Rh2BG472900

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012707
Physical Location & Seq
Reverse (-)
66744523 .. 66750069
5547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG472900.1

Sequence Viewer

Length: 1323 bp
ATGGTTGGTGTTGAACATGGTAGAATAGTAGAAAGAATTATATATAGGGCCAGTGAGATTAATTCAAACTACAAAAGACTTAATTCACTTCCTTCAGTAGTTTTAATTATGTCTACACTGTTAATATATTTGTTTCTATATTTTGGACCGACTTTTATAGTTTTATTTAAATTACCGTCTTTTTCTGTTCAGAAATATGTAGAAAAGTTGTGTAGCAATGCACTGGCCATGATTAAAAACATGACTGATAACGACATTGCAAACGAGATGAAAGCCATGTCGTCGACGACTAACCGAAAGCTAAAGGAGGAACATTCAAACGGATGGCCGGAGTGGCTGTCTGCCGCAGATAGACATCTCTTGCAATCCTCTACTGTCACACCGAATGTGATCGTGGCAGCAGACGGAACCGGAGATTACAAGACGGTTTCGGAAGCCGTCGCGGCTGCACCATCGAAGAGTAGCACCAGATACGTGATCAGAATCAAGGCCGGTGTGTATAGAGAGAATGTGGATGTGCCAAAGGCCAAGACGAACATAATGTTCTTGGGCGATGGAAGGAAAACCACAATTATTACCGCCAGTAGAAATGTGGTCGATGGGGACACGACCTTCAATTCTGCCACAGTCGCGGCGGTTGGTGCGGGATTCTTAGCAAAAGAAGTCACCTTCCAGAACTCAGCCGGTCCCTCAAAACACCAAGCAGTTGCTCTTCGTGTTGGTTCCGATCTCTCGGCCTTCTACCGCTGCGATGTTATTGCGTACCAAGACTCCCTCTACGTCCACTCGAACCGTCAGTTCTTCGAGGGCTGCTTTGTGGCGGGTACTGTCGATTTCATCTTCGGCAATGCTGCAGTAGTGTTACAAAACTGCGATATCCATGCCCGAAAGCCCAATTCGGGCCAGAAGAACATGCTCACAGCCCAAGGGAGAACCGACCCCAACCAGAACACCGGCATTGTAATCCAAAAATCAAGGATTGGTGCCACTTCTGACTTGGAAGCCGAGAAGGGTAGCTTCAAAACATATCTTGGGAGGCCATGGAAGGAGTACTCGAGGACTGTGATTATGCAATCAGTCATTAGTGATATCATAGACCCTGCTGGGTGGTACGAGTGGAGTGGGACTTTTGCCCTAGACACATTGTTCTATGCCGAATATGCTAACACTGGAGCTGGTGCTGCAACTACGAACAGGGTGACATGGAAGGGGTACAAGGTGATAACAAGTGCCACTGAGGCTCAGGCTTTCACACCAGGGAATTTTATTGCTGGTGGAAGTTGGTTGAGTGCTACTGGTTTTCCATTTACTCTAGGTCTGTAG

Protein Analysis

440

Amino Acids

48.09

Weight (kDa)

9.03

Isoelectric Point (pI)

20.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 129 - 425 2.8e-144 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53830 AT3G14310
fragaria_vesca FvH4_6g35830
malus_domestica MD09G1172600.v1.1 MD17G1154500.v1.1
prunus_persica Prupe.3G031000_v2.0.a1
pyrus_communis pycom09g09010 pycom09g09020
rosa_chinensis RchiOBHm_Chr2g0147881
rosa_laevigata RLG00000020287
rosa_multiflora Rmu_sc0000308.1_g000057
rosa_roxburghii Rroxscaffold_2G00099240
rosa_rugosa Rorug02G0402600
rosa_samantha Rh2AG459500 Rh2BG472900 Rh2CG446900 Rh2DG481800
rosa_wichuraiana Rw2G037590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 983
AccI GTMKAC 2 cut(s) 113, 284
AccII CGCG 2 cut(s) 443, 632
AciI CCGC 8 cut(s) 345, 443, 579, 632, 635, 644, 745, 821
AcoI YGGCCR 2 cut(s) 225, 326
AcsI RAATTY 1 cut(s) 1261
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 5 cut(s) 764, 826, 1052, 1112, 1214
AfiI CCNNNNNNNGG 1 cut(s) 899
AgsI TTSAA 5 cut(s) 14, 66, 318, 616, 1021
AjnI CCWGG 1 cut(s) 1255
AluBI AGCT 3 cut(s) 301, 1017, 1175
AluI AGCT 3 cut(s) 301, 1017, 1175
Ama87I CYCGRG 1 cut(s) 1054
AoxI GGCC 8 cut(s) 48, 225, 326, 489, 525, 735, 901, 1037
ApeKI GCWGC 6 cut(s) 398, 446, 747, 810, 851, 1181
ApoI RAATTY 1 cut(s) 1261
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 4 cut(s) 48, 146, 686, 901
AsuHPI GGTGA 3 cut(s) 658, 1210, 1231
AvaI CYCGRG 1 cut(s) 1054
AvaII GGWCC 2 cut(s) 146, 686
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 1 cut(s) 983
BbvI GCAGC 6 cut(s) 410, 433, 734, 797, 838, 1168
BccI CCATC 4 cut(s) 318, 460, 548, 593
BceAI ACGGC 1 cut(s) 422
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 863, 897
BciT130I CCWGG 1 cut(s) 1257
BclI TGATCA 1 cut(s) 477
BfaI CTAG 2 cut(s) 1136, 1313
BfmI CTRYAG 2 cut(s) 852, 1319
BglI GCCNNNNNGGC 3 cut(s) 334, 443, 1238
BisI GCNGC 9 cut(s) 345, 399, 444, 447, 633, 748, 811, 852, 1182
BlsI GCNGC 9 cut(s) 346, 400, 445, 448, 634, 749, 812, 853, 1183
BmcAI AGTACT 1 cut(s) 1052
Bme1390I CCNGG 1 cut(s) 1257
Bme18I GGWCC 2 cut(s) 146, 686
BmeT110I CYCGRG 1 cut(s) 1054
BmgT120I GGNCC 4 cut(s) 48, 146, 686, 901
BmiI GGNNCC 4 cut(s) 409, 688, 724, 985
BmrFI CCNGG 1 cut(s) 1257
BpmI CTGGAG 1 cut(s) 1191
Bpu10I CCTNAGC 1 cut(s) 1242
BsaAI YACGTR 1 cut(s) 475
BsaBI GATNNNNATC 2 cut(s) 354, 482
BsaJI CCNNGG 3 cut(s) 925, 1040, 1256
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 755, 785
Bsc4I CCNNNNNNNGG 1 cut(s) 899
Bse118I RCCGGY 3 cut(s) 491, 683, 953
Bse1I ACTGG 5 cut(s) 51, 228, 582, 1174, 1300
Bse3DI GCAATG 3 cut(s) 223, 255, 853
Bse8I GATNNNNATC 2 cut(s) 354, 482
BseBI CCWGG 1 cut(s) 1257
BseDI CCNNGG 3 cut(s) 925, 1040, 1256
BseGI GGATG 2 cut(s) 329, 520
BseJI GATNNNNATC 2 cut(s) 354, 482
BseLI CCNNNNNNNGG 1 cut(s) 899
BseMI GCAATG 3 cut(s) 223, 255, 853
BseMII CTCAG 3 cut(s) 693, 1227, 1256
BseNI ACTGG 5 cut(s) 51, 228, 582, 1174, 1300
BseXI GCAGC 6 cut(s) 410, 433, 734, 797, 838, 1168
BseYI CCCAGC 1 cut(s) 1103
BsgI GTGCAG 1 cut(s) 432
Bsh1236I CGCG 2 cut(s) 443, 632
BshFI GGCC 8 cut(s) 50, 227, 328, 491, 527, 737, 903, 1039
BshNI GGYRCC 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 1054
BsiSI CCGG 5 cut(s) 329, 411, 492, 684, 954
BslFI GGGAC 3 cut(s) 617, 672, 1138
BslI CCNNNNNNNGG 1 cut(s) 899
BsmFI GGGAC 3 cut(s) 617, 672, 1138
BsnI GGCC 8 cut(s) 50, 227, 328, 491, 527, 737, 903, 1039
BsoBI CYCGRG 1 cut(s) 1054
Bsp143I GATC 3 cut(s) 390, 477, 727
Bsp19I CCATGG 1 cut(s) 1040
BspACI CCGC 8 cut(s) 345, 443, 579, 632, 635, 644, 745, 821
BspANI GGCC 8 cut(s) 50, 227, 328, 491, 527, 737, 903, 1039
BspCNI CTCAG 3 cut(s) 692, 1228, 1255
BspFNI CGCG 2 cut(s) 443, 632
BspLI GGNNCC 4 cut(s) 409, 688, 724, 985
BspMAI CTGCAG 1 cut(s) 856
BspQI GCTCTTC 1 cut(s) 717
BspT107I GGYRCC 1 cut(s) 983
BsrDI GCAATG 3 cut(s) 223, 255, 853
BsrFI RCCGGY 3 cut(s) 491, 683, 953
BsrI ACTGG 5 cut(s) 51, 228, 582, 1174, 1300
BssAI RCCGGY 3 cut(s) 491, 683, 953
BssECI CCNNGG 3 cut(s) 925, 1040, 1256
BssMI GATC 3 cut(s) 390, 477, 727
BssT1I CCWWGG 2 cut(s) 925, 1040
Bst2UI CCWGG 1 cut(s) 1257
Bst4CI ACNGT 8 cut(s) 120, 177, 376, 427, 628, 794, 829, 1063
Bst6I CTCTTC 2 cut(s) 452, 717
BstBAI YACGTR 1 cut(s) 475
BstDEI CTNAG 4 cut(s) 652, 679, 1236, 1242
BstDSI CCRYGG 1 cut(s) 1040
BstF5I GGATG 2 cut(s) 329, 520
BstFNI CGCG 2 cut(s) 443, 632
BstKTI GATC 3 cut(s) 393, 480, 730
BstMBI GATC 3 cut(s) 390, 477, 727
BstMWI GCNNNNNNNGC 7 cut(s) 334, 443, 629, 641, 1160, 1181, 1238
BstNI CCWGG 1 cut(s) 1257
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 1 cut(s) 1255
BstSFI CTRYAG 2 cut(s) 852, 1319
BstUI CGCG 2 cut(s) 443, 632
BstV1I GCAGC 6 cut(s) 410, 433, 734, 797, 838, 1168
BsuRI GGCC 8 cut(s) 50, 227, 328, 491, 527, 737, 903, 1039
BtgI CCRYGG 1 cut(s) 1040
BtgZI GCGATG 2 cut(s) 567, 765
BtsCI GGATG 2 cut(s) 329, 520
BtsIMutI CAGTG 5 cut(s) 58, 116, 221, 1167, 1233
Cfr10I RCCGGY 3 cut(s) 491, 683, 953
Cfr13I GGNCC 4 cut(s) 48, 146, 686, 901
Csp6I GTAC 5 cut(s) 763, 825, 1051, 1111, 1213
CviAII CATG 8 cut(s) 17, 229, 241, 277, 881, 913, 1041, 1203
CviQI GTAC 5 cut(s) 763, 825, 1051, 1111, 1213
DdeI CTNAG 4 cut(s) 652, 679, 1236, 1242
DpnI GATC 3 cut(s) 392, 479, 729
DpnII GATC 3 cut(s) 390, 477, 727
DraI TTTAAA 1 cut(s) 169
EaeI YGGCCR 2 cut(s) 225, 326
Eam1104I CTCTTC 2 cut(s) 452, 717
EarI CTCTTC 2 cut(s) 452, 717
Eco130I CCWWGG 2 cut(s) 925, 1040
Eco32I GATATC 2 cut(s) 877, 1090
Eco47I GGWCC 2 cut(s) 146, 686
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1054
EcoRII CCWGG 1 cut(s) 1255
EcoRV GATATC 2 cut(s) 877, 1090
EcoT14I CCWWGG 2 cut(s) 925, 1040
ErhI CCWWGG 2 cut(s) 925, 1040
FaeI CATG 8 cut(s) 20, 232, 244, 280, 884, 916, 1044, 1206
FalI AAGNNNNNCTT 2 cut(s) 1001, 1033
FaqI GGGAC 3 cut(s) 617, 672, 1138
FatI CATG 8 cut(s) 16, 228, 240, 276, 880, 912, 1040, 1202
FauI CCCGC 2 cut(s) 637, 814
FbaI TGATCA 1 cut(s) 477
FblI GTMKAC 2 cut(s) 113, 284
Fnu4HI GCNGC 9 cut(s) 345, 399, 444, 447, 633, 748, 811, 852, 1182
FokI GGATG 2 cut(s) 336, 527
Fsp4HI GCNGC 9 cut(s) 345, 399, 444, 447, 633, 748, 811, 852, 1182
FspBI CTAG 2 cut(s) 1136, 1313
GluI GCNGC 9 cut(s) 345, 399, 444, 447, 633, 748, 811, 852, 1182
GsaI CCCAGC 1 cut(s) 1107
GsuI CTGGAG 1 cut(s) 1191
HaeIII GGCC 8 cut(s) 50, 227, 328, 491, 527, 737, 903, 1039
HapII CCGG 5 cut(s) 329, 411, 492, 684, 954
Hin1II CATG 8 cut(s) 20, 232, 244, 280, 884, 916, 1044, 1206
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 3 cut(s) 483, 648, 770
HpaII CCGG 5 cut(s) 329, 411, 492, 684, 954
HphI GGTGA 3 cut(s) 658, 1210, 1231
Hpy166II GTNNAC 3 cut(s) 114, 285, 784
Hpy188I TCNGA 5 cut(s) 192, 433, 482, 727, 994
Hpy188III TCNNGA 1 cut(s) 673
Hpy8I GTNNAC 3 cut(s) 114, 285, 784
Hpy99I CGWCG 3 cut(s) 286, 289, 443
HpyAV CCTTC 8 cut(s) 102, 552, 622, 679, 748, 1003, 1039, 1201
HpyCH4III ACNGT 8 cut(s) 120, 177, 376, 427, 628, 794, 829, 1063
HpyCH4IV ACGT 2 cut(s) 474, 780
HpyCH4V TGCA 7 cut(s) 221, 260, 364, 449, 854, 1072, 1184
HpyF10VI GCNNNNNNNGC 7 cut(s) 334, 443, 629, 641, 1160, 1181, 1238
HpyF3I CTNAG 4 cut(s) 652, 679, 1236, 1242
HpySE526I ACGT 2 cut(s) 474, 780
Hsp92II CATG 8 cut(s) 20, 232, 244, 280, 884, 916, 1044, 1206
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 3 cut(s) 390, 477, 727
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 1 cut(s) 1172
Lsp1109I GCAGC 6 cut(s) 410, 433, 734, 797, 838, 1168
MaeI CTAG 2 cut(s) 1136, 1313
MaeII ACGT 2 cut(s) 474, 780
MaeIII GTNAC 4 cut(s) 376, 664, 861, 1198
MalI GATC 3 cut(s) 392, 479, 729
MboI GATC 3 cut(s) 390, 477, 727
MboII GAAGA 5 cut(s) 469, 704, 793, 832, 919
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 9 cut(s) 36, 61, 82, 105, 170, 570, 616, 895, 1261
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 764
MnlI CCTC 8 cut(s) 301, 379, 700, 785, 799, 1029, 1050, 1231
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MseI TTAA 6 cut(s) 60, 81, 104, 122, 168, 234
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 747
MspI CCGG 5 cut(s) 329, 411, 492, 684, 954
MspR9I CCNGG 1 cut(s) 1257
MvaI CCWGG 1 cut(s) 1257
MvnI CGCG 2 cut(s) 443, 632
MwoI GCNNNNNNNGC 7 cut(s) 334, 443, 629, 641, 1160, 1181, 1238
NcoI CCATGG 1 cut(s) 1040
NdeII GATC 3 cut(s) 390, 477, 727
NlaIII CATG 8 cut(s) 20, 232, 244, 280, 884, 916, 1044, 1206
NlaIV GGNNCC 4 cut(s) 409, 688, 724, 985
NmeAIII GCCGAG 2 cut(s) 713, 1030
NmuCI GTSAC 3 cut(s) 376, 664, 1198
NspI RCATGY 1 cut(s) 916
PaeR7I CTCGAG 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 483, 648
PkrI GCNGC 9 cut(s) 346, 400, 445, 448, 634, 749, 812, 853, 1183
PleI GAGTC 1 cut(s) 764
PpsI GAGTC 1 cut(s) 764
Ppu21I YACGTR 1 cut(s) 475
PshBI ATTAAT 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 1255
PspFI CCCAGC 1 cut(s) 1103
PspGI CCWGG 1 cut(s) 1255
PspN4I GGNNCC 4 cut(s) 409, 688, 724, 985
PspPI GGNCC 4 cut(s) 48, 146, 686, 901
PspXI VCTCGAGB 1 cut(s) 1054
PstI CTGCAG 1 cut(s) 856
RsaI GTAC 5 cut(s) 764, 826, 1052, 1112, 1214
RsaNI GTAC 5 cut(s) 763, 825, 1051, 1111, 1213
SalI GTCGAC 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 6 cut(s) 60, 81, 104, 122, 168, 234
SatI GCNGC 9 cut(s) 345, 399, 444, 447, 633, 748, 811, 852, 1182
Sau3AI GATC 3 cut(s) 390, 477, 727
Sau96I GGNCC 4 cut(s) 48, 146, 686, 901
ScaI AGTACT 1 cut(s) 1052
SchI GAGTC 1 cut(s) 764
ScrFI CCNGG 1 cut(s) 1257
SetI ASST 9 cut(s) 303, 477, 614, 671, 783, 1019, 1177, 1221, 1318
SfcI CTRYAG 2 cut(s) 852, 1319
Sfr274I CTCGAG 1 cut(s) 1054
SgrDI CGTCGACG 1 cut(s) 283
SinI GGWCC 2 cut(s) 146, 686
SlaI CTCGAG 1 cut(s) 1054
SmiI ATTTAAAT 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 1054
SmoI CTYRAG 1 cut(s) 1054
Sse9I AATT 9 cut(s) 36, 61, 82, 105, 170, 570, 616, 895, 1261
SsiI CCGC 8 cut(s) 345, 443, 579, 632, 635, 644, 745, 821
SspMI CTAG 2 cut(s) 1136, 1313
StyD4I CCNGG 1 cut(s) 1255
StyI CCWWGG 2 cut(s) 925, 1040
SwaI ATTTAAAT 1 cut(s) 169
TaaI ACNGT 8 cut(s) 120, 177, 376, 427, 628, 794, 829, 1063
TaiI ACGT 2 cut(s) 477, 783
TaqI TCGA 7 cut(s) 284, 455, 597, 788, 804, 831, 1055
TaqII GACCGA 1 cut(s) 163
TasI AATT 9 cut(s) 36, 61, 82, 105, 170, 570, 616, 895, 1261
TatI WGTACW 1 cut(s) 1050
TauI GCSGC 3 cut(s) 347, 446, 635
TfiI GAWTC 2 cut(s) 483, 648
Tru1I TTAA 6 cut(s) 60, 81, 104, 122, 168, 234
Tru9I TTAA 6 cut(s) 60, 81, 104, 122, 168, 234
TscAI CASTG 5 cut(s) 58, 123, 228, 1174, 1240
TseFI GTSAC 3 cut(s) 376, 664, 1198
TseI GCWGC 6 cut(s) 398, 446, 747, 810, 851, 1181
Tsp45I GTSAC 3 cut(s) 376, 664, 1198
TspDTI ATGAA 2 cut(s) 284, 826
TspGWI ACGGA 2 cut(s) 336, 420
TspRI CASTG 5 cut(s) 58, 123, 228, 1174, 1240
VpaK11BI GGWCC 2 cut(s) 146, 686
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 1 cut(s) 1261
XceI RCATGY 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 2 cut(s) 589, 994
XhoI CTCGAG 1 cut(s) 1054
XmiI GTMKAC 2 cut(s) 113, 284
XspI CTAG 2 cut(s) 1136, 1313
ZrmI AGTACT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.