AT1G54050

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014615
Physical Location & Seq
Reverse (-)
20179357 .. 20180566
1210 bp
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UTR
Exon/CDS
Intron
AT1G54050.2

Sequence Viewer

Length: 468 bp
ATGAGTGCTGTTGCGATTAACCACTTTTTTGGCTTACCGGAGGCGATCGAGAAGCTAATTCTCCCGATTTCTCGTTCCGGCGAAAGTAATAACGAGAGTCGTGGAAGAGGAAGTAGCAATAATATCCCAATAGACATTTTGGAATCTCCCAAAGAATACATATTTTATCTCGATATCCCCGGAATTTCAAAATCAGATATCCAGGTTACAGTGGAGGAAGAGAGGACTTTAGTGATAAAGAGTAATGGGAAGAGGAAGAGAGATGATGATGAGAGTGAAGAAGGGTCTAAGTATATTAGACTCGAGAGGAGACTTGCTCAGAATTTGGTTAAGAAGTTCCGGTTACCAGAAGATGCTGATATGGCTTCTGTAACGGCTAAATATCAAGAAGGTGTTTTGACAGTTGTTATCAAGAAGCTACCGCCACAGCCGCCGAAACCTAAGACTGTTCAAATCGCTGTTTCTTGA

Protein Analysis

155

Amino Acids

17.36

Weight (kDa)

7.88

Isoelectric Point (pI)

58.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 45 - 152 8.2e-21 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54050 AT1G54050
fragaria_vesca FvH4_6g35450
malus_domestica MD17G1151000.v1.1
prunus_persica Prupe.3G034800_v2.0.a1
pyrus_communis pycom17g14430
rosa_chinensis RchiOBHm_Chr2g0147001
rosa_laevigata RLG00000020237
rosa_multiflora Rmu_co8216844.1_g000001
rosa_roxburghii Rroxscaffold_2G00099770
rosa_rugosa Rorug02G0397900
rosa_samantha Rh2AG454900 Rh2BG467400 Rh2CG442300 Rh2DG476400
rosa_wichuraiana Rw2G037110 Rw2G037210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 422, 431
AcsI RAATTY 2 cut(s) 183, 322
AgsI TTSAA 2 cut(s) 189, 452
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 2 cut(s) 55, 418
AluI AGCT 2 cut(s) 55, 418
Alw26I GTCTC 1 cut(s) 304
Ama87I CYCGRG 1 cut(s) 302
ApoI RAATTY 2 cut(s) 183, 322
AsuC2I CCSGG 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 302
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 203
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 304
BisI GCNGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 432
Bme1390I CCNGG 2 cut(s) 180, 203
BmeT110I CYCGRG 1 cut(s) 302
BmrFI CCNGG 2 cut(s) 180, 203
BmsI GCATC 1 cut(s) 343
BplI GAGNNNNNCTC 2 cut(s) 301, 333
BpuMI CCSGG 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 2 cut(s) 37, 339
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 332
BseRI GAGGAG 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 48
BsiEI CGRYCG 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 4 cut(s) 38, 78, 180, 340
BsmAI GTCTC 1 cut(s) 304
BsoBI CYCGRG 1 cut(s) 302
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 2 cut(s) 422, 431
BspCNI CTCAG 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 211, 403, 448
Bst6I CTCTTC 4 cut(s) 100, 213, 245, 251
BstDEI CTNAG 3 cut(s) 288, 318, 441
BstEII GGTNACC 1 cut(s) 342
BstKTI GATC 1 cut(s) 48
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 45
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 362, 430
BstNI CCWGG 1 cut(s) 203
BstPI GGTNACC 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 178, 201
BstXI CCANNNNNNTGG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 216
CviJI RGCY 6 cut(s) 33, 55, 365, 377, 418, 430
CviKI_1 RGCY 6 cut(s) 33, 55, 365, 377, 418, 430
DdeI CTNAG 3 cut(s) 288, 318, 441
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
Eam1104I CTCTTC 4 cut(s) 100, 213, 245, 251
EarI CTCTTC 4 cut(s) 100, 213, 245, 251
Eco32I GATATC 2 cut(s) 175, 199
Eco88I CYCGRG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 342
EcoO65I GGTNACC 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 2 cut(s) 175, 199
FaiI YATR 3 cut(s) 161, 294, 362
Fnu4HI GCNGC 1 cut(s) 431
Fsp4HI GCNGC 1 cut(s) 431
GluI GCNGC 1 cut(s) 431
HapII CCGG 4 cut(s) 38, 78, 180, 340
HinfI GANTC 3 cut(s) 97, 143, 300
HpaII CCGG 4 cut(s) 38, 78, 180, 340
Hpy188I TCNGA 2 cut(s) 196, 321
Hpy188III TCNNGA 7 cut(s) 49, 64, 170, 304, 386, 412, 465
HpyAV CCTTC 2 cut(s) 275, 383
HpyCH4III ACNGT 3 cut(s) 211, 403, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 430
HpyF3I CTNAG 3 cut(s) 288, 318, 441
Kzo9I GATC 1 cut(s) 45
LpnPI CCDG 7 cut(s) 51, 91, 188, 193, 215, 353, 360
LweI GCATC 1 cut(s) 343
MaeIII GTNAC 3 cut(s) 205, 342, 370
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 6 cut(s) 117, 230, 262, 268, 290, 362
MluCI AATT 3 cut(s) 57, 183, 322
MlyI GAGTC 2 cut(s) 106, 294
MnlI CCTC 6 cut(s) 34, 101, 208, 216, 246, 300
MseI TTAA 2 cut(s) 18, 330
MspI CCGG 4 cut(s) 38, 78, 180, 340
MspR9I CCNGG 2 cut(s) 180, 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 362, 430
NciI CCSGG 1 cut(s) 180
NdeII GATC 1 cut(s) 45
PaeR7I CTCGAG 1 cut(s) 302
PfeI GAWTC 1 cut(s) 143
PkrI GCNGC 1 cut(s) 432
Ple19I CGATCG 1 cut(s) 48
PleI GAGTC 2 cut(s) 105, 294
PpsI GAGTC 2 cut(s) 105, 294
Psp6I CCWGG 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 342
PspGI CCWGG 1 cut(s) 201
PvuI CGATCG 1 cut(s) 48
SaqAI TTAA 2 cut(s) 18, 330
SatI GCNGC 1 cut(s) 431
Sau3AI GATC 1 cut(s) 45
SchI GAGTC 2 cut(s) 106, 294
ScrFI CCNGG 2 cut(s) 180, 203
SetI ASST 5 cut(s) 57, 207, 394, 420, 442
SfaNI GCATC 1 cut(s) 343
Sfr274I CTCGAG 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 3 cut(s) 57, 183, 322
SsiI CCGC 2 cut(s) 422, 431
StyD4I CCNGG 2 cut(s) 178, 201
TaaI ACNGT 3 cut(s) 211, 403, 448
TaqI TCGA 3 cut(s) 48, 171, 303
TasI AATT 3 cut(s) 57, 183, 322
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 2 cut(s) 18, 330
Tru9I TTAA 2 cut(s) 18, 330
TscAI CASTG 1 cut(s) 216
TspRI CASTG 1 cut(s) 216
XapI RAATTY 2 cut(s) 183, 322
XhoI CTCGAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.