MD17G1151000.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014615
Physical Location & Seq
Forward (+)
13922992 .. 13924497
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1151000.v1.1.491

Sequence Viewer

Length: 471 bp
ATGAGCCGAGCAGCGGATTTGAGTGCGTTCGAAGTGGTGAACCAGCTGCTGAACTTCCCAGAGGCCATTGAGAAGCTCATGTTTCCGTCTCGGGCTCACGAGACCGGTAACGATAACAAAGGTGTGTCCAGCATTCCGGTTGACATTTTGGACACTCCCAAGGAGTACATATTTTTCTTGGATGTGCCTGGCCTATCCAAATCTGACATTCAGGTGACGGTTGAAGACGAGAACACTCTGGTGATCCGGAGCAATGGGAAGAGAAAGCGCGAAGACGGTGAGGAGGAGGGGTGCAAGTATATCAGGCTGGAGAGGAGAGCGGCCCAGAAGCTACTGAGGAAGTTCCGGTTGCCCGACAATGCCAATGTAGGAGCCATTAATGCGAAATGTGAGAACGGGGTTCTGACTGTGGTGGTTGAGAAGCTTCCTCCCCCTCCCAAGCCCAAGACTGTTAAAGTCTCCATCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

157

Amino Acids

17.36

Weight (kDa)

7.72

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 48 - 153 4.8e-21 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54050 AT1G54050
fragaria_vesca FvH4_6g35450
malus_domestica MD17G1151000.v1.1
prunus_persica Prupe.3G034800_v2.0.a1
pyrus_communis pycom17g14430
rosa_chinensis RchiOBHm_Chr2g0147001
rosa_laevigata RLG00000020237
rosa_multiflora Rmu_co8216844.1_g000001
rosa_roxburghii Rroxscaffold_2G00099770
rosa_rugosa Rorug02G0397900
rosa_samantha Rh2AG454900 Rh2BG467400 Rh2CG442300 Rh2DG476400
rosa_wichuraiana Rw2G037110 Rw2G037210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 320
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 2 cut(s) 14, 320
AclWI GGATC 1 cut(s) 238
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 13
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 1 cut(s) 187
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 4 cut(s) 46, 76, 331, 424
AluI AGCT 4 cut(s) 46, 76, 331, 424
Alw26I GTCTC 3 cut(s) 93, 95, 463
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 90
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 3 cut(s) 63, 190, 321
ApeKI GCWGC 2 cut(s) 11, 46
AseI ATTAAT 1 cut(s) 378
AsiGI ACCGGT 1 cut(s) 104
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 4 cut(s) 49, 226, 253, 290
AsuII TTCGAA 1 cut(s) 30
AvaI CYCGRG 1 cut(s) 90
BanII GRGCYC 1 cut(s) 97
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 231, 279
BbvI GCAGC 2 cut(s) 23, 33
BccI CCATC 1 cut(s) 470
BciT130I CCWGG 1 cut(s) 189
BcoDI GTCTC 3 cut(s) 93, 95, 463
BisI GCNGC 3 cut(s) 12, 47, 321
BlsI GCNGC 3 cut(s) 13, 48, 322
Bme1390I CCNGG 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 189
BpiI GAAGAC 2 cut(s) 231, 279
BpmI CTGGAG 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 4 cut(s) 104, 136, 246, 345
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse118I RCCGGY 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 259
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 326
BseRI GAGGAG 3 cut(s) 296, 299, 328
BseXI GCAGC 2 cut(s) 23, 33
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 65, 192, 323
BshTI ACCGGT 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 4 cut(s) 105, 137, 247, 346
BslI CCNNNNNNNGG 1 cut(s) 13
BsmAI GTCTC 3 cut(s) 93, 95, 463
BsmBI CGTCTC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 3 cut(s) 65, 192, 323
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 1 cut(s) 243
BspACI CCGC 2 cut(s) 14, 320
BspANI GGCC 3 cut(s) 65, 192, 323
BspCNI CTCAG 1 cut(s) 327
BspEI TCCGGA 1 cut(s) 246
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 373
BspPI GGATC 1 cut(s) 238
BspT104I TTCGAA 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 4 cut(s) 220, 278, 409, 451
Bst6I CTCTTC 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 1 cut(s) 246
BstMAI GTCTC 3 cut(s) 93, 95, 463
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 23, 33
BstV2I GAAGAC 2 cut(s) 231, 279
BsuRI GGCC 3 cut(s) 65, 192, 323
BtsCI GGATG 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 166
CspAI ACCGGT 1 cut(s) 104
CviAII CATG 1 cut(s) 79
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 1 cut(s) 335
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 97
Eco31I GGTCTC 1 cut(s) 95
Eco88I CYCGRG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 1 cut(s) 82
FaiI YATR 3 cut(s) 80, 170, 300
FatI CATG 1 cut(s) 78
Fnu4HI GCNGC 3 cut(s) 12, 47, 321
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 3 cut(s) 12, 47, 321
GlaI GCGC 1 cut(s) 269
GluI GCNGC 3 cut(s) 12, 47, 321
GsuI CTGGAG 1 cut(s) 329
HaeIII GGCC 3 cut(s) 65, 192, 323
HapII CCGG 4 cut(s) 105, 137, 247, 346
HhaI GCGC 1 cut(s) 270
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 422
HpaII CCGG 4 cut(s) 105, 137, 247, 346
HphI GGTGA 4 cut(s) 49, 226, 253, 290
Hpy166II GTNNAC 2 cut(s) 40, 142
Hpy188I TCNGA 2 cut(s) 205, 405
Hpy188III TCNNGA 3 cut(s) 98, 247, 468
Hpy8I GTNNAC 2 cut(s) 40, 142
HpyCH4III ACNGT 4 cut(s) 220, 278, 409, 451
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 1 cut(s) 335
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 246
Kzo9I GATC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 249, 371
Lsp1109I GCAGC 2 cut(s) 23, 33
MaeIII GTNAC 2 cut(s) 107, 214
MalI GATC 1 cut(s) 245
MbiI CCGCTC 1 cut(s) 320
MboI GATC 1 cut(s) 243
MboII GAAGA 3 cut(s) 236, 271, 284
MhlI GDGCHC 1 cut(s) 97
MnlI CCTC 8 cut(s) 55, 274, 277, 280, 306, 330, 438, 444
MroI TCCGGA 1 cut(s) 246
MseI TTAA 2 cut(s) 378, 453
MslI CAYNNNNRTG 2 cut(s) 212, 239
MspA1I CMGCKG 2 cut(s) 14, 46
MspI CCGG 4 cut(s) 105, 137, 247, 346
MspR9I CCNGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 132
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 380
NdeII GATC 1 cut(s) 243
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 214
NspV TTCGAA 1 cut(s) 30
OliI CACNNNNGTG 1 cut(s) 239
PctI GAATGC 1 cut(s) 132
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 3 cut(s) 13, 48, 322
PshBI ATTAAT 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 2 cut(s) 212, 239
SaqAI TTAA 2 cut(s) 378, 453
SatI GCNGC 3 cut(s) 12, 47, 321
Sau3AI GATC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 97
SetI ASST 6 cut(s) 48, 78, 124, 216, 333, 426
SfuI TTCGAA 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 212, 239
SsiI CCGC 2 cut(s) 14, 320
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 4 cut(s) 220, 278, 409, 451
TaqI TCGA 1 cut(s) 30
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 378, 453
Tru9I TTAA 2 cut(s) 378, 453
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 11, 46
Tsp45I GTSAC 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 75
VspI ATTAAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.