Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012707

Physical Location & Seq

Genomic Coordinates Forward (+)

14589583 .. 14594272

Gene Structure 4690 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1172600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCCAACATCAAAGAGTTTTTAGCCAGAATGTCCGAATCAGGCAAAAAATTATCCACAAACAAGAAGAACAAGAAGCTCTTCATATCCCTTTTCGCAACCATCCTACTCGTGGGCGCCGTCATCGGCATTGTCACCGGAGTAAAGTCCTCCAAAAACAACTCCGACGACGAAACCATCGAGGCCTCCCACGCCATAGTCAAATCCTCATGCAGCTCCACCCTCTACCCTGATCTGTGCTTTTCAACTCTTGCCCTTCACCCGGAAGCCTCCAAAAAGGTTTCGAGCCAGAAGGACGTGATAGAATTGTCACTGAACATCACGACCACGGCGGTGCAGCACATCTTCTTCACCGTCGAGAAGCTGCTGAAGTCCAGGAAGAACAAGCTCACTAAGCGTGAGAAGGGCGCTCTCCACGACTGTTTGGAGACCATTGACGAAACCCTCGACGAGCTCCACGAGGCTGTCGAGGATCTCCATGAGTACCCCAATAACAAGACTTTGGTTCAGCATGCTGATGACCTTAAAACTTTGCTAAGCTCCGCCATTACCAACCAAGAAACTTGCCTTAATGGGTTCTCTCATGATGGAGGTGATAAGAAAGTTAGGAAAGTTTTGTTGGCTGGTGAGGAACACATAGAAAAATTGTGCAGTAATGTACTGGCCATGATCAAGAACATGACCGATACCGACATTGCAAACGAGATGAAAATGAATGTGAACCGAAAACTCAAGGAGAATATGGTGGACGAGCACGGGTGGCCGGAGTGGCTGTCGGTGGCGGACAGGCGACTGCTGCAGTCGTCTTCTGTCACGCCGAATGTGGTGGTGGCAGCAGACGGCACTGGGAACTACAAGACGGTGTCGGAAGCGGTGGCTGCTGCCCCAGAGAAGAGTAGTAGCAGATACGTGATTAAAATAAAGGCGGGGATTTATAGAGAAAATGTGGACGTGCCAAAGAAAAAGACCAACATTATGTTTTTGGGAGATGGGAGGGTAAACACCATCATCACTGCTAGTAGAAATGTCGTCGATGGCAGCACAACTTTCAACTCAGCCACAGTTGCTGCGGTTGGTGAGAAGTTTCTAGCCCGAGACATAACATTCCAAAACACAGCAGGCCCATCAAAGCACCAAGCTGTTGCCCTAAGAGTTGGGTCCGATCTCTCAGCATTCTACCGCTGCGACATTCTTGCGTACCAAGACTCGCTCTACGTGCACTCGAATCGCCAGTTTTTTGAAGGCTGCTTGGTAGCCGGAACAGTTGACTTCATCTTCGGCAATGCTGCTGTCGTGTTACAAAATTGTGACATCCATGCAAGAAAACCTGATTCCGGCCAGAAGAACATGGTCACTGCACAAGGTAGAACTGACCCGAATCAAAACACCGGCATCGTGATCCAAAAATCGAGAATCGGCGCCACTTCCGACTTGCAAGCCTCAAAGAGCAGCTTCAAAACGTTTTTGGGGAGGCCATGGAAGGAGTATTCAAGGACTGTGATCATGCAGTCGAGTATAACTGATATCATTGACCCTGCTGGATGGCATGAGTGGTCTGGCACTTTTGCCCTCGACACTTTGTTTTATGGAGAGTATGCTAACACTGGGGCAGGCGCTGGGACTGCAAACAGGGTGACGTGGAAGGGGTTTAAGGTAATTACAAGTCCCACTGAGGCTGAGGCGTATACGCCGGGCAACTTTATTGCTGGTGGTAGTTGGTTGAGTTCTACCGGTTTTCCATTTTCTCTTGCGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

586

Amino Acids

63.95

Weight (kDa)

8.23

Isoelectric Point (pI)

29.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	66 - 223	6.1e-37	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	274 - 570	1.3e-146	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012707)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53830 AT3G14310
fragaria_vesca	FvH4_6g35830
malus_domestica	MD09G1172600.v1.1 MD17G1154500.v1.1
prunus_persica	Prupe.3G031000_v2.0.a1
pyrus_communis	pycom09g09010 pycom09g09020
rosa_chinensis	RchiOBHm_Chr2g0147881
rosa_laevigata	RLG00000020287
rosa_multiflora	Rmu_sc0000308.1_g000057
rosa_roxburghii	Rroxscaffold_2G00099240
rosa_rugosa	Rorug02G0402600
rosa_samantha	Rh2AG459500 Rh2BG472900 Rh2CG446900 Rh2DG481800
rosa_wichuraiana	Rw2G037590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	116, 1418
AccI	GTMKAC	1 cut(s)	1685
AciI	CCGC	7 cut(s)	332, 543, 782, 872, 926, 1070, 1180
AclI	AACGTT	1 cut(s)	1460
AclWI	GGATC	2 cut(s)	480, 1393
AcoI	YGGCCR	4 cut(s)	3, 663, 761, 1336
AcuI	CTGAAG	1 cut(s)	389
AcyI	GRCGYC	2 cut(s)	117, 1419
AfaI	GTAC	3 cut(s)	485, 660, 1199
AfiI	CCNNNNNNNGG	3 cut(s)	112, 262, 1334
AgeI	ACCGGT	1 cut(s)	1730
AgsI	TTSAA	5 cut(s)	246, 1051, 1241, 1456, 1491
AjiI	CACGTC	3 cut(s)	298, 952, 1638
AjnI	CCWGG	1 cut(s)	374
AjuI	GAANNNNNNNTTGG	2 cut(s)	602, 634
AleI	CACNNNNGTG	1 cut(s)	332
AluBI	AGCT	8 cut(s)	79, 216, 364, 388, 454, 540, 1139, 1452
AluI	AGCT	8 cut(s)	79, 216, 364, 388, 454, 540, 1139, 1452
Alw21I	GWGCWC	3 cut(s)	456, 756, 1221
Alw26I	GTCTC	2 cut(s)	422, 1089
Alw44I	GTGCAC	1 cut(s)	1217
AlwI	GGATC	2 cut(s)	480, 1393
AlwNI	CAGNNNCTG	2 cut(s)	1067, 1616
Ama87I	CYCGRG	1 cut(s)	1092
AoxI	GGCC	7 cut(s)	3, 183, 663, 761, 1120, 1336, 1472
ApaLI	GTGCAC	1 cut(s)	1217
ArsI	GACNNNNNNTTYG	2 cut(s)	1259, 1291
AsiGI	ACCGGT	1 cut(s)	1730
Asp700I	GAANNNNTTC	1 cut(s)	80
AspLEI	GCGC	5 cut(s)	119, 410, 1421, 1616, 1753
AspS9I	GGNCC	2 cut(s)	1121, 1158
AsuC2I	CCSGG	2 cut(s)	263, 1692
AsuHPI	GGTGA	7 cut(s)	127, 251, 343, 605, 638, 1088, 1645
AvaI	CYCGRG	1 cut(s)	1092
AvaII	GGWCC	1 cut(s)	1158
BaeGI	GKGCMC	1 cut(s)	1221
BalI	TGGCCA	2 cut(s)	5, 665
BanI	GGYRCC	2 cut(s)	116, 1418
BanII	GRGCYC	1 cut(s)	456
BauI	CACGAG	2 cut(s)	110, 458
BbsI	GAAGAC	1 cut(s)	798
Bbv12I	GWGCWC	3 cut(s)	456, 756, 1221
BbvCI	CCTCAGC	1 cut(s)	1677
BccI	CCATC	8 cut(s)	110, 185, 581, 983, 1013, 1028, 1132, 1536
BceAI	ACGGC	3 cut(s)	104, 345, 856
BcgI	CGANNNNNNTGC	4 cut(s)	1175, 1208, 1209, 1242
BciT130I	CCWGG	1 cut(s)	376
BclI	TGATCA	2 cut(s)	669, 1500
BcnI	CCSGG	2 cut(s)	263, 1692
BcoDI	GTCTC	2 cut(s)	422, 1089
BfaI	CTAG	2 cut(s)	1017, 1088
BfmI	CTRYAG	1 cut(s)	797
BfoI	RGCGCY	4 cut(s)	120, 411, 1422, 1617
BglI	GCCNNNNNGGC	1 cut(s)	769
BlpI	GCTNAGC	1 cut(s)	536
Bme1390I	CCNGG	3 cut(s)	263, 376, 1692
Bme18I	GGWCC	1 cut(s)	1158
BmeT110I	CYCGRG	1 cut(s)	1092
BmgBI	CACGTC	3 cut(s)	298, 952, 1638
BmgT120I	GGNCC	2 cut(s)	1121, 1158
BmiI	GGNNCC	3 cut(s)	118, 1159, 1420
BmrFI	CCNGG	3 cut(s)	263, 376, 1692
BmrI	ACTGGG	2 cut(s)	855, 1614
BmsI	GCATC	1 cut(s)	1401
BmuI	ACTGGG	2 cut(s)	855, 1614
BpiI	GAAGAC	1 cut(s)	798
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu1102I	GCTNAGC	1 cut(s)	536
BpuEI	CTTGAG	1 cut(s)	716
BpuMI	CCSGG	2 cut(s)	263, 1692
BsaAI	YACGTR	2 cut(s)	910, 1216
BsaHI	GRCGYC	2 cut(s)	117, 1419
BsaI	GGTCTC	1 cut(s)	422
BsaJI	CCNNGG	2 cut(s)	327, 1475
BsaWI	WCCGGW	2 cut(s)	137, 1730
BsaXI	ACNNNNNCTCC	2 cut(s)	758, 788
Bsc4I	CCNNNNNNNGG	3 cut(s)	112, 262, 1334
Bse118I	RCCGGY	2 cut(s)	1388, 1730
Bse1I	ACTGG	4 cut(s)	666, 850, 1231, 1609
Bse3DI	GCAATG	2 cut(s)	693, 1288
BseBI	CCWGG	1 cut(s)	376
BseDI	CCNNGG	2 cut(s)	327, 1475
BseGI	GGATG	3 cut(s)	102, 1311, 1547
BseLI	CCNNNNNNNGG	3 cut(s)	112, 262, 1334
BseMI	GCAATG	2 cut(s)	693, 1288
BseMII	CTCAG	4 cut(s)	1068, 1182, 1662, 1668
BseNI	ACTGG	4 cut(s)	666, 850, 1231, 1609
BseSI	GKGCMC	1 cut(s)	1221
BseYI	CCCAGC	1 cut(s)	1616
BsgI	GTGCAG	3 cut(s)	356, 670, 1341
BshFI	GGCC	7 cut(s)	5, 185, 665, 763, 1122, 1338, 1474
BshNI	GGYRCC	2 cut(s)	116, 1418
BshTI	ACCGGT	1 cut(s)	1730
BsiHKAI	GWGCWC	3 cut(s)	456, 756, 1221
BsiHKCI	CYCGRG	1 cut(s)	1092
BsiSI	CCGG	8 cut(s)	138, 263, 764, 1257, 1335, 1389, 1691, 1731
BslFI	GGGAC	2 cut(s)	1633, 1650
BslI	CCNNNNNNNGG	3 cut(s)	112, 262, 1334
BsmAI	GTCTC	2 cut(s)	422, 1089
BsmFI	GGGAC	2 cut(s)	1633, 1650
BsmI	GAATGC	1 cut(s)	1172
BsnI	GGCC	7 cut(s)	5, 185, 665, 763, 1122, 1338, 1474
Bso31I	GGTCTC	1 cut(s)	422
BsoBI	CYCGRG	1 cut(s)	1092
Bsp1286I	GDGCHC	3 cut(s)	456, 756, 1221
Bsp143I	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
Bsp1720I	GCTNAGC	1 cut(s)	536
Bsp19I	CCATGG	1 cut(s)	1475
BspACI	CCGC	7 cut(s)	332, 543, 782, 872, 926, 1070, 1180
BspANI	GGCC	7 cut(s)	5, 185, 665, 763, 1122, 1338, 1474
BspCNI	CTCAG	4 cut(s)	1067, 1181, 1663, 1669
BspHI	TCATGA	1 cut(s)	583
BspLI	GGNNCC	3 cut(s)	118, 1159, 1420
BspMAI	CTGCAG	1 cut(s)	801
BspPI	GGATC	2 cut(s)	480, 1393
BspQI	GCTCTTC	1 cut(s)	86
BspT107I	GGYRCC	2 cut(s)	116, 1418
BspTNI	GGTCTC	1 cut(s)	422
BsrDI	GCAATG	2 cut(s)	693, 1288
BsrFI	RCCGGY	2 cut(s)	1388, 1730
BsrI	ACTGG	4 cut(s)	666, 850, 1231, 1609
BssAI	RCCGGY	2 cut(s)	1388, 1730
BssECI	CCNNGG	2 cut(s)	327, 1475
BssMI	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
BssNAI	GTATAC	1 cut(s)	1686
BssNI	GRCGYC	2 cut(s)	117, 1419
BssSI	CACGAG	2 cut(s)	110, 458
BssT1I	CCWWGG	1 cut(s)	1475
Bst1107I	GTATAC	1 cut(s)	1686
Bst2BI	CACGAG	2 cut(s)	110, 458
Bst2UI	CCWGG	1 cut(s)	376
Bst4CI	ACNGT	6 cut(s)	355, 422, 862, 1063, 1264, 1498
Bst6I	CTCTTC	2 cut(s)	86, 887
BstACI	GRCGYC	2 cut(s)	117, 1419
BstBAI	YACGTR	2 cut(s)	910, 1216
BstC8I	GCNNGC	4 cut(s)	513, 1120, 1437, 1612
BstDEI	CTNAG	7 cut(s)	393, 536, 1054, 1148, 1168, 1671, 1677
BstDSI	CCRYGG	2 cut(s)	327, 1475
BstF5I	GGATG	3 cut(s)	102, 1311, 1547
BstH2I	RGCGCY	4 cut(s)	120, 411, 1422, 1617
BstHHI	GCGC	5 cut(s)	119, 410, 1421, 1616, 1753
BstKTI	GATC	6 cut(s)	235, 475, 672, 1165, 1401, 1503
BstMAI	GTCTC	2 cut(s)	422, 1089
BstMBI	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 878, 1064, 1216, 1622
BstNI	CCWGG	1 cut(s)	376
BstNSI	RCATGY	1 cut(s)	515
BstSCI	CCNGG	3 cut(s)	261, 374, 1690
BstSFI	CTRYAG	1 cut(s)	797
BstSLI	GKGCMC	1 cut(s)	1221
BstV2I	GAAGAC	1 cut(s)	798
BstX2I	RGATCY	1 cut(s)	472
BstYI	RGATCY	1 cut(s)	472
BstZ17I	GTATAC	1 cut(s)	1686
BsuRI	GGCC	7 cut(s)	5, 185, 665, 763, 1122, 1338, 1474
BtgI	CCRYGG	2 cut(s)	327, 1475
BtrI	CACGTC	3 cut(s)	298, 952, 1638
BtsCI	GGATG	3 cut(s)	102, 1311, 1547
BtsI	GCAGTG	2 cut(s)	1011, 1353
BtsIMutI	CAGTG	6 cut(s)	311, 843, 1011, 1353, 1602, 1668
Cac8I	GCNNGC	4 cut(s)	513, 1120, 1437, 1612
CaiI	CAGNNNCTG	2 cut(s)	1067, 1616
CciI	TCATGA	1 cut(s)	583
CfoI	GCGC	5 cut(s)	119, 410, 1421, 1616, 1753
Cfr10I	RCCGGY	2 cut(s)	1388, 1730
Cfr13I	GGNCC	2 cut(s)	1121, 1158
Csp6I	GTAC	3 cut(s)	484, 659, 1198
CspAI	ACCGGT	1 cut(s)	1730
CviQI	GTAC	3 cut(s)	484, 659, 1198
DdeI	CTNAG	7 cut(s)	393, 536, 1054, 1148, 1168, 1671, 1677
DinI	GGCGCC	2 cut(s)	118, 1420
DpnI	GATC	6 cut(s)	234, 474, 671, 1164, 1400, 1502
DpnII	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
EaeI	YGGCCR	4 cut(s)	3, 663, 761, 1336
Eam1104I	CTCTTC	2 cut(s)	86, 887
EarI	CTCTTC	2 cut(s)	86, 887
EciI	GGCGGA	2 cut(s)	532, 797
Ecl136II	GAGCTC	1 cut(s)	454
Eco130I	CCWWGG	1 cut(s)	1475
Eco147I	AGGCCT	1 cut(s)	185
Eco24I	GRGCYC	1 cut(s)	456
Eco31I	GGTCTC	1 cut(s)	422
Eco32I	GATATC	1 cut(s)	1525
Eco47I	GGWCC	1 cut(s)	1158
Eco53kI	GAGCTC	1 cut(s)	454
Eco57I	CTGAAG	1 cut(s)	389
Eco88I	CYCGRG	1 cut(s)	1092
EcoICRI	GAGCTC	1 cut(s)	454
EcoRII	CCWGG	1 cut(s)	374
EcoRV	GATATC	1 cut(s)	1525
EcoT14I	CCWWGG	1 cut(s)	1475
EcoT38I	GRGCYC	1 cut(s)	456
EgeI	GGCGCC	2 cut(s)	118, 1420
EheI	GGCGCC	2 cut(s)	118, 1420
ErhI	CCWWGG	1 cut(s)	1475
FalI	AAGNNNNNCTT	4 cut(s)	65, 97, 1436, 1468
FaqI	GGGAC	2 cut(s)	1633, 1650
FauI	CCCGC	1 cut(s)	919
FbaI	TGATCA	2 cut(s)	669, 1500
FblI	GTMKAC	1 cut(s)	1685
FokI	GGATG	3 cut(s)	89, 1298, 1554
FriOI	GRGCYC	1 cut(s)	456
FspBI	CTAG	2 cut(s)	1017, 1088
GlaI	GCGC	5 cut(s)	118, 409, 1420, 1615, 1752
GsaI	CCCAGC	1 cut(s)	1620
HaeII	RGCGCY	4 cut(s)	120, 411, 1422, 1617
HaeIII	GGCC	7 cut(s)	5, 185, 665, 763, 1122, 1338, 1474
HapII	CCGG	8 cut(s)	138, 263, 764, 1257, 1335, 1389, 1691, 1731
HhaI	GCGC	5 cut(s)	119, 410, 1421, 1616, 1753
Hin1I	GRCGYC	2 cut(s)	117, 1419
Hin6I	GCGC	5 cut(s)	117, 408, 1419, 1614, 1751
HinP1I	GCGC	5 cut(s)	117, 408, 1419, 1614, 1751
HincII	GTYRAC	1 cut(s)	1267
HindII	GTYRAC	1 cut(s)	1267
HinfI	GANTC	6 cut(s)	38, 1205, 1225, 1331, 1378, 1413
HpaII	CCGG	8 cut(s)	138, 263, 764, 1257, 1335, 1389, 1691, 1731
HphI	GGTGA	7 cut(s)	127, 251, 343, 605, 638, 1088, 1645
Hpy166II	GTNNAC	7 cut(s)	721, 748, 949, 1000, 1219, 1267, 1686
Hpy188I	TCNGA	5 cut(s)	37, 166, 868, 1162, 1429
Hpy188III	TCNNGA	6 cut(s)	322, 358, 584, 673, 1396, 1410
Hpy8I	GTNNAC	7 cut(s)	721, 748, 949, 1000, 1219, 1267, 1686
Hpy99I	CGWCG	5 cut(s)	170, 173, 359, 452, 1034
HpyAV	CCTTC	6 cut(s)	266, 286, 397, 1235, 1474, 1636
HpyCH4III	ACNGT	6 cut(s)	355, 422, 862, 1063, 1264, 1498
HpyCH4IV	ACGT	6 cut(s)	297, 909, 951, 1215, 1460, 1637
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 878, 1064, 1216, 1622
HpyF3I	CTNAG	7 cut(s)	393, 536, 1054, 1148, 1168, 1671, 1677
HpySE526I	ACGT	6 cut(s)	297, 909, 951, 1215, 1460, 1637
Hsp92I	GRCGYC	2 cut(s)	117, 1419
HspAI	GCGC	5 cut(s)	117, 408, 1419, 1614, 1751
KasI	GGCGCC	2 cut(s)	116, 1418
Ksp22I	TGATCA	2 cut(s)	669, 1500
Kzo9I	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
LguI	GCTCTTC	1 cut(s)	86
LmnI	GCTCC	3 cut(s)	221, 459, 545
LweI	GCATC	1 cut(s)	1401
MaeI	CTAG	2 cut(s)	1017, 1088
MaeII	ACGT	6 cut(s)	297, 909, 951, 1215, 1460, 1637
MaeIII	GTNAC	7 cut(s)	133, 309, 811, 1296, 1307, 1351, 1633
MalI	GATC	6 cut(s)	234, 474, 671, 1164, 1400, 1502
MboI	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
MboII	GAAGA	9 cut(s)	73, 79, 337, 340, 391, 798, 904, 1267, 1354
MflI	RGATCY	1 cut(s)	472
MhlI	GDGCHC	3 cut(s)	456, 756, 1221
MlsI	TGGCCA	2 cut(s)	5, 665
MluCI	AATT	5 cut(s)	50, 305, 644, 1303, 1656
MluNI	TGGCCA	2 cut(s)	5, 665
Mly113I	GGCGCC	2 cut(s)	117, 1419
MlyI	GAGTC	1 cut(s)	1199
MmeI	TCCRAC	3 cut(s)	189, 846, 1452
Mox20I	TGGCCA	2 cut(s)	5, 665
MroXI	GAANNNNTTC	1 cut(s)	80
MscI	TGGCCA	2 cut(s)	5, 665
MseI	TTAA	4 cut(s)	525, 570, 915, 1650
MslI	CAYNNNNRTG	2 cut(s)	332, 516
Msp20I	TGGCCA	2 cut(s)	5, 665
MspA1I	CMGCKG	1 cut(s)	1182
MspI	CCGG	8 cut(s)	138, 263, 764, 1257, 1335, 1389, 1691, 1731
MspR9I	CCNGG	3 cut(s)	263, 376, 1692
Mva1269I	GAATGC	1 cut(s)	1172
MvaI	CCWGG	1 cut(s)	376
MwoI	GCNNNNNNNGC	9 cut(s)	191, 394, 760, 769, 796, 878, 1064, 1216, 1622
NarI	GGCGCC	2 cut(s)	117, 1419
NciI	CCSGG	2 cut(s)	263, 1692
NcoI	CCATGG	1 cut(s)	1475
NdeII	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
NlaIV	GGNNCC	3 cut(s)	118, 1159, 1420
NmuCI	GTSAC	6 cut(s)	133, 309, 811, 1307, 1351, 1633
NspI	RCATGY	1 cut(s)	515
OliI	CACNNNNGTG	1 cut(s)	332
PaeI	GCATGC	1 cut(s)	515
PagI	TCATGA	1 cut(s)	583
PceI	AGGCCT	1 cut(s)	185
PciSI	GCTCTTC	1 cut(s)	86
PcsI	WCGNNNNNNNCGW	5 cut(s)	117, 177, 444, 456, 465
PctI	GAATGC	1 cut(s)	1172
PdmI	GAANNNNTTC	1 cut(s)	80
PfeI	GAWTC	5 cut(s)	38, 1225, 1331, 1378, 1413
PflFI	GACNNNGTC	1 cut(s)	862
PfoI	TCCNGGA	1 cut(s)	374
PinAI	ACCGGT	1 cut(s)	1730
PleI	GAGTC	1 cut(s)	1199
PluTI	GGCGCC	2 cut(s)	120, 1422
PpsI	GAGTC	1 cut(s)	1199
Ppu21I	YACGTR	2 cut(s)	910, 1216
Psp124BI	GAGCTC	1 cut(s)	456
Psp1406I	AACGTT	1 cut(s)	1460
Psp6I	CCWGG	1 cut(s)	374
PspFI	CCCAGC	1 cut(s)	1616
PspGI	CCWGG	1 cut(s)	374
PspN4I	GGNNCC	3 cut(s)	118, 1159, 1420
PspPI	GGNCC	2 cut(s)	1121, 1158
PstI	CTGCAG	1 cut(s)	801
PstNI	CAGNNNCTG	2 cut(s)	1067, 1616
PsuI	RGATCY	1 cut(s)	472
PsyI	GACNNNGTC	1 cut(s)	862
RsaI	GTAC	3 cut(s)	485, 660, 1199
RsaNI	GTAC	3 cut(s)	484, 659, 1198
RseI	CAYNNNNRTG	2 cut(s)	332, 516
SacI	GAGCTC	1 cut(s)	456
SapI	GCTCTTC	1 cut(s)	86
SaqAI	TTAA	4 cut(s)	525, 570, 915, 1650
Sau3AI	GATC	6 cut(s)	232, 472, 669, 1162, 1398, 1500
Sau96I	GGNCC	2 cut(s)	1121, 1158
SchI	GAGTC	1 cut(s)	1199
ScrFI	CCNGG	3 cut(s)	263, 376, 1692
SduI	GDGCHC	3 cut(s)	456, 756, 1221
SfaNI	GCATC	1 cut(s)	1401
SfcI	CTRYAG	1 cut(s)	797
SfoI	GGCGCC	2 cut(s)	118, 1420
SinI	GGWCC	1 cut(s)	1158
SmiMI	CAYNNNNRTG	2 cut(s)	332, 516
SmlI	CTYRAG	1 cut(s)	731
SmoI	CTYRAG	1 cut(s)	731
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	5 cut(s)	50, 305, 644, 1303, 1656
SseBI	AGGCCT	1 cut(s)	185
SsiI	CCGC	7 cut(s)	332, 543, 782, 872, 926, 1070, 1180
SspDI	GGCGCC	2 cut(s)	116, 1418
SspMI	CTAG	2 cut(s)	1017, 1088
SstI	GAGCTC	1 cut(s)	456
StuI	AGGCCT	1 cut(s)	185
StyD4I	CCNGG	3 cut(s)	261, 374, 1690
StyI	CCWWGG	1 cut(s)	1475
TaaI	ACNGT	6 cut(s)	355, 422, 862, 1063, 1264, 1498
TaiI	ACGT	6 cut(s)	300, 912, 954, 1218, 1463, 1640
TaqII	GACCGA	1 cut(s)	698
TasI	AATT	5 cut(s)	50, 305, 644, 1303, 1656
TatI	WGTACW	1 cut(s)	658
TfiI	GAWTC	5 cut(s)	38, 1225, 1331, 1378, 1413
Tru1I	TTAA	4 cut(s)	525, 570, 915, 1650
Tru9I	TTAA	4 cut(s)	525, 570, 915, 1650
TscAI	CASTG	6 cut(s)	318, 850, 1018, 1360, 1609, 1675
TseFI	GTSAC	6 cut(s)	133, 309, 811, 1307, 1351, 1633
Tsp45I	GTSAC	6 cut(s)	133, 309, 811, 1307, 1351, 1633
TspDTI	ATGAA	4 cut(s)	73, 722, 728, 1261
TspRI	CASTG	6 cut(s)	318, 850, 1018, 1360, 1609, 1675
Tth111I	GACNNNGTC	1 cut(s)	862
VneI	GTGCAC	1 cut(s)	1217
VpaK11BI	GGWCC	1 cut(s)	1158
XceI	RCATGY	1 cut(s)	515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	109
XmiI	GTMKAC	1 cut(s)	1685
XmnI	GAANNNNTTC	1 cut(s)	80
XspI	CTAG	2 cut(s)	1017, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.