FvH4_6g35450

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014615
Physical Location & Seq
Forward (+)
27957697 .. 27958639
943 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35450.t1

Sequence Viewer

Length: 450 bp
ATGGACTTGGAAGCAGTAGTGAGCCACCTGCTCAATATTCCGGAGACCATCCCCACCTACGTGCATCCTTCTCGGGCTGCTCATGGAACCAAAGAGAACCAAGGTGCCTCCACCATTCCGGTCGACATATTGGACTCTGCCAAGGACTACACCTTTTACTTGGACGTTCCTGGCTTGTCCAAGTCTGATATTCAGGTAACCGTTGAAGATGAGAACAATCTGGTGATTCGGAGCAACGGGAAAAGGAAGCGCGAAGATGGAGAGGAGGAAGGATGCAAGTATATTAGGCTCGAGAGGAAGGCACCACAGAAGATGCTGAGGAAGTTTCGATTGCCTGAGAATGCGAATGTTGGAGCCATCTCTGCCAAATGCGAAAACGGAGTTCTGACTGTGGTTGTTCAGAAGCATCCACCTTCCAAGCCCAAAACGGTTGCAGTTAACATCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

150

Amino Acids

16.48

Weight (kDa)

7.78

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 42 - 146 2e-20 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54050 AT1G54050
fragaria_vesca FvH4_6g35450
malus_domestica MD17G1151000.v1.1
prunus_persica Prupe.3G034800_v2.0.a1
pyrus_communis pycom17g14430
rosa_chinensis RchiOBHm_Chr2g0147001
rosa_laevigata RLG00000020237
rosa_multiflora Rmu_co8216844.1_g000001
rosa_roxburghii Rroxscaffold_2G00099770
rosa_rugosa Rorug02G0397900
rosa_samantha Rh2AG454900 Rh2BG467400 Rh2CG442300 Rh2DG476400
rosa_wichuraiana Rw2G037110 Rw2G037210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 36
Acc36I ACCTGC 1 cut(s) 36
AccB1I GGYRCC 2 cut(s) 104, 301
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 40
AgsI TTSAA 1 cut(s) 206
AjnI CCWGG 1 cut(s) 169
AleI CACNNNNGTG 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 38
Ama87I CYCGRG 2 cut(s) 72, 290
Aor13HI TCCGGA 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 77
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 2 cut(s) 72, 290
BanI GGYRCC 2 cut(s) 104, 301
BbvCI CCTCAGC 1 cut(s) 317
BbvI GCAGC 1 cut(s) 64
BccI CCATC 3 cut(s) 56, 251, 365
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 171
BmeT110I CYCGRG 2 cut(s) 72, 290
BmiI GGNNCC 4 cut(s) 88, 106, 303, 355
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 4 cut(s) 73, 263, 303, 415
Bpu10I CCTNAGC 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 100, 141
BsaWI WCCGGW 2 cut(s) 40, 118
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 100, 141
BseGI GGATG 4 cut(s) 48, 64, 278, 406
BseMII CTCAG 2 cut(s) 308, 327
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 252
Bsh1285I CGRYCG 1 cut(s) 123
BshNI GGYRCC 2 cut(s) 104, 301
BsiEI CGRYCG 1 cut(s) 123
BsiHKCI CYCGRG 2 cut(s) 72, 290
BsiSI CCGG 2 cut(s) 41, 119
BsmAI GTCTC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 346
Bso31I GGTCTC 1 cut(s) 38
BsoBI CYCGRG 2 cut(s) 72, 290
Bsp13I TCCGGA 1 cut(s) 40
BspCNI CTCAG 2 cut(s) 309, 328
BspEI TCCGGA 1 cut(s) 40
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 88, 106, 303, 355
BspMI ACCTGC 1 cut(s) 36
BspT107I GGYRCC 2 cut(s) 104, 301
BspTNI GGTCTC 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 100, 141
BssT1I CCWWGG 2 cut(s) 100, 141
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 202, 391, 430
BstBAI YACGTR 1 cut(s) 61
BstDEI CTNAG 2 cut(s) 317, 336
BstEII GGTNACC 1 cut(s) 196
BstF5I GGATG 4 cut(s) 48, 64, 278, 406
BstFNI CGCG 1 cut(s) 252
BstHHI GCGC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 38
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 171
BstPI GGTNACC 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 169
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 64
BtsCI GGATG 4 cut(s) 48, 64, 278, 406
BveI ACCTGC 1 cut(s) 36
CfoI GCGC 1 cut(s) 252
CviAII CATG 1 cut(s) 83
CviJI RGCY 6 cut(s) 24, 77, 174, 289, 356, 421
CviKI_1 RGCY 6 cut(s) 24, 77, 174, 289, 356, 421
DdeI CTNAG 2 cut(s) 317, 336
Eco130I CCWWGG 2 cut(s) 100, 141
Eco31I GGTCTC 1 cut(s) 38
Eco88I CYCGRG 2 cut(s) 72, 290
Eco91I GGTNACC 1 cut(s) 196
EcoO65I GGTNACC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 100, 141
ErhI CCWWGG 2 cut(s) 100, 141
FaeI CATG 1 cut(s) 86
FaiI YATR 3 cut(s) 84, 128, 282
FatI CATG 1 cut(s) 82
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 4 cut(s) 35, 51, 285, 393
Fsp4HI GCNGC 1 cut(s) 78
GlaI GCGC 1 cut(s) 251
GluI GCNGC 1 cut(s) 78
HapII CCGG 2 cut(s) 41, 119
HhaI GCGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 86
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 2 cut(s) 124, 439
HindII GTYRAC 2 cut(s) 124, 439
HinfI GANTC 2 cut(s) 134, 226
HpaI GTTAAC 1 cut(s) 439
HpaII CCGG 2 cut(s) 41, 119
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 124, 439
Hpy188I TCNGA 4 cut(s) 187, 231, 387, 402
Hpy188III TCNNGA 3 cut(s) 41, 292, 447
Hpy8I GTNNAC 2 cut(s) 124, 439
HpyAV CCTTC 4 cut(s) 78, 263, 292, 423
HpyCH4III ACNGT 3 cut(s) 202, 391, 430
HpyCH4IV ACGT 2 cut(s) 60, 165
HpyCH4V TGCA 3 cut(s) 64, 276, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 2 cut(s) 317, 336
HpySE526I ACGT 2 cut(s) 60, 165
Hsp92II CATG 1 cut(s) 86
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 40
KspAI GTTAAC 1 cut(s) 439
LmnI GCTCC 2 cut(s) 231, 353
LpnPI CCDG 8 cut(s) 41, 54, 132, 156, 179, 183, 206, 348
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 4 cut(s) 73, 263, 303, 415
MaeII ACGT 2 cut(s) 60, 165
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 3 cut(s) 218, 266, 322
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 5 cut(s) 118, 256, 259, 288, 312
MroI TCCGGA 1 cut(s) 40
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 59
MspI CCGG 2 cut(s) 41, 119
MspR9I CCNGG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 362
NlaIII CATG 1 cut(s) 86
NlaIV GGNNCC 4 cut(s) 88, 106, 303, 355
OliI CACNNNNGTG 1 cut(s) 59
PaeR7I CTCGAG 1 cut(s) 290
PaqCI CACCTGC 1 cut(s) 36
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 169
PspEI GGTNACC 1 cut(s) 196
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 4 cut(s) 88, 106, 303, 355
RseI CAYNNNNRTG 1 cut(s) 59
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 1 cut(s) 78
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 171
SetI ASST 8 cut(s) 30, 59, 63, 106, 155, 168, 198, 415
SfaNI GCATC 4 cut(s) 73, 263, 303, 415
Sfr274I CTCGAG 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SspI AATATT 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 2 cut(s) 100, 141
TaaI ACNGT 3 cut(s) 202, 391, 430
TaiI ACGT 2 cut(s) 63, 168
TaqI TCGA 3 cut(s) 123, 291, 328
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TseI GCWGC 1 cut(s) 77
TspGWI ACGGA 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.