127
BmgBI	CACGTC	2 cut(s)	1431, 1531
BmgT120I	GGNCC	1 cut(s)	1127
BmiI	GGNNCC	3 cut(s)	6, 891, 1267
BmrFI	CCNGG	1 cut(s)	215
BmsI	GCATC	1 cut(s)	1147
BpiI	GAAGAC	2 cut(s)	690, 977
Bpu10I	CCTNAGC	1 cut(s)	465
Bpu1102I	GCTNAGC	2 cut(s)	517, 1683
BpuEI	CTTGAG	1 cut(s)	716
BpuMI	CCSGG	1 cut(s)	215
BsaAI	YACGTR	2 cut(s)	436, 613
BsaBI	GATNNNNATC	3 cut(s)	453, 1383, 1403
BsaHI	GRCGYC	3 cut(s)	263, 567, 890
BsaJI	CCNNGG	6 cut(s)	234, 873, 1000, 1296, 1366, 1481
BsaWI	WCCGGW	4 cut(s)	245, 1262, 1340, 1736
BsaXI	ACNNNNNCTCC	4 cut(s)	608, 638, 1552, 1582
Bsc4I	CCNNNNNNNGG	4 cut(s)	439, 1340, 1371, 1548
Bse118I	RCCGGY	3 cut(s)	743, 1124, 1736
Bse1I	ACTGG	1 cut(s)	1024
Bse3DI	GCAATG	1 cut(s)	648
Bse8I	GATNNNNATC	3 cut(s)	453, 1383, 1403
BseDI	CCNNGG	6 cut(s)	234, 873, 1000, 1296, 1366, 1481
BseGI	GGATG	3 cut(s)	770, 847, 1553
BseJI	GATNNNNATC	3 cut(s)	453, 1383, 1403
BseLI	CCNNNNNNNGG	4 cut(s)	439, 1340, 1371, 1548
BseMI	GCAATG	1 cut(s)	648
BseMII	CTCAG	4 cut(s)	48, 470, 531, 1697
BseNI	ACTGG	1 cut(s)	1024
BseRI	GAGGAG	3 cut(s)	58, 1626, 1629
BseXI	GCAGC	5 cut(s)	210, 251, 871, 1060, 1304
Bsh1236I	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
Bsh1285I	CGRYCG	1 cut(s)	1737
BshFI	GGCC	4 cut(s)	98, 769, 999, 1445
BshNI	GGYRCC	2 cut(s)	4, 889
BshTI	ACCGGT	1 cut(s)	1736
BsiEI	CGRYCG	1 cut(s)	1737
BsiHKAI	GWGCWC	3 cut(s)	222, 432, 621
BsiSI	CCGG	7 cut(s)	215, 246, 744, 1125, 1263, 1341, 1737
BslFI	GGGAC	1 cut(s)	550
BslI	CCNNNNNNNGG	4 cut(s)	439, 1340, 1371, 1548
BsmAI	GTCTC	3 cut(s)	398, 410, 783
BsmBI	CGTCTC	1 cut(s)	410
BsmFI	GGGAC	1 cut(s)	550
BsnI	GGCC	4 cut(s)	98, 769, 999, 1445
Bsp1286I	GDGCHC	3 cut(s)	222, 432, 621
Bsp143I	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
Bsp1720I	GCTNAGC	2 cut(s)	517, 1683
Bsp68I	TCGCGA	1 cut(s)	350
BspANI	GGCC	4 cut(s)	98, 769, 999, 1445
BspCNI	CTCAG	4 cut(s)	47, 471, 530, 1696
BspFNI	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
BspLI	GGNNCC	3 cut(s)	6, 891, 1267
BspPI	GGATC	6 cut(s)	456, 1171, 1373, 1399, 1443, 1528
BspT107I	GGYRCC	2 cut(s)	4, 889
BsrBI	CCGCTC	1 cut(s)	1563
BsrDI	GCAATG	1 cut(s)	648
BsrFI	RCCGGY	3 cut(s)	743, 1124, 1736
BsrI	ACTGG	1 cut(s)	1024
BssAI	RCCGGY	3 cut(s)	743, 1124, 1736
BssECI	CCNNGG	6 cut(s)	234, 873, 1000, 1296, 1366, 1481
BssMI	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
BssNI	GRCGYC	3 cut(s)	263, 567, 890
BssT1I	CCWWGG	2 cut(s)	1366, 1481
Bst4CI	ACNGT	9 cut(s)	398, 551, 758, 835, 868, 1069, 1270, 1374, 1504
Bst6I	CTCTTC	2 cut(s)	347, 893
BstACI	GRCGYC	3 cut(s)	263, 567, 890
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstBAI	YACGTR	2 cut(s)	436, 613
BstC8I	GCNNGC	2 cut(s)	728, 1327
BstDEI	CTNAG	7 cut(s)	34, 465, 479, 517, 686, 751, 1683
BstDSI	CCRYGG	4 cut(s)	234, 873, 1000, 1296
BstEII	GGTNACC	2 cut(s)	739, 1357
BstF5I	GGATG	3 cut(s)	770, 847, 1553
BstFNI	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
BstH2I	RGCGCY	2 cut(s)	389, 893
BstHHI	GCGC	3 cut(s)	388, 892, 1099
BstKTI	GATC	9 cut(s)	348, 451, 499, 648, 1166, 1381, 1407, 1438, 1536
BstMAI	GTCTC	3 cut(s)	398, 410, 783
BstMBI	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
BstMCI	CGRYCG	1 cut(s)	1737
BstMWI	GCNNNNNNNGC	7 cut(s)	80, 239, 836, 881, 884, 1070, 1628
BstNSI	RCATGY	3 cut(s)	491, 625, 1198
BstPI	GGTNACC	2 cut(s)	739, 1357
BstSCI	CCNGG	1 cut(s)	213
BstUI	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
BstV1I	GCAGC	5 cut(s)	210, 251, 871, 1060, 1304
BstV2I	GAAGAC	2 cut(s)	690, 977
BstX2I	RGATCY	2 cut(s)	448, 1435
BstYI	RGATCY	2 cut(s)	448, 1435
BsuI	GTATCC	1 cut(s)	471
BsuRI	GGCC	4 cut(s)	98, 769, 999, 1445
BtgI	CCRYGG	4 cut(s)	234, 873, 1000, 1296
BtrI	CACGTC	2 cut(s)	1431, 1531
BtsCI	GGATG	3 cut(s)	770, 847, 1553
BtsIMutI	CAGTG	2 cut(s)	763, 1017
BtuMI	TCGCGA	1 cut(s)	350
Cac8I	GCNNGC	2 cut(s)	728, 1327
CaiI	CAGNNNCTG	1 cut(s)	1628
CfoI	GCGC	3 cut(s)	388, 892, 1099
Cfr10I	RCCGGY	3 cut(s)	743, 1124, 1736
Cfr13I	GGNCC	1 cut(s)	1127
Cfr42I	CCGCGG	2 cut(s)	876, 1299
CpoI	CGGWCCG	1 cut(s)	1127
CseI	GACGC	4 cut(s)	276, 575, 836, 1719
Csp6I	GTAC	1 cut(s)	608
CspAI	ACCGGT	1 cut(s)	1736
CspI	CGGWCCG	1 cut(s)	1127
CviAII	CATG	6 cut(s)	488, 622, 655, 1195, 1354, 1553
CviQI	GTAC	1 cut(s)	608
DdeI	CTNAG	7 cut(s)	34, 465, 479, 517, 686, 751, 1683
DinI	GGCGCC	1 cut(s)	891
DpnI	GATC	9 cut(s)	347, 450, 498, 647, 1165, 1380, 1406, 1437, 1535
DpnII	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
DraIII	CACNNNGTG	1 cut(s)	374
EaeI	YGGCCR	3 cut(s)	767, 997, 1443
Eam1104I	CTCTTC	2 cut(s)	347, 893
EarI	CTCTTC	2 cut(s)	347, 893
EciI	GGCGGA	3 cut(s)	61, 220, 854
Ecl136II	GAGCTC	2 cut(s)	220, 430
Eco130I	CCWWGG	2 cut(s)	1366, 1481
Eco24I	GRGCYC	2 cut(s)	222, 432
Eco47I	GGWCC	1 cut(s)	1127
Eco53kI	GAGCTC	2 cut(s)	220, 430
Eco72I	CACGTG	2 cut(s)	436, 613
Eco91I	GGTNACC	2 cut(s)	739, 1357
EcoICRI	GAGCTC	2 cut(s)	220, 430
EcoO65I	GGTNACC	2 cut(s)	739, 1357
EcoRI	GAATTC	1 cut(s)	17
EcoT14I	CCWWGG	2 cut(s)	1366, 1481
EcoT38I	GRGCYC	2 cut(s)	222, 432
EgeI	GGCGCC	1 cut(s)	891
EheI	GGCGCC	1 cut(s)	891
ErhI	CCWWGG	2 cut(s)	1366, 1481
Esp3I	CGTCTC	1 cut(s)	410
FaeI	CATG	6 cut(s)	491, 625, 658, 1198, 1357, 1556
FalI	AAGNNNNNCTT	6 cut(s)	276, 308, 1036, 1068, 1200, 1232
FaqI	GGGAC	1 cut(s)	550
FatI	CATG	6 cut(s)	487, 621, 654, 1194, 1353, 1552
FauI	CCCGC	1 cut(s)	1556
FbaI	TGATCA	1 cut(s)	645
FblI	GTMKAC	1 cut(s)	1733
FokI	GGATG	3 cut(s)	777, 854, 1560
FriOI	GRGCYC	2 cut(s)	222, 432
FspBI	CTAG	1 cut(s)	1094
GlaI	GCGC	3 cut(s)	387, 891, 1098
HaeII	RGCGCY	2 cut(s)	389, 893
HaeIII	GGCC	4 cut(s)	98, 769, 999, 1445
HapII	CCGG	7 cut(s)	215, 246, 744, 1125, 1263, 1341, 1737
HgaI	GACGC	4 cut(s)	276, 575, 836, 1719
HhaI	GCGC	3 cut(s)	388, 892, 1099
Hin1I	GRCGYC	3 cut(s)	263, 567, 890
Hin1II	CATG	6 cut(s)	491, 625, 658, 1198, 1357, 1556
Hin6I	GCGC	3 cut(s)	386, 890, 1097
HinP1I	GCGC	3 cut(s)	386, 890, 1097
HincII	GTYRAC	2 cut(s)	813, 1734
HindII	GTYRAC	2 cut(s)	813, 1734
HinfI	GANTC	3 cut(s)	111, 1158, 1384
HpaII	CCGG	7 cut(s)	215, 246, 744, 1125, 1263, 1341, 1737
HphI	GGTGA	3 cut(s)	700, 866, 1516
Hpy166II	GTNNAC	4 cut(s)	610, 813, 1225, 1734
Hpy188III	TCNNGA	7 cut(s)	337, 349, 392, 649, 1208, 1408, 1462
Hpy8I	GTNNAC	4 cut(s)	610, 813, 1225, 1734
HpyAV	CCTTC	6 cut(s)	592, 715, 789, 1480, 1538, 1642
HpyCH4III	ACNGT	9 cut(s)	398, 551, 758, 835, 868, 1069, 1270, 1374, 1504
HpyCH4IV	ACGT	8 cut(s)	263, 419, 435, 612, 1430, 1457, 1466, 1530
HpyCH4V	TGCA	4 cut(s)	198, 1325, 1513, 1631
HpyF10VI	GCNNNNNNNGC	7 cut(s)	80, 239, 836, 881, 884, 1070, 1628
HpyF3I	CTNAG	7 cut(s)	34, 465, 479, 517, 686, 751, 1683
HpySE526I	ACGT	8 cut(s)	263, 419, 435, 612, 1430, 1457, 1466, 1530
Hsp92I	GRCGYC	3 cut(s)	263, 567, 890
Hsp92II	CATG	6 cut(s)	491, 625, 658, 1198, 1357, 1556
HspAI	GCGC	3 cut(s)	386, 890, 1097
KasI	GGCGCC	1 cut(s)	889
Ksp22I	TGATCA	1 cut(s)	645
KspI	CCGCGG	2 cut(s)	876, 1299
Kzo9I	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
LmnI	GCTCC	4 cut(s)	217, 435, 616, 1560
Lsp1109I	GCAGC	5 cut(s)	210, 251, 871, 1060, 1304
LweI	GCATC	1 cut(s)	1147
MaeI	CTAG	1 cut(s)	1094
MaeII	ACGT	8 cut(s)	263, 419, 435, 612, 1430, 1457, 1466, 1530
MalI	GATC	9 cut(s)	347, 450, 498, 647, 1165, 1380, 1406, 1437, 1535
MbiI	CCGCTC	1 cut(s)	1563
MboI	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
MflI	RGATCY	2 cut(s)	448, 1435
MhlI	GDGCHC	3 cut(s)	222, 432, 621
MlsI	TGGCCA	1 cut(s)	769
MluCI	AATT	7 cut(s)	17, 29, 816, 1238, 1336, 1411, 1662
MluNI	TGGCCA	1 cut(s)	769
Mly113I	GGCGCC	1 cut(s)	890
MlyI	GAGTC	1 cut(s)	1167
MmeI	TCCRAC	7 cut(s)	88, 766, 1141, 1413, 1551, 1557, 1561
Mox20I	TGGCCA	1 cut(s)	769
MscI	TGGCCA	1 cut(s)	769
MseI	TTAA	3 cut(s)	294, 819, 1754
MslI	CAYNNNNRTG	1 cut(s)	492
Msp20I	TGGCCA	1 cut(s)	769
MspA1I	CMGCKG	2 cut(s)	875, 1298
MspI	CCGG	7 cut(s)	215, 246, 744, 1125, 1263, 1341, 1737
MspR9I	CCNGG	1 cut(s)	215
MvnI	CGCG	5 cut(s)	350, 373, 875, 1099, 1298
MwoI	GCNNNNNNNGC	7 cut(s)	80, 239, 836, 881, 884, 1070, 1628
NarI	GGCGCC	1 cut(s)	890
NciI	CCSGG	1 cut(s)	215
NdeII	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
NlaIII	CATG	6 cut(s)	491, 625, 658, 1198, 1357, 1556
NlaIV	GGNNCC	3 cut(s)	6, 891, 1267
NmuCI	GTSAC	7 cut(s)	264, 288, 379, 854, 958, 1100, 1253
NruI	TCGCGA	1 cut(s)	350
NspI	RCATGY	3 cut(s)	491, 625, 1198
PciI	ACATGT	2 cut(s)	621, 1194
PcsI	WCGNNNNNNNCGW	2 cut(s)	567, 1463
PfeI	GAWTC	2 cut(s)	111, 1384
PinAI	ACCGGT	1 cut(s)	1736
PleI	GAGTC	1 cut(s)	1166
PluTI	GGCGCC	1 cut(s)	893
PmaCI	CACGTG	2 cut(s)	436, 613
PmlI	CACGTG	2 cut(s)	436, 613
PpsI	GAGTC	1 cut(s)	1166
Ppu21I	YACGTR	2 cut(s)	436, 613
PscI	ACATGT	2 cut(s)	621, 1194
Psp124BI	GAGCTC	2 cut(s)	222, 432
Psp1406I	AACGTT	1 cut(s)	1457
PspCI	CACGTG	2 cut(s)	436, 613
PspEI	GGTNACC	2 cut(s)	739, 1357
PspN4I	GGNNCC	3 cut(s)	6, 891, 1267
PspPI	GGNCC	1 cut(s)	1127
PstNI	CAGNNNCTG	1 cut(s)	1628
PsuI	RGATCY	2 cut(s)	448, 1435
RruI	TCGCGA	1 cut(s)	350
RsaI	GTAC	1 cut(s)	609
RsaNI	GTAC	1 cut(s)	608
RseI	CAYNNNNRTG	1 cut(s)	492
Rsr2I	CGGWCCG	1 cut(s)	1127
RsrII	CGGWCCG	1 cut(s)	1127
SacI	GAGCTC	2 cut(s)	222, 432
SacII	CCGCGG	2 cut(s)	876, 1299
SalI	GTCGAC	1 cut(s)	1732
SaqAI	TTAA	3 cut(s)	294, 819, 1754
Sau3AI	GATC	9 cut(s)	345, 448, 496, 645, 1163, 1378, 1404, 1435, 1533
Sau96I	GGNCC	1 cut(s)	1127
SchI	GAGTC	1 cut(s)	1167
ScrFI	CCNGG	1 cut(s)	215
SduI	GDGCHC	3 cut(s)	222, 432, 621
SfaNI	GCATC	1 cut(s)	1147
SfoI	GGCGCC	1 cut(s)	891
Sfr303I	CCGCGG	2 cut(s)	876, 1299
SgrBI	CCGCGG	2 cut(s)	876, 1299
SinI	GGWCC	1 cut(s)	1127
SmiMI	CAYNNNNRTG	1 cut(s)	492
SmlI	CTYRAG	1 cut(s)	731
SmoI	CTYRAG	1 cut(s)	731
Sse9I	AATT	7 cut(s)	17, 29, 816, 1238, 1336, 1411, 1662
SspDI	GGCGCC	1 cut(s)	889
SspI	AATATT	1 cut(s)	1493
SspMI	CTAG	1 cut(s)	1094
SstI	GAGCTC	2 cut(s)	222, 432
StyD4I	CCNGG	1 cut(s)	213
StyI	CCWWGG	2 cut(s)	1366, 1481
TaaI	ACNGT	9 cut(s)	398, 551, 758, 835, 868, 1069, 1270, 1374, 1504
TaiI	ACGT	8 cut(s)	266, 422, 438, 615, 1433, 1460, 1469, 1533
TaqI	TCGA	7 cut(s)	167, 282, 411, 1038, 1133, 1272, 1733
TasI	AATT	7 cut(s)	17, 29, 816, 1238, 1336, 1411, 1662
TauI	GCSGC	6 cut(s)	77, 119, 875, 878, 890, 1298
TfiI	GAWTC	2 cut(s)	111, 1384
Tru1I	TTAA	3 cut(s)	294, 819, 1754
Tru9I	TTAA	3 cut(s)	294, 819, 1754
TscAI	CASTG	2 cut(s)	763, 1024
TseFI	GTSAC	7 cut(s)	264, 288, 379, 854, 958, 1100, 1253
TseI	GCWGC	5 cut(s)	198, 239, 884, 1073, 1292
Tsp45I	GTSAC	7 cut(s)	264, 288, 379, 854, 958, 1100, 1253
TspDTI	ATGAA	3 cut(s)	10, 443, 1267
TspGWI	ACGGA	4 cut(s)	772, 861, 1119, 1513
TspRI	CASTG	2 cut(s)	763, 1024
VpaK11BI	GGWCC	1 cut(s)	1127
XapI	RAATTY	3 cut(s)	17, 29, 1336
XceI	RCATGY	3 cut(s)	491, 625, 1198
XmiI	GTMKAC	1 cut(s)	1733
XspI	CTAG	1 cut(s)	1094
ZraI	GACGTC	1 cut(s)	264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.