Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0012707

Physical Location & Seq

Genomic Coordinates Reverse (-)

63250226 .. 63255556

Gene Structure 5331 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020287

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGGCCACAATCAAAGAGTTTCTGGCCAAAGTGTCCCAATCCAAAAAGCACAAGAAGCTCTTCTTGGCTCTCATAGCCACGGTTCTACTAGTCGCCGCAGTTATCGGCATAGTCACCGGAGTTAAAAACAAAAACTCCAACTCCGACAACGAGTCCCTCTCCGCCGCTCACGCCATTGTCAAATTCTCGTGCAGCTCCACCCTCTACCCCGAGCTCTGCTTCTCCACCATCGCCACGTCAGCCGCCACCACAAACGTCACCAGCCTCAAAGACGTGATCGAGCTGTCGCTGAACATCACCATCACGGCGGTGGAGCACAACTTCTTCACCATCGAGAAGCTGATCAAGAAGCTCGTGAAGAGCCTCACGAAGCGCGAGAGGGCTGCCTTGCACGACTGCCTGGAGACCATTGACGAGACGCTCGATGAGCTCCACGAGGCGGTAAAGGATCTGTTTCAGTACCCGAGCAACAACAGGACTCTGACTGCGCACGCCGACGATCTCAAGACGTTGATAAGCTCCGCCATTACCAACCAGGAGACTTGTCTCGACGGGTTTTCTCACGCCGAGGCTGATAAAATAGTTCGGAAATCGTTGGAGAAGGGTCAGAAATATGTAGAAAAGTTGTGCAGCAATGCACTGGCCATGATTAAAAACATGACTGATAACGACATTGCAAACGAGATGAAAGCCATGTCCTTGACGACTAACCGAAAGCTAAAGGAGGAACATTCAAACGGATGGCCGGAGTGGCTGTCTGCCGCAGATAGACATCTCTTGCAATCCTCTACTATCCCACCGAATGTGATCGTGGCAGCAGACGGAACCGGAGATTACAAGACGGTTTCGGAAGCCGTCGCGGCTGCACCATCGAAGAGTAGCACCAGATACGTGATCCGAATCAAGGCCGGTGTGTATAGAGAGAATGTGGATGTGCCAAAGGCCAAGACGAACATAATGTTCTTGGGCGATGACAGGAGAACCACAATTATTACCGCAAGTAGAAATGTGGTCGATGGGGACACGACCTTCAATTCTGCCACAGTTGCGGCGGTTGGTGCGGGATTCTTAGCAAAAGAAGTCACCTTCCAGAACTCAGCCGGTCCCTCAAAACACCAAGCAGTTGCTCTTCGTGTTGGTTCCGATCTCTCGGCCTTCTACCGCTGCGATATTATTGCGTACCAAGACTCCCTCTACGTCCACTCGAACCGTCAGTTCTTCGAAGGCTGCTTTGTGGCGGGTACAGTCGATTTCATCTTCGGCAATGCTGCAGTCGTGTTACAAAACTGCGATATCCATGCCCGAAAGCCCAATTCGGGCCAGAAGAACATGCTCACAGCCCAAGGGAGAACCGACCCCAACCAGAACACTGGCATTGTGATCCAAGAATCAAGGATTGGTGCCACTTCTGACTTGGAAGCCGAGAAGGGTAGCTTCAAAACATATCTTGGGAGGCCATGGAAGGAGTACTCGAGGACTGTGATTATGCAATCATCCATTAGTGATATCATAGACCCTGCTGGGTGGTACGAGTGGAGTGGGACTTTTGCCCTAGACACATTATTCTATGCCGAGTACGCTAACACTGGAGCTGGTGCTGCAACCACGAACAGGGTGACATGGAAGGGGTACAAGGTGATTACTAGTCCCACTGAGGGTCTGGCTTACACACCAGGGAATTTTATTGCTGGTGGAAGTTGGTTGAATGCTACTGGTTTTCCATTTACTCTTGGTCTGTCGAATGTAGACGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

585

Amino Acids

63.63

Weight (kDa)

6.87

Isoelectric Point (pI)

22.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	59 - 216	6.8e-34	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	268 - 564	1.3e-141	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012707)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53830 AT3G14310
fragaria_vesca	FvH4_6g35830
malus_domestica	MD09G1172600.v1.1 MD17G1154500.v1.1
prunus_persica	Prupe.3G031000_v2.0.a1
pyrus_communis	pycom09g09010 pycom09g09020
rosa_chinensis	RchiOBHm_Chr2g0147881
rosa_laevigata	RLG00000020287
rosa_multiflora	Rmu_sc0000308.1_g000057
rosa_roxburghii	Rroxscaffold_2G00099240
rosa_rugosa	Rorug02G0402600
rosa_samantha	Rh2AG459500 Rh2BG472900 Rh2CG446900 Rh2DG481800
rosa_wichuraiana	Rw2G037590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	489
AccB1I	GGYRCC	1 cut(s)	1400
AccBSI	CCGCTC	1 cut(s)	167
AccI	GTMKAC	1 cut(s)	1746
AccII	CGCG	2 cut(s)	375, 860
AclWI	GGATC	3 cut(s)	456, 889, 1375
AcoI	YGGCCR	4 cut(s)	3, 24, 642, 743
AcsI	RAATTY	2 cut(s)	182, 1678
AfaI	GTAC	7 cut(s)	461, 1181, 1243, 1469, 1529, 1577, 1631
AfiI	CCNNNNNNNGG	5 cut(s)	439, 904, 1316, 1611, 1655
AgsI	TTSAA	4 cut(s)	735, 1033, 1438, 1705
AhdI	GACNNNNNGTC	1 cut(s)	151
AhlI	ACTAGT	2 cut(s)	88, 1643
AjiI	CACGTC	2 cut(s)	237, 274
AjnI	CCWGG	3 cut(s)	399, 534, 1672
AjuI	GAANNNNNNNTTGG	4 cut(s)	47, 79, 1380, 1412
AleI	CACNNNNGTG	1 cut(s)	308
Alw21I	GWGCWC	3 cut(s)	216, 318, 432
Alw26I	GTCTC	4 cut(s)	398, 410, 533, 551
AlwI	GGATC	3 cut(s)	456, 889, 1375
Ama87I	CYCGRG	3 cut(s)	209, 463, 1471
AoxI	GGCC	9 cut(s)	3, 24, 642, 743, 906, 942, 1152, 1318, 1454
ApeKI	GCWGC	9 cut(s)	192, 383, 630, 815, 863, 1164, 1227, 1268, 1598
ApoI	RAATTY	2 cut(s)	182, 1678
Asp700I	GAANNNNTTC	1 cut(s)	59
AspLEI	GCGC	2 cut(s)	375, 490
AspS9I	GGNCC	2 cut(s)	1103, 1318
AsuHPI	GGTGA	7 cut(s)	106, 250, 289, 319, 1075, 1627, 1648
AsuII	TTCGAA	1 cut(s)	1221
AvaI	CYCGRG	3 cut(s)	209, 463, 1471
AvaII	GGWCC	1 cut(s)	1103
BalI	TGGCCA	3 cut(s)	5, 26, 644
BanI	GGYRCC	1 cut(s)	1400
BanII	GRGCYC	2 cut(s)	216, 432
BauI	CACGAG	3 cut(s)	187, 353, 434
Bbv12I	GWGCWC	3 cut(s)	216, 318, 432
BbvI	GCAGC	9 cut(s)	204, 370, 642, 827, 850, 1151, 1214, 1255, 1585
BccI	CCATC	6 cut(s)	236, 308, 338, 735, 877, 1010
BceAI	ACGGC	2 cut(s)	321, 839
BcgI	CGANNNNNNTGC	6 cut(s)	365, 399, 1157, 1191, 1280, 1314
BciT130I	CCWGG	3 cut(s)	401, 536, 1674
BclI	TGATCA	1 cut(s)	342
BcoDI	GTCTC	4 cut(s)	398, 410, 533, 551
BcuI	ACTAGT	2 cut(s)	88, 1643
BfaI	CTAG	3 cut(s)	89, 1553, 1644
BfmI	CTRYAG	1 cut(s)	1269
BglI	GCCNNNNNGGC	2 cut(s)	751, 860
BmcAI	AGTACT	1 cut(s)	1469
Bme1390I	CCNGG	3 cut(s)	401, 536, 1674
Bme18I	GGWCC	1 cut(s)	1103
BmeRI	GACNNNNNGTC	1 cut(s)	151
BmeT110I	CYCGRG	3 cut(s)	209, 463, 1471
BmgBI	CACGTC	2 cut(s)	237, 274
BmgT120I	GGNCC	2 cut(s)	1103, 1318
BmiI	GGNNCC	4 cut(s)	826, 1105, 1141, 1402
BmrFI	CCNGG	3 cut(s)	401, 536, 1674
BplI	GAGNNNNNCTC	2 cut(s)	143, 175
BpmI	CTGGAG	2 cut(s)	422, 1608
Bpu14I	TTCGAA	1 cut(s)	1221
BpuEI	CTTGAG	1 cut(s)	488
BsaAI	YACGTR	1 cut(s)	892
BsaBI	GATNNNNATC	2 cut(s)	771, 899
BsaI	GGTCTC	1 cut(s)	398
BsaJI	CCNNGG	5 cut(s)	78, 567, 1342, 1457, 1673
BsaWI	WCCGGW	2 cut(s)	116, 827
BsaXI	ACNNNNNCTCC	8 cut(s)	119, 125, 149, 155, 740, 770, 1172, 1202
Bsc4I	CCNNNNNNNGG	5 cut(s)	439, 904, 1316, 1611, 1655
Bse118I	RCCGGY	2 cut(s)	908, 1100
Bse1I	ACTGG	4 cut(s)	645, 1375, 1591, 1717
Bse3DI	GCAATG	3 cut(s)	640, 672, 1270
Bse8I	GATNNNNATC	2 cut(s)	771, 899
BseBI	CCWGG	3 cut(s)	401, 536, 1674
BseDI	CCNNGG	5 cut(s)	78, 567, 1342, 1457, 1673
BseGI	GGATG	3 cut(s)	746, 937, 1493
BseJI	GATNNNNATC	2 cut(s)	771, 899
BseLI	CCNNNNNNNGG	5 cut(s)	439, 904, 1316, 1611, 1655
BseMI	GCAATG	3 cut(s)	640, 672, 1270
BseMII	CTCAG	2 cut(s)	1110, 1644
BseNI	ACTGG	4 cut(s)	645, 1375, 1591, 1717
BseXI	GCAGC	9 cut(s)	204, 370, 642, 827, 850, 1151, 1214, 1255, 1585
BseYI	CCCAGC	1 cut(s)	1520
BsgI	GTGCAG	3 cut(s)	211, 649, 849
Bsh1236I	CGCG	2 cut(s)	375, 860
BshFI	GGCC	9 cut(s)	5, 26, 644, 745, 908, 944, 1154, 1320, 1456
BshNI	GGYRCC	1 cut(s)	1400
BsiHKAI	GWGCWC	3 cut(s)	216, 318, 432
BsiHKCI	CYCGRG	3 cut(s)	209, 463, 1471
BsiSI	CCGG	5 cut(s)	117, 746, 828, 909, 1101
BslFI	GGGAC	6 cut(s)	19, 139, 1034, 1089, 1555, 1632
BslI	CCNNNNNNNGG	5 cut(s)	439, 904, 1316, 1611, 1655
BsmAI	GTCTC	4 cut(s)	398, 410, 533, 551
BsmBI	CGTCTC	1 cut(s)	410
BsmFI	GGGAC	6 cut(s)	19, 139, 1034, 1089, 1555, 1632
BsmI	GAATGC	1 cut(s)	1711
BsnI	GGCC	9 cut(s)	5, 26, 644, 745, 908, 944, 1154, 1320, 1456
Bso31I	GGTCTC	1 cut(s)	398
BsoBI	CYCGRG	3 cut(s)	209, 463, 1471
Bsp119I	TTCGAA	1 cut(s)	1221
Bsp1286I	GDGCHC	3 cut(s)	216, 318, 432
Bsp143I	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
Bsp19I	CCATGG	1 cut(s)	1457
BspANI	GGCC	9 cut(s)	5, 26, 644, 745, 908, 944, 1154, 1320, 1456
BspCNI	CTCAG	2 cut(s)	1109, 1645
BspFNI	CGCG	2 cut(s)	375, 860
BspLI	GGNNCC	4 cut(s)	826, 1105, 1141, 1402
BspMAI	CTGCAG	1 cut(s)	1273
BspPI	GGATC	3 cut(s)	456, 889, 1375
BspQI	GCTCTTC	3 cut(s)	65, 353, 1134
BspT104I	TTCGAA	1 cut(s)	1221
BspT107I	GGYRCC	1 cut(s)	1400
BspTNI	GGTCTC	1 cut(s)	398
BsrBI	CCGCTC	1 cut(s)	167
BsrDI	GCAATG	3 cut(s)	640, 672, 1270
BsrFI	RCCGGY	2 cut(s)	908, 1100
BsrI	ACTGG	4 cut(s)	645, 1375, 1591, 1717
BssAI	RCCGGY	2 cut(s)	908, 1100
BssECI	CCNNGG	5 cut(s)	78, 567, 1342, 1457, 1673
BssMI	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
BssSI	CACGAG	3 cut(s)	187, 353, 434
BssT1I	CCWWGG	2 cut(s)	1342, 1457
Bst2BI	CACGAG	3 cut(s)	187, 353, 434
Bst2UI	CCWGG	3 cut(s)	401, 536, 1674
Bst4CI	ACNGT	6 cut(s)	82, 844, 1045, 1211, 1246, 1480
Bst6I	CTCTTC	4 cut(s)	65, 353, 869, 1134
BstBAI	YACGTR	1 cut(s)	892
BstBI	TTCGAA	1 cut(s)	1221
BstC8I	GCNNGC	1 cut(s)	492
BstDEI	CTNAG	3 cut(s)	1069, 1096, 1653
BstDSI	CCRYGG	2 cut(s)	78, 1457
BstF5I	GGATG	3 cut(s)	746, 937, 1493
BstFNI	CGCG	2 cut(s)	375, 860
BstHHI	GCGC	2 cut(s)	375, 490
BstKTI	GATC	8 cut(s)	279, 345, 451, 502, 810, 897, 1147, 1383
BstMAI	GTCTC	4 cut(s)	398, 410, 533, 551
BstMBI	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
BstNI	CCWGG	3 cut(s)	401, 536, 1674
BstNSI	RCATGY	1 cut(s)	1333
BstSCI	CCNGG	3 cut(s)	399, 534, 1672
BstSFI	CTRYAG	1 cut(s)	1269
BstUI	CGCG	2 cut(s)	375, 860
BstV1I	GCAGC	9 cut(s)	204, 370, 642, 827, 850, 1151, 1214, 1255, 1585
BstX2I	RGATCY	1 cut(s)	448
BstXI	CCANNNNNNTGG	1 cut(s)	1370
BstYI	RGATCY	1 cut(s)	448
BsuRI	GGCC	9 cut(s)	5, 26, 644, 745, 908, 944, 1154, 1320, 1456
BtgI	CCRYGG	2 cut(s)	78, 1457
BtgZI	GCGATG	2 cut(s)	214, 984
BtrI	CACGTC	2 cut(s)	237, 274
BtsCI	GGATG	3 cut(s)	746, 937, 1493
BtsIMutI	CAGTG	4 cut(s)	638, 1368, 1584, 1650
Cac8I	GCNNGC	1 cut(s)	492
CfoI	GCGC	2 cut(s)	375, 490
Cfr10I	RCCGGY	2 cut(s)	908, 1100
Cfr13I	GGNCC	2 cut(s)	1103, 1318
CseI	GACGC	1 cut(s)	427
Csp6I	GTAC	7 cut(s)	460, 1180, 1242, 1468, 1528, 1576, 1630
CviAII	CATG	7 cut(s)	646, 658, 694, 1298, 1330, 1458, 1620
CviQI	GTAC	7 cut(s)	460, 1180, 1242, 1468, 1528, 1576, 1630
DdeI	CTNAG	3 cut(s)	1069, 1096, 1653
DpnI	GATC	8 cut(s)	278, 344, 450, 501, 809, 896, 1146, 1382
DpnII	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
DriI	GACNNNNNGTC	1 cut(s)	151
EaeI	YGGCCR	4 cut(s)	3, 24, 642, 743
Eam1104I	CTCTTC	4 cut(s)	65, 353, 869, 1134
Eam1105I	GACNNNNNGTC	1 cut(s)	151
EarI	CTCTTC	4 cut(s)	65, 353, 869, 1134
EciI	GGCGGA	2 cut(s)	151, 511
Ecl136II	GAGCTC	2 cut(s)	214, 430
Eco130I	CCWWGG	2 cut(s)	1342, 1457
Eco24I	GRGCYC	2 cut(s)	216, 432
Eco31I	GGTCTC	1 cut(s)	398
Eco32I	GATATC	2 cut(s)	1294, 1507
Eco47I	GGWCC	1 cut(s)	1103
Eco53kI	GAGCTC	2 cut(s)	214, 430
Eco88I	CYCGRG	3 cut(s)	209, 463, 1471
EcoICRI	GAGCTC	2 cut(s)	214, 430
EcoRII	CCWGG	3 cut(s)	399, 534, 1672
EcoRV	GATATC	2 cut(s)	1294, 1507
EcoT14I	CCWWGG	2 cut(s)	1342, 1457
EcoT38I	GRGCYC	2 cut(s)	216, 432
ErhI	CCWWGG	2 cut(s)	1342, 1457
Esp3I	CGTCTC	1 cut(s)	410
FaeI	CATG	7 cut(s)	649, 661, 697, 1301, 1333, 1461, 1623
FalI	AAGNNNNNCTT	6 cut(s)	44, 76, 47, 79, 1418, 1450
FaqI	GGGAC	6 cut(s)	19, 139, 1034, 1089, 1555, 1632
FatI	CATG	7 cut(s)	645, 657, 693, 1297, 1329, 1457, 1619
FauI	CCCGC	2 cut(s)	1054, 1231
FbaI	TGATCA	1 cut(s)	342
FblI	GTMKAC	1 cut(s)	1746
FokI	GGATG	3 cut(s)	753, 944, 1480
FriOI	GRGCYC	2 cut(s)	216, 432
FspBI	CTAG	3 cut(s)	89, 1553, 1644
FspI	TGCGCA	1 cut(s)	489
GlaI	GCGC	2 cut(s)	374, 489
GsaI	CCCAGC	1 cut(s)	1524
GsuI	CTGGAG	2 cut(s)	422, 1608
HaeIII	GGCC	9 cut(s)	5, 26, 644, 745, 908, 944, 1154, 1320, 1456
HapII	CCGG	5 cut(s)	117, 746, 828, 909, 1101
HgaI	GACGC	1 cut(s)	427
HhaI	GCGC	2 cut(s)	375, 490
Hin1II	CATG	7 cut(s)	649, 661, 697, 1301, 1333, 1461, 1623
Hin6I	GCGC	2 cut(s)	373, 488
HinP1I	GCGC	2 cut(s)	373, 488
HinfI	GANTC	6 cut(s)	152, 478, 900, 1065, 1187, 1388
HpaII	CCGG	5 cut(s)	117, 746, 828, 909, 1101
HphI	GGTGA	7 cut(s)	106, 250, 289, 319, 1075, 1627, 1648
Hpy166II	GTNNAC	2 cut(s)	1201, 1747
Hpy188I	TCNGA	8 cut(s)	145, 483, 588, 609, 850, 899, 1144, 1411
Hpy188III	TCNNGA	7 cut(s)	334, 346, 355, 367, 505, 548, 1090
Hpy8I	GTNNAC	2 cut(s)	1201, 1747
Hpy99I	CGWCG	3 cut(s)	500, 554, 860
HpyAV	CCTTC	8 cut(s)	595, 1039, 1096, 1165, 1217, 1420, 1456, 1618
HpyCH4III	ACNGT	6 cut(s)	82, 844, 1045, 1211, 1246, 1480
HpyCH4IV	ACGT	6 cut(s)	236, 255, 273, 509, 891, 1197
HpyF3I	CTNAG	3 cut(s)	1069, 1096, 1653
HpySE526I	ACGT	6 cut(s)	236, 255, 273, 509, 891, 1197
Hsp92II	CATG	7 cut(s)	649, 661, 697, 1301, 1333, 1461, 1623
HspAI	GCGC	2 cut(s)	373, 488
Ksp22I	TGATCA	1 cut(s)	342
Kzo9I	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
LguI	GCTCTTC	3 cut(s)	65, 353, 1134
LmnI	GCTCC	5 cut(s)	200, 313, 435, 524, 1589
Lsp1109I	GCAGC	9 cut(s)	204, 370, 642, 827, 850, 1151, 1214, 1255, 1585
MaeI	CTAG	3 cut(s)	89, 1553, 1644
MaeII	ACGT	6 cut(s)	236, 255, 273, 509, 891, 1197
MaeIII	GTNAC	5 cut(s)	112, 256, 1081, 1278, 1615
MalI	GATC	8 cut(s)	278, 344, 450, 501, 809, 896, 1146, 1382
MbiI	CCGCTC	1 cut(s)	167
MboI	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
MboII	GAAGA	8 cut(s)	52, 316, 370, 886, 1121, 1210, 1249, 1336
MflI	RGATCY	1 cut(s)	448
MhlI	GDGCHC	3 cut(s)	216, 318, 432
MlsI	TGGCCA	3 cut(s)	5, 26, 644
MluCI	AATT	5 cut(s)	182, 987, 1033, 1312, 1678
MluNI	TGGCCA	3 cut(s)	5, 26, 644
MlyI	GAGTC	3 cut(s)	161, 472, 1181
MmeI	TCCRAC	3 cut(s)	162, 168, 576
Mox20I	TGGCCA	3 cut(s)	5, 26, 644
MroXI	GAANNNNTTC	1 cut(s)	59
MscI	TGGCCA	3 cut(s)	5, 26, 644
MseI	TTAA	2 cut(s)	123, 651
MslI	CAYNNNNRTG	1 cut(s)	308
Msp20I	TGGCCA	3 cut(s)	5, 26, 644
MspA1I	CMGCKG	1 cut(s)	1164
MspI	CCGG	5 cut(s)	117, 746, 828, 909, 1101
MspR9I	CCNGG	3 cut(s)	401, 536, 1674
Mva1269I	GAATGC	1 cut(s)	1711
MvaI	CCWGG	3 cut(s)	401, 536, 1674
MvnI	CGCG	2 cut(s)	375, 860
NcoI	CCATGG	1 cut(s)	1457
NdeII	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
NlaIII	CATG	7 cut(s)	649, 661, 697, 1301, 1333, 1461, 1623
NlaIV	GGNNCC	4 cut(s)	826, 1105, 1141, 1402
NmeAIII	GCCGAG	4 cut(s)	592, 1130, 1447, 1597
NmuCI	GTSAC	4 cut(s)	112, 256, 1081, 1615
NsbI	TGCGCA	1 cut(s)	489
NspI	RCATGY	1 cut(s)	1333
NspV	TTCGAA	1 cut(s)	1221
OliI	CACNNNNGTG	1 cut(s)	308
PaeR7I	CTCGAG	1 cut(s)	1471
PciSI	GCTCTTC	3 cut(s)	65, 353, 1134
PcsI	WCGNNNNNNNCGW	1 cut(s)	420
PctI	GAATGC	1 cut(s)	1711
PdmI	GAANNNNTTC	1 cut(s)	59
PfeI	GAWTC	3 cut(s)	900, 1065, 1388
PleI	GAGTC	3 cut(s)	160, 472, 1181
PpsI	GAGTC	3 cut(s)	160, 472, 1181
Ppu21I	YACGTR	1 cut(s)	892
Psp124BI	GAGCTC	2 cut(s)	216, 432
Psp6I	CCWGG	3 cut(s)	399, 534, 1672
PspFI	CCCAGC	1 cut(s)	1520
PspGI	CCWGG	3 cut(s)	399, 534, 1672
PspN4I	GGNNCC	4 cut(s)	826, 1105, 1141, 1402
PspPI	GGNCC	2 cut(s)	1103, 1318
PspXI	VCTCGAGB	1 cut(s)	1471
PstI	CTGCAG	1 cut(s)	1273
PsuI	RGATCY	1 cut(s)	448
RsaI	GTAC	7 cut(s)	461, 1181, 1243, 1469, 1529, 1577, 1631
RsaNI	GTAC	7 cut(s)	460, 1180, 1242, 1468, 1528, 1576, 1630
RseI	CAYNNNNRTG	1 cut(s)	308
SacI	GAGCTC	2 cut(s)	216, 432
SapI	GCTCTTC	3 cut(s)	65, 353, 1134
SaqAI	TTAA	2 cut(s)	123, 651
Sau3AI	GATC	8 cut(s)	276, 342, 448, 499, 807, 894, 1144, 1380
Sau96I	GGNCC	2 cut(s)	1103, 1318
ScaI	AGTACT	1 cut(s)	1469
SchI	GAGTC	3 cut(s)	161, 472, 1181
ScrFI	CCNGG	3 cut(s)	401, 536, 1674
SduI	GDGCHC	3 cut(s)	216, 318, 432
SfcI	CTRYAG	1 cut(s)	1269
Sfr274I	CTCGAG	1 cut(s)	1471
SfuI	TTCGAA	1 cut(s)	1221
SinI	GGWCC	1 cut(s)	1103
SlaI	CTCGAG	1 cut(s)	1471
SmiMI	CAYNNNNRTG	1 cut(s)	308
SmlI	CTYRAG	2 cut(s)	503, 1471
SmoI	CTYRAG	2 cut(s)	503, 1471
SpeI	ACTAGT	2 cut(s)	88, 1643
Sse9I	AATT	5 cut(s)	182, 987, 1033, 1312, 1678
SspMI	CTAG	3 cut(s)	89, 1553, 1644
SstI	GAGCTC	2 cut(s)	216, 432
StyD4I	CCNGG	3 cut(s)	399, 534, 1672
StyI	CCWWGG	2 cut(s)	1342, 1457
TaaI	ACNGT	6 cut(s)	82, 844, 1045, 1211, 1246, 1480
TaiI	ACGT	6 cut(s)	239, 258, 276, 512, 894, 1200
TasI	AATT	5 cut(s)	182, 987, 1033, 1312, 1678
TatI	WGTACW	1 cut(s)	1467
TauI	GCSGC	6 cut(s)	98, 167, 245, 764, 863, 1052
TfiI	GAWTC	3 cut(s)	900, 1065, 1388
Tru1I	TTAA	2 cut(s)	123, 651
Tru9I	TTAA	2 cut(s)	123, 651
TscAI	CASTG	4 cut(s)	645, 1375, 1591, 1657
TseFI	GTSAC	4 cut(s)	112, 256, 1081, 1615
TseI	GCWGC	9 cut(s)	192, 383, 630, 815, 863, 1164, 1227, 1268, 1598
Tsp45I	GTSAC	4 cut(s)	112, 256, 1081, 1615
TspDTI	ATGAA	2 cut(s)	701, 1243
TspGWI	ACGGA	2 cut(s)	753, 837
TspRI	CASTG	4 cut(s)	645, 1375, 1591, 1657
VpaK11BI	GGWCC	1 cut(s)	1103
XapI	RAATTY	2 cut(s)	182, 1678
XceI	RCATGY	1 cut(s)	1333
XcmI	CCANNNNNNNNNTGG	3 cut(s)	307, 1411, 1657
XhoI	CTCGAG	1 cut(s)	1471
XmiI	GTMKAC	1 cut(s)	1746
XmnI	GAANNNNTTC	1 cut(s)	59
XspI	CTAG	3 cut(s)	89, 1553, 1644
ZrmI	AGTACT	1 cut(s)	1469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.