Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012707

Physical Location & Seq

Genomic Coordinates Reverse (-)

14640195 .. 14645899

Gene Structure 5705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1154500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCCAAAATCAAAGAGTTTTTGACCAAAATGTCCGAATCGGGCAAACAAATATCCACAAACAAGAAACACAAGAAGCTTTTCTTATCCCTTTTCGCAACCATCCTACTAGTCGGCGCCGTCGTCGGCATTGTCACCGGAGTCAAGTCCTCCAAAAACAACTCCGACGACGAAACCAACGAGGCCTCCCACGCCATAGTCAAATCCTCATGCAGCTCCACCCTCTACCCTGAACTGTGCTTTTCCACTCTTGCACTTCACCCCGAAGCCGCCAAAAAGGTTTCGAGCCAGAAGGACGCGATAGAATTGTCATTGAACATCACGACCAAGGCGGTTCAGCACAACTTCTTCACTGTCGAGAAGCTGCTGAAGTCCAGGAAGAATAAGCTCACTAAGCGTGAGAAGGGCGCTCTTCACGACTGTTTGGAGACCATTGATGAAACTCTCGACGAGCTTCACGATGCTGTGGAGGACCTCCATGAGTACCCTAATAAGAAGACTTTGACTCAGCATGCGGATGACCTAAAAACTTTGATCAGCTCCGCCATTACCAACCAAGAAACTTGCCTTGATGGGTTCTCGCACGATGATGCTGATAAAAAAGTTAGGAAAGTTTTTTTGGCCGGTCAGGTGCACGTAGAAAAATTGTGCAGTAATGCGCTGGCCATGATCAAGAACATGACCGATACCGACATTGCAAACGAGATGAAAACGAATGTGAACCGAAAGCTGAAGGAGGATGTGGTGGACGAGCATGGGTGGCCGGAGTGGCTGTCGGTGGCAGACAGGCGACTGCTGCAGTCGTCATCTGTCACACCAAATGTGGTGGTGGCAGCAGACGGGAGTGGGAACTACAAGACGGTGTCGGCTGCGGTGGCTGCCGCCCCGGAGAAGAGCAGTAAAAGATATGTGATTAGAATAAAGGCGGGGGTTTATAGAGAAAATGTGGACGTGCCGAAGAAAAAGACCAACATTATGTTTATGGGAGATGGGAGAAAAAACACTATCATCACTGCTAGTAAAAATGTGGTCGATGGCAGCACAACTTTCAACTCGGCCACAGTTGCTGCGGTTGGTGAGAAGTTTCTAGCCCGAGACATAACGTTCCAAAACACAGCAGGTCCATCAAAGCACCAAGCTGTTGCTCTAAGAGTAGGGTCCGATCTCTCAGCATTCTACCGGTGCGACATTCTTGCGTACCAAGACTCGCTCTATGTGCACTCAAACCGCCAGTTTTTCGAAGGCTGCTTGGTAGCCGGAACAGTTGACTTCATCTTCGGCAATGCTGCTGTCGTGTTACAAAACTGTGACATCCATGCAAGAAAACCTAATTCCGGCCAGAAGAACATGCTCACCGCACAAGGCAGAACTGACCCGAATCAAAACACCGGCATTGTGATCCAAAAATCGAGAATCGGCGCCACATCCGACTTGCAAGCCTCAAAGGGCAGCTTTAAAACGTTCTTGGGGAGGCCATGGAAGGAGTATTCAAGGACTGTGATCATGCAGTCAAGTATAACGGATATCATTGACCCTGTTGGGTGGCATGAGTGGTCTGGCACTTTTGCCCTCAACACTTTGTTTTATGGAGAGTATGCTAACACTGGGGCAGGCGCTGGGACTTCAAAAAGGGTGACGTGGAAGGGGTTTAAGGTAATTACAAGTACGACTGAGGCTCAGGCGTATACGCCCGGCAGATTCATTGCTGGTGGTAGTTGGTTGAGTTCTACTGGTTTTCCATTTGCTCTTGGGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

586

Amino Acids

64.0

Weight (kDa)

9.07

Isoelectric Point (pI)

25.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	66 - 223	1.9e-36	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	274 - 570	1.5e-146	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012707)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53830 AT3G14310
fragaria_vesca	FvH4_6g35830
malus_domestica	MD09G1172600.v1.1 MD17G1154500.v1.1
prunus_persica	Prupe.3G031000_v2.0.a1
pyrus_communis	pycom09g09010 pycom09g09020
rosa_chinensis	RchiOBHm_Chr2g0147881
rosa_laevigata	RLG00000020287
rosa_multiflora	Rmu_sc0000308.1_g000057
rosa_roxburghii	Rroxscaffold_2G00099240
rosa_rugosa	Rorug02G0402600
rosa_samantha	Rh2AG459500 Rh2BG472900 Rh2CG446900 Rh2DG481800
rosa_wichuraiana	Rw2G037590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	31
Acc36I	ACCTGC	1 cut(s)	1109
AccB1I	GGYRCC	2 cut(s)	116, 1418
AccI	GTMKAC	1 cut(s)	1685
AccII	CGCG	1 cut(s)	299
AclI	AACGTT	2 cut(s)	1103, 1460
AclWI	GGATC	1 cut(s)	1393
AcoI	YGGCCR	6 cut(s)	3, 621, 663, 761, 1056, 1336
AcuI	CTGAAG	2 cut(s)	389, 752
AcyI	GRCGYC	2 cut(s)	117, 1419
AfaI	GTAC	3 cut(s)	485, 1199, 1666
AfiI	CCNNNNNNNGG	1 cut(s)	1334
AgeI	ACCGGT	1 cut(s)	1179
AgsI	TTSAA	4 cut(s)	316, 1051, 1491, 1626
AhlI	ACTAGT	1 cut(s)	109
AjiI	CACGTC	2 cut(s)	952, 1638
AjnI	CCWGG	1 cut(s)	374
AjuI	GAANNNNNNNTTGG	2 cut(s)	602, 634
AluBI	AGCT	9 cut(s)	79, 216, 364, 388, 454, 540, 730, 1139, 1452
AluI	AGCT	9 cut(s)	79, 216, 364, 388, 454, 540, 730, 1139, 1452
Alw21I	GWGCWC	2 cut(s)	636, 1221
Alw26I	GTCTC	2 cut(s)	422, 1089
Alw44I	GTGCAC	2 cut(s)	632, 1217
AlwI	GGATC	1 cut(s)	1393
AlwNI	CAGNNNCTG	2 cut(s)	1067, 1616
Ama87I	CYCGRG	1 cut(s)	1092
AoxI	GGCC	8 cut(s)	3, 183, 621, 663, 761, 1056, 1336, 1472
ApaLI	GTGCAC	2 cut(s)	632, 1217
ArsI	GACNNNNNNTTYG	2 cut(s)	1259, 1291
AsiGI	ACCGGT	1 cut(s)	1179
Asp700I	GAANNNNTTC	1 cut(s)	80
AspLEI	GCGC	5 cut(s)	119, 410, 661, 1421, 1616
AspS9I	GGNCC	3 cut(s)	472, 1121, 1158
AsuC2I	CCSGG	2 cut(s)	887, 1692
AsuHPI	GGTGA	5 cut(s)	127, 251, 1088, 1345, 1645
AsuII	TTCGAA	1 cut(s)	1239
AvaI	CYCGRG	1 cut(s)	1092
AvaII	GGWCC	3 cut(s)	472, 1121, 1158
BaeGI	GKGCMC	2 cut(s)	636, 1221
BalI	TGGCCA	2 cut(s)	5, 665
BanI	GGYRCC	2 cut(s)	116, 1418
BbsI	GAAGAC	1 cut(s)	503
Bbv12I	GWGCWC	2 cut(s)	636, 1221
BccI	CCATC	5 cut(s)	110, 566, 983, 1028, 1132
BceAI	ACGGC	1 cut(s)	104
BcgI	CGANNNNNNTGC	2 cut(s)	1175, 1209
BciT130I	CCWGG	1 cut(s)	376
BclI	TGATCA	3 cut(s)	534, 669, 1500
BcnI	CCSGG	2 cut(s)	887, 1692
BcoDI	GTCTC	2 cut(s)	422, 1089
BcuI	ACTAGT	1 cut(s)	109
BfaI	CTAG	3 cut(s)	110, 1017, 1088
BfmI	CTRYAG	1 cut(s)	797
BfoI	RGCGCY	4 cut(s)	120, 411, 1422, 1617
BfuAI	ACCTGC	1 cut(s)	1109
BglI	GCCNNNNNGGC	1 cut(s)	769
Bme1390I	CCNGG	3 cut(s)	376, 887, 1692
Bme18I	GGWCC	3 cut(s)	472, 1121, 1158
BmeT110I	CYCGRG	1 cut(s)	1092
BmgBI	CACGTC	2 cut(s)	952, 1638
BmgT120I	GGNCC	3 cut(s)	472, 1121, 1158
BmiI	GGNNCC	3 cut(s)	118, 1159, 1420
BmrFI	CCNGG	3 cut(s)	376, 887, 1692
BmrI	ACTGGG	1 cut(s)	1614
BmsI	GCATC	2 cut(s)	451, 580
BmuI	ACTGGG	1 cut(s)	1614
BpiI	GAAGAC	1 cut(s)	503
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu14I	TTCGAA	1 cut(s)	1239
BpuMI	CCSGG	2 cut(s)	887, 1692
BsaAI	YACGTR	1 cut(s)	637
BsaHI	GRCGYC	2 cut(s)	117, 1419
BsaI	GGTCTC	1 cut(s)	422
BsaJI	CCNNGG	3 cut(s)	327, 885, 1475
BsaWI	WCCGGW	2 cut(s)	137, 1179
BsaXI	ACNNNNNCTCC	4 cut(s)	170, 200, 758, 788
Bsc4I	CCNNNNNNNGG	1 cut(s)	1334
Bse118I	RCCGGY	3 cut(s)	623, 1179, 1388
Bse1I	ACTGG	3 cut(s)	1231, 1609, 1735
Bse3DI	GCAATG	3 cut(s)	693, 1288, 1701
BseBI	CCWGG	1 cut(s)	376
BseDI	CCNNGG	3 cut(s)	327, 885, 1475
BseGI	GGATG	5 cut(s)	102, 523, 745, 1311, 1424
BseLI	CCNNNNNNNGG	1 cut(s)	1334
BseMI	GCAATG	3 cut(s)	693, 1288, 1701
BseMII	CTCAG	4 cut(s)	521, 1182, 1662, 1691
BseNI	ACTGG	3 cut(s)	1231, 1609, 1735
BseSI	GKGCMC	2 cut(s)	636, 1221
BseYI	CCCAGC	1 cut(s)	1616
BsgI	GTGCAG	1 cut(s)	670
Bsh1236I	CGCG	1 cut(s)	299
BshFI	GGCC	8 cut(s)	5, 185, 623, 665, 763, 1058, 1338, 1474
BshNI	GGYRCC	2 cut(s)	116, 1418
BshTI	ACCGGT	1 cut(s)	1179
BsiHKAI	GWGCWC	2 cut(s)	636, 1221
BsiHKCI	CYCGRG	1 cut(s)	1092
BsiSI	CCGG	9 cut(s)	138, 624, 764, 887, 1180, 1257, 1335, 1389, 1692
BslFI	GGGAC	1 cut(s)	1633
BslI	CCNNNNNNNGG	1 cut(s)	1334
BsmAI	GTCTC	2 cut(s)	422, 1089
BsmFI	GGGAC	1 cut(s)	1633
BsmI	GAATGC	1 cut(s)	1172
BsnI	GGCC	8 cut(s)	5, 185, 623, 665, 763, 1058, 1338, 1474
Bso31I	GGTCTC	1 cut(s)	422
BsoBI	CYCGRG	1 cut(s)	1092
Bsp119I	TTCGAA	1 cut(s)	1239
Bsp1286I	GDGCHC	2 cut(s)	636, 1221
Bsp143I	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
Bsp19I	CCATGG	1 cut(s)	1475
BspANI	GGCC	8 cut(s)	5, 185, 623, 665, 763, 1058, 1338, 1474
BspCNI	CTCAG	4 cut(s)	520, 1181, 1663, 1690
BspFNI	CGCG	1 cut(s)	299
BspLI	GGNNCC	3 cut(s)	118, 1159, 1420
BspMAI	CTGCAG	1 cut(s)	801
BspMI	ACCTGC	1 cut(s)	1109
BspPI	GGATC	1 cut(s)	1393
BspQI	GCTCTTC	2 cut(s)	417, 887
BspT104I	TTCGAA	1 cut(s)	1239
BspT107I	GGYRCC	2 cut(s)	116, 1418
BspTNI	GGTCTC	1 cut(s)	422
BsrDI	GCAATG	3 cut(s)	693, 1288, 1701
BsrFI	RCCGGY	3 cut(s)	623, 1179, 1388
BsrI	ACTGG	3 cut(s)	1231, 1609, 1735
BssAI	RCCGGY	3 cut(s)	623, 1179, 1388
BssECI	CCNNGG	3 cut(s)	327, 885, 1475
BssMI	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
BssNAI	GTATAC	1 cut(s)	1686
BssNI	GRCGYC	2 cut(s)	117, 1419
BssT1I	CCWWGG	2 cut(s)	327, 1475
Bst1107I	GTATAC	1 cut(s)	1686
Bst2UI	CCWGG	1 cut(s)	376
Bst4CI	ACNGT	8 cut(s)	237, 355, 422, 862, 1063, 1264, 1307, 1498
Bst6I	CTCTTC	2 cut(s)	417, 887
BstACI	GRCGYC	2 cut(s)	117, 1419
BstBAI	YACGTR	1 cut(s)	637
BstBI	TTCGAA	1 cut(s)	1239
BstC8I	GCNNGC	4 cut(s)	513, 663, 1437, 1612
BstDEI	CTNAG	6 cut(s)	393, 507, 1148, 1168, 1671, 1677
BstDSI	CCRYGG	1 cut(s)	1475
BstF5I	GGATG	5 cut(s)	102, 523, 745, 1311, 1424
BstFNI	CGCG	1 cut(s)	299
BstH2I	RGCGCY	4 cut(s)	120, 411, 1422, 1617
BstHHI	GCGC	5 cut(s)	119, 410, 661, 1421, 1616
BstKTI	GATC	5 cut(s)	537, 672, 1165, 1401, 1503
BstMAI	GTCTC	2 cut(s)	422, 1089
BstMBI	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 875, 878, 1064, 1216
BstNI	CCWGG	1 cut(s)	376
BstNSI	RCATGY	2 cut(s)	515, 1351
BstSCI	CCNGG	3 cut(s)	374, 885, 1690
BstSFI	CTRYAG	1 cut(s)	797
BstSLI	GKGCMC	2 cut(s)	636, 1221
BstUI	CGCG	1 cut(s)	299
BstV2I	GAAGAC	1 cut(s)	503
BstZ17I	GTATAC	1 cut(s)	1686
BsuRI	GGCC	8 cut(s)	5, 185, 623, 665, 763, 1058, 1338, 1474
BtgI	CCRYGG	1 cut(s)	1475
BtrI	CACGTC	2 cut(s)	952, 1638
BtsCI	GGATG	5 cut(s)	102, 523, 745, 1311, 1424
BtsI	GCAGTG	1 cut(s)	1011
BtsIMutI	CAGTG	3 cut(s)	351, 1011, 1602
BveI	ACCTGC	1 cut(s)	1109
Cac8I	GCNNGC	4 cut(s)	513, 663, 1437, 1612
CaiI	CAGNNNCTG	2 cut(s)	1067, 1616
CfoI	GCGC	5 cut(s)	119, 410, 661, 1421, 1616
Cfr10I	RCCGGY	3 cut(s)	623, 1179, 1388
Cfr13I	GGNCC	3 cut(s)	472, 1121, 1158
CseI	GACGC	1 cut(s)	305
Csp6I	GTAC	3 cut(s)	484, 1198, 1665
CspAI	ACCGGT	1 cut(s)	1179
CspCI	CAANNNNNGTGG	2 cut(s)	807, 842
CviQI	GTAC	3 cut(s)	484, 1198, 1665
DdeI	CTNAG	6 cut(s)	393, 507, 1148, 1168, 1671, 1677
DinI	GGCGCC	2 cut(s)	118, 1420
DpnI	GATC	5 cut(s)	536, 671, 1164, 1400, 1502
DpnII	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
DraI	TTTAAA	1 cut(s)	1456
DrdI	GACNNNNNNGTC	1 cut(s)	31
DseDI	GACNNNNNNGTC	1 cut(s)	31
EaeI	YGGCCR	6 cut(s)	3, 621, 663, 761, 1056, 1336
Eam1104I	CTCTTC	2 cut(s)	417, 887
EarI	CTCTTC	2 cut(s)	417, 887
EciI	GGCGGA	1 cut(s)	532
Eco130I	CCWWGG	2 cut(s)	327, 1475
Eco147I	AGGCCT	1 cut(s)	185
Eco31I	GGTCTC	1 cut(s)	422
Eco32I	GATATC	1 cut(s)	1525
Eco47I	GGWCC	3 cut(s)	472, 1121, 1158
Eco57I	CTGAAG	2 cut(s)	389, 752
Eco88I	CYCGRG	1 cut(s)	1092
EcoO109I	RGGNCCY	1 cut(s)	472
EcoRII	CCWGG	1 cut(s)	374
EcoRV	GATATC	1 cut(s)	1525
EcoT14I	CCWWGG	2 cut(s)	327, 1475
EgeI	GGCGCC	2 cut(s)	118, 1420
EheI	GGCGCC	2 cut(s)	118, 1420
ErhI	CCWWGG	2 cut(s)	327, 1475
FalI	AAGNNNNNCTT	4 cut(s)	68, 100, 1436, 1468
FaqI	GGGAC	1 cut(s)	1633
FauI	CCCGC	1 cut(s)	919
FbaI	TGATCA	3 cut(s)	534, 669, 1500
FblI	GTMKAC	1 cut(s)	1685
FokI	GGATG	5 cut(s)	89, 530, 752, 1298, 1411
FspBI	CTAG	3 cut(s)	110, 1017, 1088
GlaI	GCGC	5 cut(s)	118, 409, 660, 1420, 1615
GsaI	CCCAGC	1 cut(s)	1620
HaeII	RGCGCY	4 cut(s)	120, 411, 1422, 1617
HaeIII	GGCC	8 cut(s)	5, 185, 623, 665, 763, 1058, 1338, 1474
HapII	CCGG	9 cut(s)	138, 624, 764, 887, 1180, 1257, 1335, 1389, 1692
HgaI	GACGC	1 cut(s)	305
HhaI	GCGC	5 cut(s)	119, 410, 661, 1421, 1616
Hin1I	GRCGYC	2 cut(s)	117, 1419
Hin6I	GCGC	5 cut(s)	117, 408, 659, 1419, 1614
HinP1I	GCGC	5 cut(s)	117, 408, 659, 1419, 1614
HincII	GTYRAC	1 cut(s)	1267
HindII	GTYRAC	1 cut(s)	1267
HindIII	AAGCTT	1 cut(s)	77
HinfI	GANTC	7 cut(s)	38, 141, 505, 1205, 1378, 1413, 1698
HpaII	CCGG	9 cut(s)	138, 624, 764, 887, 1180, 1257, 1335, 1389, 1692
HphI	GGTGA	5 cut(s)	127, 251, 1088, 1345, 1645
Hpy166II	GTNNAC	7 cut(s)	634, 721, 748, 949, 1219, 1267, 1686
Hpy188I	TCNGA	4 cut(s)	37, 166, 1162, 1429
Hpy188III	TCNNGA	7 cut(s)	322, 358, 416, 446, 458, 673, 1410
Hpy8I	GTNNAC	7 cut(s)	634, 721, 748, 949, 1219, 1267, 1686
Hpy99I	CGWCG	5 cut(s)	125, 128, 170, 173, 452
HpyAV	CCTTC	6 cut(s)	286, 397, 727, 1235, 1474, 1636
HpyCH4III	ACNGT	8 cut(s)	237, 355, 422, 862, 1063, 1264, 1307, 1498
HpyCH4IV	ACGT	5 cut(s)	636, 951, 1103, 1460, 1637
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 875, 878, 1064, 1216
HpyF3I	CTNAG	6 cut(s)	393, 507, 1148, 1168, 1671, 1677
HpySE526I	ACGT	5 cut(s)	636, 951, 1103, 1460, 1637
Hsp92I	GRCGYC	2 cut(s)	117, 1419
HspAI	GCGC	5 cut(s)	117, 408, 659, 1419, 1614
KasI	GGCGCC	2 cut(s)	116, 1418
Ksp22I	TGATCA	3 cut(s)	534, 669, 1500
Kzo9I	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
LguI	GCTCTTC	2 cut(s)	417, 887
LmnI	GCTCC	2 cut(s)	221, 545
LweI	GCATC	2 cut(s)	451, 580
MaeI	CTAG	3 cut(s)	110, 1017, 1088
MaeII	ACGT	5 cut(s)	636, 951, 1103, 1460, 1637
MaeIII	GTNAC	5 cut(s)	133, 811, 1296, 1307, 1633
MalI	GATC	5 cut(s)	536, 671, 1164, 1400, 1502
MboI	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
MboII	GAAGA	8 cut(s)	340, 391, 404, 508, 904, 970, 1267, 1354
MhlI	GDGCHC	2 cut(s)	636, 1221
MlsI	TGGCCA	2 cut(s)	5, 665
MluCI	AATT	4 cut(s)	305, 644, 1330, 1656
MluNI	TGGCCA	2 cut(s)	5, 665
Mly113I	GGCGCC	2 cut(s)	117, 1419
MlyI	GAGTC	3 cut(s)	150, 499, 1199
MmeI	TCCRAC	2 cut(s)	189, 1452
Mox20I	TGGCCA	2 cut(s)	5, 665
MroXI	GAANNNNTTC	1 cut(s)	80
MscI	TGGCCA	2 cut(s)	5, 665
MseI	TTAA	2 cut(s)	1455, 1650
MslI	CAYNNNNRTG	2 cut(s)	516, 588
Msp20I	TGGCCA	2 cut(s)	5, 665
MspI	CCGG	9 cut(s)	138, 624, 764, 887, 1180, 1257, 1335, 1389, 1692
MspR9I	CCNGG	3 cut(s)	376, 887, 1692
Mva1269I	GAATGC	1 cut(s)	1172
MvaI	CCWGG	1 cut(s)	376
MvnI	CGCG	1 cut(s)	299
MwoI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 875, 878, 1064, 1216
NarI	GGCGCC	2 cut(s)	117, 1419
NciI	CCSGG	2 cut(s)	887, 1692
NcoI	CCATGG	1 cut(s)	1475
NdeII	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
NlaIV	GGNNCC	3 cut(s)	118, 1159, 1420
NmeAIII	GCCGAG	1 cut(s)	1034
NmuCI	GTSAC	4 cut(s)	133, 811, 1307, 1633
NspI	RCATGY	2 cut(s)	515, 1351
NspV	TTCGAA	1 cut(s)	1239
PaeI	GCATGC	1 cut(s)	515
PceI	AGGCCT	1 cut(s)	185
PciSI	GCTCTTC	2 cut(s)	417, 887
PcsI	WCGNNNNNNNCGW	3 cut(s)	120, 177, 456
PctI	GAATGC	1 cut(s)	1172
PdmI	GAANNNNTTC	1 cut(s)	80
PfeI	GAWTC	4 cut(s)	38, 1378, 1413, 1698
PflFI	GACNNNGTC	1 cut(s)	862
PfoI	TCCNGGA	1 cut(s)	374
PinAI	ACCGGT	1 cut(s)	1179
PleI	GAGTC	3 cut(s)	149, 499, 1199
PluTI	GGCGCC	2 cut(s)	120, 1422
PpsI	GAGTC	3 cut(s)	149, 499, 1199
Ppu21I	YACGTR	1 cut(s)	637
PpuMI	RGGWCCY	1 cut(s)	472
Psp1406I	AACGTT	2 cut(s)	1103, 1460
Psp5II	RGGWCCY	1 cut(s)	472
Psp6I	CCWGG	1 cut(s)	374
PspFI	CCCAGC	1 cut(s)	1616
PspGI	CCWGG	1 cut(s)	374
PspN4I	GGNNCC	3 cut(s)	118, 1159, 1420
PspPI	GGNCC	3 cut(s)	472, 1121, 1158
PspPPI	RGGWCCY	1 cut(s)	472
PstI	CTGCAG	1 cut(s)	801
PstNI	CAGNNNCTG	2 cut(s)	1067, 1616
PsyI	GACNNNGTC	1 cut(s)	862
RsaI	GTAC	3 cut(s)	485, 1199, 1666
RsaNI	GTAC	3 cut(s)	484, 1198, 1665
RseI	CAYNNNNRTG	2 cut(s)	516, 588
SapI	GCTCTTC	2 cut(s)	417, 887
SaqAI	TTAA	2 cut(s)	1455, 1650
Sau3AI	GATC	5 cut(s)	534, 669, 1162, 1398, 1500
Sau96I	GGNCC	3 cut(s)	472, 1121, 1158
SchI	GAGTC	3 cut(s)	150, 499, 1199
ScrFI	CCNGG	3 cut(s)	376, 887, 1692
SduI	GDGCHC	2 cut(s)	636, 1221
SfaNI	GCATC	2 cut(s)	451, 580
SfcI	CTRYAG	1 cut(s)	797
SfoI	GGCGCC	2 cut(s)	118, 1420
SfuI	TTCGAA	1 cut(s)	1239
SinI	GGWCC	3 cut(s)	472, 1121, 1158
SmiMI	CAYNNNNRTG	2 cut(s)	516, 588
SpeI	ACTAGT	1 cut(s)	109
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	4 cut(s)	305, 644, 1330, 1656
SseBI	AGGCCT	1 cut(s)	185
SspDI	GGCGCC	2 cut(s)	116, 1418
SspMI	CTAG	3 cut(s)	110, 1017, 1088
StuI	AGGCCT	1 cut(s)	185
StyD4I	CCNGG	3 cut(s)	374, 885, 1690
StyI	CCWWGG	2 cut(s)	327, 1475
TaaI	ACNGT	8 cut(s)	237, 355, 422, 862, 1063, 1264, 1307, 1498
TaiI	ACGT	5 cut(s)	639, 954, 1106, 1463, 1640
TaqI	TCGA	6 cut(s)	284, 357, 447, 1032, 1239, 1409
TaqII	GACCGA	1 cut(s)	698
TasI	AATT	4 cut(s)	305, 644, 1330, 1656
TauI	GCSGC	2 cut(s)	272, 884
TfiI	GAWTC	4 cut(s)	38, 1378, 1413, 1698
Tru1I	TTAA	2 cut(s)	1455, 1650
Tru9I	TTAA	2 cut(s)	1455, 1650
TscAI	CASTG	3 cut(s)	358, 1018, 1609
TseFI	GTSAC	4 cut(s)	133, 811, 1307, 1633
Tsp45I	GTSAC	4 cut(s)	133, 811, 1307, 1633
TspDTI	ATGAA	4 cut(s)	453, 722, 1261, 1690
TspGWI	ACGGA	1 cut(s)	1535
TspRI	CASTG	3 cut(s)	358, 1018, 1609
Tth111I	GACNNNGTC	1 cut(s)	862
VneI	GTGCAC	2 cut(s)	632, 1217
VpaK11BI	GGWCC	3 cut(s)	472, 1121, 1158
XceI	RCATGY	2 cut(s)	515, 1351
XmiI	GTMKAC	1 cut(s)	1685
XmnI	GAANNNNTTC	1 cut(s)	80
XspI	CTAG	3 cut(s)	110, 1017, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.